β-丙内酯在Vero细胞HFRS疫苗中的应用

目的 探讨β-丙内酯灭活双价Vero细胞肾综合征出血热纯化疫苗的最佳条件。方法 采用不同终浓度的β-丙内酯和不同的灭活时间对疫苗进行灭活，用细胞培养法进行病毒增殖试验，以确定灭活效果。应用高效液相色谱法分析β-丙内酯的最佳水解条件和3批双价Vero细胞出血热纯化灭活疫苗β-丙内酯残留虽。检测3批双价纯比灭活疫苗的免疫效果。结果 疫苗经终浓度为1：2000和1：4000的β-丙内酯作用24h，均可完全灭活病毒；疫苗中的β-丙内酯在37℃水浴下，随着水解时间的延长，其含量逐渐下降，水解2h后已无β-丙内酯检出；3批经β-丙内酯灭活的双价Vero细胞出血热纯化疫苗均未检出β-丙内酯残留，在家兔体内诱导产生针对汉滩型和汉城型病毒的中和抗体效价均达到1：20。结论 肾综合征出血热纯化疫苗经终浓度为1：4000β-丙内酪4℃灭活24h，然后再于37℃水浴中水解2h，既可达到完全灭活病毒且无β-丙内酯残留的目的；采用此方法灭活的双价Vero细胞肾综合征出血热纯化疫苗保持良好的免疫原性。     

Epinephrine-induced pulmonary oedema in rats is inhibited by corticotropin-releasing factor.

In various animal models of injury to skin, mucous membranes, muscle and brain, corticotropin-releasing factor (CRF) attenuated vascular leakage in the injured tissues. Here, the effects of CRF on a rat model of pulmonary oedema were examined. Male albino rats (220-290 g) received saline or CRF s.c., 30 min before pentobarbital anaesthesia, 60 mg/kg i.p., and 1 h before 1-epinephrine bitartrate (Epi), 30 micrograms/kg i.v. Within 30 min after Epi all (n = 27) saline-pretreated rats were dead from pulmonary oedema, but animals receiving human/rat CRF at doses of 7 to 57 micrograms/kg s.c. (n = 25) were all alive. Body wt, wet and dry wt of lungs were used to calculate an oedema index. This index increased from 3.6 +/- 0.1 to 9.6 +/- 0.3 after Epi but was inhibited by 87% after CRF 28 micrograms/kg s.c. The ED50 of CRF for reducing pulmonary oedema was 3.2 (1.3-7.4) micrograms/kg s.c. Mean arterial pressure increased from 119 +/- 4 to 167 +/- 2 mmHg after Epi 10 micrograms/kg i.v., but was not different (118 +/- 3 to 169 +/- 4 mmHg) after CRF pretreatment, 6 micrograms/kg s.c., a dose which reduced lung oedema. Pharmacokinetic estimates suggest that plasma levels of CRF sufficient to attenuate lung oedema in rats approximate those seen in pregnant women at delivery, raising the possibility that endogenous CRF may protect the maternal organism during parturition.     

99Tcm标记MIBI与MDP骨常规及延迟显像对比研究

目的探讨99Tcm-MIBI与99Tcm-MDP骨常规及其延迟显像对骨良恶性病变的诊断、鉴别诊断及疗效评估的临床应用价值及影响因素. 方法68例临床拟诊骨病变患者分别进行99Tcm-MDP和 99Tcm-MIBI骨显像;MIBI呈阳性者均行延迟显像;最终均行手术治疗及病理检查;其中12例于术前曾行化疗和/或放疗,治疗前后均行上述2项显像. 结果①99Tcm-MIBI显像76.3%(61/80病灶)恶性肿瘤病灶肉眼可见中、高度MIBI浓聚;而良性病灶仅有27.3%(6/22)的轻、中度浓聚;恶性病灶与对侧正常组织计数比值(L/C)为3.16±1.36明显高于良性病灶(1.35±0.56),P<0.01;延迟显像有利于良性病灶假阳性的鉴别.②99Tcm-MDP显像上述所有病灶均肉眼可见MDP浓聚,但恶性病灶L/C(3.66±1.27)与良性病灶L/C(2.97±1.03)比较差异无显著性(P>0.05).③化疗及放疗可以较明显抑制99Tcm-MIBI摄取(但放疗却可增加99Tcm-MDP摄取),因此99Tcm-MIBI摄取程度与99Tcm-MDP比较能较好反应治疗效果. 结论 99Tcm-MIBI骨常规及延迟显像对鉴别诊断良恶性骨病和评估疗效有较好的应用价值,若与99Tcm-MDP显像联合应用可提供更多有价值的信息.     

脉冲式电磁辐射对大鼠血脑屏障影响的量效关系

目的 研究脉冲式电磁辐射(EMR)对大鼠血脑屏障(BBB)影响的量效关系。方法 采用伊思蓝静脉注射，荧光显微镜下观察不同脉冲次数的EMR辐照对大鼠BBB开放的影响。结果 伊思蓝在EMR诱发的大鼠BBB开放局部呈现荧光斑；随着EMR脉冲次数(0～200次)的增加，荧光斑数量增加、面积增大；荧光斑在EMR组全脑的分布，以皮质、丘脑、下丘脑、小脑、尾壳核和延髓较多。结论 不同脉冲次数EMR诱发大鼠BBB开放程度不同，随脉冲次数增加，血管通透性增强，200次时达开放高峰，皮质区BBB开放最明显。     

Construction of an Rb gene expression plasmid.

We obtained an 844 bp Bg1II fragment from an Rb cDNA clone and inserted it into the expression vector pWR-13 to construct an Rb gene expression plasmid. When the Rb Bg1II fragment was fused in-frame into pWR-13, it was operated by a Lac Z promoter and produced a fusion protein which consisted of expressed Rb protein and a small peptide from Lac Z. The recombinants were transformed into E. coli with the CaCl2 method, screened by in situ hybridization, and restriction mapped. Total cellular protein of transformed clones was analyzed by SDS-PAGE and Commassie blue staining. The sense clones showed a unique band at 28,000. On Western blot, this band specifically reacted with 125I-labelled antibody against synthetic Rb peptide. This protein comprised more than 5% of total bacterial protein.     

内源性光动力疗法抑制人结肠癌裸鼠种植瘤的实验研究

目的　探讨基于 5 氨基乙酰丙酸 (ALA)的光动力疗法 (PDT)对结肠癌的生长抑制作用。方法　建立人结肠癌SW 480细胞裸鼠种植瘤模型 ,经尾静脉注入ALA液 (2 5 0mg/kg体重 ) ,光敏化 3h后半导体激光仪垂直照射肿瘤 3 0min(能量密度 9J/cm2 ) ,照射后连续观察肿瘤体积 ,瘤体HE染色病理分析。结果　ALA PDT在治疗后早期产生明显的抑制肿瘤增殖作用 ,瘤体组织坏死 ,腺腔样结构解离破坏 ,延命率 40 .2 % ,体积抑瘤率达 64 .1% ,治疗后期 ,肿瘤体积仍会缓慢增长 ,但增长速度明显小于对照组。结论　SALA PDT治疗可明显抑制裸鼠结肠种植瘤生长 ,延长荷瘤裸鼠生存期 ,但仍不能完全抑制肿瘤生长。     

髋臼骨折的手术治疗

目的：探讨髋臼骨折的手术治疗效果和影响疗效因素。方法：对31例髋臼骨折患进行切开复位由固定治疗。结果：平均随访18个月(6～36月)，根据Matta评分标准优16例，良8例，一般4例，差3例。1例术后发生深部感染，1例术后18个月发生股骨头坏死。结论：髋臼骨折，特别是有移位的髋臼骨折应及时手术治疗。手术医师的经验、骨折类型、手术时机和入路会影响髋臼骨折的疗效。     

大鼠癫痫持续状态黑质－腹侧被盖区多巴胺的组织化学研究

本实验用荧光组化技术及记录显微自动曝光时间方法研究了马桑内酯所致的Ｗｉｓｔａｒ大鼠癫痫持续状态黑质－腹侧被盖区多巴胺含量的变化。结果显示癫痫发作高峰组（１０只）和发作后组（８只）该区多巴胺含量明显低于对照组（１０只）和发作前组（１０只）。由于多巴胺是一种抑制性神经递质，它的减少可能表明抑制作用的下降对癫痫的发作有一定的调控作用。     

锤造全冠的适合性实验研究（三种缩颈方法的比较）

用实验方法比较三种常用的缩颈方法及评价锤造全冠的边缘适合性。结果表明各组边缘间隙均大于０．１８０ｍｍ，说明锤造全冠边缘适合性较差，临床检查难以确定其间隙大小。各组近胎面轴壁间隙较小，这与冠成形时此处受力较大，冠周径变小有关，提示临氏上应避免用力和反复锤击此处。各组胎面间隙均大于０．１３０ｍｍ，表明面升高，边缘浮出量较大，冠尚未完全就位。当硬铅代型与缩颈器之间未预留冠套间隙时，所得的锤造全冠适合性更差，面间隙最大（０．９４５ｍｍ）。     

Preoperative imaging of abnormal parathyroid glands in patients with hyperparathyroid disease using combination Tc-99m-pertechnetate and Tc-99m-sestamibi radionuclide scans.

OBJECTIVE: To evaluate the efficacy of combined Tc-99m-pertechnetate and Tc-99m-sestamibi radionuclide scanning for imaging abnormal parathyroid glands in hyperparathyroid disease in a prospective study. SUMMARY BACKGROUND DATA: Established methods to localize abnormal parathyroid glands lack accuracy for routine use. Tc-99m-sestamibi used in conjunction with iodine-123 has excellent potential for preoperative imaging in patients with hyperparathyroid disease. An alternative method for parathyroid imaging was studied using Tc-99m-pertechnetate and Tc-99m-sestamibi. METHODS: Thirty patients with hyperparathyroid disease had Tc-99m-pertechnetate and Tc-99m-sestamibi subtraction radionuclide scanning to visualize abnormal parathyroid glands before surgery. The patients had surgery and pathologic confirmation of all parathyroid glands. RESULTS: In 23 patients with primary hyperparathyroidism, 12 of 13 solitary adenomas were visualized. Six of nine patients with diffuse hyperplasia had bilateral uptake consistent with diffuse hyperplasia. Three of nine patients had negative scans. One patient previously operated on for diffuse hyperplasia had only one gland scanned. Seven patients with renal failure-associated hyperparathyroid disease were scanned: five had bilateral uptake of Tc-99m-sestamibi consistent with hyperplasia, and two who had been previously operated on had localization of remaining abnormal parathyroid glands. CONCLUSIONS: Tc-99m-pertechnetate combined with Tc-99m-sestamibi subtraction radionuclide scanning is less cumbersome to implement than iodine-123 combined with Tc-99m-sestamibi scanning. It has a high sensitivity for imaging solitary parathyroid adenomas or persistent solitary hyperplastic glands. However it does not have the resolution necessary to delineate all parathyroid glands in diffuse hyperplasia.