全文获取类型
收费全文 | 6587篇 |
免费 | 453篇 |
国内免费 | 31篇 |
专业分类
耳鼻咽喉 | 42篇 |
儿科学 | 173篇 |
妇产科学 | 79篇 |
基础医学 | 848篇 |
口腔科学 | 131篇 |
临床医学 | 504篇 |
内科学 | 1633篇 |
皮肤病学 | 231篇 |
神经病学 | 682篇 |
特种医学 | 231篇 |
外科学 | 940篇 |
综合类 | 34篇 |
预防医学 | 223篇 |
眼科学 | 146篇 |
药学 | 500篇 |
中国医学 | 15篇 |
肿瘤学 | 659篇 |
出版年
2024年 | 15篇 |
2023年 | 162篇 |
2022年 | 298篇 |
2021年 | 527篇 |
2020年 | 223篇 |
2019年 | 283篇 |
2018年 | 318篇 |
2017年 | 237篇 |
2016年 | 247篇 |
2015年 | 255篇 |
2014年 | 286篇 |
2013年 | 325篇 |
2012年 | 516篇 |
2011年 | 505篇 |
2010年 | 273篇 |
2009年 | 209篇 |
2008年 | 342篇 |
2007年 | 304篇 |
2006年 | 315篇 |
2005年 | 279篇 |
2004年 | 276篇 |
2003年 | 217篇 |
2002年 | 180篇 |
2001年 | 74篇 |
2000年 | 58篇 |
1999年 | 39篇 |
1998年 | 34篇 |
1997年 | 31篇 |
1996年 | 18篇 |
1995年 | 14篇 |
1994年 | 17篇 |
1993年 | 18篇 |
1992年 | 28篇 |
1991年 | 14篇 |
1990年 | 26篇 |
1989年 | 15篇 |
1988年 | 11篇 |
1987年 | 9篇 |
1986年 | 6篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1981年 | 6篇 |
1977年 | 5篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1972年 | 4篇 |
1968年 | 4篇 |
1967年 | 3篇 |
排序方式: 共有7071条查询结果,搜索用时 15 毫秒
71.
Yuki Ono Gouji Toyokawa Tetsuzo Tagawa Kayo Ijichi Yoshinao Oda Masaki Mori 《Annals of thoracic and cardiovascular surgery》2022,28(3):223
Immunoglobulin G4-related disease (IgG4-RD) is a fibroinflammatory condition which involves various organs. This is a very rare case of IgG4-related lung disease (IgG4-RLD) with the invasion into diaphragm. The patient was a 71-year-old man with a long-term exposure to asbestos who had a mass shadow in the left lower lung lobe, which was suspected to invade the left diaphragm on computed tomography (CT). Positron emission tomography (PET)/CT also presented an avid intake of fluorodeoxyglucose in the mass, which suspected lung cancer. Although bronchoscopic biopsy could not lead to the definite diagnosis, we performed left lower lobectomy combined with the resection of left diaphragm. The specimen showed the features of IgG4-RLD on pathology: the vein stenosis and fibrosis around the vein, the infiltration of IgG4-positive cells, and IgG cells to IgG4 cells ratio of 40%. Furthermore, there were inflammatory cells infiltrating to the diaphragm. 相似文献
72.
Tomoki Kuge Kiyoharu Fukushima Yuki Matsumoto Haruko Saito Yuko Abe Eri Akiba Kako Haduki Tadayoshi Nitta Akira Kawano Michio Tanaka Yumi Hattori Takahiro Kawasaki Takanori Matsuki Takayuki Shiroyama Daisuke Motooka Kazuyuki Tsujino Keisuke Miki Masahide Mori Seigo Kitada Shota Nakamura Tetsuya Iida Atsushi Kumanogoh Hiroshi Kida 《Emerging infectious diseases》2022,28(7):1437
Unidentified Mycobacterium species are sometimes detected in respiratory specimens. We identified a novel Tsukamurella species (Tsukamurella sp. TY48, RIMD 2001001, CIP 111916T), Tsukamurella toyonakaense, from a patient given a misdiagnosis of nontuberculous mycobacterial pulmonary disease caused by unidentified mycobacteria. Genomic identification of this Tsukamurella species helped clarify its clinical characteristics and epidemiology. 相似文献
73.
Yuki Yajima Akemi Kosaka Kei Ishibashi Shunsuke Yasuda Hiroki Komatsuda Toshihiro Nagato Kensuke Oikawa Masahiro Kitada Masanori Takekawa Takumi Kumai Kenzo Ohara Takayuki Ohkuri Hiroya Kobayashi 《Cancer science》2022,113(8):2526
Although neoantigens are one of the most favorable targets in cancer immunotherapy, it is less versatile and costly to apply neoantigen‐derived cancer vaccines to patients due to individual variation. It is, therefore, important to find highly immunogenic antigens between tumor‐specific or associated antigens that are shared among patients. Considering the cancer immunoediting theory, immunogenic tumor cells cannot survive in the early phase of tumor progression including two processes: elimination and equilibrium. We hypothesized that highly immunogenic molecules are allowed to be expressed in tumor cells after an immune suppressive tumor microenvironment was established, if these molecules contribute to tumor survival. In the current study, we focused on TWIST1 as a candidate for highly immunogenic antigens because it is upregulated in tumor cells under hypoxia and promotes tumor metastasis, which is observed in the late phase of tumor progression. We demonstrated that TWIST1 had an immunogenic peptide sequence TWIST1140–162, which effectively activated TWIST1‐specific CD4+ T‐cells. In a short‐term culture system, we detected more TWIST1‐specific responses in breast cancer patients compared with in healthy donors. Vaccination with the TWIST1 peptide also showed efficient expansion of TWIST1‐reactive HTLs in humanized mice. These findings indicate that TWIST1 is a highly immunogenic shared antigen and a favorable target for cancer immunotherapy. 相似文献
74.
Hayashi Yuki Wagatsuma Kohei Nojima Masanori Yamakawa Tsukasa Ichimiya Tadashi Yokoyama Yoshihiro Kazama Tomoe Hirayama Daisuke Nakase Hiroshi 《Journal of gastroenterology》2021,56(5):421-421
Journal of Gastroenterology - A correction to this paper has been published: https://doi.org/10.1007/s00535-021-01786-z 相似文献
75.
Yosuke Kunishi Megumi Tsukamoto Takafumi Yanagibashi Yuki Sodani Kazuhiro Niwa Koichiro Yoshie Yoshio Kato Shin Maeda 《Internal medicine (Tokyo, Japan)》2021,60(6):851
Cecal volvulus is an uncommon cause of colonic obstruction. First-line treatment for cecal volvulus is surgery, as nonoperative management is rarely achievable. We herein report an extremely rare case of a patient with spontaneously resolved cecal volvulus; no recurrence occurred without elective surgery. A 47-year-old woman presented with acute lower abdominal pain. She was misdiagnosed with small bowel obstruction and treated conservatively. A few hours later, she was correctly diagnosed with cecal volvulus. Subsequently, her symptoms and computed tomography findings of cecal volvulus completely disappeared. She refused elective surgery, but no recurrence occurred during five months of follow-up. 相似文献
76.
77.
78.
Yuki Katayama Hiroko Murakami-Kuroda Longzhu Cui Keiichi Hiramatsu 《Antimicrobial agents and chemotherapy》2009,53(8):3190-3196
Vancomycin (VAN)-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA) isolates are considered to have emerged from VAN-susceptible S. aureus (VSSA) by spontaneous mutation during VAN exposure. We previously reported that laboratory mutant H14, obtained from VSSA strain ΔIP by exposure to imipenem (IPM), showed overexpression of the vraSR two-component system and a typical hVISA phenotype. In the present study, to elucidate the mechanism of VSSA conversion to hVISA, we further characterized strain H14 by determining its whole-genome sequence, morphology, cell wall synthetic activity, and gene expression. Genome sequencing revealed that H14 harbored a mutated vraS (designated vraSH14) that caused an amino acid substitution (S329→L). This mutation is different from the VraS mutation (N5→I) identified in representative clinical hVISA strain Mu3. However, H14 exhibited a phenotype similar to that of Mu3, including heterogeneous resistance to VAN, enhanced cell wall synthetic activity, and vraSR overexpression. Replacement of the vraS gene of ΔIP with the mutated vraSH14 gene confirmed that the S329→L substitution was responsible for both the upregulation of vraSR and conversion to the hVISA phenotype. This conversion was also achieved by using the vraS gene of Mu3, which carries a mutation (N5→I), but not with the native vraS gene of strain N315. Finally, we carried out a study to analyze the appearance of hVISA from VSSA by exposure of ΔIP to selective concentrations of VAN and beta-lactam antibiotics. A total of 8 and 5 hVISA isolates were detected among 50 isolates selected with VAN and IPM, respectively. Among the 13 hVISA mutants, mutation in vraSR was detected only in mutant strain H14, suggesting that additional mutational mechanisms can be responsible for evolution to the hVISA phenotype. We conclude that exposure not only to VAN but also to beta-lactams may select for reduced glycopeptide susceptibility in S. aureus.Methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) is a major cause of serious nosocomial infections, and the emergence of virulent MRSA strains in the community is of particular concern (6). Vancomycin (VAN) still serves as the main therapeutic agent for infections caused by multiresistant MRSA strains (17). However, MRSA strains with various degrees of reduced susceptibility to glycopeptides, vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA) strains, have emerged among multidrug-resistant MRSA clinical strains (9, 16).Recently, we identified several genes that are overexpressed in VISA strain Mu50 and hVISA Mu3 compared to their levels of expression in their isogenic VAN-susceptible S. aureus (VSSA) strain, strain Mu50Ω (13); and among these, we found the overexpression of the vraSR two-component system (TCS), an upregulator of the S. aureus cell wall biosynthesis pathway (12, 13). We also demonstrated that the vraS gene is overexpressed in ΔIP-H14 (H14), a laboratory-derived hVISA strain obtained by selecting VSSA strain N315ΔIP (ΔIP) with 8 mg/liter of imipenem (IPM) (12) and showed that a single amino acid substitution in VraS was present in H14, Mu3, and Mu50 (10a).In the study described here, we further characterized hVISA strain H14, investigated the role of the vraS mutation on the phenotype of H14, and evaluated the rates of selection of hVISA from VSSA ΔIP following exposure to VAN and beta-lactam antibiotics. 相似文献
79.
Kakiuchi Y Yuki N Iyoda K Sugiyasu Y Kaneko A Kato M 《Journal of gastroenterology》2004,39(12):1189-1195
Background The clinical relevance of the circulating soluble form of the Fas-Receptor (sFas) was investigated in patients with hepatitis C receiving type 1 interferon (IFN) therapy.Methods sFas was quantified by enzyme-linked immunosorbent assay in 66 hepatitis C virus (HCV) carriers and 30 HCV-naive or previously infected controls. The levels were then monitored during enhanced treatment with type 1 IFNs in 15 chronic hepatitis C patients.Results The HCV carriers had high levels of sFas compared with controls (3.8 ± 1.3 vs 2.7 ± 0.8ng/ml; P < 0.001). sFas levels in patients with chronic HCV infection were directly related to serum alanine aminotransferase levels (r = 0.440; P < 0.001) and the histological grade (r = 0.403; P = 0.019). Among necroinflammatory reactions, only piecemeal necrosis showed a correlation with sFas levels (r = 0.556; P = 0.001). Pretreatment sFas levels, however, were not predictive of therapeutic outcomes. A sustained virological response to enhanced IFN therapy showed a relation to only the pretreatment HCV load. Interestingly, circulating sFas was upregulated when IFN- was administered at short intervals (3MU/every 12h). This upregulation was accompanied by parallel aminotransferase elevation, which was observed regardless of a virological response.Conclusions sFas elevation, in parallel with the severity of liver injury, suggests the possible upregulation of hepatic Fas expression and the Fas-mediated pathway in both HCV- and type 1 IFN-induced liver injury. The essential function of sFas to protect hepatocytes against Fas-mediated liver injury was not evident in these clinical settings. 相似文献
80.
Kim EH Ikegame K Kawakami M Nishida S Fujioka T Taniguchi Y Masuda T Oka Y Kawase I Ogawa H 《International journal of hematology》2004,80(5):449-452
We present the case of a patient with myelodysplastic syndrome who experienced leukemia transformation and subsequently underwent transplantation of unmanipulated peripheral blood stem cells from a haploidentical sibling mismatched at 3 HLA antigens, along with a reduced-intensity regimen (fludarabine, busulfan, and anti-T-lymphocyte globulin) and tacrolimus-containing graft-versus-host disease (GVHD) prophylaxis. The patient experienced graft rejection but successfully underwent a second transplantation from the same donor with a slightly intensified conditioning regimen. Although the patient developed life-threatening cytomegalovirus (CMV) pneumonia following the second transplantation, he recovered completely from the pneumonia with intensive supportive therapy. He is still in complete remission past day 1000 in the absence of GVHD. As far as we know, this report is the first to describe a successful second transplantation that was performed for graft rejection following HLA-haploidentical nonmyeloablative stem cell transplantation. Furthermore, we emphasize that patients should be carefully monitored for CMV infection when reduced-intensity conditioning is given repeatedly over a short period. 相似文献