全文获取类型
收费全文 | 10697篇 |
免费 | 1151篇 |
国内免费 | 99篇 |
专业分类
耳鼻咽喉 | 92篇 |
儿科学 | 334篇 |
妇产科学 | 272篇 |
基础医学 | 1335篇 |
口腔科学 | 204篇 |
临床医学 | 1105篇 |
内科学 | 2349篇 |
皮肤病学 | 115篇 |
神经病学 | 775篇 |
特种医学 | 783篇 |
外国民族医学 | 1篇 |
外科学 | 1178篇 |
综合类 | 335篇 |
一般理论 | 2篇 |
预防医学 | 1328篇 |
眼科学 | 314篇 |
药学 | 849篇 |
中国医学 | 9篇 |
肿瘤学 | 567篇 |
出版年
2021年 | 153篇 |
2019年 | 144篇 |
2018年 | 162篇 |
2017年 | 106篇 |
2016年 | 136篇 |
2015年 | 148篇 |
2014年 | 193篇 |
2013年 | 353篇 |
2012年 | 437篇 |
2011年 | 482篇 |
2010年 | 293篇 |
2009年 | 265篇 |
2008年 | 395篇 |
2007年 | 525篇 |
2006年 | 416篇 |
2005年 | 446篇 |
2004年 | 446篇 |
2003年 | 415篇 |
2002年 | 394篇 |
2001年 | 347篇 |
2000年 | 338篇 |
1999年 | 295篇 |
1998年 | 172篇 |
1997年 | 159篇 |
1996年 | 148篇 |
1995年 | 157篇 |
1994年 | 128篇 |
1993年 | 119篇 |
1992年 | 229篇 |
1991年 | 238篇 |
1990年 | 223篇 |
1989年 | 238篇 |
1988年 | 187篇 |
1987年 | 214篇 |
1986年 | 162篇 |
1985年 | 187篇 |
1984年 | 147篇 |
1983年 | 142篇 |
1982年 | 119篇 |
1981年 | 129篇 |
1980年 | 100篇 |
1979年 | 132篇 |
1978年 | 104篇 |
1977年 | 102篇 |
1976年 | 118篇 |
1975年 | 115篇 |
1974年 | 141篇 |
1973年 | 121篇 |
1972年 | 111篇 |
1971年 | 89篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
Mapping of a visceral leishmaniasis-specific immunodominant B-cell epitope of Leishmania donovani Hsp70.
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
G R Wallace A E Ball J MacFarlane S H el Safi M A Miles J M Kelly 《Infection and immunity》1992,60(7):2688-2693
We have shown that a member of the 70-kDa heat shock protein (Hsp70) family is a major target of the humoral immune response during Leishmania donovani infection. A recombinant fusion protein was recognized by sera from 92% (35 of 38) of patients with visceral leishmaniasis, including representatives from each of the major regions where it is endemic. Serological analysis of recombinant Hsp70, expressed by a series of deletion constructs, identified the carboxy-terminal region as the immunodominant site. This region, which is the most evolutionarily divergent part of the molecule, was recognized by all sera from patients with visceral leishmaniasis which exhibited an anti-Hsp70 response. Purified recombinant L. donovani Hsp70 was not recognized by sera from patients with cutaneous leishmaniasis, Chagas' disease, leprosy, malaria, or schistosomiasis. To determine the regions involved in antibody recognition, a series of overlapping peptides were synthesized on polyethylene pins by the Pepscan method, and a hexamer, EADDRA, was identified by the visceral leishmaniasis serum samples as an immunodominant B-cell epitope. 相似文献
112.
113.
Background: In recent years, non-syndromic idiopathic cardiomyopathies have increasingly been characterised as autosomal dominant conditions caused by single gene mutations. Loci have been identified for hypertrophic and dilated cardiomyopathy, and in some cases the same loci are associated with restrictive cardiomyopathy (RCM). In a kindred with RCM that we previously reported, we ruled out the known cardiomyopathy loci and other candidate genes by linkage analysis and mutation screening. Methods and Results: Here we report a genome-wide analysis in this family that has resulted in linkage to a region on chromosome 10. Conclusions: There are no genes in the interval that are known to cause idiopathic cardiomyopathy, and thus this linkage represents localisation of a new RCM locus. 相似文献
114.
Baker W Schneider BA Kulkarni A Sloan G Schaub R Sypek J Cannon JG 《Journal of leukocyte biology》2004,76(2):352-358
This investigation sought to determine if P-selectin-mediated mechanisms contributed to macrophage localization in damaged muscle, an essential process for muscle regeneration. Mice were injected intravenously (i.v.) with soluble P-selectin glycoprotein ligand-1 (sPSGL-1) at 5, 50, or 200 microg/mouse or with 100 microl vehicle alone, and then, lengthening contractions were induced in hindlimb plantar-flexor muscles. The contractions caused fiber damage in soleus muscles, with maximal invasion by CD11b+ mononuclear cells at 24 h post-injury and substantial accumulation of CD11b+ mononuclear cells in the extracellular matrix up to 7 days post-injury. sPSGL-1 treatment caused a dose-dependent decrease in the number of regenerating fibers (P=0.021), as determined by developmental myosin heavy chain (dMHC) expression. This expression was reduced 93% at 7 days post-injury by the highest dose of sPSGL-1, which had no significant influence on intrafiber or extracellular accumulation of cells expressing CD11b, a general marker for phagocytic cells. Additional mice were injected i.v. with 20 microg anti-P-selectin or isotype-control immunoglobulin G and were then subjected to lengthening contractions as before. At 7 days post-injury, soleus muscles from anti-P-selectin-treated mice contained 48% fewer mononuclear cells that bound ER-BMDM1 (P=0.019), a marker for mature macrophages and dendritic cells, and 84% fewer fibers expressing dMHC (P = 0.006), compared with muscles from isotype-injected, control mice. The number of CD11b+ cells was not significantly different between groups. The results are consistent with the concept that P-selectin is involved in the recruitment, maturation, and/or activation of cells that are critical for muscle fiber regeneration. 相似文献
115.
A mutation in the gene TNFRSF11B encoding osteoprotegerin causes an idiopathic hyperphosphatasia phenotype 总被引:5,自引:0,他引:5
116.
117.
118.
New Nocardia taxon among isolates of Nocardia brasiliensis associated with invasive disease. 总被引:5,自引:6,他引:5
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
R J Wallace Jr B A Brown Z Blacklock R Ulrich K Jost J M Brown M M McNeil G Onyi V A Steingrube J Gibson 《Journal of clinical microbiology》1995,33(6):1528-1533
Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 17-year period, were associated with extracutaneous and/or disseminated diseases. Of the 62 invasive isolates, 37 (60%) were susceptible to ciprofloxacin and/or were susceptible to clarithromycin and resistant to minocycline, compared with only 6 (3%) of 242 localized cutaneous isolates. The 43 isolates with this susceptibility pattern appeared to define a new taxon. They were similar to Nocardia asteroides complex isolates clinically in proportions from persons with pulmonary (70%), central nervous system (23%), and/or disseminated diseases (37%) in the setting of corticosteroids (74%) or AIDS (14%). This putative new taxon differed from N. brasiliensis in the hydrolysis of adenine (92 versus 4%), beta-lactamase patterns on isoelectric focusing, and the presence of two early mycolic acid-ester peaks by high-performance liquid chromatography. Restriction analysis of a 439-bp fragment of the 65-kDa heat shock protein gene revealed that N. brasiliensis and the new taxon had different restriction patterns with 8 of the 11 enzymes tested. Screening of invasive isolates of N. brasiliensis for susceptibility to ciprofloxacin will identify most isolates of the new taxon, which likely represents a new Nocardia species. 相似文献
119.
Neurofibromatosis 2 (NF2) is an inherited cancer syndrome resulting from
mutations in the NF2 tumor suppressor gene. Analysis of NF2 mutations has
revealed some general genotype-phenotype correlations. Severe disease has
been associated with mutations that produce a premature termination while
more mild disease has been associated with missense mutations. Here, we
provide experimental proof for these genotype-phenotype correlations by
demonstrating that nonsense mutations fail to produce stable merlin protein
while missense mutations result in the generation of merlin proteins
defective in negative growth regulation. This inability to suppress cell
growth may result from defects in the function of merlin at several levels,
including failure to form an intramolecular complex. Based on these
findings, we propose a model for merlin growth suppression that provides a
framework for analyzing NF2 patient mutations and merlin function.
相似文献
120.
Stein TP; Oram-Smith JC; Leskiw MJ; Wallace HW; Long LC; Leonard JM 《The American journal of physiology》1976,230(5):1321-1325