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981.
目的:研究大肠癌Lovo细胞上皮钙粘附素(E-Cadherin),神经钙粘附素(N-Cadherin)的表达及常用化学治疗药物对其表达的影响。方法:应用逆转录聚合酶链反应(RT-PCR)法检测大肠癌Lovo细胞E-Cad-herin,N-Cadherin的表达,并通过几种不同的化疗药物(顺铂、吡柔比星、丝裂霉素、5-FU)不同浓度和时间作用后,对E-Cadherin,N-Cadherin表达的影响。结果:不同化疗药物对大肠癌Lovo细胞N=Cadherin表达无影响,高浓度顺铂和高浓度吡柔比星二组出现-Cadherin表达,其它各组未见表达。结论:大肠癌常用的化疗药物无论低浓度持续用药,还是高浓度短时间用药,对大肠癌Lovo细胞N-Cadherin的表达无抑制作用,说明上述药物不能通过抑制N—Cadherin的表达,而起到抗癌作用。高浓度顺铂和吡柔比星二组显示E-Cadherin的表达,表现出抑癌的作用,从而提示在大肠癌化疗时,顺铂和吡柔比星更适合于高浓度短时间应用。 相似文献
982.
目的:检测各种肾小球疾病患者血清中抗中性粒细胞胞浆抗体(ANCA)并探讨其临床意义。方法:应用间接免疫荧光法(IIF)检测20例正常人及131例各种肾小球疾病患者血清中ANCA。结果:131例患者中有11例ANCA阳性性(阳性率为8.4%),且均为P-ANCA阳性,而20例正常对照组无1例阳性(P<0.05)。ANCA阳性中以新月体性肾炎、狼疮性肾炎、紫癜性肾炎的阳性率较高,分别为67%、25%、16.7%。新月体性肾炎与IgA肾病等原发性肾小球疾病组比较有显著性差异(P<0.05)。结论:ANCA在各种肾小球疾病中并不少见,尤其是新月体性肾炎、狼疮性肾炎、紫癜性肾炎的阳性率较高,推测ANCA可能在这些疾病的发病机理中起了一定的作用。 相似文献
983.
Simple measurement of spinal cord evoked potential: a valuable data source in the rat spinal cord injury model. 总被引:1,自引:0,他引:1
Jong-Pil Park Ki-Jeong Kim Ji Hoon Phi Chul-Kee Park Jong-Hoon Kim Hyo Jin Kang Dohun Lee Kyu Hyun Han Kyu-Chang Wang Sun Ha Paek 《Journal of clinical neuroscience》2007,14(11):1099-1105
Measurement of spinal cord evoked potentials (SCEPs) is proposed as a means of predicting locomotion outcome in the rat spinal cord injury (SCI) model. Using 55 rats, three reproducible peak waves (waves I, II and III) were observed during stimulation at the C7 level with recording at the L1 epidural space. Hemisection at the T13 level showed three wave loss patterns: wave III loss only, loss of both wave II and III, and loss of all three waves. Defining an ideal SCI model as establishment of stable monoparesis or paraparesis, all animals in the wave II-III loss group showed favorable results. Histological data and electrophysiological properties allowed reasonable assumptions of wave origin: wave I from extrapyramidal tracts, wave II from the ventral corticospinal tract, and wave III from the dorsal corticospinal tract. Complete destruction of pyramidal tracts in both dorsal and ventral fibers was essential for long-term impairment of locomotion. 相似文献
984.
一些保肝药物对原代培养大鼠肝细胞糖原合成功能的影响 总被引:1,自引:0,他引:1
本文参照PO Seglen的方法并加以修改,建立了原代培养大鼠肝细胞糖原合成功能的测定体系。观察到联苯双酯既能使正常肝细胞合成糖原增加88%,又能保护肝细胞完全拮抗四氯化碳对其功能的损伤;银耳多糖能使四氯化碳对肝细胞糖原合成功能的损伤减轻57%;去甲斑蝥素10μg/ml能增加肝细胞糖原合成,浓度增加到100μg/ml时,此作用减弱,1000μg/ml则明显抑制糖原的合成,而且在10~100μg/ml浓度时,即能加强四氯化碳的损伤作用;100μg/ml CL1500和熊果酸二钠单独应用可增加肝细胞糖原合成,但与四氯化碳同时应用,反而加重对糖原合成的抑制作用。 相似文献
985.
Charlotte E Hotchkiss Cheng Wang William Slikker 《Journal of the American Association for Laboratory Animal Science》2007,46(6):21-28
Physiologic measurements in nonhuman primates usually are collected from animals that are chemically or physically restrained. Both types of restraint may affect the parameters measured, and those effects can vary with age. Heart rate, respiratory rate, oxygen saturation, expired CO2, blood pressure, temperature, blood glucose, hematocrit, and venous blood gasses were measured in rhesus monkeys that were either infused intravenously with ketamine for 24 h or were cage-housed and physically restrained for sample collection. The subjects were pregnant monkeys at gestational day 120 to 123, infants 5 to 6 d old, and infants 35 to 37 d old. Heart rate and blood pressure were lower in ketamine-treated monkeys than physically restrained monkeys. Heart rate was higher in infants than adults, whereas blood pressure was lower in infants. Respiratory rate was higher in infants than adults and higher in physically restrained infants than ketamine-sedated infants but was not affected by ketamine in pregnant adults. Hematocrit was decreased in older infants. In summary, both physical restraint and ketamine sedation altered several physiologic parameters in pregnant and infant rhesus macaques. Investigators should consider these effects when designing experiments and evaluating experimental outcomes in monkeys. 相似文献
986.
We detected the presence and distribution of HBcAg in the liver by immunohistochemistry (ABC method) and the presence of HBV-DNA in serum (spot hybridization) and anti-HBe in serum (ELISA) from 59 cases of hepatitis B hospitalized in our hospital, including 47 cases of CAH, 5 cases of CPH, and 7 cases of subacute fulminant hepatitis. 1. HBcAg in the liver was detected in 25 out of 47 cases (53%) of CAH, in 2 out of 5 cases of CPH and in 4 out of 7 cases of subacute fulminant hepatitis. The total percentage was 53% (31/59). 2. There was no positive correlation between HBV replication activity and liver disease activity (P greater than 0.05). Our results did not support the hypothesis that suggests a direct cytopathic effect of HBV. Oppositely, the fact was that the presence, the amount and the patterns of HBcAg in the liver, and the presence of HBV-DNA in serum were predominant in mild CAH compared with those in severe CAH, predominant in CAH without cirrhosis compared with those in CAH with cirrhosis. There was a tendency of inverse correlation between HBV replication activity and liver disease activity. The results above were in line with the concept that HBcAg expressed on the surface of infected hepatocytes may be relevant target for T lymphocyte cytotoxicity. The results have suggested that an immune response to HBV is present, leading to the destruction of most infected cells. 3. There was a positive correlation between HBV-DNA in serum and HBcAg in the liver (P less than 0.005), indicating that HBV-DNA in serum can represent HBV replication.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
987.
An elevated serum cholesterol level is a well known major risk factor for developing atherosclerosis in general, and for coronary heart disease in particular. There are many lipid lowering agents currently available. We used gemfibrozil in twenty hyperlipidemic cases who failed to response to diet control for three months. They were thirteen males and seven females with their ages ranging from thirty to eighty-one year-old. They included ten diabetes, one nephrotic syndrome and nine pure hyperlipidemic patients. All cases received gemfibrozil 600mg twice daily for 4-12 weeks. Gemfibrozil caused an increase in HDL cholesterol. The reductions in serum level of total cholesterol, total cholesterol/HDL cholesterol, and triglyceride were found. Only one case developed mild gastrointestinal side effect. There was no other major side effects. 相似文献
988.
989.
目的探讨交叉韧带重建术中可吸收挤压螺钉的使用方法和疗效。方法总结53例交叉韧带重建病例使用可吸收挤压螺钉的情况,在术中、术后并发症、术后康复、膝关节功能状况等方面进行回顾性分析。结果三例出现韧带切割现象。两例股骨侧螺钉拧入后导引针无法拔出。一例挤压螺钉断裂。术后Lysholm评分平均92.4±4.1。结论交叉韧带重建术中使用可吸收挤压螺钉固定的方法固定牢固,术后恢复快,利于早期康复。 相似文献
990.
本文用显微分光光度计对39例手术切除的不同组织学类型的人体肺癌标本癌细胞核的DNA含量进行了测量。其中高、中、低分化的鳞癌分别为3、6和5例;高、中、低分化的腺癌分别为3、5和5例;未分化癌大细胞型和小细胞型各为3例和6例。结果发现各类型肺癌的DNA含量均有统计学上的差异(P<0.01)。各DNA含量直方图的峰值部位及分布范围亦不同。从而提示了根据DNA含量的不同,可能有助于肺癌组织学类型及其分化程度的判别:而DNA含量直方图对肺癌类型的判别亦可能是有益的。 相似文献