宫颈癌细胞增殖和DNA倍体与放射敏感性的关系

为研究宫颈癌细胞增殖参数 S期比例 (SPF)、增殖指数 (PI)和 DNA倍体与宫颈癌放射敏感性的关系 ,探讨它们在预测宫颈癌放射敏感性中的价值。对 4 7例宫颈癌患者放疗前行宫颈癌组织取材 ,进行流式细胞术分析 ,在放疗过程中每周测量 1次宫颈局部瘤床的大小 ,计算肿瘤消退 1/ 2所需照射剂量 (D0 .5 ) ,研究 SPF、PI和 DNA倍体与D0 .5之间的关系。结果显示 :SPF与 D0 .5呈正相关 (r=0 .6 0 4 ,P<0 .0 1) ,PI与 D0 .5无明显关系 ,DNA倍体对 D0 .5的影响有显著性意义 (P<0 .0 5 )。提示 :宫颈癌细胞放疗前 SPF和 DNA倍体与放射敏感性有关 ,该两项指标有可能成为宫颈癌放射敏感性的预测指标     

Sympathoadrenal excitation and inhibition by lower brainstem stimulation in cats

Effects of stimulation of brainstem sites on hemodynamics and plasma catecholamine levels were assessed in cats under chloralose-urethane anesthesia. Pressor areas of the dorsal medulla (DM) and ventrolateral medulla (VLM) and the depressor area of the paramedian reticular nucleus (PRN) were stimulated electrically using a monopolar electrode, or chemically using sodium glutamate microinjection. Plasma levels of norepinephrine (NE) and epinephrine (EPI) were measured in caval blood above the adrenal veins. Electrical stimulation of the DM and VLM produced increases in blood pressure and in plasma NE and EPI levels that were enhanced after acute vagotomies. The NE and EPI responses were attenuated after acute, bilateral adrenalectomies, confirming augmented adrenomedullary secretion, whereas the pressor responses were intact. Injection of sodium glutamate into the same pressor regions of the DM or VLM also produced pressor responses and elevated plasma catecholamine levels, indicating that the responses resulted from activation of neuronal perikarya. Stimulation of the PRN attenuated pressor and catecholamine responses during stimulation of the DM and VLM. The results indicate that pressor responses during stimulation of the DM and VLM are due at least partly to activation of perikarya in these regions, are associated with but not dependent on adrenomedullary activation, and are enhanced after vagotomy; and that neurons of the PRN exert inhibitory modulation of the pressor and adrenomedullary responses during stimulation of VLM and DM.     

端粒酶突变体对肝癌细胞端粒酶活性的作用

目的　观察端粒酶突变体对肝癌细胞端粒酶活性和生长抑制的作用。方法　利用质脂体转染法在培养的肝癌细胞中导入端粒酶突变体，并与维甲酸和人参皂甙对照，利用TRAP－银染方法对不同时期肝癌细胞进行了端粒酶活性的测定，并观察各期细胞生长情况。结果　在加入端粒酶突变体后肝癌细胞端粒酶活性明显降低，细胞出现明显凋亡现象，其作用效果与维甲酸基本相同。结论　端粒酶突变体对肝癌细胞的抑制作用可能是通过抑制端粒酶活性途径实现的。     

Post-stimulus depression of reflex changes in circular muscle activity in the guinea pig small intestine.

The extent and time course of depression of successive reflex responses recorded with intracellular microelectrodes from the circular smooth muscle of the guinea pig small intestine were determined. Two stimuli were used, distension and distortion of the mucosa by compression; these were applied either at the same or at different sites. Excitatory responses oral and inhibitory responses anal to the stimuli were recorded. Post-stimulus depression of both ascending excitatory and descending inhibitory reflexes occurred, but the extent of depression was slightly less for the descending inhibition. A conditioning distension lasting 9 s depressed the excitatory response to a test distension applied 2 s later at the same site by 90%. After 30 s the depression was 50% and test responses were normal if inter-stimulus intervals were increased to 2 min. Increasing the duration of the conditioning stimulus increased the depression. Post-stimulus depression was less for compression stimuli than for distension stimuli and prior mucosal compression had almost no effect on responses to subsequent distension. The post-stimulus depression was greater if conditioning and test stimuli were at the same rather than different sites. For different sites, conditioning stimuli at 15 mm from the recording site (near) depressed responses to stimuli at 30 mm (far) to a greater extent than far stimuli depressed responses to near stimuli. If the conditioning stimulus at 15 mm was maintained until after the far test stimulus was applied, depression of the test response did not occur. It is concluded that the major sites of post-stimulus depression are at the synapses between primary sensory neurons and the first interneurons of reflex pathways, and that post-stimulus depression also occurs at other places in the pathway, presumably at synapses between interneurons or between interneurons and motor neurons.     

Comparative Study on the Immunogenicity between Recombinant MS-Sj26GST Vaccine and Recombinant BCG-Sj26GST Vaccine in Schistosoma japonicum

TuberculosisandSchistosomiasisarethemajorcontagiousdiseaseswhicharethemostdangeroustothepeople’shealth Inordertogetridofthem ,wemustlookforamoreusefulvaccine Bythetech niquesofmolecularbiology ,2 6 0 0 0DaGlutathionStransferase (GST) genewasclonedintotheE coli MycobacteriumtransferringandexpressionvectorpBCG 2 0 0 0totransformittoMycobacteriumsmeg matismc2 15 5 (MS)andBCGseparatelyinordertoconstructrMS Sj2 6GSTvaccineandrBCG Sj2 6GSTvaccine Inthisstudy ,theBALB/cmicewereimmu niz…     

尼莫地平复方丹参注射液治疗新生儿缺氧缺血性脑病疗效观察

目的观察尼莫地平联合复方丹参注射液治疗新生儿缺氧缺血性脑病(HIE)的疗效.方法 128例HIE患儿随机分为复方丹参注射液组32例,尼莫地平组,尼莫地平加复方丹参注射液,与对照组.结果复方丹参注射液组、尼莫地平组、尼莫地平加复方丹参注射液治疗组总有效率分别为81.3%(26/32)、80.0%(24/30)、97.1%(34/35),对照组总有效率为51.6%(16/31),P＜0.05.结论尼莫地平与复方丹参注射液在治疗HIE时有协同作用,疗效显著,未见副作用发生.     

Soluble Fcγ receptors in periodontal lesions

Soluble Fcγ-binding components were detected in gingival fluid from periodontal lesions by incubation with biotinylated human Fcγ fragments. FcγIII receptor was identified by incubation of gingival fluid with monoclonal antibody. Sodium dodecyl sulfate-polyacrylamide gel electophoresis and Western transfer showed that most of the Fcγ-binding components had minimal mobility in a 4–15% gradient gel under nonreducing conditions. Under reducing conditions, the main band of Fcγ-binding components in gingival fluid migrated corresponding to protein A of 49 kDa. The pattern of Fcγ-binding components was similar in serum and gingival fluid except for the observation in gingival fluid of Fcγ-binding components migrating like standard proteins of 19 to 20 kDa, a size that corresponds to the polypeptide part of FcγII receptor and FcγIII receptor.     

北京市宣武区中学生生殖健康知识调查分析

【目的】掌握青少年性发育特点,以便开展有针对性的青春期生殖健康教育,提高自我保护能力,使其健康的成长。【方法】重点高中2所、普通高中4所、职业高中3所,分别选择同年级、同号班级22个班的1 800名高中生,由专门调查员进班发问卷,要求学生在15 min内完成。学生填写问卷时,采用校方人员回避,问卷不记名方式,填写后当场封存,确保学生的隐私权。【结果】调查学生中生理知识知晓率达到70.74%,性病、艾滋病方面知识知晓率达53.9%,相关避孕知识知晓率15%,学生中的性活动发生率17.9%,缺乏保护意识的学生占25.4%,接触过淫秽品的学生43.5%,对婚前性行为的看法持开放态度的学生占38%。【结论】学生的性观念开放,综合自我保护能力较低,易受疾病和社会危险因素伤害,对其成长过程造成障碍。     

替米沙坦治疗原发性高血压的疗效和安全性

目的 :观察替米沙坦治疗原发性高血压的疗效和安全性。方法 :进行随机、双盲、双模拟、平行分组对照试验。符合条件的轻中度高血压患者随机分为 2组 :替米沙坦组和氯沙坦组。每天一次口服替米沙坦 40mg或氯沙坦 5 0mg ,每 2周随访 1次。 4周后 ,如患者舒张压 (DBP)≥ 90mmHg则增加剂量 ,每天一次口服替米沙坦 80mg或氯沙坦 10 0mg。治疗前后分别查心电图 ,血、尿常规 ,血生化检查和常规查体。 结果 :(1)治疗 8周末 ,替米沙坦组和氯沙坦组患者的DBP均显著降低 ,2组下降幅度相似 (11.9mmHgvs 10 .4mmHg) ;(2 )治疗8周末 ,替米沙坦组收缩压 (SBP)下降幅度稍大于氯沙坦组 (9.5mmHgvs 6.2mmHg) ;(3 )替米沙坦降压有效率为 83 .3 % ,稍高于氯沙坦组 69.2 % ,无统计学差异 ;(4 ) 2组的不良事件发生率相似 (替米沙坦组 3 3 .3 % ,氯沙坦组 3 4.6% )。最常见的不良事件是头晕、头痛 ,多为轻度 ,可迅速缓解。结论 :替米沙坦 40mg～ 80mg ,每天一次口服治疗轻中度高血压安全有效     

Rehabilitation of the patient with chronic back pain. A search for outcome predictors

This prospective study was initiated 3 years ago to evaluate the outcome and to identify predictors of success or failure in patients admitted to a rehabilitation program for chronic low-back pain. Multiple parameters were evaluated, including psychologic data (MMPI, personal interview, pain drawing, etc.), physical measurements (flexibility, strength and endurance), and demographic data concerning the patient's home and working environment. Information was available on each patient admitted to the program prior to his admission, at completion of the program, 6 weeks following completion of the program and 3 months following completion of the program. A telephone interview was carried out 2 1/2 years following the patient's discharge from the program. Linear regression analysis was used to identify the important independent variables with regard to the dependent variables of relief of back pain, return to work and increased activities at home. Demographic data were of no value as a predictor with the exception of age and returning to work. The patients over the age of 50 returned to work with much less frequency than those less than 50. Psychologic information from the MMPI and similar tests were of no value. The personal preadmission interview of a trained psychologist, however, was a good predictor of an individual's eventual return to work and overall improvement. Worker's Compensation and other litigation was a negative factor in a patient's prognosis. The treatment team's prognosis at the time of discharge from the program was the best overall predictor of a patient's chance of success or failure in the longterm.