An observational study of clinical recurrence in patients with interstitial lung disease related to the antisynthetase syndrome

Clinical Rheumatology - To describe the clinical characteristics and risk factors of clinical recurrence in interstitial lung disease related to antisynthetase syndrome (ARS-ILD). Patients...     

Facet joint plus interspinous process graft fusion to prevent postoperative late correction loss in thoracolumbar fractures with disc damage: finite element analysis and small clinical trials

Background

The biomechanical mechanism of spinal three-column after interspinous process fusion remains unclear. The goal of this study is to assess the biomechanics and clinical effects of facet joint plus interspinous process graft fusion on preventing postoperative correction loss in thoracolumbar fractures with disc damage treated with posterior approach.

Methods

By simulating internal fixation device removal postoperatively, two surgical finite element models of the L1–L2 segments for facet joint plus interspinous process fusion (treatment group model) and single-level facet joint fusion (control group model) were established and compression, flexion and extension were modeled on the basis of spinal three-column theory. The radiologic follow-up of a small prospective randomized controlled trial for the treatment group and control group was done to detect the clinical effects of these two surgical models.

Results

The disc compressive displacement and strain of the treatment group model were significantly reduced as compared to those of the control group model, the stress level on facet joint bone graft was also decreased. The posterior tension band of the treatment model was stronger and more stable than that of the control model. Accordingly, clinical trial results at postoperative late stage of the treatment group were significantly better than those of the control group, which had statistically significant difference (P < 0.05).

Interpretation

Facet joint plus interspinous process fusion is able to model the three-dimensional spinal stability more effectively than single-level facet joint fusion and is superior in bony fusion to prevent postoperative late correction loss in thoracolumbar fracture surgery.     

中西医结合治疗粉碎性Colles骨折

目的观察复位后中立位固定2周后改旋后位固定治疗粉碎性Colles骨折的临床疗效。方法100例粉碎性c011es骨折患者随机分为治疗组和对照组各50例,观察两组患者整复前后的腕部影像学指标（掌倾角、尺倾角、侧方和短缩移位）。结果两组患者治疗后,治疗组影像学指标改善较对照组明显,差异有统计学意义（P〈0．05）。结论早期中立位固定,2周后改为旋后位固定治疗粉碎性Colles骨折的疗效确切。     

钉螺细胞凋亡的诱导及检测研究

目的：研究多种物理或化学诱导条件下钉螺是否发生细胞凋亡现象以及比较两种检测方法。方法：细胞凋亡诱导剂或紫外线处理离体钉螺细胞以及40℃环境温度或灭螺药处理整体钉螺，用Annexin V-FITC试剂盒和DNA凝胶电泳法检测凋亡细胞，结果：钉螺在40℃环境中干放4h的诱导条件下，用Annexin V-FITC试剂盒检测出凋亡细胞；DNA凝胶电泳检测未成功，结论：首次诱导并观察到钉螺细胞凋亡现象。     

胸腰椎损伤影像学三维定位综合分类的建议

目的 :探讨胸腰椎损伤后影像学三维定位综合分类的方法。方法 :对 2 2 7例胸腰椎损伤患者的X线片、CT片于矢状面、额状面、水平面上对椎体、椎管、附件结构损伤状况进行测量研究 ,探讨不同骨折类型与损伤层面、椎管占位状态及三柱损伤的关系 ,并用一种简明的文字清楚表达其内涵。结果 :爆裂损伤以Ⅰ、Ⅱ、Ⅲ层面损伤 ,中央椎管占位 ,三柱均损伤为著 ;压缩骨折以Ⅰ、Ⅱ层面损伤 ,前柱损伤为著 ,无明显椎管占位 ;骨折脱位型以Ⅰ、Ⅱ、Ⅳ层面损伤最多 ,椎管占位指数最高。结论 :用简明文字表达胸腰椎损伤的三维定位综合分类方法可减少命名的复杂性和混乱性 ,为临床选择治疗方案提供可靠依据。     

结核分枝杆菌快速培养基临床应用研究

目的 评价分枝杆菌变色液体培养基快速检测痰标本结核分枝杆菌的可靠性.方法 将230例疑似肺结核患者痰标本接种于分枝杆菌变色液体培养基和酸性罗氏培养基培养,进行对照平行测定.结果 230例痰涂片标本检出阳性率为27.8%;变色液体培养基和酸性罗氏培养基培养阳性率分别为47.4%,47.0%,两种方法阳性率比较差异无统计学意义(P>0.05).结论 分枝杆菌变色液体培养基检测结核分枝杆菌具有特异、快速、可靠等优点,对结核病患者的早发现、早治疗具有积极意义.     

睡眠呼吸暂停综合征患者尿尿酸,心钠素,肾素—醛固酮的？…

目的 通过对睡眠呼吸暂停综合征（ＳＡＳ）患者的尿尿酸变化与夜间呼吸紊乱的关系及经鼻持续气道正压（ｎＣＰＡＰ）对其影响的研究,寻找一种监测ｎＣＰＡＰ长期疗效的简便有效的方法。同时研究ＳＡＳ患者的心钠素（ＡＮＰ）、肾素、醛固酮水平的变化及ｎＣＰＡＰ治疗与ＳＡＳ的夜间多尿的关系。方法 选取经多导睡眠图（ＰＳＧ）确诊为ＳＡＳ的患者２２例为试验组,１１名ＰＳＧ检查下沉者为姐,１３例重度ＳＡＳ患者接受ｎＣＰＡ     

日本血吸虫多价分子疫苗的保护性免疫力研究

本实验将从日本血吸虫成虫分离纯化出的２８ｋＤａ、３１／３２ｋＤａ及９７ｋＤａ蛋白混合组成多价分子疫苗免疫小鼠,再行攻击感染,观察其保护性免疫力。结果：疫苗加佐剂组成虫减虫率、肝组织减卵率及肠壁组织减卵率分别为５０．３％、７２．０％和８１．５％,均显著高于对照组（Ｐ＜０．０１）;实验组肝虫卵肉芽肿较对照组炎症反应程度轻,虫卵肉芽肿周长及面积均低于对照组（Ｐ＜０．０１）。结果提示,由日本血吸虫成虫２８ｋＤａ、３１／３２ｋＤａ及９７ｋＤａ蛋白联合组成的多价分子疫苗,具有较高的减虫率、减卵率及抑制虫卵肉芽肿形成的作用,有一定的应用前景     

佐剂对日本血吸虫31/32kDa蛋白保护性免疫力的影响

本实验用纯化的日本血吸虫３１／３２ｋ Ｄａ 蛋白（ Ｓｊ３１／３２ 蛋白）辅以ｐｏｌｙａｌｐｈａｏｌｅｆｉｎ（ Ｐ Ａ Ｏ）佐剂免疫小鼠,同时比较了 Ｓｊ３１／３２ 蛋白辅以福氏完全佐剂（ Ｆ Ｃ Ａ）对小鼠保护性免疫力的影响。 Ｓｊ３１／３２ 蛋白＋ Ｐ Ａ Ｏ 组小鼠减虫率为３７５５％ ,肝减卵率为６７０２％ ; Ｓｊ３１／３２ 蛋白组小鼠减虫率为２１１８％ , 肝减卵率为 ４９０３％ ; Ｓｊ３１／３２ 蛋白＋ Ｆ Ｃ Ａ 组小鼠减虫率为 ２４０２％ , 肝减卵率为５２２４％ 。结果提示, Ｓｊ３１／３２ 蛋白辅以 Ｐ Ａ Ｏ 佐剂的减虫作用优于 Ｓｊ３１／３２ 辅以 Ｆ Ｃ Ａ 的减虫作用（ Ｐ＜ ００５）,且明显强于单纯 Ｓｊ３１／３２ｋ Ｄａ 蛋白的保护性免疫作用（ Ｐ＜ ００１）。     

日本血吸虫成虫31/32kD蛋白对免疫小鼠TNF-α及sIL-2R影响的研究

为了研究Ｓｊ３１／３２所诱导的保护性免疫机制，探讨细胞因子ＴＮＦ－α及ＩＬ－２与Ｓｊ３１／３２保护力之间的关系，本实验用纯化的Ｓｊ３１／３２免疫小鼠，攻击感染后每隔２ｗｋ取外周血观察ＴＮＦ－α与ｓＩＬ－２Ｒ水平的动态变化。结果表明，Ｓｊ３１／３２可影响宿主ＴＮＦ－α与ＩＬ－２水平的表达。由此推测，Ｓｊ３１／３２的保护性免疫机制可能与宿主体内ＴＮＦ－α及ＩＬ－２水平的变化有关。