Reversal of multidrug resistance and inhibition of phosphorylation of AKT in human ovarian cancer cell line by wild-type PTEN gene

Summary The reversing effect of wild-type PTEN gene on resistance of C13K cells to cisplatin and its inhibitory effect on the phosphorylation of protein kinase B (AKT) were studied. The expression of PTEN mRNA and protein in OV2008 cells and C13K cells were semi-quantitatively detected by using RT-PCR and Western blotting. Recombinant eukaryotic expression plasmid containing human wild-type PTEN gene was transfected into C13K cells by lipofectamine2000. The expression of PTEN mRNA was monitored by RT-PCR and the expression of PTEN, Akt, p-Akt protein were analyzed by Western blotting in PTEN-transfected and non-transfected C13K cells. Proliferation and chemosensitivity of cells to DDP were measured by MTT, and cell apoptosis was detected by flow cytometry after treatment with cisplatin. The expression of PTEN mRNA and protein in OV2008 cells were significantly higher than those in C13K cells. After transfection with PTEN gene for 48 h, the expression of PTEN mRNA and protein in C13K cells were 2.04 ± 0.10, 0.94 ± 0.04 respectively and the expression of p-Akt protein (0.94 ± 0.07) was lower than those in control groups (1.68 ± 0.14, 1.66 ± 0.10) (P < 0.05). The IC₅₀ of DDP to C13K cells transfected with PTEN (7.2 ± 0.3 μmol/L) was obviously lower than those of empty-vector transfected cells and non-transfected cells (12.7 ± 0.4 μmol/l, 13.0 ± 0.3 μmol/L) (P<0.05). The apopototis ratio of wild-type PTEN-transfected, empty vector transfected and non-transfected C13K cells were (41.65 ± 0.87)%, (18.61 ± 0.70)% and (15.28 ± 0.80)% respectively, and the difference was statistically significant (P<0.05). PTEN gene plays an important role in ovarian cancer multidrug resistance. Transfection of PTEN could increase the expression of PTEN and restore drug sensitivity to cisplatin in human ovarian cancer cell line C13K with multidrug-resistance by decreasing the expression of p-Akt. This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30571950) and National Key Basic Research Program Foundation (NO.2002CB513107).     

Myocardial injury was associated with neurological sequelae of acute carbon monoxide poisoning in Taiwan

Background

Carbon monoxide (CO) poisoning has recently become a serious health problem in some Asian countries, including Taiwan. The aims of this study are to evaluate the changing trend of CO poisoning and to demonstrate the association between myocardial injury and neurological sequelae of CO poisoning in Taiwan between 1990 and 2011.

全息影像术中导航在泌尿外科腹腔镜和机器人手术中的应用价值

目的探讨全息影像术中导航在泌尿外科腹腔镜和机器人手术中的应用价值。方法回顾性分析2019年1—12月北京和睦家医院等19家医疗中心收治的86例患者的病例资料,临床诊断包括肾肿瘤78例,膀胱癌2例,肾上腺肿瘤2例,肾囊肿1例,前列腺癌1例,汗腺癌淋巴结转移1例,根治性膀胱切除术后盆腔转移瘤1例。其中,32例接受标准腹腔镜手术,分别为肾部分切除术(LPN)27例,根治性前列腺切除术1例,根治性膀胱切除术2例,肾上腺肿瘤切除术2例;54例接受达芬奇机器人手术,分别为肾部分切除术(RAPN)51例,腹膜后淋巴结清扫术1例,双侧肾囊肿去顶减压术1例,盆腔转移肿瘤切除术1例。两种术式的肾部分切除术患者中41例有可统计的临床数据,其中RAPN 23例,LPN 18例。男26例,女15例;中位年龄53.5(24~76)岁;中位R.E.N.A.L评分7.8(4~11)分。专业工程师根据86例术前增强CT检查图像和诊断报告重建全息影像。术前,全息影像可帮助术者对供应肿瘤或手术切除部位的动静脉血管、淋巴结和神经的立体空间结构及相互关系有更直观的认识,协助术前手术规划。在术中将全息影像与腹腔镜监视器中的术野图像进行实时追踪和融合,实现术中导航。结果本组86例手术均顺利完成。术中通过人工调整全息影像,可提示重要血管如肾动脉和肾静脉在体内的投影位置,帮助术者定位血管、淋巴结和其他重要解剖结构的位置,便于精准分离。本研究采用标准腹腔镜手术和达芬奇机器人手术的肾部分切除术患者中共41例有可统计的临床数据。41例中位手术时间140(50~225)min,其中RAPN为140(50~215)min,LPN为160(80~225)min;中位热缺血时间23(14~60)min,其中RAPN为21(17~40)min,LPN为25(14~60)min;中位出血量80(5~1200)ml,其中RAPN为150(30~1200)ml,LPN为50(5~1200)ml。术中无周围重要脏器损伤。2例LPN患者出现ClavienⅡ级并发症,1例出血1200 ml给予输血,另一例血肿未予处理,自行吸收。此2例分别为前、后肾门肿瘤,R.E.N.A.L评分均为11分。结论全息影像术中导航可协助术者在腹腔镜和机器人手术中对重要的解剖结构进行定位、识别,在减少术中组织和器官损伤、降低手术并发症和提高手术成功率方面有重要的应用价值。     

冷却塔轴流风机的维护与检修及播水系统的改造

从减速机、联轴器、扇叶与风筒的检查维护以及润滑油系统、振动和腐蚀的监测等六个方面，介绍了冷却塔风机维护与检修的方法，同时介绍了对冷却塔的播水系统的改进。     

电力电缆的预防性试验

主要叙述了电缆做预防性试验及其试验项目、方法和要求，以及需注意的问题。     

前列腺癌组织中TMPRSS2基因与ETS家族基因的多种融合亚型及其意义

目的 了解前列腺癌组织标本中跨膜丝氨酸蛋白酶2(TMPRSS2)基因与ETS转录因子家族成员ETS相关基因(ERG)、ETS变异体1(ETV1)及ETS变异体4(ETV4)基因之间的融合情况及意义.方法 采用巢式逆转录聚合酶链反应(RT-PCR)琼脂糖凝胶电泳法检测32例前列腺癌患者及34例前列腺良性增生患者前列腺组织中TMPRSS2基因与ETS家族基因融合的TMPRSS2/ERG、TMPRSS2/ETV1、TMPRSS2/ETV4转录体;琼脂糖凝胶电泳阳性者,纯化PCR产物进行直接测序,用BLAST在线软件比对确定融合位点;分析融合基因与Gleason分级关系.结果 在32例前列腺癌患者组织标本中,检测到TMPRSS2/ERG融合基因17例(53.1%),含5种不同融合基因亚型,其中1种为新发现融合基因亚型(GenBank登录号:EU090248),单一标本中可检测到1种以上TMPRSS2/ERG融合基因亚型;检测到TMPRSS2/ETV1融合基因2例(6.3%),为新发现融合基因亚型(GenBank 登录号:EU090249);未检到TMPRSS2/ETV4融合基因型.34例前列腺良性增生组织标本中均未检测到TMPRSS2/ERG、TMPRSS2/ETV1和TMPRSS2/ETV4融合基因型.按Gleason评分值分为中分化与低分化的两组前列腺癌组织标本之间融合基因阳性率差异无统计学意义(P=0.169).结论 前列腺癌组织中存在TMPRSS2/ERG和TMPRSS2/ETV1融合基因及多种亚型;前列腺癌融合基因的发现有望为前列腺癌的发病机制研究提供新的思路.     

推拿松肌及整骨手法相结合治疗腰椎间盘突出症77例

[目的]总结推拿松肌及整骨手法相结合治疗腰椎间盘突出症的临床疗效.[方法]运用松肌及整骨手法相结合的非手术方法治疗腰椎间盘突出症77例.[结果]77例腰椎间盘突出症患者中,痊愈57例,显效16例,无效4例,总有效率达94.81%.[结论]以推拿松肌及整骨手法相结合治疗腰椎间盘突出症,疗效满意.     

多层螺旋CT重建技术在颌面部骨折的临床应用

目的探讨多层螺旋CT多平面重建（MPR）、容积重现（vR）和最大密度投影重建（MIV）在颌面部骨折中的临床应用价值。方法对96例颌面部创伤患者进行多层螺旋CT横断位容积扫描，常规层厚2mm，重建间隔1mm，螺距0．9mm,原始图像传输至工作站进行MPR、VR、MIP三维重建。结果三维重建影像显示骨折范围、位置、类型、骨折线走行方向及碎骨片移位等信息方面较轴位扫描图像有显著优势。结论多层螺旋CT三维重建技术的应用对颌面部骨折显示良好，有利于临床制定治疗方案。     

Effects of armepavine against hepatic fibrosis induced by thioacetamide in rats

The aim of this study was to investigate if armepavine (Arm, C₁₉H₂₃O₃N) could exert inhibitory effects against hepatic fibrosis in rats. A cell line of rat hepatic stellate cells (HSC‐T6) was stimulated with tumour necrosis factor‐α (TNF‐α) to evaluate the inhibitory effects of Arm. Rats were injected with thioacetamide (TAA; 300 mg/kg, intraperitoneally) thrice a week for 4 weeks to induce hepatic fibrosis, with Arm (3 or 10 mg/kg) given by gavage twice a day. Liver sections were taken for western blotting, fibrosis scoring and immunofluorescence staining. Arm (1–10 µm ) concentration‐dependently attenuated TNF‐α‐stimulated: (i) protein expressions of α‐smooth muscle actin (α‐SMA), collagen type I and angiopoietin‐1; (ii) H₂O₂ production; and (iii) NF‐κB, JunD and C/EBPß (cytidine‐cytidine‐adenosine‐adenosine‐thymidine (CCAAT)/enhancer binding protein‐ß (EBPß)) nuclear translocations in HSC‐T6 cells. In vivo Arm treatment significantly reduced plasma aspartate transaminase and alanine transaminase levels, hepatic α‐SMA expression and collagen contents, and fibrosis scores of TAA‐injected rats. Moreover, Arm treatment decreased α‐SMA‐ and NF‐κB‐positive cells in immunohistochemical staining, and mRNA expression levels of IL‐6, TGF‐ß1, TIMP‐1, col1α2, iNOS and ICAM‐1 genes, but up‐regulated the metallothionein gene in the livers of TAA‐injected rats. Our results indicated that Arm exerted both in vitro and in vivo antifibrotic effects in rats, with inhibition of NF‐κB, JunD and C/EBPß pathways. Copyright © 2011 John Wiley & Sons, Ltd.