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81.
82.
Takashi Sekine Keiko Fukutani Tomiko Motegi Hiroshi Hayakawa Takashi Tamura Shigeo Nagafuchi Yutaka Nakahori Yasuo Nakagome 《Journal of human genetics》1992,37(2):157-162
Summary Results of DNA study on two patients of gonadal dysgenesis with a 45,X/46,X,Ynf (non-fluorescent Y chromosome) karyotype are described. In one patient who developed gonadoblastoma, all 12 loci on the non-fluorescent part of Yq were detected. Another patient did not have gonadoblastoma at 20 years, and only the proximal 6 loci out of 12 were detected. 相似文献
83.
Morphogenesis of nonpolypoid colorectal adenomas and early carcinomas assessed by cell proliferation and apoptosis 总被引:4,自引:0,他引:4
Nomura M Watari J Yokota K Saitoh Y Obara T Kohgo Y 《Virchows Archiv : an international journal of pathology》2000,437(1):17-24
Nonpolypoid neoplasms, as well as ordinary polypoid tumours, are occasionally found in the colorectum. To clarify whether
cell kinetic status affects the macroscopic morphology of colorectal neoplasms, we investigated proliferative indices (PI),
apoptotic indices (AI), and the expression of apoptosis-related gene products. We examined 110 colorectal neoplasms comprised
of 36 polypoid, 38 flat elevated and 36 depressed tumours. According to WHO’s criteria these tumours consisted of 61 adenomas
with low grade dysplasia (LGD), 30 adenomas with high grade dysplasia (HGD) and 19 carcinomas with submucosal invasion. Apoptotic
cells were detected by TUNEL staining. Proliferating cells and apoptosis-related gene products were assessed by immunohistochemistry
for Ki-67, p53, Bcl-2, and Bax antigens. AI were closely associated with macroscopic morphology in adenomas but not in carcinomas.
PI were relatively constant among the three macroscopic types in adenomas and carcinomas. Median AI values of polypoid, flat
elevated and depressed tumours were 1.8%, 2.1% and 4.6% for adenomas with LGD, 0.8%, 2.4% and 6.2% for adenomas with HGD and
2.9%, 4.0% and 3.6% for carcinomas, respectively. Overall PI were significantly higher in carcinomas than in adenomas with
LGD, whereas AI were not different. Although the incidence of expression was significantly higher in carcinomas for p53 and
in adenomas for Bcl-2 than the others, the expression of apoptosis-related gene products (p53, Bcl-2 and Bax) was similar
among polypoid, flat elevated and depressed tumours. Macroscopic morphology of colorectal adenomas is determined by the apoptosis
not by proliferation, and high apoptosis found in depressed adenomas implies their low net growth.
Received: 1 July 1999 / Accepted: 17 January 2000 相似文献
84.
Nobuo Tsuda Paritosh Roy Chowdhury Tomayoshi Hayashi Masanobu Anami Masachika Iseki Shigehiko Koga Fukuzo Matsuya Hiroshi Kanetake Yutaka Saito Yoshio Horita 《Pathology international》1997,47(11):778-783
Primary renal angiosarcoma is very rare. To our knowledge, only 15 cases have been reported to date. A 77-year-old Japanese man with a unilateral kidney presented with massive hematuria followed by renal failure. A renal tumor was suspected and a left nephrectomy was performed. The histopathological diagnosis was angiosarcoma of the kidney. A hemorrhagic tumor measuring 10 × 5 cm and clotted blood was found in the modularly area. The atypical tumor cells had a sinusoidal and solid appearance, and showed Immunohistochemically positive reactions for some of the endothelial markers. The patient died about 21 months after the nephrectomy and the autopsy revealed massive metastases to the liver and retroperitoneum. One of the differential diagnoses of the case was anglomyolipoma, because the tumor cells were relatively bland in their histological appearance with entrapped fat cells in the pelvic area. Fifteen case reports with titles that included the term 'hemangiosarcoma/anglosarcoma', 'hemangioendothelloma/endothelloma' or 'vascular sarcoma' of the kidney were reviewed and compared to the present case. 相似文献
85.
Association of EGFR Gene Amplification and CDKN2 (p16/MTS1) Gene Deletion in Glioblastoma Multiforme 总被引:9,自引:0,他引:9
Yutaka Hayashi Keisuke Ueki reas Waha Otmar D. Wiestler David N. Louis reas von Deimlina 《Brain pathology (Zurich, Switzerland)》1997,7(3):871-875
Glioblastoma multiforme (GBM) can be divided into genetic subsets: approximately one-third of GBM, primarily in older adults, have EGFR amplification; another one-third, primarily in younger adults, have TP53 mutation. The majority of GBM also have homozygous deletions of the CDKN2 (p16/MTS1) gene, resulting in cell cycle deregulation and elevated proliferation indices. We evaluated the relationship between CDKN2 deletions and the GBM subsets as defined by EGFR amplification or TP53 mutation in 70 GBM. Twenty-eight cases (40%) had EGFR amplification, 21 (30%) had TP53 mutation, and 21 (30%) had neither change. CDKN2 deletions were present in 36 (51%) GBM. Of the 28 GBM with EGFR amplification, 20 (71%) had CDKN2 deletion (p = 0.0078). The remaining 16 cases with CDKN2 loss were divided between GBM with TP53 mutations (6 cases) and GBM with neither EGFR amplification nor TP53 mutation (10 cases). Thus, CDKN2 deletions occur twice as commonly in GBM with EGFR amplification (71%) than in GBM with TP53 mutation (29%). CDKN2 deletions occurred in GBM from patients somewhat older than those patients with GBM lacking CDKN2 deletion (mean age 53 vs. 48 years). Specifically among GBM with EGFR amplification, those with CDKN2 deletions also occurred in patients slightly older than those few GBM without CDKN2 deletions (mean age 55 vs. 51 years). The presence of CDKN2 deletions in most GBM with EGFR amplification and in generally older patients may provide one explanation for the potentially more aggressive nature of such tumors. 相似文献
86.
Atsuo Ogura Toshihiko Asano Junichiro Matsuda Minako Koura Masaro Nakagawa Hiroshi Kawaguchi Yutaka Yamaguchi 《Virchows Archiv : an international journal of pathology》1990,417(3):223-228
Summary Glomerular lesions in hereditary nephrotic mice (ICGN strain) were investigated by electron microscopy. The glomeruli of unaffected animals, which appeared normal by light microscopy, had developed an ultrastructural change in the glomerular capillary basement membrane (GCBM). There was a partial thickening of the GCBM with bilaminar splitting of the lamina densa and an electron-dense fibrillar material exhibiting cross-striations. In affected animals, light microscopy revealed a marked thickening of GCBM and an increase of mesangial matrix without cellular proliferaton. By electron microscopy, multilaminar splitting of the lamina densa in the thickened GCBMs and fusion of the epithelial foot processes were observed. In some severely affected animals, immune complex deposition was found in GCBM, but little if any was observed in other animals. In the end, the glomeruli were globally sclerosed. Our findings suggest that initial structural abnormalities in GCBM may play an important role in the onset and development of the disease, though subsequent events such as immune complex deposition would modify the disease. 相似文献
87.
PCR analysis of the Y chromosome long arm in azoospermic patients: evidence for a second locus required for spermatogenesis 总被引:20,自引:2,他引:20
Kobayashi Kazuhiro; Mlzuno Kunihiko; Hida Akiko; Komakl Rie; Tomita Keiko; Matsushita Ikumi; Namlki Mikio; Iwamoto Teruaki; Tamura Shohzoh; Minowada Shlgeru; Nakahori Yutaka 《Human molecular genetics》1994,3(11):1965-1967
We analyzed DNA from 63 Japanese men with either azoospermiaor severe oligospermia whose Y chromosomes were cytogeneticallynormal. A total of 16 loci were examined: 15 loci on the longarm between DYS7E and DYZ1, and the YRRM1 locus, a candidategene for the azoospermic factor, AZF. One patient with a perlcentricinversion of the Y chromosome was also included. We detectedmicro-deletions in ten individuals. The YRRM1 gene was Involvedin only three of them. The remaining seven patients showed deletionbetween DYS7C and DYS239 in common, indicating the presenceof at least one additional gene, deletion of which causes azoospermia. 相似文献
88.
Junya Yoneda Ikuo Saiki Hideji Fujii Fuminori Abe Yutaka Kojima Ichiro Azuma 《Clinical & experimental metastasis》1992,10(1):49-59
We have investigated the effect of the immunomodulator ubenimex (hereafter referred to as bestatin) on the enzymatic degradation
of the extracellular matrix by human renal cell carcinoma SN12M cells during the invasive process. The invasion of SN12M cells
into reconstituted basement membrane (Matrigel) was inhibited by the presence of bestatin in a concentration-dependent manner.
However, bestatin did not have any effect on tumor cell adhesion and migration to the extracellular matrices which may be
involved in tumor cell invasion. Bestatin inhibited the degradation of type IV collagen by tumor cells, but not by tumor-conditioned
medium (TCM), in a concentration-dependent manner. We also found that bestatin inhibited hydrolysing activities towards substrates
of aminopeptidases in SN12M cells. Since bestatin was found to inhibit aminopeptidase activity, the inhibition of tumor invasion
by bestatin is likely to be associated with its action as an enzyme inhibitor. Bestatin only slightly inhibited tumor cell
plasmin activity, which can lead to the conversion of the latent collagenase to the active form, but this slight effect was
not significant. The zymography of TCM from SN12M cells showed that the treatment of tumor cells with bestatin resulted in
the disappearance of the 68 kDa type IV collagenase-enzyme level (active form) and slight reduction of the 72 kDa type IV
collagenase-enzyme level (latent form). These results indicated that bestatin may inhibit tumor cell invasion through a mechanism
involving its inhibitory action on aminopeptidases in tumor cells, suggesting that the aminopeptidase may partly be associated
with the conversion of a latent form of type IV procollagenase to an active form or the secretion of the collagenases from
tumor cells. 相似文献
89.
Hidekazu Shigematsu Yutaka Kobayashi Sumio Tateno Yoshiyuki Hiki Sadahito Kuwao 《Pathology international》1992,42(2):91-98
The correlation between glomerular immune deposition and histological alteration was studied in serial paraffin sections of kidney biopsy specimens from patients with IgA nephritis using the peroxidase antiperoxidase (PAP) method. IgA deposition was detected in preserved glomerular extracellular matrix or in newly formed interposed matrix along the capillary loops (mesangial interposition). On the other hand, deposition of IgA was scanty or absent in the distorted extracellular matrix where severe exudative inflammation or extracapillary escape of exudates was occurring segmentally. Ultrastructurally such extracellular matricial destruction was expressed as splitting or thinning of the lamina densa, as well as mesangial edema, reticulation or mesangiolysis of the axial matrix. Therefore it appears that at least two types of change in the extracellular matrix are induced by IgA immune complexes in IgA nephritis; immunogenic deposition and destruction. 相似文献
90.
Kono Y Horie M Takano M Otani H Xie LH Akao M Tsuji K Sasayama S 《Pflügers Archiv : European journal of physiology》2000,440(5):692-698
Functional ATP-sensitive K (KATP) channels have an octameric subunit structure with four pore-forming subunits (Kir6.x) and four sulfonylurea receptors (SURx). In the present study, the properties of the heteromeric KATP channel whose pore subunits are composed of Kir6.1 and Kir6.2 were examined using a heterologous expression system. In COS7 cells co-transfected with Kir6.1, Kir6.2 and SUR2A at a ratio of 1:1:2, KATP channels showed various unitary conductances between those of Kir6.1/SUR2A (33.6+/-4.2 pS) and Kir6.2/ SUR2A (67.1+/-1.6 pS). Kir6.1-6.2 tandem protein, constructed by fusing the C-terminus of Kir6.1 to the N-terminus of Kir6.2 with a ten glutamine linker sequence, also formed a channel with an intermediate conductance (58.9+/-1.5 pS). Kir6.2 and Kir6.1-6.2 showed similar sensitivity to ATP4-: half-maximal inhibition (IC50) was obtained at 14.1+/-12.8 microM and 17.6+/-9.6 microM, respectively. In the presence of Mg2+, Kir6. 1-6.2 was significantly less sensitive than Kir6.2 to MgATP (IC50=95.5+/-49.6 microM versus 18.9+/-5.0 microM). These results suggest that Kir6.1 and Kir6.2 are endowed with the potential to form a heteromeric KATP channel, which has a low sensitivity to MgATP. 相似文献