郁证与虚证的关系初探

历代对郁证的认识,详于实证,而略于虚证。王彦晖教授擅长中医肝胆病治疗,对虚证与郁证的关系有独到的理解,认为肝肾阴虚是郁证的基础病因。治疗上,根据伤阴的层面不同,分为阴液不足,阴血亏虚证与阴精匮乏3类,分别采用凉润养阴、温养阴血和填补肾精之法,在临床取得了良好效果。     

需长期应用糖皮质激素患者出院健康教育需求调查

目的了解需长期应用糖皮质激素的患者出院时对健康教育知识的需求和出院后健康教育方式的选择。方法自制问卷，采用访谈式调查法对90例患者于出院前1～2d进行调查。结果需长期应用糖皮质激素的患者比较注重药物有关信息，但忽视自我护理方面的相关知识，护士应予正确引导；对出院后健康教育方式的选择中电话咨询受到青睐。结论护士要根据患者对健康教育需求的特点有针对性地进行健康教育，对其忽视的方面给予正确引导。     

网络环境下的医院药学信息服务工作实践

目的:利用网络信息优势,促进医院药学信息服务工作发展。方法:利用医院HIS系统建立局域网药学信息服务平台及建立药学网站开展药学信息服务工作。结果:形成网络环境下的医院药学信息服务新模式。结论:网络环境下的医院药学信息服务工作更加科学与扎实,在医院药学的发展中将有更重要的地位。     

Sympathetic nervous system, genes and human essential hypertension

The sympathetic nervous system (SNS) is the first line of defense in the response to environmental stress through its regulation of second-to-second changes in blood pressure (BP). Both the activity of the SNS and the therapeutic responses to SNS agonists and antagonists are known to be highly variable in the population. "Small" changes caused by single nucleotide polymorphisms (SNPs) of SNS genes may have considerable impact on SNS function and individualized hypertension treatment. In this review, we first describe the physiology of the SNS and its influence on cardiovascular and renal mechanisms of BP regulation. A thorough review of the role of genetic variability of various SNS genes in relation to the development of BP and essential hypertension (EH) follows. Given the vast number of SNS components, evaluations of multiple SNPs from multiple SNS genes are necessary for future association studies of BP and EH. One way to surpass the limitations and inconsistencies of previous association studies is to use a gene-based approach also referred to as indirect association, which takes all common variation within a candidate gene into account. In order to determine how SNS genes are differentially expressed or silenced, activated or inactivated against various environmental backgrounds, it is important to assess not only environmental and lifestyle risk factors such as diet, climate, chronic stress, but also personality characteristics such as hostility and coping styles. Uncovering relevant gene-gene and gene-environment interactions within the SNS cascade will not only enable early detection of EH risk but will also aid in the treatment of hypertensives through both non-pharmacological and pharmacological means.     

Combined optimal-pathlengths method for near-infrared spectroscopy analysis

Near-infrared (NIR) spectroscopy is a rapid, reagent-less and nondestructive analytical technique, which is being increasingly employed for quantitative application in chemistry, pharmaceutics and food industry, and for the optical analysis of biological tissue. The performance of NIR technology greatly depends on the abilities to control and acquire data from the instrument and to calibrate and analyse data. Optical pathlength is a key parameter of the NIR instrument, which has been thoroughly discussed in univariate quantitative analysis in the presence of photometric errors. Although multiple wavelengths can provide more chemical information, it is difficult to determine a single pathlength that is suitable for each wavelength region. A theoretical investigation of a selection procedure for multiple pathlengths, called the combined optimal-pathlengths (COP) method, is identified in this paper and an extensive comparison with the single pathlength method is also performed on simulated and experimental NIR spectral data sets. The results obtained show that the COP method can greatly improve the prediction accuracy in NIR spectroscopy quantitative analysis.     

黄芩素能下调诱捕受体3表达抑制肝癌干细胞的生物学行为

文题释义： 诱捕受体3：1998年Pitti在搜索表达序列标签数据库时发现一组与肿瘤坏死因子受体超家族成员同源的表达序列标签,诱捕受体3基因位于人染色体20q 13.3,已知此区域在人类肿瘤发生过程中与基因的扩增和重组有关。 肿瘤干细胞：与正常组织一样,肿瘤组织由处于各种不同分化程度的细胞组成,其中存在一部分独特的具有自我更新和分化功能的干细胞样亚群并将之称为肿瘤干细胞,又称肿瘤起始细胞或成瘤性癌细胞。 背景：目前尚未见有关以肝癌干细胞为靶向的中草药抗肝癌机制的报道,因此有必要展开相关研究。 目的：探讨黄芩素对肝癌干细胞诱捕受体3表达的影响,以及下调诱捕受体3表达对肝癌干细胞生物学行为的影响。 方法：按照课题组之前建立的技术方法,从肝癌细胞株(购自中国科学院上海细胞库)中获得肝癌干细胞。将肝癌干细胞分为黄芩素高、中、低剂量组以及对照组,黄芩素高、中、低剂量组分别用含200,100,50 μmol/L黄芩素的L-DMEM培养基培养,对照组仅用L-DMEM培养基培养。采用RT-PCR、Western blot检测黄芩素处理后诱捕受体3 mRNA和蛋白表达变化,采用CCK8法、流式细胞术、Transwell法检测肝癌干细胞增殖、细胞周期分布、凋亡和迁移情况。 结果与结论：①与对照组相比,高剂量黄芩素能显著下调肝癌干细胞诱捕受体3的mRNA和蛋白表达,因此确认高剂量黄芩素为最佳作用浓度;②与对照组相比,高剂量黄芩素组肝癌干细胞的增殖能力明显下降,S期和G₂期细胞比例增加,而G₁期的细胞比例则减少(P < 0.05);③与对照组相比,高剂量黄芩素组肝癌干细胞的凋亡率明显增加,迁移能力明显下降;④结果表明,高剂量黄芩素能通过下调肝癌干细胞的诱捕受体3表达而抑制细胞的一系列生物学行为,为临床上以诱捕受体3为靶点,以肝癌干细胞为对象进行肝癌治疗提供了实验依据。 ORCID: 0000-0002-6020-0616(岑妍慧) 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

结构性授权理论及其对护士工作满意度影响的研究进展

介绍了结构性授权理论,该理论包括机会权力、信息权力、支持权力、资源权力4个基本要素,结构性授权可以通过两种不同的体系来获得,即正式授权和非正式授权。综述了国内外结构性授权对护士工作满意度的影响,以期通过对研究现状进行评析的基础上,结合我国卫生保健系统的实际情况进行护理管理结构性授权的研究。     

爱尔卡因表麻下白内障超声乳化吸出术临床观察

目的：探讨表麻下行白内障超声乳化吸出及人工晶体植入术的可行性并对其效果进行评估。方法：随机选择手术病例共１１５例１１７只眼进行表麻下白内障超声乳化吸出及人工晶体植入术,但除外儿童、复杂白内障及不合作者,观察麻醉效果、术中术后并发症、术前术后视力对照等。结果：１１７只眼表麻成功率９７．４％,术中术后并发症、术后视力、Ｐｈａｃｏ参数等均与常规麻醉无大差异。结论：表麻是白内障手术一大进步,其优点是安全性     

南珠水解液对H2O2诱导的人晶状体上皮细胞氧化损伤的保护作用

目的探讨南珠水解液对过氧化氢(H₂O₂)诱导的人晶状体上皮细胞(HLEC)氧化应激损伤的保护作用。方法培养HLEC细胞株HLEB3,在培养液中加入不同浓度南珠水解液,培养24 h和48 h分别检测细胞增殖水平,并据此选择后续实验中南珠水解液浓度。培养HLE-B3细胞,分成3组:正常对照组;氧化损伤组,加入H₂O₂至终浓度为300μmol·L^-1;南珠水解液处理组,加入H₂O₂至终浓度为300μmol·L^-1,加入南珠水解液至终浓度为200 mg·L^-1。3组细胞使用流式细胞术检测细胞凋亡率,利用电镜观察细胞形态差异;利用生化检测试剂盒检测3组细胞中谷胱甘肽过氧化物酶(GSH-Px)和超氧化物岐化酶(SOD)活性以及丙二醛(MDA)含量。结果 3组细胞增殖法检测结果显示,南珠水解液处理HLE-B3细胞24 h后,对其活性无抑制作用;50 mg·L^-1、100 mg·L     

A novel standardized test system to evaluate dynamic visual acuity post trifocal or monofocal intraocular lens implantation: a multicenter study

ObjectivesTo compare the dynamic visual acuity (DVA) following implantation of trifocal with monofocal intraocular lenses (IOL) and using a novel test system.MethodsThe present research was a retrospective, multicenter clinical study. Two hundred and ten eyes of 149 patients that underwent cataract phacoemulsification and IOL implantation were enrolled. One hundred and ten eyes of patients received trifocal (AT LISA tri839MP, Carl Zeiss Meditec, Germany) and 100 eyes received monofocal (Tecnis ZCB00, Abbott, United States) lenses and were evaluated 3 months after implantation. Outcome measures included monocular uncorrected distance (UDVA), intermediate (UIVA) and near (UNVA) visual acuity and best corrected distance visual acuity (BCDVA; logMAR units); contrast sensitivity under photopic, mesopic, with glare conditions; and dynamic visual acuity using a self-developed system.ResultsThere was no statistically significant difference in baseline characteristics between groups. Monocular UDVA, UIVA, and UNVA were significantly better (all p < 0.001) in the trifocal IOL group, but not BCDVA. The trifocal IOL group showed better contrast sensitivity under photopic, mesopic, with or without glare conditions (p < 0.05) and statistically significantly better dynamic vision at 15, 30, 60, and 90 degrees per second (dps) compared with the monofocal group (all p < 0.001). The strength of positive association only between UIVA and dynamic visual acuity increased as the velocity increased, but not UDVA or UNVA.ConclusionsDiffractive trifocal IOL provides better postoperative full range vision acuity, contrast sensitivity, and dynamic vision compared with monofocal IOLs. And intermediate visual acuity is increasingly associated with DVA as the velocity increasing.Subject terms: Quality of life, Outcomes research