血塞通注射液对鼠肝CYP3A体外抑制作用研究

目的：研究血塞通注射液对大鼠肝微粒体CYP3A酶活性的体外抑制作用。方法：在大鼠肝微粒体体外孵育体系中加入CYP3A酶的底物睾酮和不同体积的血塞通注射液,用高效液相色谱法测定睾酮的羟化代谢产物6β-羟基睾酮的生成量反映CYP3A酶的活性。酮康唑为阳性对照药。结果：在大鼠肝微粒体体外孵育体系中,血塞通注射液对CYP3A酶的IC50和Ki值分别为血塞通注射液原液的0．87％和0．43％。结论：血塞通注射液在体外对大鼠肝微粒体CYP3A酶活性有抑制作用,符合混合型抑制模型。     

洲垸地区分疫源地类型优化血吸虫病防治对策的研究

本文报道在流行因素很复杂的洲垸地区，即江汉平原和洞庭湖区，选择8个村作为试区，通过查明血吸虫病流行特点，分疫源地类型优化防治对策，在提高人群和家畜化疗针对性的同时，因地制宜结合水利与农业工程改造疫源地环境。经连续多年观察，6个试区已达到控制流行的目标，2个试区居民感染率下降了63．78％以上。     

Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection

Concomitant infections of different species of parasites are common in the field. Infection with one parasite species likely triggers host responses that may influence the subsequent infection of another species and alter disease outcomes. So far, the majority of studies have focused on single species parasite infection, and the mechanisms of protection induced by the first parasite infection against the secondary infection remain poorly defined. In this study, we assess the impact of trematode Clonorchis sinensis infection on the course of another tissue nematode Trichinella spiralis challenge. We observed that mice with preexisting C. sinensis infection had lower worm burden of intestinal T. spiralis than those infected with T. spiralis alone; mice with preexisting C. sinensis also had severe enteric histopathological changes and higher counts of intestinal Paneth cells in responses to T. spiralis challenge. The mRNA levels of interleukin (IL)-4, IL-10, IL-13, and tumor necrosis factor (TNF)-α from the small intestine and spleen of the different groups were analyzed using quantitative real-time polymerase chain reaction. Compared with that in mice infected with T. spiralis alone, the mRNA expression of IL-13 was significantly increased in the small intestine tissues and IL-4, IL-13, and TNF-α were significantly increased in the spleen tissues in the dually infected mice. Our findings suggest that a “preexisting” trematode infection of C. sinensis is a factor which contributes to reducing the establishment of T. spiralis adult worms in the small intestine.     

空气喷磨机去除窝沟龋的扫描电镜观察

使用美国空气喷磨机（ＫＣＰ１０００），选用２７μｍ颗粒，８．２６８×１０＾５Ｐａ（１２０ｐｓｉ）气压，喷磨时间５～１０ｓ作为实验参数喷除新鲜离体人牙Ｈｅ面窝沟龋，扫描电镜观察显示，ＫＣＰ去龋干净，形成的釉质窝洞线角及洞缘圆钝，洞底粗糙不平，呈密集，不规则的凹凸状结构，实验结果提示ＫＣＰ能有效去除窝沟龋，产生的形态学改变特别适合应用复合树脂的粘接。