The function of apoptosis and protein expression of bcl-2, p53 and C-myc inthe development of gastric cancer

AIM To understand the rule and possible function of apoptosis and protein expression of bcl-2, p53 and C-myc in chronic gastritis, gastric ulcer, non-classic proliferation of gastric mucosa and gastric cancer.METHODS Apoptosis was detected by using in situ terminal labelling (TUNEL). The protein expression ofbcl-2, p53 and C-myc was detected by immunohistochemical method.RESULTS The indexes of apoptosis in chronic active gastritis, gastric ulcer, mild and severe non-classicproliferation of gastric mucosa, early and progressive gastric cancer were 16.8%±12.3%, 24.1%±20.0%,19.3%±16.4%, 15.7%±15.2%, 10.1%±9.1% and 6.3%±6.0%, respectively. The index of progressivegastric cancer was lower than that of early gastric cancer and non-classic proliferation of gastric mucosa(P<0.05). The positive rate of bcl-2 protein was 9.4%, 27.6%, 52.9%, 75.0%, 83.3% and 46.7%,respectively. The positive rate of bcl-2 of early gastric cancer was higher than that of progressive gastriccancer. The positive rates of p53 protein of severe non-classic proliferation, early and progressive gastriccancer were 25.0%, 33.3% and 63.3%, respectively. The positive rate of p53 of progressive gastric cancerwas higher than that of early gastric cancer and non-classic proliferation (P<0.05). In Lauren types, theindex of apoptosis, protein expression rates of bcl-2, p53 and C-myc of intestinal type were 8.3%±7.2%,38.9%, 77.7% and 56.6%, while that of diffuse type were 5.1%±4.9%, 58.3%, 50.0% and 8.3%,respectively. All markers had statistical difference between two types (P<0.05).CONCLUSION Apoptosis was inhibited stepwise in the development of non-classic proliferation of gastricmucosa to early gastric cancer and then to progressive gastric cancer. The high expression of bcl-2, p53 andC-myc was related to the development of gastric cancer, bcl-2 might play an important role in early gastriccancer while p53 and C-myc act mostly in middle and late stage gastric cancer. The Lauren typing of gastriccancer is closely related to the index of apoptosis and expression of bcl-2, p53 and C-myc.     

我国特色数据库建设研究现状

通过对1996-2009年我国特色数据库研究的调查统计分析,总结和评价了近年我国特色数据库领域的研究重点和研究方向,对特色数据库研究的未来趋势进行了预测。     

细胞周期素D1在口腔鳞状细胞癌组织中的表达及意义

目的 研究口腔鳞状细胞癌(OSCC)组织中细胞周期素D1(Cyclin D1)的表达及意义.方法 应用免疫组织化学Envosion法检测50例OSCC患者与10例正常黏膜组织中Cyelin D1蛋白在病理组织切片中的表达水平,并进行统计学分析.结果 在50例OSCC标本中Cyclin D1主要表达在细胞核,也有少量表达...     

加味身痛逐瘀汤联合甲氨蝶呤治疗类风湿关节炎临床研究

目的:观察加味身痛逐瘀汤联合甲氨蝶呤治疗类风湿关节炎的疗效.方法:60例随机分为治疗组和对照组各30例,两组均口服甲氨蝶呤治疗,治疗组加服加味身痛逐瘀汤.结果:治疗后VAS评分治疗组优于对照组(P＜0.05).两组中医证候评分均明显下降(P＜0.05),关节疼痛程度、晨僵、关节屈伸不利评分治疗组优于对照组(P＜0.05...     

Elevated serum Dickkopf-1 is a biomarker for bone erosion in patients with psoriatic arthritis

Background:Psoriatic arthritis (PsA) is an inflammatory arthropathy characterized by psoriasis and bone erosion on radiology. Dickkopf-1 (Dkk-1) is considered to be the main inhibitor of the Wnt signaling pathway and results in reduced osteoblast proliferation. The aim of this study was to investigate the serum level of Dkk-1 and its association with bone erosion in PsA patients.Methods:Serum Dkk-1 levels were measured by enzyme-linked immunosorbent assay (ELISA) in 69 patients with PsA and 60 controls, including 39 rheumatoid arthritis (RA) patients, and 21 healthy controls (HCs). Rheumatoid factor and anti-cyclic citrullinated peptide levels were also determined by ELISA. The association of Dkk-1 level with clinical and laboratory features of PsA was analyzed. Logistic regression analysis was used to analyze the risk factors for bone erosion in PsA.Results:Dkk-1 was elevated in 68.1% (47/69) of the patients with PsA, 46.2% (18/39) of RA patients, and 9.5% (2/21) of HCs. Serum Dkk-1 concentration was significantly higher in PsA patients compared with that in HCs. The level of serum Dkk-1 was correlated with a swollen joint count, and levels of complement components 3 and 4. Elevated Dkk-1 level (odds ratio = 4.440, 95% confidence interval: 1.246–15.817, P = 0.021) was identified as the risk factor for bone erosion in PsA.Conclusions:The serum level of Dkk-1 is abnormally elevated in PsA patients. The elevation of Dkk-1 might be involved in the mechanism of bone erosion in patients with PsA.     

HCMV感染对人神经胶质瘤U251细胞p38MAPK活性的影响

人巨细胞病毒(HCMV)在人群中的感染非常普遍。随着器官移植的广泛开展、艾滋病的传播以及耐药病毒株的出现,对HCMV致病、致畸机制的研究越来越受到重视。MAPK是细胞内的一类丝氨酸／苏氨酸蛋白激酶,它介导的信号转导通路是生物信号引起核反应的重要通路,参与细胞生长、分化、凋亡等多种生理过程,并在细胞恶性转化等病理过程     

2型糖尿病患者血清与尿铜蓝蛋白水平的变化

目的：检测2型糖尿病患者血清与尿铜蓝蛋白（Cp）浓度的变化及探讨临床意义。方法：采用放射免疫法与速率散射免疫浊度法检测57份血清Cp,并采用速率散射免疫浊度法检测110例健康对照组,104例2型糖尿病患者血清Cp,尿Cp与肌酐（Cr）比值及尿白蛋白（Alb）与Cr比值。将2型糖尿病患者分为血糖控制良好组与血糖控制不良组,糖尿病肾病组与无糖尿病肾病组进行分析与比较。结果：放射免疫法与速率散射免疫浊度法检测血清Cp相关性与可比性良好;血清Cp的正常参考上限为542 mg/L（110例健康人的97.5%可信区间）,尿Cp/Cr的正常参考上限为0.892 ng/mmol（110例健康人的97.5%百分位点）;2型糖尿病患者血清Cp值显著高于健康对照组（P＜0.001）,其中血糖控制不良组血清Cp值显著高于血糖控制良好组（P＜0.01）,有糖尿病肾病组尿Cp/Cr显著高于无糖尿病肾病组（P＜0.001）;尿Cp/Cr对糖尿病肾病的诊断敏感性为91.4%,特异性为61.4%,符合率为75.0%。结论：检测血清Cp水平对于了解糖尿病的病情有一定参考价值,联合检测尿Cp/Cr与Alb/Cr对于糖尿病肾病的早期诊断有重要的临床意义。     

聚醚醚酮内植物骨整合改性的研究进展

聚醚醚酮（PEEK）材料因有着诸多优点而在骨缺损修复等医学领域得到越来越多的关注和应用,同时对于克服其生物惰性,提高其骨整合性能的改性研究成为热点。本文通过查阅国内外关于PEEK材料骨整合改性的相关文献,综述了近年来对骨科植入物PEEK材料进行改性以提高其骨整合性能的相关研究现状,并重点从改性原理的角度进行总结。     

克罗恩病的发病机制及针灸治疗进展与思考

本文从遗传、环境、免疫、精神因素等方面简述了克罗恩病的发病机制。同时,介绍了针刺、艾灸不同治疗方法治疗克罗恩病的临床以及实验研究概况,总结了研究中存在的问题,并就针灸治疗克罗恩病的思路与方法提出一些设想。