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61.
Claus D. Eisenbach Edmund Stadler Volker Enkelmann 《Macromolecular chemistry and physics.》1995,196(3):833-856
The study of a series of molecularly uniform poly(N-alkylurethanes) with a systematically varied and tailored chain architecture has shown that the packing and superstructure can be controlled by the primary structure of the urethane chain and reversibly altered by the sample treatment, respectively. Depending on the conformation or the stereogeometry of the constitutive unit built in the middle of the otherwise symmetrical oligourethane, chain-extended and/or chain-folded crystallization of the urethane chain can occur. The packing order, i. e., adjacent recentry chain-folding or chain-extended crystallization, and the thermal properties of the oligourethanes are related to the chemical structure of the central constitutive unit. 相似文献
62.
Immunomagnetic enrichment and detection of isolated tumor cells in bone marrow of patients with epithelial malignancies 总被引:5,自引:0,他引:5
Weihrauch MR Skibowski E Draube A Geller A Tesch H Diehl V Bohlen H 《Clinical & experimental metastasis》2002,19(7):617-621
The detection of isolated tumor cells (ITC) in the bone marrow of patients with epithelial malignancies is an independant
prognostic factor for several entities as breast cancer, colorectal cancer or non-small lung cancer. However, with conventional
immunocytology using Ficoll density gradient and APAAP staining, only a small proportion of the bone marrow samples can be
scanned for cytokeratin-positive (CK+) cells. To improve detection rates, we evaluated the enrichment of ITC by magnetic activated
cell sorting (MACS) compared to regularly stained cytospins. Recovery experiments with a CK+ breast cancer cell line (SKBR3)
were performed to calculate the MACS enrichment rate. Bone marrow was obtained by aspiration from 20 patients with carcinomas
of epithelial origin and from 17 controls. ITC were enriched and stained with magnetically labeled CAM 5.2 antibodies directed
to cytokeratin 7 and 8. MACS of SKBR3 seeded in peripheral blood revealed average recovery rates of 62% and 48% and average
enrichment factors of 104-fold and 8139-fold of the CK+ cells after one and after two separations, respectively. After immunomagnetic
enrichment, CK+ cells were detected in 16 of 20 (80%) cancer patients, whereas only 7 (35%) patients showed CK+ cells without
magnetic enrichment (P=0.002). Ten of twelve (83%) patients with metastatic disease (stage M1) and six of eight (75%) patients without any overt metastases (M0) had CK+ cells in their bone marrow. None of the negative controls showed any CK+ cells. Enrichment with magnetically labeled
anti cytokeratin antibodies increases the detection rate of epithelial cells in bone marrow of cancer patients compared to
immunocytology.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
63.
Identification of a novel coronavirus in patients with severe acute respiratory syndrome 总被引:1,自引:0,他引:1
Drosten C Günther S Preiser W van der Werf S Brodt HR Becker S Rabenau H Panning M Kolesnikova L Fouchier RA Berger A Burguière AM Cinatl J Eickmann M Escriou N Grywna K Kramme S Manuguerra JC Müller S Rickerts V Stürmer M Vieth S Klenk HD Osterhaus AD Schmitz H Doerr HW 《The New England journal of medicine》2003,348(20):1967-1976
64.
The male genital system ofCephalobaena tetrapoda comprises the unpaired testis and seminal vesicle and the paired connecting tubes, ejaculatory bulbs, vasa deferentia, and dilators. These lead into the genital atrium, which can be everted to form the copulatory organ. An ultrastructural analysis of each of these compounds is presented, including spermiogenesis, which was traced in some important stages. The diverging nomenclature of the complicated male genital system was unified and partly changed: where the vas deferens and dilator merge, the former loses its epithelial lining; the cuticular tube is called the cirrus. Anteriorly, the dilator also gives rise to a cuticular, semilunar tube. The cirrus and semilunar tube run parallel and reach the genital atrium, which can be evaginated to form the copulatory organ. Apparently, the male genital system represents an original type from which the complicated porocephalic systems may be derived. 相似文献
65.
Masao Kaneko Dieter Whrle Derck Schlettwein Volker Schmidt 《Macromolecular chemistry and physics.》1988,189(10):2419-2423
Thin films (thickness 0,3 μm) of a mixture of phthalocyaninatozinc(II) ( 1 ) and poly(1-vinylcarbazole) ( 2 ) are prepared by coating from a solution on ITO glass. The blue coloured films are investigated in an electrochemical cell under irradiation with visible light. In the presence of dioxygen from air the films exhibit an intensive cathodic photocurrent. The action spectrum of the photocurrent corresponds to the absorption spectrum of the phthalocyanine. The results show a device which can be switched by air and visible light. 相似文献
66.
Hematopoietic progenitor cells and cellular microenvironment: behavioral and molecular changes upon interaction 总被引:2,自引:0,他引:2
Wagner W Saffrich R Wirkner U Eckstein V Blake J Ansorge A Schwager C Wein F Miesala K Ansorge W Ho AD 《Stem cells (Dayton, Ohio)》2005,23(8):1180-1191
Cell-cell contact between stem cells and cellular determinants of the microenvironment plays an essential role in controlling cell division. Using human hematopoietic progenitor cells (CD34+/CD38-) and a stroma cell line (AFT024) as a model, we have studied the initial behavioral and molecular sequel of this interaction. Time-lapse microscopy showed that CD34+/CD38- cells actively migrated toward and sought contact with stroma cells and 30% of them adhered firmly to AFT024 stroma through the uropod. CD44 and CD34 are colocalized at the site of contact. Gene expression profiles of CD34+/CD38- cells upon cultivation with or without stroma for 16, 20, 48, or 72 hours were analyzed using our human genome cDNA microarray. Chk1, egr1, and cxcl2 were among the first genes upregulated within 16 hours. Genes with the highest upregulation throughout the time course included tubulin genes, ezrin, c1qr1, fos, pcna, mcm6, ung, and dnmt1, genes that play an essential role in reorganization of the cytoskeleton system, stabilization of DNA, and methylation patterns. Our results demonstrate directed migration of CD34+/CD38- cells toward AFT024 and adhesion through the uropod and that upon interaction with supportive stroma, reorganization of the cytoskeleton system, regulation of cell division, and maintenance of genetic stability represent the most essential steps. 相似文献
67.
Hamid Hossain Volker Landgraf Reinhard Weiss Marion Mann Jaleh Hayatpour Trinad Chakraborty Peter Mayser 《Medical mycology》2007,45(1):41-49
The aim of the study was the characterization of Malassezia pachydermatis and its pigment-producing subgroup using biochemical tests and RAPD. It was of interest to determine whether particular RAPD patterns could be used to indicate pigment production, as well as a close genetic relatedness to Malassezia furfur. Therefore, 210 strains of M. pachydermatis were examined for morphology, catalase and ss-glucosidase activity, lipid and carbohydrate assimilation and the tryptophan-dependent synthesis of pigments. Of these, 114 strains were subjected to RAPD analyses. A multivariate logistic regression model was applied to classify M. pachydermatis isolates regarding their pigment production by using genetic and biological parameters. Biological and RAPD findings showed a high biological and genetic diversity within the species M. pachydermatis and within pigment producers. RAPD analysis revealed 28 genotypes within 114 strains tested. Pigment producing strains could not be assigned to a common RAPD profile, but a genetic relatedness of pigment-producing M. pachydermatis with M. furfur can be assumed. A particular RAPD pattern allowed statistically significant probability of pigment production (P<0.001) and might be used as a tool to rapidly detect pigment producing M. pachydermatis, e.g. in Malassezia-associated pityriasis versicolor. The reported method is useful for identification of pigment producing M. pachydermatis isolates and has advantages over established tests. 相似文献
68.
Kapp K Coustau C Wippersteg V Jourdane J Kunz W Grevelding CG 《Parasitology research》2003,91(6):482-485
Specific studies on schistosome gene functions require both access to the parasite stages, preferably the larvae, and to complete the life cycle. In the present study, we investigated whether short-term in vitro cultivation of sporocysts and surgical transplantation into snails could be combined to produce cercariae. Miracidia were maintained in vitro in the presence of Biomphalaria glabrata embryonic (Bge) cells or, alternatively, in Bge-cell-conditioned medium. The transformation of miracidia to mother sporocysts was observed in both cases. Two day-old sporocysts were transplanted into the cephalopedal sinus of recipient snails. Transplantation efficiencies varied between 16% and 43%, depending on the culture of the sporocysts in terms of the number of cercariae producing snails. Cercariae recovered from these snails were used to successfully infect hamsters, demonstrating that short term in vitro-generated sporocysts undergo normal cercariogenesis following transplantation. This combination of in vitro cultivation and transplantation may be useful for novel experimental approaches to investigate the genes involved in larval development or host-parasite molecular interactions. 相似文献
69.
Scheller C Sopper S Ehrhardt C Flory E Chen P Koutsilieri E Ludwig S ter Meulen V Jassoy C 《European journal of immunology》2002,32(9):2471-2480
CD95 is a major apoptosis receptor that induces caspase activation and programmed cell death in susceptible cells. CD95-induced apoptosis can be blocked by peptidic caspase inhibitors such as benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone or Ile-Glu-Thr-Asp-fluoromethyl ketone. Here we show that stimulation of CD95 in the presence of these inhibitors induces necrosis and expression of various proinflammatory cytokines in primary T lymphocytes, such as TNF-alpha, IFN-gamma and granulocyte/macrophage colony-stimulating factor. In the absence of caspase inhibition CD95 stimulation did not result in cytokine expression, indicating that this proinflammatory signaling pathway is suppressed by active caspases. Further analysis with A3.01 T cells revealed that the proinflammatory signaling activity of CD95 was mediated by MEK/ERK, p38 and NF-kappaB signaling pathways. These findings point to a pivotal role of caspases not only as mediators of apoptosis but also as enzymes that prevent proinflammatory signaling during CD95-induced apoptosis. Moreover, our findings may be useful for the development of novel pharmacological strategies. 相似文献
70.
In the pentastomidRaillietiella aegypti the structure of the genital apparatus and of oogonia, previtellogenic and vitellogenic oocytes was studied by light and electron microscopy. The ovarian wall consists of a single-layered epithelium covered by a basement membrane of varying thickness. The ovary is characterized by numerous, lateral, sac-like evaginations in which the oocytes lie.Centrioles indicate mitotic activities in oogonia. In previtellogenic oocytes the ooplasm is poor in organelles, containing only a few mitochondria and rough endoplasmic reticulum, but many free ribosomes. In vitellogenic oocytes there is a rapid multiplication of mitochondria and a proliferation of the rough endoplasmic reticulum. The cytoplasm also contains cortical vacuoles, yolk spheres, dictyosomes and pinocytotic vesicles. The mitochondria lie in one big cluster and combine to the Balbiani body (=yolk nucleus). Two types of yolk spheres differing in morphological details are formed in the oocytes ofR. aegypti. 相似文献