HPLC法测定蔓荆子黄素的含量

以蔓荆子黄素为指标,用反相 ＨＰＬＣ法测定了蔓荆子中的含量,实验采用 Ｃ_(１８)柱,以甲醇－０．４％磷酸溶液（６０： ４０）为流动相,检测波长 ２５８ｎｍ,用外标法测定,回收率为 ９８．１８％,ＲＳＤ１．４６％,线性范围 ０．１５～０．７５μｇ。本法快速简便,重现性好,为蔓荆子质量控制提供了可靠的依据。     

原代培养人脐动脉内皮细胞的超微结构

对不用贴附基质和生长因子情况下原代培养７２ｈ的人脐动脉内皮细胞（ＨＵＡＥＣ）进行了透射电镜观察。电镜下ＨＵＡＥＣ形态不规则,细胞器丰富。核周有散在的滑面内质网、粗面内质网及较多的线粒体,后者有些为板层状嵴,另一些为管泡状嵴。细胞膜下可见微管,胞质内有大量循同一方向排列的微丝。Ｗｅｉｂｅｌ Ｐａｌａｄｅ小体内含涡旋状排列的细管。胞质内含丰富的糖原颗粒。细胞核内常染色质较多,异染色质主要分布于核膜下。有时可见处于无丝分裂状态的细胞,核已分裂为二,但二者间仍有核膜相连。内皮细胞间以紧密连接或中间连接的方式相连。以上结果表明：人脐动脉内皮细胞在体外培养条件下代谢功能旺盛,且具有参与脂类代谢的细胞器,细胞以无丝分裂方式增殖。     

慢性肾功能衰竭中医证型标准化研究探讨

本文对２次全国性慢性肾衰中医辨证分型标准进行了分析和总结,并按现行慢性肾衰辨证分型方案对４４４例患者进行证候学研究,列出了慢性肾衰中出现率高且具有鉴别诊断价值的症状及体征,并发现慢性肾衰尿毒症患者其气血阴阳虚损的积分值明显高于其它各期,提出现行方案的不足之处及有待改进的探讨意见     

Clinical study on effect of Kang’aibao oral liquid in treating 103 patients of malignant tumor) in treating 103 patients of malignant tumor

Objective: To observe the clinical efficacy of Kang’aibao oral liquid (KABL), a Chinese herbal preparation in treating malignant tumor of middle or advanced stage.Methods: A comparative study was done by observing the effect of 103 patients treated with KABL, and another 90 patients treated with chemotherapy at the same time were taken as the control group.Results: The immunologic function, short-term effective rate (complete relieved rate and partial relieved rate), survival quality, 1-, 2- and 3-year survival rate, median survival time, and carcinoembryonic antigen (CEA) declining rate of the KABL group were significantly higher than those of the control group.Conclusion: KABL has the effects of inhibiting tumor growth, prolonging survival time and improving survival quality of tumor patients.     

The effect of Xin’an capsule on ventricular arrthythmia in animals) on ventricular arrthythmia in animals

Objective: To observe the effects of Xin’an capsule (XAC) on experimental ventricular arrhythmia (EVA) in animals and to explore the possible mechanism.Methods: Three types of animal arrhythmic model (ventricular arrythmia in guinea pigs induced by Ouabin, in rats caused by reperfusion after ligation of descending anterior branch of coronary artery and by CaCl₂) were used to study the effects of XAC on ventricular arrhythmia (VA).Results: After XAC treatment, the dosage of Ouabin needed for inducing ventricular premature beat (VPB), ventricular tachycardia (VT) and ventricular fibrillation (VF) increased in guinea pigs. The sustaining time of reperfusion induced VT and total occurrence of ventricular arrhythmias decreased. XAC also showed an effect in increasing CaCl₂ dosage needed to cause VA, and decreased the occurrence of VF and death in rats     

补肾健脾法治疗胎怯的临床研究

目的:研究补肾健脾法治疗胎怯的临床疗效。方法:以补肾健脾方药制成助长口服液为治疗组,以不用药为对照组,进行治疗胎怯的临床对照观察。结果:治疗组疗效(78%)显著优于对照组(52%),P<0.001;治疗组在改善体重、身长、头围、胸围、上臂围等方面显著优于对照组,P<0.05～0.01;治疗组治疗后血清T3升高,T4下降,与对照组相比,P<0.05;在降低患病率方面,治疗组(43%)优于对照组(72%),P<0.05;治疗组远期疗效优于对照组,P<0.05。结论:补肾健脾法治疗胎怯有显著的临床疗效。     

Effects of serum of Kawasaki disease on PDGF expression in monocytes and on endothelial cell apoptosis

ＴｈｅｃｏｒｏｎａｒｙａｒｔｅｒｙｃｏｍｐｌｉｃａｔｉｏｎｃｏｕｌｄｂｅｆｏｕｎｄｉｎａｌｍｏｓｔａｌｃａｓｅｓｏｆＫａｗａｓａｋｉｄｉｓｅａｓｅ［１］．Ｉｔｈａｓｒｅｃｅｎｔｌｙｂｅｅｎｓｈｏｗｎｔｈａｔｐｌａｔｅｌｅｔ－ｄｅｒｉｖｅｄｇｒｏｗｔｈ...     

Supravitale Morphologische Veränderungen der Skelettmuskulatur in frühen Postmortalen Zeitintervalen

Current Medical Science - Die morphologischen Veränderungen des mechanisch bzw. Elektrisch gereizten Skelettmuskels von 20 Leichen mit erhaltener supravitaler Reagibilitat in...     

动物气道反应性的测定

比较了两种测定动物气道反应性的方法： 整体容积描记法和气道内压法。发现前者能测定多个肺通气功能指标, 与临床可比性强, 结果精确, 其中０．１ ｓ用力呼气量 （ＦＥＶ０．１ ） 能较好地反映组胺刺激后气道收缩反应, 与组胺浓度呈显著负相关, 相关系数ｒ＝ －０．９６１; 后者测定方法简便, 重复性好, 能全程动态观察气道内压 （ＩＰ） 及其下降的最大速率（－ｄＩＰ／ｄｔｍ ａｘ） ,可较好反映组胺刺激后气道收缩反应,与组胺浓度呈显著正相关, ｒ分别为０．９６１ 及０．９７３,－ｄＩＰ／ｄｔｍ ａｘ 还可能是一种新的反映小气道功能的敏感指标。     

3种低温保存板层角膜移植材料的电镜观察

评价低温保存板层角膜移植材料的效果。方法将１５只人尸角膜分三组保存２年：４℃甘油脱水法,角膜液氮冷冻法,全眼球液氮冷冻法。并做透射电镜观察。结果第一组中,角膜胶原纤维排列紊乱,疏松,缺乏连续性,可见广纤维变性,纤维间可见极低电子密度灶。