中国国家出生队列研究调查对象的基线特征分析

目的 探讨生命早期环境因素、遗传因素和遗传与环境交互作用对子代近期和远期健康的影响以及系统评价和比较辅助生殖受孕和自然受孕人群的妊娠结局和子代健康相关结局。方法 中国国家出生队列（CNBC）研究是一项覆盖辅助生殖受孕家庭和自然受孕家庭的多中心前瞻性出生队列研究。2016年,CNBC项目陆续在我国12个省（自治区、直辖市）的24所医院启动,以家庭为单位纳入研究对象,并在辅助生殖治疗前、胚胎移植、孕早期、孕中期、孕晚期及分娩时以及出生后第42天、6个月、12个月、36个月多个时点采集数据信息和生物样本。结果 截至2020年6月,CNBC共纳入27 044个辅助生殖受孕家庭,29 589个自然受孕家庭,CNBC的研究人群中绝大部分为城市居民。在辅助生殖受孕家庭中,男女双方分别有65.5%和63.7%为大学及以上文化程度,年龄为（33.83±5.52）和（32.38±4.67）岁;女方83.2%为初产妇,吸烟率为0.8%,饮酒率为2.1%。在自然受孕家庭中,男女双方分别有81.5%和86.5%为大学及以上文化程度,年龄为（32.06±5.09）和（30.40±4.27）岁,女方67.2%为初产妇,吸烟率为0.1%,饮酒率为2.2%。不同地区的辅助生殖受孕家庭和自然受孕家庭的基线特征均有差异。结论 CNBC的建立将为研究生命早期遗传、环境因素、遗传-环境交互作用以及辅助生殖技术治疗相关因素对出生后子代健康的影响提供了重要资源。     

The establishment of the Korean medical device safety information monitoring center: Reviewing ten years of experience

Medical devices may revolutionize healthcare delivery but can lead to serious adverse events for treated patients and users. While reporting of adverse events related to medical devices is an essential starting point for post-market surveillance, underreporting of medical device adverse events is a global problem. Korea introduced a voluntary medical device adverse event reporting system in 2010, called the Medical Device Safety Information Monitoring Center, which has led to an increase in adverse event reports. For 10 years, the Medical Device Safety Information Monitoring Center has analyzed medical device adverse events systematically and has provided active feedback to the manufacturers and education on safe use. Recently, the Medical Device Safety Information Monitoring Center contributed to harmonization of international medical device vigilance through the sharing of adverse events. This experience of Korea might contribute to improvements in medical device vigilance, which is a critical prerequisite for improving medical device policies and regulations.     

Two immunogenic recombinant protein vaccine candidates showed disparate protective efficacy against Zika virus infection in rhesus macaques

Zika virus (ZIKV) infection has caused major public health problems recently. To develop subunit vaccines for ZIKV, we have previously constructed recombinant ZIKV envelope protein domain III (EDIII), and the entire ectodomain (E80, which comprises EDI, EDII and EDIII), as vaccine candidates and showed both of them being immunogenic and protective in murine models. In this follow-up study, we compared these vaccine candidates in non-human primates. Both of them elicited neutralizing antibody responses, but only E80 immunization inhibited ZIKV infection in both peripheral blood and monkey tissues, whereas EDIII increased blood ZIKV RNA through possibly antibody-dependent enhancement. Further investigations revealed that the virion-binding antibody response in E80 immunized monkeys persisted longer and stronger than in EDIII immunized monkeys. These results demonstrate that E80 is superior to EDIII as a vaccine candidate, and that the magnitude, quality and durability of virion-binding neutralizing antibodies are correlates of protection.     

The prevalence of stroke and related risk factors among residents aged ≥ 40 years in Chongqing,Southwest China

Journal of Public Health - China bears the largest global stroke burden, yet little is known about its rates in Chongqing, southwest China. We aimed to investigate the prevalence and related risk...     

"健康中国"战略下临床医学专业本科生职业医学教育的实践与探索

临床医学专业学生是职业病的诊断、治疗、康复和预防的未来主干力量,因而在"健康中国"战略下采用多元化教学方式培养学生解决问题的能力,提高临床医学生学习职业医学的积极性,推进职业医学专业人才队伍建设,对维护全体劳动者身体健康、促进经济社会协同发展具有重要意义。     

分散固相萃取-气相色谱串联质谱法测定菊花茶中的56种农药残留

目的 建立菊花茶中56种农药残留的分散固相萃取（QuEChERS） - 气相色谱串联三重四级杆质谱（GC - MS/MS）测定方法。方法 样品用乙腈提取,PSA+C18+GCB混合型分散固相萃取剂净化,DB - 5MS色谱柱分离,多反应监测模式（MRM）检测,外标法定量。结果 在0.005～500 mg/L范围内,56种农药的线性相关系数均≥0.995,方法定量限为0.002～0.057 mg/kg,平均回收率为60.0%～118.0%,相对标准偏差（RSD）为0.53%～20.09%。结论 该方法简单快速灵敏,可以实现菊花茶中农药残留的快速高通量检测。     

甘孜州12岁藏族儿童牙周健康现状及其相关因素分析

目的 了解四川省甘孜州地区12岁藏族儿童的牙周疾病情况及相关影响因素。方法 采用多阶段分层随机抽样的方法,在甘孜州地区选取12岁藏族儿童共489名,对其牙周情况进行检查,并进行问卷调查,采用Mann-Whitney U检验和卡方检验比较数据差异,对牙周疾病危险因素采用多因素logistic回归分析。结果 甘孜州藏族儿童的牙石平均检出率和平均检出牙数为82.8%、6.06;平均牙龈出血率和平均检出牙数为75.5%、6.25。男生牙石和牙龈出血情况均比女生严重(P<0.05)。城市儿童牙龈出血率比农村高(P<0.05)。多因素logistic回归结果提示,拥有兄弟姐妹是牙龈出血的保护因素(一个: OR=0.230, 95%CI: 0.100~0.550 二个: OR=0.310, 95%CI: 0.110~0.870 三个及更多: OR=0.150, 95%CI: 0.050~0.480),更高的父亲学历是牙龈出血的保护因素(OR=0.290, 95%CI:0.100~0.820)。每周饮用甜饮料是牙石的危险因素(OR=3.870, 95%CI:1.540~9.750)。 结论 甘孜州12岁藏族儿童牙周患病情况不容乐观,需加强针对这一人群的口腔预防保健干预。     

骶管内蛛网膜囊肿的诊断及治疗体会

目的探讨骶管内蛛网膜囊肿的发病机理、临床特点及治疗方法.方法 9例患者经MRI检查确诊,全部采取手术摘除治疗.结果随访9例,时间8月～5年,平均2.8年,手术摘除治疗骶管内囊肿的疗效满意,随访中未发现囊肿复发和神经损伤再加重.结论骶管内囊肿是相对常见的疾病,好发于40岁以上人群,与退变性腰椎疾病的临床表现有很大的相似处,易误诊;本病最好的诊断方法是行腰骶椎MRI检查;治疗以手术切除囊肿壁为主,具有较好的疗效.     

一种快速简便的缺失突变方法

为了构建能表达稳定的、不易降解的ＴＭ－ＴＮＦ突变体（ＴＭ－ＴＮＦｍ）的基因，本文用改良的Ｓ－ＰＣＲ和ＯＥ－ＰＣＲ两种定位突变技术，成功地构建了缺失３６个碱基的ｐＢＳＫ－ＴＭ－ＴＮＦ突变重组体。与ＯＥ－ＰＣＲ技术（用两对引物进行两次ＰＣＲ反应）相比较，Ｓ－ＰＣＲ技术（用一对引物做一次ＰＣＲ反应）具有快速、经济、简便等优点，但其突变率较ＯＥ－ＰＣＲ低。