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31.
目的:综述人脂肪组织来源干细胞的生物学特性及其在缺血性心脏病中的应用,分析不足,并在此基础上提出未来研究要解决的问题,以期为临床治疗提供依据。资料来源:应用计算机检索Blackwell、Elsevier、Pubmed数据库1980/2007期间脂肪源性干细胞与缺血性心脏病方面的文献,检索词为“bone mesenchymal stem cells,adipose derived stemcells,cardiomyocytes,ischemic heart disease”等。应用计算机检索中国期刊全文数据库1980/2007期间相关文献,检索词为“骨髓间充质干细胞,脂肪组织来源的干细胞,心肌细胞,缺血性心脏病”等。并手工查阅相关书籍。资料选择:对资料进行初步选择:①脂肪组织来源干细胞的生物学特性。②脂肪组织来源干细胞治疗缺血性心脏病。排除重复文献。资料提炼:共搜集到相关文章57篇,删除内容重复及与本文主题关系较远的文章,剩余41篇作为综述参考。资料综合:脂肪组织来源干细胞与同样起源于中胚层的骨髓基质细胞不仅具有非常相似的生物学特性,而且在细胞表面标志谱的表达方面也非常相近。并且脂肪组织来源广泛,取材方便,可获得的基质细胞数量大,易于培养扩增。有研究发现,脂肪组织来源干细胞体外培养不需要任何诱导便能分化成具有自律性的心肌细胞,使得脂肪组织来源干细胞治疗缺血性心脏病成为可能。结论:脂肪组织来源干细胞在取材和增殖方面较骨髓间充质干细胞有优势;脂肪组织来源干细胞能较好的诱导为心肌细胞,将为缺血性心脏病的治疗提供更广阔的前景。 相似文献
32.
Hinsch E; Ponce AA; Hagele W; Hedrich F; Muller-Schlosser F; Schill WB; Hinsch KD 《Human reproduction (Oxford, England)》1997,12(8):1673-1681
Binding of mammalian spermatozoa to the zona pellucida and the induction of
the acrosome reaction are prerequisites for successful oocyte
fertilization. It has been postulated that xenobiotics that are released in
the environment as well as exposure to pharmaceutical medications may be
associated with reproductive problems in men and wildlife. Examining
physiological and non-physiological effects of particular compounds on
sperm functions requires high quality in-vitro test systems. We established
a reliable combined in-vitro test system with bovine gametes and evaluated
if aliquots of pooled post-thaw spermatozoa are suitable for examining
essential sperm functions. Using cryopreserved semen, the PSA-FITC/Hoechst
33258 staining procedure was applicable to evaluate the acrosomal status
and cell viability. In the bovine hemizona assay, hemizona indices revealed
no differences between cryopreserved and fresh semen. Treatment of
post-thaw bovine spermatozoa with progesterone (1 microM or bovine
follicular fluid (20%) induced the acrosome reaction from 12% (untreated
spermatozoa) to 25% (P < 0.001) and to 22% [corrected] (P < 0.01),
respectively. Incubation of both compounds (1 microM progesterone and 20%
follicular fluid) raised the percentage of acrosome-reacted spermatozoa to
30% (P < 0001). Our results demonstrate that cryopreserved semen can be
integrated into an in-vitro screening model for reproductive toxicology
testing. Pooled, cryopreserved bovine spermatozoa will thus permit
reproducible experiments for clinical and basic science purposes and may
also be applicable for the human system.
相似文献
33.
34.
TR Fricke BA Holden DA Wilson G Schlenther KS Naidoo S Resnikoff KD Frick 《Bulletin of the World Health Organization》2012,90(10):728-738
Objective
To estimate the global cost of establishing and operating the educational and refractive care facilities required to provide care to all individuals who currently have vision impairment resulting from uncorrected refractive error (URE).Methods
The global cost of correcting URE was estimated using data on the population, the prevalence of URE and the number of existing refractive care practitioners in individual countries, the cost of establishing and operating educational programmes for practitioners and the cost of establishing and operating refractive care facilities. The assumptions made ensured that costs were not underestimated and an upper limit to the costs was derived using the most expensive extreme for each assumption.Findings
There were an estimated 158 million cases of distance vision impairment and 544 million cases of near vision impairment caused by URE worldwide in 2007. Approximately 47 000 additional full-time functional clinical refractionists and 18 000 ophthalmic dispensers would be required to provide refractive care services for these individuals. The global cost of educating the additional personnel and of establishing, maintaining and operating the refractive care facilities needed was estimated to be around 20 000 million United States dollars (US$) and the upper-limit cost was US$ 28 000 million. The estimated loss in global gross domestic product due to distance vision impairment caused by URE was US$ 202 000 million annually.Conclusion
The cost of establishing and operating the educational and refractive care facilities required to deal with vision impairment resulting from URE was a small proportion of the global loss in productivity associated with that vision impairment. 相似文献35.
Regression of experimental Burkitt's lymphoma induced by Epstein-Barr virus-immortalized human B cells 总被引:3,自引:1,他引:3
Tosato G; Sgadari C; Taga K; Jones KD; Pike SE; Rosenberg A; Sechler JM; Magrath IT; Love LA; Bhatia K 《Blood》1994,83(3):776-784
Epstein-Barr virus (EBV)-immortalized human B cells survive only transiently when injected subcutaneously into athymic mice, whereas Burkitt's lymphoma cells give rise to progressively growing subcutaneous tumors. In this study, we tested whether these Burkitt's tumors could be induced to regress via a bystander effect induced by EBV-immortalized B cells. Simultaneous inoculation of EBV-immortalized B cells and Burkitt's lymphoma cells in the same subcutaneous site resulted in tumors that regressed with necrosis and scarring. Similarly, simultaneous inoculation of EBV-immortalized B cells and Burkitt's lymphoma cells in separate subcutaneous sites resulted in regression of a proportion of the Burkitt's tumors. Furthermore, most of the established human Burkitt's tumors regressed with necrosis and scarring after intratumor inoculations with EBV-immortalized B cells. The EBV-immortalized B cells continued to exert this antitumor effect even when killed with irradiation. The experimental approach to Burkitt's lymphoma treatment described here exploits the ability of athymic mice to reject EBV-immortalized B cells to target an effective antitumor response to malignant cells normally incapable of eliciting it. 相似文献
36.
很久以来都认为遗传成分参与2型糖尿病(T2DM)的发病,但是有关遗传学上的发现一直进展缓慢,这是由于遗传成分的复杂性。有关糖尿病相关的各种表型的研究的大量资料提示,所谓的“T2DM”可能是许多疾病的统称,由于它们具有通常相互重叠的多种基本发病机制。因此,对曾抱有期望的T2DM的遗传学基础的寻求已经证明是很艰难的。 相似文献
37.
Werber D Fruth A Liesegang A Littmann M Buchholz U Prager R Karch H Breuer T Tschäpe H Ammon A 《The Journal of infectious diseases》2002,186(3):419-422
In the spring of 2000, a cluster of indistinguishable Shiga toxin-producing Escherichia coli (STEC) O26:H11 was identified in Germany by molecular subtyping surveillance. An investigation was prompted to identify a common source of exposure. A case subject was defined as a person having a polymerase chain reaction-confirmed STEC O26 infection between March and April 2000, irrespective of clinical signs, and whose isolate was indistinguishable from the index strain by use of pulsed-field gel electrophoresis. Eleven case subjects were found in 5 institutions that were supplied by 4 kitchens located in 3 states. The median age was 2 years (range, 2-31 years). No bloody diarrhea was reported, and 5 persons remained asymptomatic. Comparison of invoices revealed a certain type of beef ("Seemerrolle") as possible source of infection. This is, to our knowledge, the first multistate outbreak associated with a non-O157 STEC detected by laboratory-based surveillance. Molecular subtyping was pivotal, as disease occurrence was sporadic or family-related. 相似文献
38.
This study compared end-systolic pressure-volume relationship (P/V) versus wall stress index (WS) as a tool for the evaluation of ventricular contractile state during upright isometric exercise. Both indices of contractility exhibit significant (p < 0.001) increase during exercise compared to resting values. The correlation coefficients for the two indices of contractility were r = -0.45 for exercise and r = -0.70 for rest. Both were significant at the p < 0.01 level. The correlation of the difference scores between rest and exercise as computed by both indices was low (-0.20) and insignificant. These data suggest that changes in contractility from rest to exercise as measured by one index do not reflect the pattern of individual differences that are measured by means of the other index. However, from a reliability point of view, it seems appropriate to prefer the use of P/V index over the WS index in order to determine contractile state. 相似文献
39.
H D Itskovitz J L Werber A M Sheridan T F Brewer C T Stier 《Journal of hypertension》1989,7(4):311-315
Serial measurements of blood pressure, body weight, food and water intake, and salt and water excretion were compared in two groups of spontaneously hypertensive rats (SHR) over a 12-day period: control SHR (n = 11) and a group (n = 9) which received supplementary 5-hydroxytryptophan (5-HTP; 2 mg/ml) in its drinking water. During the final 4 days of study, both groups received additional oral carbidopa (50 mg/kg twice a day) to inhibit peripheral, but not brain aromatic L-amino-acid decarboxylase (LAAD), an enzyme necessary to the formation of 5-hydroxytryptamine (5-HT, serotonin) from 5-HTP. 5-Hydroxytryptophan increased urinary 5-HT and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) markedly; following carbidopa, urinary 5-HT, and to a lesser degree urinary 5-HIAA, decreased, whereas brain 5-HT and 5-HIAA increased. Spontaneously hypertensive rats treated with 5-HTP plus carbidopa had significantly lower blood pressure levels, lower pulse rates, reductions in food and water intake, salt and water excretion, and a loss of body weight, when compared with the control SHR. These data indicate that enhanced brain formation of 5-HT can give rise to metabolic and circulatory responses with a resultant lowering of blood pressure. 相似文献