Partial characterization of a non-proteinaceous, low molecular weight antigen of Eimeria tenella

A low molecular weight (LMW) antigen of Eimeria tenella, initially identified using a murine monoclonal antibody (mAb C₃4F₁) raised against E. tenella sporozoites, was partially characterized using enzymatic degradation, solvent extraction, and immunization into various inbred lines of mice. The LMW antigen could be isolated using Folch extraction (methanol/chloroform/water) and the epitope recognized by mAb C₃4F₁ was resistant to degradation by α-amylase, pronase, and proteinase K, but was sensitive to sodium m-periodate treatment or digestion using mixed glycosidases (from Turbo cornutus). These observations suggest that the antigenic epitope recognized by mAb C₃4F₁ is carbohydrate-dependent and, based on our ability to isolate the LMW antigen by Folch extraction, the epitope probably resides on a polar glycolipid. The inability of sporozoite-immunized nude mice to elicit a serum antibody response to this molecule indicates that it acts as a T-dependent antigen. Furthermore, sporozoite-immunized male CBA/N mice (with an X-linked immunodeficiency) also failed to elicit a serum antibody response to this molecule, which is consistent with a carbohydrate antigenic epitope. We propose that this antigenic molecule be designated ET-GL1 to reflect its origin and probable structure (E. tenella glycolipid 1). Received: 30 June 1999 / Accepted: 2 December 1999     

Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus

The open reading frame 3 (ORF3) of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted 154-amino acid protein, which lacks similarities to any known protein, and is named 3b. In this study, it was shown that 3b protein was predominately localized to nucleus with EGFP tag at its N- or C-terminus. The localization patterns were similar in different transfected cells. Immuno-fluorescence assay revealed that 3b protein was co-localized well with C23 in nucleolus. C23, B23 and fibrillarin all are important nucleolar proteins, which localize in the region of the nucleolus. Co-transfection of p3b-EGFP with pC23-DsRed, pB23-DsRed and pfibrillarin-DsRed further confirmed 3b's nucleolus localization. With construction of serial truncated mutants of 3b, a region (residues 134-154 aa) responsible for nucleolar localization was determinated in 3b protein. These results provide a new insight for further functional studies of SARS-CoV 3b protein.     

Genetic alterations in primary and secondary hyperparathyroidism

Hyperparathyroidism refers to a term representing a wide spectrum of parathyroid disorders that are characterized by the increased production of parathyroid hormone. Hyperparathyroidism was once thought to be tare but is now more commonly recognized, aifecting 1 in 500 women over 40 years of age. Yet the interpretation of parathyroid pathology is still controversial and confusing. Over the past 10 years, genetic changes ( ret and menin genes) involved in the pathogenesis of MEN 2 and MEN 1 have been discovered in succession. Different mutations of the calcium-sensing receptor gene have been identified in neonatal severe hyperparathyroidism and familial hypocalciuric hypercal-cemia, respectively. The HRPT 2 gene responsible for the development of heredltaty hyperparathyroidism and jaw tumors has been localized on the 1q21–31 locus. Several genetic alterations have also been characterized in primary and secondary hyperparathyroidism. Different genetic alterations appear to involve the development of different types of hyperparathyroidism. These novel advances give us new insights into the pathogenesis of hyperparathyroidism and allow better differentiation between the different types of parathyroid disorders.     

重点高中生的心理状态与高考成绩的相关性研究

目的;了解重点高中学生的心理状态与高考成绩之间的关系。方法：以398例应届生为对象,应用16PF人格测验,气质测量工具（STI,TTI）及焦虑自评量表（SAS）,对考生进行测试分析,结果：文理科学生的16PF的人格特质有明显的差别,判别分析和多因素回归分析显示16PF人格特质中的Q1因素（实验性F＝8．76,P＜0．01）,Q2因素（独立性F＝8．23,P＜0．01）,与高考成绩呈正相关,与M因素（幻想性F＝4．88,P＜0．01）,呈负相关;气质中的神经过程灵活性（F＝8．15,P＜0．01）和时间特质中的灵活性（F＝12．36,P＜0．01）与高才成绩呈正相关。结论：高考生的实验性,独立性高分及幻想性低分的个性特质和气质上的灵活性有助于高考成绩的发挥,学生文理科分班宜参考学生人格特质这一特点,。     

眼科设备数据库设计初探

本文介绍了一所眼科医院设备数据库的设计思路,数据库内容以及用途。     

严重颅眶损伤的特点和救治

目的探讨严重颅眶损伤的特点和救治。方法回顾分析1990年1月～1997年12月收治的32例严重颅眶损伤患者的临床资料。结果32例严重颅眶损伤的临床特点为：脑损伤局限．意识障碍较轻,嗅神经、视审经损伤多见,脑脊液漏发生率高,颅眶畸形严重。结论应重视院前急救,尽早转送专科医院．除清除颅内血肿外,应注重视神经损伤、脑脊液漏和颅眶畸形手术处理．积极治疗并发症,有利于视神经功能的恢复,并能有效预防脑脊液漏引起的颅内感染．改善面部外观。     

Malignant tumors of the nasopharynx

One hundred and twenty patients with malignancy of the nasopharynx were reviewed. The most common presenting symptoms of this disease were cervical mass and hearing loss. Neoplasm occurred three times more frequently in males than in females. Cranial nerve dysfunction was present in 17.5% of patients at the time of initial evaluation. The overall 3- and 5-year determinate survival for the entire series of nasopharyngeal malignancies was 38.9, and 27.0%, respectively. Nonsquamous cell malignancies demonstrated a better 5-year survival (42%) than squamous cell and undifferentiated neoplasms (24%). The presence of cervical metastases offered a poorer prognosis. A 39.6% 5-year survival was recorded for patients without nodal disease. Massive lymphadenopathy or bilateral cervical metastases forecasted an ominous 5-year survival of 13.6%. Control rate was greatest among patients with tumors restricted to one area of the nasopharynx and without regional metastases. The 5-year survival of such patients was 71.4% compared to 13.3% for patients having tumor extension beyond the nasopharynx producing cranial nerve impairment or bone erosion.     

中西医结合治疗急性脑梗塞疗效观察

根据中医血水相关理论 ,拟定活血利水煎为基本方 ,配用西药以防严重并发症 ,治疗急性脑梗塞 85例 ,并设单纯西药治疗组 6 0例作对照。结果表明 ,治疗组的临床疗效较对照组有非常显著性差异 (P <0 0 1) ,中药活血利水煎具有改善血液流变性 ,降高血压、高血糖、高血脂的作用。活血利水为脑梗塞急性期治疗大法     

帕司烟肼介入凝胶体外抗结核活性及安全性研究

目的 观察帕司烟肼凝胶体外抗结核作用和支气管介入的安全性。方法 手工法、仪器法分别测定帕司烟肼及其凝胶的最小抑菌浓度和最小杀菌浓度及家兔经支气管介入的安全性试验。结果 帕司烟肼凝胶对H37Rv标准株、牛型结核分枝杆菌、草分枝杆菌MIC值分别为0．1、0，1、0．4mg／L，MBC值分别为0，2、0．2和1．6mg／L；帕司烟肼凝胶与帕司烟肼单体MIC、MBC值无显著差异；动物实验表明该药应用安全。结论 帕司烟肼凝胶具有与帕司烟肼单体相同的抗结核菌药效，卡波姆基质不影响帕司烟肼的抗菌活性；以卡波姆为基质的帕司烟肼凝胶应用安全。     

性病后非病原体性症状的临床分析

目的了解性病后非病原体性症状的性质特点。方法采用临床会谈、心理咨询、SCL—90、SAS、SOS等相关量表调查测评。结果纳入分析病人134例，首诊均作实验室检查培养，病原体检出率为零，体格检查无器质性病变，符合非病原体性症状诊断。结论此类“症状”实质上是患者因患性病导致心理障碍面出现的综合症，而“症状”并无器质性及病原体因素，医务人员应更新观念，提高自身素质解除患者心理障碍。