人碱性成纤维细胞生长因子基因在大肠杆菌中的克隆与表达

应用DNA重组技术将编码人碱性成纤维细胞生长因子（bbFGF）的基因克隆至原核高效表达质粒pBV_(221)的启动子下游。SDS-SAGE、ELISA和NTT活性监测结果表明:该重组质粒pBV-hbFGF在大肠杆菌DH5α中,经42℃诱导后,可表达出有较高生物活性的hbFGF。     

煤烟型大气污染对儿童肺功能的影响

为观察煤烟型大气污染对儿童健康的影响 ,选择了太原市 3个污染程度不同地区的 45 0名儿童进行肺功能测试和问卷调查。研究结果显示 ,儿童呼吸功能表现为重度污染区 <中度污染区 <相对清洁区 ;肺通气功能阻塞性异常率表现为重度污染区 >中度污染区 >相对清洁区 ;Logistic回归分析发现肺通气功能阻塞性异常率与小学生家庭的取暖方式、厨房和卧室是否分开以及污染物的水平有关 ;多元线性回归分析显示重度污染区儿童的FVC、FEF5 0与相对清洁区相比分别减少了 65 80ml、119 2 7ml,中度污染区儿童的FVC、FEF5 0与相对清洁区相比分别减少了 5 7 2 8ml和 114 2 9ml;Ln(SO2 )每升高一个单位 ,FVC和FEF5 0分别减少69 10ml和 119 79ml,Ln(PM1 0 )每升高一个单位 ,FVC、FEF5 0等分别减少了 193 5 0ml和 171 69ml。结果提示 ,太原市煤烟型大气污染对儿童呼吸系统产生了危害。     

应用PEG沉淀试验测定囊虫病人血清中抗体的初步研究

用适宜浓度的PEG将囊虫病人血清浓缩,达到提高血清中特异性抗体浓度的作用,从而提高检测的敏感性。该法用于囊虫病的诊断,阳性附合率83.33%.特异性较理想。     

Lung cancer and prior tuberculosis infection in Shanghai

In a population-based case-control study of lung cancer in Shanghai involving interviews during 1984-86 with 1,405 cancer patients and 1,495 controls, a significant 50% elevation in the risk of lung cancer, adjusted for cigarette smoking, was observed among persons who had a history of tuberculosis. Among those diagnosed with tuberculosis within the past 20 years, the risk exceeded 2.5-fold. In males the lung cancers tended to occur on the same side as the previous tuberculosis infection. For both sexes, the effect of recent tuberculosis was most apparent for adenocarcinoma and peripheral tumours. No relationship was found between lung cancer risk and the type of tuberculosis therapy, including use of isoniazid. The findings suggest that tuberculosis may predispose to lung cancer, with the association most apparent among recent survivors of the infection.     

Transtemporal supralabyrinthine approach]

Transtemporal supralabyrinthine approach is a modified middle cranial fossa approach. It offers all the advantages of a middle cranial fossa procedure and avoids its disadvantages, mainly the extensive temporal lobe retraction and frightening complications. The principle of the approach is to gain sufficient access toward the internal auditory canal by removing bone from the roof of the petrous pyramid rather than by elevating the middle fossa dura away from it. Fifteen patients underwent this approach for decompression of paralysed facial nerve resulted from temporal bone fracture, Bell's palsy and herpes zoster oticus; for removal of facial neuromas and primary cholesteatomas in temporal bone and for sectioning of great superficial petrous nerve. Preliminary study showed good results.     

经胸小切口在食管癌切除手术中的应用

目的探讨经胸小切口治疗食管癌手术的地位和可行性。方法对132例不同分期的食管癌行经胸小切口根治性手术,全部采用颈部吻合。结果切口平均长度13.0±2.0 cm,开关胸时间、开胸时出血量、术后伤口疼痛、呼吸功能受限及患肢活动等方面有显著差异,在手术时间、淋巴结清扫、术中出血及术后胸腔引流和常规手术无差异。结论经胸小切口食管癌根治手术安全、可行,并且有创伤小、恢复快等优点,便于推广应用。     

Detection of Human Heart Rate in Exercises State

作者介绍了一种动态心率检测电路，它能准确、可靠地检出在运动状态下的人体心率。电路采用两种检测技术，一种为双时值ＱＲＳ波检测法，另一种为光电脉搏检测法。     

糖尿病患者第3腰椎骨密度测量的意义

糖尿病（ＤＭ）患者继发骨质疏松较多见，为探讨早期诊断，对３０例非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者和４０例正常对照者，进行血清钙（Ｃａ）、磷（Ｐ）、碱性磷酸酶（ＡＫＰ）的测定，拍照胸部、腰椎和骨盆Ｘ线片，并分别经ＣＴ进行第３腰椎骨密度（ＢＭＤ）测量。结果，ＤＭ组ＢＭＤ均值为１２７．１±２５．７７ｍｇ／ｃｍ３，对照组ＢＭＤ均值为１８９．１１±７１．６８ｍｇ／ｃｍ３，两组比较有非常显著差异（Ｐ＜０．０１），提示第３腰椎骨密度测量对ＤＭ患者骨质疏松的早期诊断有一定价值。