FANCD2、PALB2表达水平与非小细胞肺癌临床特征及预后的关系

目的 探讨范科尼贫血D2蛋白（FANCD2）、乳腺癌易感基因2定位协作蛋白（PALB2）表达水平与非小细胞肺癌（NSCLC）临床特征及预后的关系。方法 选取2017年11月—2019年10月成都市第二人民医院收治的194例NSCLC患者作为研究对象。将手术过程中取得癌组织标本作为NSCLC组,将对应的癌旁组织标本作为癌旁组,每组194例。采用免疫组织化学法检测FANCD2、PALB2在NSCLC患者癌组织及癌旁组织中的表达情况;分析FANCD2、PALB2表达与NSCLC患者临床病理特征的关系;采用Kaplan-Meier法绘制生存曲线,采用Cox比例风险模型分析探讨NSCLC患者预后的影响因素。结果 NSCLC组FANCD2、PALB2阳性率高于癌旁组（P <0.05）。Spearman相关性分析显示,NSCLC患者癌组织中FANCD2蛋白与PALB2蛋白呈正相关（r_s =0.486,P <0.05）。不同年龄、性别、吸烟、组织学分型、肿瘤直径患者FANCD2、PALB2阳性率比较,差异均无统计学意义（P >0.05）。TNM分期为Ⅲ、Ⅳ期,低分化,有淋巴结转移患者高于TNM分期为I、Ⅱ期,中/高分化,无淋巴结转移患者（P <0.05）。多因素逐步Cox回归分析结果显示：TNM分期Ⅲ、Ⅳ期［H^R=4.125,（95% CI：2.187,10.035）］、低分化［H^R=3.146,（95% CI：3.115,9.264）］、淋巴结转移［H^R=4.124,（95% CI：3.005,13.145）］、FANCD2阳性［H^R=5.146,（95% CI：3.784,12.689）］、PALB2阳性［H^R=4.563,（95% CI：2.845,7.398）］是NSCLC患者复发的影响因素（P <0.05）。多因素逐步Cox回归分析结果显示,TNM分期Ⅲ/Ⅳ期［H^R=3.689,（95% CI：2.963,11.254）］、低分化［H^R=2.167,（95% CI：1.998,5.996）］、淋巴结转移［H^R=5.648,（95% CI：3.552,12.953）］、FANCD2阳性［H^R=3.886,（95% CI：2.958,12.775）］、PALB2阳性［H^R=4.633,（95% CI：1.968,11.547）］是NSCLC患者预后的影响因素（P <0.05）。FANCD2阳性患者与阴性患者生存率比较,差异有统计学意义（P <0.05）;PALB2阳性患者与阴性患者的生存率比较,差异有统计学意义（P <0.05）。结论 FANCD2、PALB2在NSCLC患者癌组织中呈高表达,与TNM分期、分化程度、淋巴结转移密切相关,可作为辅助评估患者预后和复发的潜在标志物。     

腰椎间盘突出症与腰椎间盘突出症合并阻塞性睡眠呼吸暂停患者的对比研究

目的 探究腰椎间盘突出症（LDH）与阻塞性睡眠呼吸暂停（OSA）之间的关联,为LDH的临床诊治策略及治疗方案提供参考依据。方法 选取2018年1月—2020年3月中国医科大学附属第一医院骨科行外科手术治疗的485例LDH患者,按是否合并OSA进行分组研究。结果 485例LDH患者中合并OSA的307例,发生率为63.3%。LDH合并OSA组主观症状、临床症状、日常活动受限程度日本骨科协会（JOA）腰痛评分低于单纯LDH组（P <0.05）;两组膀胱功能JOA评分比较,差异无统计学意义（P> 0.05）;LDH合并OSA组视觉模拟评分法（VAS）评分高于单纯LDH组（P <0.05）。Pfirrmann 5级评分法中4、5级例数构成比LDH合并OSA组较单纯LDH组高（P <0.05）。两组患者的硬膜撕裂率、神经受损率、血肿率、切口感染率等并发症发生率比较,差异无统计学意义（P>0.05）。单纯LDH组与LDH合并OSA组术后3、6、12和24个月静息状态下JOA和VAS评分比较,采用重复测量设计的方差分析,结果：(1)不同时间点的JOA和VAS评分有差异（...     

金属表面TiO2薄膜的溶胶-凝胶法制备及其血液相容性研究

通过溶胶 凝胶法制备TiO2 薄膜对 316L不锈钢和NiTi形状记忆合金进行表面改性处理。研究发现 ,经 5 0 0℃处理 1h的薄膜结构致密 ,膜层均匀平滑 ,薄膜主要由锐钛矿相TiO2 构成 ,随热处理温度的提高 ,锐钛矿相逐渐转变为金红石相。电化学腐蚀和动态凝血时间及溶血率测试表明 ,通过溶胶 凝胶法制备TiO2 膜进行表面改性的 316L不锈钢和NiTi合金的抗模拟体液腐蚀性提高 ,动态凝血时间延长 ,溶血率下降 ,说明溶胶 凝胶法制备TiO2 膜可以提高金属植入物的血液相容性     

Morphologic and immunologic evidence of composite B- and T-cell lymphomas. A report of three cases developing in follicular center cell lymphomas

Composite lymphoma (CL) may be defined as two lymphomas, differing as to their cell of origin, that occur simultaneously in the same tissue specimen. While CL usually is indicated histopathologically by at least two morphologically distinct lymphomatous proliferations, the proof that these proliferations are separate and distinct neoplasms requires immunologic analysis. Many so-called cases of CL actually represent the well-known phenomenon of lymphoid transformation, in which there is a small cell and a large cell component in the same specimen. Immunologic studies in these cases have shown that the cytologically distinct neoplastic cells represent different stages in the same cell line. While studying a large series of follicular center cell (FCC) lymphomas, the authors recognized three cases in which there was both morphologic and immunologic evidence of a true CL. Following an initial diagnosis of a nodular FCC lymphoma, rebiopsies from 21 to 62 months later showed the coexistence of a nodular FCC (B-cell) component and a diffuse large cell (T-cell) component.     

CCL25和CCL28及其相应受体CCR9和CCR10

CCL25和CCL28是CC趋化因子家族成员,主要表达于胸腺树突状细胞和黏膜上皮细胞,CCL25和CCL28相应的受体分别是CCR9和CCR10,表达于T淋巴细胞和B淋巴细胞。CCL25和CCL28及其相应受体对循环IgA浆母细胞和T淋巴细胞的归巢起重要作用,而且CCL28还具有广谱的抗微生物活性。     

An accessory open reading frame (orf-x) of jaagsiekte sheep retrovirus is conserved between different virus isolates

Jaagsiekte sheep retrovirus (JSRV) is the etiological agent of a contagious lung tumour of sheep known as sheep pulmonary adenomatosis (syn: ovine pulmonary carcinoma, jaagsiekte). JSRV exhibits a simple genetic organization, characteristic of the type D and type B retroviruses, with the canonical retroviral sequences gag, pro, pol and env encoding the structural proteins of the virion. An additional open reading frame (orf-x), of approximately 500 bp overlapping pol, is present in the only two complete sequences of JSRV published to date. Since very little information is available on the biology of JSRV it is important to establish if orf-x is conserved between different virus isolates. In this study we analysed the orf-x region of JSRV isolates collected from the United Kingdom, Italy, Spain and South Africa. In addition we also analysed the presence of orf-x in JSRV-related endogenous sequences (enJSRVs) present in the sheep genome. Orf-x was highly conserved in all the exogenous isolates (n=10) and in most of the endogenous sequences (n=8). Thus orf-x may be an accessory gene of JSRV and haves a biological function which might be advantageous to JSRV. Phenetic analysis conducted on the complete orf-x nucleotide sequences seems to highlight the presence of three distinct groups statistically well supported by bootstrapping: i) exogenous JSRV sequence from the UK; ii) exogenous JSRV sequences from Southern Europe and iii) the exogenous South African strain plus all the endogenous sequences analyzed and collected from Australia, Italy, UK and South Africa.     

The neurofibromatosis gene in human pituitary adenomas

The neurofibromatosis 1 (NF1) gene encodes a protein neurofibromin, which contains a glutamyl transpeptidase (GTP)-activating protein (GAP)-related domain: NF1 GRD. This domain is able to down-regulate P^21ras by stimulating its intrinsic GTPase. Because P^2lras has an important role in regulating growth and differentiation, somatic mutations in the NF1 gene may result in mutant neurofibromins that might interfere with the Ras signaling pathway and contribute to the development of tumors. In this study, we used polymerase chain reaction (PCR)-coupled single-stranded conformational polymorphism (SSCP) and DNA sequencing to examine possible mutations in the NF1 GRD in human pituitary tumors. We screened 36 nonfunctioning and 20 growth hormone—secreting adenomas. No mutation was detected in these tumors. Our results indicate that inactivation of neurofibromin may not have a primary role in the formation of pituitary adenomas.