Opinions on the provision of dental care in Iowa nursing homes

Using a stratified random sample, a questionnaire was mailed to 400 practicing dentists and 200 directors of nursing (DONs). Response rates were 58.3% for dentists and 50% for DONs. Dentists were representative of Iowa dentists, with 85% male, mean age 49.1 years, and 22.4 years in practice. All DONs were female, with mean age of 44.9 years. Of the participating dentists, 86% had provided dental care for nursing home residents, but the majority of care was completed in dental offices. Three‐quarters of dentists were somewhat/not interested in nursing home dentistry. Dentists and DONs held common perceptions of the most frequent problems related to care provision at nursing homes: low financial reimbursement, especially for Medicaid patients; no portable dental equipment; no suitable area for dentistry; dentist's preference to treat patients at their dental practice; and transportation of residents to a dental practice. Dentists and DONs had some differing perceptions about oral health care (p < .01). Minimal dental care was provided on‐site at Iowa nursing homes.     

红外额式体温计在新生儿体温测量中的应用

目的比较红外额式体温计与传统玻璃水银体温计测量新生儿体温值的差异，研究红外额式体温计应用于新生儿体温测量的准确性及稳定性。方法对广东省妇幼保健院新生儿重症监护室（NICU）住院的202例不同保暖环境下即婴儿床、暖箱、辐射抢救台上的新生儿分别应用红外额式体温计测量前额温度和玻璃水银体温计测量腋下温度，每例新生儿均应用两种方法测量体温，采用自身对照的方法比较两种测量方法的测温效果及相关性。结果红外额式体温计与传统玻璃水银体温计所测得新生儿体温值比较差异无显著性（P〉0．05），且二者呈直线正相关。婴儿床、暖箱和辐射抢救台3种不同保暖环境下额温与腋温比较差异无显著性（P〉0．05）。结论红外额式测温计具有很好的准确性，适用于NICU新生儿。     

Prevalence of SFTSV among Asian House Shrews and Rodents,China, January–August 2013

To evaluate the role of small mammals as hosts of severe fever with thrombocytopenia syndrome virus (SFTSV), we tested serum samples from rodents and shrews in China, collected in 2013. SFTSV antibodies and RNA were detected, suggesting that rodents and shrews might be hosts for SFTSV.     

RNA-Seq analysis and comparison of the enzymes involved in ionone synthesis of three cultivars of Osmanthus

Abstract

To comprehend the molecular mechanisms that control the differences in the composition of Osmanthus essential oils, the RNA-seq data and differentially expressed genes in different cultivar Osmanthus were studied. cDNA libraries of “jinqiugui,” “baijie,” and “rixianggui” were sequenced using Illumina HiSeq ^TM 2000. All of the enzymes involved in ionone synthesis were verified. DEGs were revealed and their enriched pathways were analyzed. A total of 20 DEGsencoding four enzymes that were potential candidates involved in ionone biosynthesis, as well as ispH, GPPS, ZDS, and CCD. It provided a way for Osmanthus oil monomer material to be synthesized in vitro.     

siRNAҩ���о���չ

??RNA interference technology(RNAi) has a great potential in the biomedical field, providing a very good idea for the research and development of new drugs because of its high efficiency, high specificity, and low toxicity. Rational design strategies, chemical modifications and nanocarriers offer significant opportunities to overcome the deficiencies of siRNA drugs such as rapid degradation, poor cell uptake and off-target effects. Here, the research progress of some siRNA drugs which have entered clinical trials was reviewed based on their indications, administration routes and nanocarriers, which provides references for researchers in development of siRNA drugs.     

佐剂诱导关节炎大鼠模型的建立及成纤维样滑膜细胞的分离

目的建立类风湿性关节炎(RA)动物模型；从炎性关节滑膜中分离成纤维样滑膜细胞(FLS)。方法应用热灭活结核杆菌H37Ra菌株与矿物油混合制备改良的佐剂,尾根部皮内注射Lewis大鼠诱导关节炎；剪取成功诱导关节炎大鼠的病变踝关节,从中剥离滑膜组织,充分剪碎后采用胶原酶消化法分离成纤维样滑膜细胞。结果成功诱导了大鼠关节炎,发病率为100%,发病时间有规律,组织学表现与RA相似；成功在体外培养了FLS并对其进行了鉴定,掌握了其生长形态和特征。结论成功制备RA动物模型并获得FLS,为今后RA发病机制探索和药物评价提供了良好的体内动物模型和体外细胞模型。