Developmental expression of sideroflexin family genes in Xenopus embryos

Emerging evidence indicates that mitochondrial carriers are not only crucial for metabolism, but also important for embryonic development. Sideroflexin is a novel family of mitochondrial tricarboxylate carrier proteins, of which the in vivo function is largely unknown. Here, we report on the expression patterns of five sideroflexin genes in Xenopus embryos. Whole‐mount in situ hybridization analysis reveals that while sideroflexin2 is expressed in the pancreas, sideroflexin1 and 3 display a complex expression in the central nervous system, somites, pronephros, liver, and pancreas. In contrast, only a weak expression of sideroflexin4 and 5 was detected in embryonic brain. Taken together, the five sideroflexin genes show both overlapping and nonoverlapping expression during Xenopus embryogenesis. As the primary structures of the five sideroflexin proteins are also quite similar, their functional redundancy should be taken into consideration for gene targeting studies. Developmental Dynamics 239:2742–2747, 2010. © 2010 Wiley‐Liss, Inc.     

The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection

Due to its delayed fluorescence of a lanthanide chelate, high accuracy and low background the broad linear range, long fluorescent life-time and large Stoke's shift of europium chelates, the time-resolved fluorescence has been developed for higher sensitive immunoassay. In this article, a simple, sensitive and specific method-time-resolved fluoroimmunoassay (TRFIA) was adopted for immunoassay of clonorchiasis, and recombinant glutathione transferases 2 of Clonorchis sinensis (rCsGST2) was used as a diagnostic antigen. To evaluate this novel assay for clinical applications, 409 serum samples were investigated. The diagnostic accuracy of the antigen was evaluated by receiver-operating characteristic (ROC) analysis. The area under the ROC curve (AUC) was 0.965, 95 % confidence interval (CI, 0.946, 0.985). To eliminate the random influence of ambient temperature, test parameters, photometric instruments and so on, the cut-off value was expressed as ratios between the fluorescence of sample and that of a well-defined negative control serum, and the deduced cut-off value was 9.3605. At the optimum cut-off criteria, the technique has a sensitivity of 95.80 %, specificity of 93.60 %. And the cross reactivity revealed that its cross reactivity with Schistosoma japonicum, round worm, hook worm, whip worm, and Toxoplasma gondii was 9.3, 8.3, 7.6, 9.8, and 5.0 %, respectively. Kappa score of agreement between TRFIA and microscopic examination of stools was 0.892, P?<?0.05. These combined results showed that our method is feasible and could be used for the clinical determination of clonorchiasis.     

后侧入路治疗肱骨中远段骨折合并桡神经损伤的临床分析

目的探讨后侧入路治疗肱骨中远段骨折合并桡神经损伤的临床效果,以期提高临床治疗水平。方法 2009-01—2013-01选取70例肱骨中远段骨折合并桡神经损伤患者为研究对象,随机分成2组,对照组予以前外侧入路,观察组予以后侧入路,比较2组在手术时间、失血量、桡神经损伤率和临床效果方面的差异。结果从总疗效情况看,对照组无效率20%,总有效率80%;观察组无效率5.71%,总有效率94.29%,2组比较差异有统计学意义(P<0.05);从神经恢复上看,对照组轻度损伤20例(57.15%),中度损伤9例(25.71%),重度损伤6例(17.14%)。观察组轻度损伤28例(80%),中度损伤4例(11.43%),重度损伤3例(5.71%),2组比较差异有统计学意义(P<0.05);2组失血量、生活质量评分、骨折延迟愈合情况、桡神经医源性损伤情况、手术时间比较差异有统计学意义(P<0.05)。结论后侧入路治疗肱骨中远段骨折合并桡神经损伤,视野清晰,桡神经解剖暴露满意,对桡神经移位能减少损伤,后侧固定符合张力带原则,有利于肱骨骨折的固定。     

经皮穿刺内固定治疗上颈椎骨折与不稳

目的:探讨经皮穿刺内固定治疗上颈椎骨折与不稳的方法与效果。方法:对40例20～45岁正常人C1、C2行CT扫描,测量C1、C2侧块的标准角、安全角、椎动脉内壁至标准角距离、齿状突基底冠状径、矢状径、齿状突长度、枢椎总高度及齿状突轴心线与C3椎体前缘夹角等相关数据。采用自行设计的器械行经皮穿刺内固定治疗上颈椎骨折与不稳47例。其中齿状突骨折21例,Jefferson骨折7例,C1前弓骨折4例,寰枢椎脱位及本脱位15例,分析其治疗结果。结果:C1、C2侧块在正位投照上的标准角右侧为(24.0±3.7)°,左侧为(23.8±1.8)°。安全角右侧为15.2°～30.3°,左侧为14.8°～32.1°。椎动脉内壁至寰椎侧块上下缘中点连线的距离右侧为(5.6±2.2)mm,左侧为(5.8±1.9)mm。侧位投照像上,标准角为(24.1±18)°,安全角为12.6°～26.8°。齿状突基底冠状径为(8.8±1.2)mm,矢状径为(10.9±1.0)mm,齿状突长度为(14.2±1.2)mm,枢椎总高度为(38.2±1.8)mm,齿状突轴心线与C3椎体前缘夹角为(23.1±1.4)°。全组病例平均随访18个月,未发现脱钉、弯钉及断钉。齿状突螺钉固定17例,除2例齿状突骨折未愈合外。余均获骨性愈合。C1、C2侧块螺钉固定治疗者中26例颈椎旋转活动比正常减少40°,伸屈正常。4例颈部强直感,未恢复原有工作。结论:经皮穿刺颈     

高原寒区战时环境猪肢体枪弹伤简化初期外科处理原则

目的研究高原寒区战时环境下枪弹伤后伤道初期外科处理原则。方法 108头小型猪随机分为高原战时致伤传统清创延期缝合固定组（GCT）、高原战时致伤一期清创固定缝合组（GYQ）、高原战时致伤简单清创引流延期缝合外固定架固定组（GJD）3组,分别于每组伤后6、12、24、36、48、72 h进行相应的初期外科处理。结果 （1）高原战时环境伤道组织肉眼可辨的炎性分界时间为伤后12~24 h;（2）伤道组织在伤后24 h明显肿胀,36~48 h肿胀达高峰,清创后7 d组织肿胀显著消退;（3）伤道感染最早发生在伤后24 h,伤后12~24 h清创细菌清除率最高;（4）GYQ组各时相点初期外科处理的伤道局部总感染率都很高,GCT组和GCT组36 h以后各时相点处理的局部总感染率均很高,GJD组在伤后的6~24 h初期外科处理的未见感染发生,36 h以后各时相点的局部总感染率与GCT组相似;（5）伤道闭合后28 d解剖动物发现,局部感染动物大多有全身脓毒血症,在肺、肝、脑、肾等处均有广泛的散在的脓肿形成,早期死亡动物在肺、肝、肾等脏器也可见广泛的出血点。GCT组和GYQ组早期死亡率较高,GJD组在任一时相点均未见死亡动物。结论高原战时环境肢体枪弹伤不行一期缝合、不做骨折一期内固定,伤后早期不宜扩大清创范围,伤后6~24 h实施简单初期外科处理是可行的,伤口延期缝合较佳时机在清创后7 d,应尽早应用外固定架进行骨折有效固定。     

补肾固本方对大鼠骨质疏松模型中PI3K/AKT/mTOR信号通路表达的调控研究

目的观察补肾固本方对去卵巢骨质疏松(osteoporosis,OP)大鼠骨组织中PI3K/AKT/mTOR信号通路的影响,探讨补肾固本方干预OP的作用机制。方法将100只SD大鼠随机分为5组:正常组(C组)、模型组(M组)、补肾固本方组(B组)、补肾固本方+PI3k受体特异性阻断剂LY294002组(B+L组)、PI3k受体特异性阻断剂LY294002组(L组),除正常组外,其余4组建立去卵巢大鼠OP模型。药物连续干预12周后,采用ELISA方法检测血清中E2、BGP、ALP水平,实时荧光定量聚合酶链式反应(Real-time PCR)检测各组股骨组织中LC3、Beclin1、caspase-9 mRNA的表达情况,蛋白质印迹法(Western blot)检测各组股骨组织中PI3K、AKT、mTOR蛋白的表达。结果补肾固本方可显著增高OP大鼠血清E2水平,降低BGP、ALP水平,显著上调LC3、Beclin1表达水平,降低caspase-9、PI3K、p-AKT、mTOR的表达;而上述变化能够被PI3k受体特异性阻断剂LY294002所阻断,且其差异具有统计学意义(P0.05)。结论补肾固本方减少去卵巢后大鼠OP的发生,其机制可能与抑制PI3K/AKT/mTOR信号通路及其下游基因蛋白表达有关。     

股骨头部分置换术精准微创治疗中老年ARCO Ⅲ期股骨头缺血坏死的有限元分析

目的通过有限元分析,初步评估股骨头部分置换术后股骨与内植物模型的应力分布和位移。 方法选择一名成年健康志愿者,获取股骨全长CT扫描数据,利用Mimics 20.0软件、Geomagic软件及UG NX 12.0软件建立股骨头缺血坏死模型,骨隧道切除股骨头坏死区域后装配设计开发的股骨头假体,模拟单腿站立环境进行有限元仿真模拟,获取股骨与内植物模型的应力分布和位移数据。 结果股骨应力分布主要集中在股骨颈下方和股骨干皮质骨两侧,最大应力为48.25 Mpa,最大位移为10.98 mm。内植物模型的应力主要分布在金属内植物主体结构下方,最大应力为147.2 Mpa,最大位移为9.58 mm。 结论股骨头部分置换后,假体头与股骨头曲率一致,应力传导模式与正常侧髋关节一致,但在假体头与茎部连接处发生应力集中,应重点考虑选择更大强度的材料。     

牵张性脊髓损伤脊髓SCEP监护作用的实验研究

目的：探讨脊髓皮层诱发电位对牵张性脊髓损伤的监护作用。方法：选用４０只健康大白兔，随机分成对照组（Ａ组）、体感皮层诱发电位（ＳＣＥＰ）波幅下降３０％组（Ｂ组）、ＳＣＥＰ波幅下降５０％５ｍｉｎ组（Ｃ组）和１０ｍｉｎ组（Ｄ组）。通过对脊髓ＳＣＥＰ监测、动物脊髓功能评定、组织形态学观察以及脊髓微血管铸型扫描电镜观察来研究牵张性脊髓损伤。结果：随着牵开负荷增大和作用时间的延长，脊髓微血管发生充盈缺损、痉挛直至破裂出血；Ｃ组及Ｄ组脊髓功能下降，与Ａ组相比有显著性差异。结论：ＳＣＥＰ波幅变化能较客观地反映脊髓功能状况，波幅下降５０％持续１０ｍｉｎ脊髓将出现不可逆损害