全文获取类型
收费全文 | 4295篇 |
免费 | 176篇 |
国内免费 | 22篇 |
专业分类
耳鼻咽喉 | 217篇 |
儿科学 | 119篇 |
妇产科学 | 137篇 |
基础医学 | 500篇 |
口腔科学 | 16篇 |
临床医学 | 344篇 |
内科学 | 1192篇 |
皮肤病学 | 72篇 |
神经病学 | 357篇 |
特种医学 | 85篇 |
外科学 | 454篇 |
综合类 | 34篇 |
一般理论 | 2篇 |
预防医学 | 228篇 |
眼科学 | 122篇 |
药学 | 402篇 |
中国医学 | 2篇 |
肿瘤学 | 210篇 |
出版年
2023年 | 19篇 |
2022年 | 105篇 |
2021年 | 249篇 |
2020年 | 70篇 |
2019年 | 89篇 |
2018年 | 127篇 |
2017年 | 95篇 |
2016年 | 84篇 |
2015年 | 95篇 |
2014年 | 140篇 |
2013年 | 217篇 |
2012年 | 325篇 |
2011年 | 377篇 |
2010年 | 179篇 |
2009年 | 133篇 |
2008年 | 263篇 |
2007年 | 293篇 |
2006年 | 303篇 |
2005年 | 306篇 |
2004年 | 283篇 |
2003年 | 243篇 |
2002年 | 224篇 |
2001年 | 24篇 |
2000年 | 20篇 |
1999年 | 16篇 |
1998年 | 27篇 |
1997年 | 15篇 |
1996年 | 14篇 |
1995年 | 8篇 |
1994年 | 14篇 |
1993年 | 11篇 |
1992年 | 9篇 |
1991年 | 10篇 |
1990年 | 6篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 7篇 |
1985年 | 11篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1982年 | 9篇 |
1980年 | 6篇 |
1978年 | 5篇 |
1977年 | 9篇 |
1975年 | 3篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1966年 | 2篇 |
1961年 | 2篇 |
排序方式: 共有4493条查询结果,搜索用时 15 毫秒
991.
992.
993.
994.
995.
996.
Wojciech Jelski Barbara Mroczko Maciej Szmitkowski 《Digestive diseases and sciences》2010,55(10):2953-2957
Background
The activity of total alcohol dehydrogenase (ADH) and class I isoenzymes is significantly higher in colorectal cancer tissue than in healthy mucosa. The activity of these enzymes in cancer cells is probably reflected in the sera and could thus be helpful for diagnosing colorectal cancer. 相似文献997.
998.
Influence of Diabetes Mellitus on Outcome in Patients Over 40 Years of Age With the Long QT Syndrome
999.
Szliszka E Zydowicz G Janoszka B Dobosz C Kowalczyk-Ziomek G Krol W 《International journal of oncology》2011,38(4):941-953
Prostate cancer represents an ideal disease for chemopreventive intervention. Propolis possesses immuno-modulatory, anti-tumour and chemopreventive properties. The tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is an important endogenous anti-cancer agent that induces apoptosis selectively in tumour cells. However, some cancer cells are resistant to TRAIL-mediated apoptosis. Naturally occurring phenolic and polyphenolic compounds sensitize TRAIL-resistant cancer cells and augment the apoptotic activity of TRAIL. The ethanolic extract of Brazilian green propolis (EEP) is rich in phenolic components. Our in vitro results indicate the potential targets in the TRAIL-induced apoptotic pathway for the cancer chemopreventive activity of Brazilian propolis. We examined the cytotoxic and apoptotic effects of Brazilian EEP and its bioactive components in combination with TRAIL on LNCaP prostate cancer cells. The chemical composition of Brazilian green propolis was determined by high performance liquid chromatography-diode array detection. The cytotoxicity was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl-tetrazolium and lactate dehydrogenase assays. Apoptosis was detected using annexin V-FITC by flow cytometry and fluorescence microscopy. The mitochondrial membrane potential (?Ψm) was evaluated using DePsipher staining by fluorescence microscopy. Flow cytometry was used to analyse death receptor (TRAIL-R1 and TRAIL-R2) expression in LNCaP cells. The inhibition of nuclear factor-κB (NF-κB) (p65) activation in cancer cells was confirmed by the ELISA-based TransAM NF-κB kit. The LNCaP cells were shown to be resistant to TRAIL-induced apoptosis. Our study demonstrates that EEP sensitizes TRAIL-resistant prostate cancer cells. The main phenolic components detected in Brazilian green propolis are artepillin C, quercetin, kaempferol and p-coumaric acid. Brazilian propolis and its bioactive components markedly augmented TRAIL-mediated apoptosis and cytotoxicity in prostate cancer cells. Brazilian EEP enhanced the expression of TRAIL-R2 and the activity of NF-κB in LNCaP cells. The co-treatment of prostate cancer cells with 100 ng/ml TRAIL and 50 μg/ml EEP increased the percentage of apoptotic cells to 65.8 ± 1.2% and caused a significant disruption of ?Ψm in LNCaP cells. We show that Brazilian EEP helped cells overcome TRAIL resistance by engaging both intrinsic and extrinsic apoptotic pathways and regulating NF-κB activity. The data demonstrate the important role of Brazilian green propolis and its bioactive compounds in prostate cancer chemoprevention through the enhancement of TRAIL-mediated apoptosis. 相似文献
1000.
Marcin Barczyński Aleksander Konturek Alicja Hubalewska-Dydejczyk Filip Gołkowski Stanisław Cichoń Wojciech Nowak 《World journal of surgery》2010,34(6):1203-1213