全文获取类型
收费全文 | 6507篇 |
免费 | 591篇 |
国内免费 | 310篇 |
专业分类
耳鼻咽喉 | 36篇 |
儿科学 | 121篇 |
妇产科学 | 139篇 |
基础医学 | 881篇 |
口腔科学 | 141篇 |
临床医学 | 824篇 |
内科学 | 1120篇 |
皮肤病学 | 85篇 |
神经病学 | 402篇 |
特种医学 | 221篇 |
外科学 | 920篇 |
综合类 | 765篇 |
现状与发展 | 2篇 |
一般理论 | 3篇 |
预防医学 | 364篇 |
眼科学 | 145篇 |
药学 | 542篇 |
1篇 | |
中国医学 | 212篇 |
肿瘤学 | 484篇 |
出版年
2024年 | 17篇 |
2023年 | 100篇 |
2022年 | 218篇 |
2021年 | 279篇 |
2020年 | 206篇 |
2019年 | 188篇 |
2018年 | 255篇 |
2017年 | 197篇 |
2016年 | 204篇 |
2015年 | 256篇 |
2014年 | 338篇 |
2013年 | 299篇 |
2012年 | 500篇 |
2011年 | 533篇 |
2010年 | 305篇 |
2009年 | 277篇 |
2008年 | 348篇 |
2007年 | 337篇 |
2006年 | 307篇 |
2005年 | 305篇 |
2004年 | 258篇 |
2003年 | 219篇 |
2002年 | 182篇 |
2001年 | 141篇 |
2000年 | 142篇 |
1999年 | 152篇 |
1998年 | 103篇 |
1997年 | 80篇 |
1996年 | 74篇 |
1995年 | 70篇 |
1994年 | 54篇 |
1993年 | 36篇 |
1992年 | 48篇 |
1991年 | 39篇 |
1990年 | 47篇 |
1989年 | 32篇 |
1988年 | 26篇 |
1987年 | 27篇 |
1986年 | 28篇 |
1985年 | 18篇 |
1984年 | 17篇 |
1983年 | 17篇 |
1982年 | 9篇 |
1981年 | 13篇 |
1980年 | 8篇 |
1979年 | 16篇 |
1977年 | 12篇 |
1973年 | 10篇 |
1970年 | 10篇 |
1966年 | 9篇 |
排序方式: 共有7408条查询结果,搜索用时 0 毫秒
71.
72.
Libin Zhou Hualong Fang Min Yin Huimin Long Guobin Weng 《Journal of clinical laboratory analysis》2022,36(6)
BackgroundClear cell renal cell carcinoma (ccRCC) is the most common malignant tumor of the kidney and is characterized by poor prognosis. We sought to build an immune‐related prognostic signature and investigate its relationship with immunotherapy response in ccRCC.MethodsImmune‐related genes were identified by ssGSEA and WGCNA. The prognostic signature was conducted via univariate, least absolute shrinkage and selection operator, and multivariable Cox regression analyses. Kaplan‐Meier analysis, PCA, t‐SNE, and ROC were used to evaluate the risk model.ResultsA total of 119 immune‐related genes associated with prognosis were screened out. Six immune‐related genes (CSF1, CD5L, AIM2, TIMP3, IRF6, and HHLA2) were applied to construct a prognostic signature for KIRC. Kaplan–Meier analysis showed that patients in high‐risk group had a poorer survival outcome than in low‐risk group. The 1‐, 3‐ and 5‐year AUC of the prognostic signature was 0.754, 0.715, and 0.739, respectively. Univariate and multivariate Cox regression models demonstrated that the risk signature was an independent prognostic factor for KIRC survival. GSEA analysis suggested that the high‐risk group was concentrated on immune‐related pathways. The high‐risk group with more regulatory T‐cell infiltration showed a higher expression of immune negative regulation genes. The risk score had positively relationship with TIDE score and negatively with the response of immunotherapy. The IC50 values of axitinib, sunitinib, sorafenib, and temsirolimus were lower in the high‐risk group.ConclusionOur study defined a robust signature that may be promising for predicting clinical outcomes and immunotherapy and targeted therapy response in ccRCC patients. 相似文献
73.
Jinghui Peng Shengbin Pei Yangyang Cui Yiqin Xia Yue Huang Xiaowei Wu Mingjie Zheng Miaomiao Weng Xu Han Hongtao Fu Lili Yang Wenbin Zhou Ziyi Fu Shui Wang Hui Xie 《Oncology Letters》2022,24(2)
In patients with triple-negative breast cancer (TNBC), high tumour mutation burden and aberrant oncogene expression profiles are some of the causes of poor prognosis. Therefore, it is necessary to identify aberrantly expressed oncogenes, since they have the potential to serve as therapeutic targets. Transient receptor potential channel 5 opposite strand (TRPC5OS) has been previously shown to function as a novel tumour inducer. However, the underlying mechanism of TRPC5OS function in TNBC remain to be elucidated. Therefore, in the present study TRPC5OS expression was first measured in tissue samples of patients with TNBC and a panel of breast cancer cell lines (ZR-75-1, MDA-MB-453, SK-BR-3, JIMT-1, BT474 and HCC1937) by using qRT-PCR and Western blotting. Subsequently, the possible effects of TRPC5OS on MDA-MB-231 cells proliferation were determined using Cell Counting Kit-8 and 5-Ethynyl-2′-deoxyuridine assays after Lentiviral transfection of MDA-MB-231. In addition, potential interaction partners of TRPC5OS were explored using liquid chromatography-mass spectrometry (LC-MS)/MS. Gene expression patterns following TRPC5OS overexpression were also detected in MDA-MB-231 cells by using High-throughput sequencing. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis were then used to systematically verify the potential interactions among the TRPC5OS-regulated genes. The potential relationship between TRPC5OS-interacting proteins and gene expression patterns were studied using Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) analysis. TRPC5OS expression was found to be significantly higher in TNBC tumour tissues and breast cancer cell lines compared with luminal tumour tissues and ZR-75-1. In addition, the overexpression of TRPC5OS significantly increased cell proliferation. High-throughput sequencing results revealed that 5,256 genes exhibited differential expression following TRPC5OS overexpression, including 3,269 upregulated genes and 1,987 downregulated genes. GO analysis results indicated that the functions of these differentially expressed genes were enriched in the categories of ‘cell division’ and ‘cell proliferation’ regulation. KEGG analysis showed that the TRPC5OS-regulated genes were associated with processes of ‘homologous recombination’ and ‘TNF signalling pathways’. Subsequently, 17 TRPC5OS-interacting proteins were found using LC-MS/MS and STRING analysis. The most important protein among interacting proteins was ENO1 which was associated with glycolysis and regulated proliferation of cancer. In summary, data from the present study suggest that TRPC5OS overexpression can increase TNBC cell proliferation and ENO1 may be a potential target protein mediated by TRPC5OS. Therefore, TRPC5OS may serve as a novel therapeutic target for TNBC. 相似文献
74.
<正>Colorectal cancer(CRC) is the third most common cancer worldwide and the second most common in tumor-related mortality by Global Cancer Statistics 2020(1). Recent data show that the incidence and mortality of CRC in China are increasing(2-4), with the number of new cases and deaths reaching 607,900 and 261,777 in 2019. 相似文献
75.
76.
<正> 自1967年Feignbaum首先应用超声心动图测算心搏量以来,发展迅速,目前已广泛用于心脏功能和结构的测定。Morganroth等1975年发表题为“有训练运动员运动性左室肥大”。我国白洁心等于1979年报道了运动员超声心动图的测量分析。近几年来,国内关于运动员的超声心动图已先后发表了二十多篇文章,都是属于一次性的测定,在作横向比较时,由于仪 相似文献
77.
78.
79.
为探讨新合成化合物XW630对成骨细胞的作用,采用噻唑蓝比色法(MTT)法和碱性磷酸酶(ALP)测定法观察了XW630对体外培养的成熟大鼠头盖骨成骨细胞的增殖和ALP的表达,并用抗酒石酸磷酸酶染色法观察XW630对体外培养的破骨细胞形成的影响。结果显示:XW630有刺激成骨细胞增殖,提高ALP活性及抑制甲状旁腺素促进破骨细胞形成的作用,从而表明XW630有可能成为治疗骨质疏松症的有效药物。 相似文献
80.
观察和比较美满霉素、四环素、灭滴灵、青霉胺、氯喹、布洛芬、头孢拉定、异搏定和三氟拉嗪对中性粒细胞(PMN)产生的ROS和非细胞体系产生的O和·OH的影响。应用化学发光法(CL)测定人全血吞噬细胞在受调理的酵母多糖诱导下产生的活性氧(ROS)、黄嘌呤-黄嘌呤氧化酶体系产生的O和VitC-CuSO4-酵母多糖-H2O2体系产生的·OH。结果:所有9种药物都具有抑制或清除中性粒细胞产生的ROS;美满霉素、四环素、三氟拉嗪具有清除O作用;青霉胺和美满霉素则有清除·OH的作用。结论:美满霉素是9种药物中较为安全、有效的清除皮肤疾患中ROS的首选药物。 相似文献