慢性间歇低氧/复氧对大鼠骨代谢的影响

目的建立间歇低氧/复氧模型,观察间歇低氧/复氧暴露对SD大鼠骨代谢的影响并探讨其可能的发病机制。方法将20只8周龄SD雄性大鼠随机分为间歇低氧(IH)组和常氧对照(NC)组,每组10只放入间歇低氧模拟舱进行间歇低氧/复氧造模,8周后处死,测定骨代谢指标骨矿盐含量、血清B-ALP、各部位骨密度(BMD),氧化应激指标超氧化物歧化酶(SOD)和丙二醛(MDA)水平,炎症反应指标IL-6和TNF-α表达水平。结果与NC组相比,IH组B-ALP含量升高,全身BMD、股骨BMD和骨矿盐含量降低,差异均具有统计学意义(P0.05);SOD水平降低,MDA水平升高,差异均具有统计学意义(P0.05);IL-6和TNF-α表达水平升高,差异均具有统计学意义(P0.05)。结论慢性间歇低氧/复氧条件下可能诱导SD大鼠体内发生氧化应激状态和炎症反应,引起大鼠骨质和相关代谢指标的变化。     

除湿通痹方对大鼠急性痛风性关节炎抗炎作用的实验研究

目的研究除湿通痹方对尿酸钠（MSU）诱导的急性痛风性关节炎模型大鼠足关节肿胀率及足关节滑膜细胞因子表达的影响,探讨该方治疗急性痛风性关节炎的部分作用机制。方法选用Wistar雄性大鼠40只,随机分成生理盐水组、阳性对照组、除湿通痹方I组、Ⅱ组。各组给予相应药物灌胃,2次/d,第5天灌胃1.5h后予尿酸钠晶体注射局部关节腔形成大鼠急性痛风性关节炎模型,并于不同时间测量大鼠足关节肿胀度,观察该方对大鼠足关节肿胀率的影响;注射尿酸钠24h后,处死大鼠并应用RT-PCR,检测对关节滑膜细胞因子TNF-α mRNA、IL-1βmRNA表达的影响。结果除湿通痹方可有效抑制大鼠踝关节肿胀程度;与生理盐水组比较,TNF-α mRNA 和 IL-1β mRNA的表达明显减低。结论除湿通痹方可抑制痛风性关节炎TNF-α和IL-1β的表达而发挥抗炎作用,明显减轻关节的病理性损伤,有效抑制关节炎的临床进程。     

Chronic morphine exposure alters the dendritic morphology of pyramidal neurons in visual cortex of rats

Repeated treatment of psychotropic drugs produces changes in brain and behavior that far outlast their initial neuropharmacological effects. The nature of persistent drug-induced neural plasticity is of interest because it is thought to contribute to the development of drug dependency and addiction. To determine if chronic morphine treatment alters the morphology of visual cortical neurons, we statistically examined the dendrites of layer III pyramidal neurons in the primary visual cortex of both morphine-treated and saline-control rats. Compared with control rats, the pyramidal cells of morphine-treated animals showed a significant decrease in the total dendritic length (24%) and a significant reduction (27%) in the dendritic spine density of dendritic arborization at the level of the second branch order. Our results suggest that some of the persistent neurobehavioral consequences and cognitive impairment resulting from repeated exposure to morphine may involve a reorganization of synaptic connectivity in visual cortical neurons.     

Development of new enzyme-linked immunosorbent assay for oxidized lipoprotein(a) by using purified human oxidized lipoprotein(a) autoantibodies as capture antibody

BACKGROUND: Oxidized Lp(a) [ox-Lp(a)] has been reported to play more potent role than native Lp(a) in atherosclerosis. Ox-Lp(a), autoantibodies, and Lp(a) immune complexes have all been detected in vivo. Thus, the isolation of its autoantibodies and the investigation of ox-Lp(a) may provide a new means to explore the exact pathogenic role of ox-Lp(a). We isolated and identified human autoantibodies against ox-Lp(a) and developed a new ELISA for ox-Lp(a) by using autoantibodies as capture antibody. METHODS: Ox-Lp(a) autoantibodies were isolated and identified from healthy subjects by affinity chromatography. 2 "sandwich" ELISAs were developed for measuring ox-Lp(a) level, using autoantibodies against ox-Lp(a) or rabbit antiserum against ox-LDL as the capture antibody and quantitating with monoclonal anti-apo(a) enzyme conjugate, respectively. Ox-Lp(a) levels were studied by both the ELISAs in 100 patients with coronary heart disease (CHD) and 100 control subjects. RESULTS: The isolated ox-Lp(a) autoantibodies reacted with both apo(a) and apoB epitopes of Ox-Lp(a). Compared to control, plasma ox-Lp(a) levels in patients with CHD were significantly increased (ELISA using human autoantibodies: 24.3+/-33.4 vs. 8.4+/-9.3 microg/ml, P<0.0001; ELISA using antibodies against ox-LDL: 13.0+/-13.8 vs. 7.3+/-9.7 microg/ml, P<0.0001, respectively). Furthermore, a significantly positive relationship between ox-Lp(a) levels detected by 2 ELISAs was also found (R=0.78, P<0.0001). CONCLUSION: We isolated human autoantibodies against ox-Lp(a), which can recognize both apo(a) and apoB epitopes of ox-Lp(a). The developed ELISA for ox-Lp(a) by using human auoantibodies may more accurately reflect the state of Lp(a) oxidation in vivo. Ox-Lp(a) levels increase in patients with CHD.     

60Co-γ辐射对元胡品质及根际菌群的作用

[目的] 研究60Co-γ辐射对元胡品质的直接影响及对根际微生态的间接影响。 [方法] 利用高效液相色谱(high performance liquid chromatography,HPLC)法和高通量测序检测不同剂量射线辐射对元胡品质的影响和辐射后根际细菌群落结构和多样性的变化。[结果] 随着60Co-γ辐射剂量的增加,元胡的鲜重、干重、粒径及膨大率均明显增加,但其主要药效成分的含量并未显著提升。研究元胡根际土壤的菌群发现,与无辐射组比较,辐射组的生物多样性指数更高,表明辐射会改变元胡根际菌群多样性,其中80 Gy辐射组的细菌多样性最高。从菌群组成来看,各组在细菌的门以及优势属的分类水平上差异无统计学意义(P＞0.05),而一些含量低于4%的非优势菌变化明显,其数量随辐射剂量增加而增加,表明元胡经过射线辐射后,根部菌群可能会发生变化,在根部富集一些新的细菌,从而提高细菌多样性。 [结论] 60Co-γ照射会显著影响元胡的产量和大小,但对药效成分的改变不显著。辐射可能会通过提高根际土壤的非优势菌水平,甚至富集一些新的细菌来提高根际土壤细菌多样性。综合来看,60～80 Gy是元胡的最佳诱变剂量。     

血清CA125、miRNA-139-5p、miRNA-15a水平检测在卵巢癌患者中的临床意义

目的:探讨血清糖类抗原125(CA125)、微小RNA-139-5p(miRNA-139-5p)、微小RNA-15a(miRNA-15a)水平检测在卵巢癌患者中的临床意义。方法:选取74例卵巢癌患者作为卵巢癌组,选取同期74名该院健康体检者作为对照组,比较卵巢癌组与对照组、不同分化程度卵巢癌患者、不同病理分期卵巢癌患者血清CA125、miRNA-139-5p、miRNA-15a水平,分析血清CA125、miRNA-139-5p、miRNA-15a水平与卵巢癌分化程度和病理分期的相关性。结果:卵巢癌组CA125、miRNA-139-5p水平均高于对照组,miRNA-15a水平低于对照组,差异有统计学意义(P<0.05);卵巢癌低分化患者血清CA125、miRNA-139-5p水平均高于中分化和高分化患者,且中分化患者高于高分化患者;卵巢癌低分化患者miRNA-15a水平低于中分化和高分化患者,且中分化患者低于高分化患者,差异有统计学意义(P<0.05);卵巢癌患者血清CA125、miRNA-139-5p水平随着病理分期升高而升高,即Ⅰ期<Ⅱ期<Ⅲ期<Ⅳ期,卵巢癌患者miRNA-15a水平随着病理分期升高而降低,即Ⅰ期>Ⅱ期>Ⅲ期>Ⅳ期,差异有统计学意义(P<0.05);Pearson相关性分析结果显示,CA125、miRNA-139-5p水平与卵巢癌分化程度呈负相关,与病理分期呈正相关,miRNA-15a水平与卵巢癌分化程度呈正相关,与病理分期呈负相关(P<0.05)。结论:卵巢癌患者血清CA125、miRNA-139-5p水平升高,miRNA-15a水平降低,且与卵巢癌分化程度、病理分期存在相关性,血清CA125、miRNA-139-5p、miRNA-15a水平可为临床卵巢癌患者诊治提供参考依据。     

弥漫性轴索损伤BDNF及其Val66Met基因多态性与认知功能的相关性研究

目的探讨弥漫性轴索损伤（DAI）（Ⅱ型）患者伤后1个月血清脑源性神经营养因子（BDNF）水平及其Val66Met基因多态性与认知功能的关系。 方法选取晋江市医院神经外科自2015年8月至2020年8月收治的106例DAI（Ⅱ型）患者为病例组,选择同期来本院体检的105名健康体检者为对照组,采用第二版洛文斯顿作业疗法认知量表（LOTCA）、蒙特利尔评估量表中文版（MoCA）分别评估对照组和病例组伤后1个月时的认知功能;采用酶联免疫吸附试验测定2组研究对象的血清BDNF水平;聚合酶链反应-限制性片段长度多态性分析BDNF Val66Met基因多态性;多元逐步回归法分析病例组整体认知功能与BDNF及BDNF Val66Met基因多态性的相关性。 结果病例组伤后1个月相同基因亚型血清BDNF浓度均低于对照组,差异有统计学意义（P<0.05）;病例组Val/Val亚型血清BDNF浓度高于Val/Met、Met/Met亚型,差异有统计学意义（P<0.05）,而Val/Met和Met/Met亚型血清BDNF浓度比较差异无统计学意义（P>0.05）。病例组患者3种基因亚型伤后1个月的LOTCA和MoCA评分均低于对照组,差异有统计学意义（P<0.05）;病例组Val/Val亚型评分高于Val/Met、Met/Met评分,差异有统计学意义（P<0.05）,而Val/Met和Met/Met亚型评分比较,差异无统计学意义（P>0.05）。DAI（Ⅱ型）整体认知水平与BDNF Val66Met基因多态性、BDNF浓度具有线性回归关系（F=11.417,P<0.001）,其具有一定的相关性（|β|=0.966、0.877;r=0.569、0.579）。 结论BDNF可影响DAI认知功能,其BDNF Val66Met基因多态性可能是影响DAI认知功能的风险因素之一。     

微创经皮钢板内固定技术联合锁定加压钢板治疗胫骨远端骨折

目的：探讨微创经皮钢板内固定（MIPPO）技术联合锁定加压钢板（LCP）治疗胫骨远端骨折疗效。方法回顾性分析该科2006年1月至2012年7月收治的70例胫骨远端骨折患者,均采用MIPPO技术联合LCP治疗。术后临床检查其软组织损伤情况、踝关节活动度、骨折对线情况以及踝关节功能Mazur评分;X射线及CT检查评估骨折愈合情况。随访时间12～24个月。结果62例（88.57%）患者骨折平稳愈合。经踝关节Mazur 评分,优良率为91.43%（64/70）。5例患者（7.14%）出现切口并发症,5例患者（7.14%）骨折延迟愈合,3例患者（4.29%）出现骨折不愈合,5例患者（7.14%）出现明显的轴向对线不良。结论经MIPPO技术结合LCP治疗,大部分患者胫骨远端骨折可获得平稳的愈合并恢复伤前的活动能力,该法是一种有效的治疗方法,但对出现的并发症应引起足够重视。