表达单纯疱疹病毒Ⅱ型糖蛋白D的重组痘苗病毒活疫苗株的建立

我们以前曾报道，表达单纯疱疹病毒Ⅱ型糖蛋白Ｄ（ＨＳＶ－２ｇＤ）的重组痘苗病毒（实验疫苗株）能保护被免疫小鼠抵抗致死量ＨＳＶ－２病毒的攻击。在此工作基础上，严格按人用疫苗研究要求的实验条件，成功地建立了表达ＨＳＶ－２ｇＤ的重组痘苗病毒活疫苗株。首先将经聚合酶链反应（ＰＣＲ）修饰的ＨＳＶ－２ｇＤ基因插入痘苗表达质粒ｐＪＳＢ１１７５，置于痘苗病毒Ｐ７５Ｋ早／晚期启动子控制下。将此重组质粒用Ｌｉｐｏｆｅｃｔｉｎ方法转染已受野型ＴＫ＋痘苗病毒天坛７６１株感染的人胚肺二倍体细胞。经同位素探针（３２Ｐ－ＨＳＶ－２ｇＤ）原位杂交法和３轮蚀斑纯化，筛选出基因组内整合有ＨＳＶ－２ｇＤ基因的重组痘苗病毒。斑点和Ｓｏｕｔｈｅｒｎ杂交证实，ＨＳＶ－２ｇＤ基因已插入痘苗病毒基因组内预期的ＴＫ区段，间接免疫荧光检测显示，重组病毒感染细胞后能有效地表达ＨＳＶ－２ｇＤ蛋白。     

复方中药注射液对小白鼠艾氏腹水癌细胞膜表面的(Na~+-K~+)-ATP酶和微绒毛影响的电镜观察

本实验应用电镜细胞化学和扫描电镜技术,观察到小白鼠艾氏腹水癌细胞在复方中药注射液的连续作用下,膜表面(Na~+-K~+)-ATP酶活性减弱,微绒毛减退等变化,同时观察到该复方中药注射液对癌细胞增殖的抑制作用,抑瘤率可达87%,癌细胞增殖和(Na~+-K~+)-ATP酶活性及微绒毛的多少有平行关系。讨论了该复方中药抑制癌细胞增殖与膜表面(Na~+-K~+)-ATP酶活性和微绒毛变化的关系。     

免疫吸附疗法治疗高脂血症疗效观察

目的 观察低密度脂蛋白(LDL)免疫吸附疗法对高脂血症的治疗效果.方法73例高脂血症患者接受LDL免疫吸附治疗.比较1次治疗前后各项血脂指标的变化.部分病例1个月后复查血脂各项指标.总结LDL免疫吸附疗法的安全性和副作用.结果①所有患者经1次LDL免疫吸附后血甘油三脂、胆固醇和低密度脂蛋白均有明显下降(p<0.01).高密度脂蛋白略有下降(p>0.05).患者临床症状改善.②治疗后1个月复查,血脂各项指标恢复到治疗前水平(p>0.05).③3例药物治疗不能控制的乳糜微粒血症患者经LDL免疫吸附治疗后血脂明显下降,但仍达不到正常范围.其中1例治疗后继续服用原降脂药物,药物疗效较前提高.④30例患者治疗过程出现低血压.结论LDL免疫吸附能有效降低血甘油三脂、胆圃醇和低密度脂蛋白水平,对高密度蛋白无明显影响.1次治疗所能维持血脂于低水平的时间短暂.部分患者临床症状改善.低血压是主要并发症.     

妊高征患者胎盘血循环中血管舒、缩物质水平的改变及意义

目的　通过测定胎盘部位子宫静脉血中一氧化氮 (nitricoxide,NO)及内皮素 (endothelin ,ET)的浓度 ,并与外周血中同类物质的对比 ,了解妊高征时胎盘血管的病变程度及其与外周血中血管活性物质浓度改变的关系。方法　分别于剖宫产手术前及手术中抽取胎盘部位子宫静脉血和外周静脉血 ,应用硝酸根还原酶与Griess反应相结合的方法测定NO ;应用放射免疫分析法测定ET。结果　妊高征组胎盘部位子宫静脉血中NO2 -/NO3 -为 (70 2 6± 12 6 0 ) μmol/l,外周血清NO-2 /NO-3 为 (6 5 5 2± 14 88) μmol/l,二者之间无显著差异。妊高征组外周血浆ET水平为 (5 3 72± 15 2 8)ng/L ,胎盘部位子宫静脉血浆ET水平为 (5 2 80± 14 19)ng/L ,两者之间无显著性差异 (P >0 .0 1)。与正常晚孕组比较 ,妊高征组血中ET、NO-2 /NO-3 水平均显著增高 (P <0 .0 1)。结论　妊高征患者的子宫、胎盘循环系统血管舒、缩物质平衡失调 ,血管内皮系统的功能亦遭到破坏 ;妊高征患者胎盘血循环ET、NO水平升高 ,但与外周血中ET、NO水平的升高无关。     

硝苯啶、硫氮(艹卓)酮对兔实验性动脉粥样硬化症的影响

硝苯啶和硫氮(艹桌)酮不明显影响血清脂蛋白组分水平,但均显著抑制家兔主动脉动脉粥样硬化形成,降低血浆过氧化脂质、血栓烷和主动脉内中膜胆固醇、磷脂及钙含量,升高血浆6-酮-PGF_(1α),使,TXB_2/6-酮-PGF_(1α)趋于平衡。说明钙在血栓烷-前列环素代谢中起重要作用。     

基于EEG模糊相似性的癫痫发作预测

本研究提出基于EEG序列模糊相似性指数方法预测癫痫发作.首先,结合复自相关法和Cao法对EEG序列进行了相空间重构;然后,计算相关积分时用Gaussian函数代替Heavyside函数,克服了Heavyside函数的刚性边界问题,使得计算相似性指数更加准确和可靠;最后,分析大鼠癫痫EEG信号,检测癫痫发作前期状态.分析结果表明模糊相似性指数方法能够比动态相似性指数方法获得更长的预测时间和更低的错误预测率.     

Expression of the CD7 Ligand K-12 in Human Thymic Epithelial Cells: Regulation by IFN-γ

CD7 is an immunoglobulin superfamily molecule expressed on T, NK, and pre-B lymphocytes. Previous studies have demonstrated a role for CD7 in T- and NK-cell activation and cytokine production. Recently, an epithelial cell secreted protein, K12, was identified as a CD7 ligand. Although CD7 is expressed intrathymically, it is not known if K12 is produced in human thymus. To determine roles that K12 might play in the human thymus, we analyzed expression of K12 in human thymocytes, thymic epithelial cells (TE), and thymic fibroblasts. We found that recombinant human K12 bound strongly to soluble hCD7, with a K_eq of 37.6×10^–9M, and this interaction was inhibited by a novel antihuman K12 monoclonal antibody (K12-A1). K12 mRNA was detected by RT–PCR and northern analysis in human TE and thymic fibroblasts, but not in human thymocytes. Expression of K12 in TE cells was upregulated by IFN- . Taken together, these data demonstrated that K12 is produced by human TE cells and thymic fibroblasts, and is regulated in thymus by IFN- . These data suggest a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN- is upregulated such as myasthenia gravis.     

Family-based association study between autism and glutamate receptor 6 gene in Chinese Han trios.

The glutamate pathways are involved in diverse processes such as learning and memory, epilepsy, and they play important roles in neural plasticity, neural development, and neurodegeneration. It has been proposed that autism could be a hypoglutamatergic disorder. Recently, Jamain et al. reported that the glutamate receptor 6 (GluR6 or GRIK2) is in linkage disequilibrium with autism. In the present study, the transmission disequilibrium test (TDT) and the haplotype transmission were performed to analyze the four SNPs (SNP1: rs995640; SNP2: rs2227281; SNP3: rs2227283; SNP4: rs2235076) of GluR6 in 174 Chinese Han parent-offspring trios. The TDT demonstrated that the two SNPs (SNP2 and SNP3) showed preferential transmission (TDT P = 0.032). The global chi(2) test for haplotype transmission also revealed an association between GluR6 and autism (chi(2) = 10.78, df = 3, P = 0.013). Our results suggested that GluR6 is in linkage disequilibrium with autism.     

An oligonucleotide microarray for the detection of vaccinia virus

Vaccinia virus is a member of the orthopoxvirus group, to which also belongs variola virus, one of the most hazardous pathogens known to man. To establish a model system to detect orthopoxviruses, a vaccinia oligonucleotide microarray is designed, produced and tested. Vaccinia virus is used to test the prepared microarrays. The virus DNA samples in different propagation phases are extracted and hybridised with the oligonucleotide microarray. The results showed that the oligonucleotide microarray can detect vaccinia virus with high specificity and sensitivity.     

糖皮质激素受体对大鼠中性粒细胞粘附的影响

本文通过Percoll梯度离心法分离获得大鼠中性粒细胞(PMNS)后,采用PMNS与玻璃珠粘附的模型,通过给予Dex及糖皮质激素受体(GR)阻断剂Mifepristone(RU_(38486))研究大鼠PMNS粘附过程中GR的作用。结果显示,Dex可以明显抑制PMNS的粘附,其作用随着Dex浓度的增大而增强;单纯给予不同浓度的RU_(38486)未发现明显的PMNS粘附增强,说明RU_(38486)本身对离体的PMNS粘附没有明显的作用;若同时给予Dex和RU_(38486),则Dex抑制PMNS粘附的作用逐渐减小,直至完全逆转。该结果强烈提示:糖皮质激素(GC)具有抑制PMNS粘附的作用,其作用是通过GR介导的,当GR被阻断时,这一抑制作用减弱,甚至消失。