腹腔镜在原因不明性腹水的诊断中的应用

为了探讨腹腔镜在原因不明性腹水的病因诊断中的价值，对１１例经过Ｂ超、ＣＴ、腹水各种检查等仍然未明确诊断，并除外有心肝肺肾的疾病引起的腹水，在全麻下做腹腔镜检查、并取病变组织做病理学检查，１１例患者全部被确诊，结果表明：腹腔镜检查是诊断原因不明性腹水的有效方法，且并发症轻，可作为腹水病因诊断的一种常规检查     

北京石景山地区孕妇女婴幼儿血铅动态研究

在严格的质量保证措施下，北京石景山地区２７０名孕妇及其婴儿进行了血铅的追瞎调查。结果显示：孕３月血铅水平为４５．０μｇ／Ｌ，分娩前血铅水平为６４．８μｇ／Ｌ，孕期血铅呈升高趋势，其中后３月升高明显，分娩前血铅约为孕３月血铅的１．５倍；脐带血铅水平为５１．９μｇ／Ｌ，其中有１０．４％的胎儿脐血铅高于１００μｇ／Ｌ；     

Effects of Vagal and Sympathetic Nerves on Transmembrane Potential of Cardiac Ventricular Cells of Toad

The effects of vagal and sympathetic nerves on the transmembranepotentials of cardiac cells of toad were observed by means of microelectrodetechnique.The vagal nerve was stimulated there would be an increase in restingpotential and acceleration in repolarization of action potential(AP).However,ifatropine was used before stimulation the above-mentioned phenomena woulddisappear.When the sympathetic nerve was stimulated the AP amplitudeincreased,but resting potential(RP)remained the same.The increase of APresulted from the increases of overshoot.When the sympathetic nerve wasstimulated although the heart rate increased and the duration of AP wasshortened,the plateau phase of AP was prolonged.These results suggest that theeffects of vagal and sympathetic nerves on the transmembrane potential of cardiacventricular cells are coordinated and the normal characteritics of transmembranepotential are maintained by both the vagal and sympathetic nerves.     

MMP-2、TIMP-2在脑胶质瘤中的表达及其意义

目的 分析MMP-2、TIMP-2的表达与脑胶质瘤恶性程度和侵袭性之间的关系。方法 采用免疫组化和原位杂交的方法,检测42例人脑胶质瘤和8例正常脑组织中,MMP-2 mRNA、MMP-2和TIMP-2蛋白表达。结果 MMP-2蛋白表达水平(LI)与肿瘤的分级呈正相关(r=0.872,P<0.01);Ⅲ、Ⅳ级与正常脑组织、Ⅰ～Ⅱ级之间均有显著的差异(P<0.01)。MMP-2mRNA表达与肿瘤的分级同样呈正相关(P<0.01);Ⅲ、Ⅳ级与正常脑组织、Ⅰ～Ⅱ级之间均有显著的差异(P<0.01)。MMP-2蛋白表达增高时TIMP-2蛋白表达也增高;但随着肿瘤恶性程度的增加,TIMP-2表达强度增加的幅度较MMP-2明显减缓。结论 MMP-2的高表达与脑胶质瘤恶性程度呈正相关;TIMP-2与MMP-2间的平衡失调与脑胶质瘤的侵袭性和恶性程度密切相关。     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

假单胞菌胆固醇酯酶的生产Ⅰ.菌种筛选和发酵条件的研究

通过平板初筛和摇瓶复筛,从371株菌中筛得一株有工业生产价值的胆固醇酯酶产生菌,并对它进行了发酵条件的研究。用所获得的胆固醇酯酶配制的三酶试剂具有良好的稳定性,适用于临床检测。     

1020例不孕症的子宫及输卵管形态学分析

用复方泛影葡胺注射液对1,020例不孕症者作子宫输卵管造影。结果:子宫腔形态异常540例。双侧输卵管通畅但形态异常者268例。单侧输卵管通畅但形态异常者110例。结果表明:子宫输卵管形态异常与不孕有密切关系。     

2,7,12,18-四甲基-13,17-二(3-羟基丙基)卟啉的合成及其光敏化活性

由氯化高铁次卟啉经脱铁、酯化、还原合成了光敏剂２，７，１２，１８－四甲基－１３，１７－二（３－羟基丙基）卟啉，并测定其光敏活性     

元素释放器元素释放动态观察

应用我们实验室自制的元素释放器于１９９４－１９９６年进行了碘元素释放动态研究，取６个带有不同释放板的释放器和３个目前应用的缓释器为对照，每个均装入２克碘酸钾，置于水盐水中，应用溴氧化法每两周测定一次水碘含量。     

Biological characteristics of newly diagnosed poor prognosis acute myelogenous leukemia

A pilot study was conducted of the biological characteristics of the leukemia cells of newly diagnosed patients with poor prognosis acute myelogenous leukemia (AML). This study included measurements of the pretherapy proliferative rate of the leukemia cells in vivo, assessment of differentiation in vivo during remission induction therapy, and the level of expression of the fms, myc, and IL1β genes in pretherapy leukemia cells. Short cell cycle times were characteristic of the best prognostic category and were associated with a rapid reduction in marrow leukemia cells in cytosine arabinoside (araC)-sensitive patients. Expression of c-fms was associated with rapid reduction in marrow leukemia cells during araC therapy and with a successful treatment outcome. Expression of the IL1β gene was associated with short remissions. These studies suggest that when compared to newly diagnosed standard prognosis AML, the leukemia of poor prognosis patients is more likely to exhibit long cell cycle times, low levels of fms expression, and is less likely to be associated with myeloid differentiation during remission induction therapy. © 1993 Wiley-Liss, Inc.