全文获取类型
收费全文 | 1514篇 |
免费 | 97篇 |
国内免费 | 22篇 |
专业分类
耳鼻咽喉 | 18篇 |
儿科学 | 69篇 |
妇产科学 | 14篇 |
基础医学 | 285篇 |
口腔科学 | 24篇 |
临床医学 | 135篇 |
内科学 | 278篇 |
皮肤病学 | 87篇 |
神经病学 | 159篇 |
特种医学 | 55篇 |
外科学 | 189篇 |
综合类 | 6篇 |
预防医学 | 84篇 |
眼科学 | 16篇 |
药学 | 65篇 |
肿瘤学 | 149篇 |
出版年
2023年 | 10篇 |
2022年 | 22篇 |
2021年 | 41篇 |
2020年 | 22篇 |
2019年 | 26篇 |
2018年 | 39篇 |
2017年 | 25篇 |
2016年 | 31篇 |
2015年 | 39篇 |
2014年 | 42篇 |
2013年 | 64篇 |
2012年 | 108篇 |
2011年 | 116篇 |
2010年 | 84篇 |
2009年 | 81篇 |
2008年 | 125篇 |
2007年 | 99篇 |
2006年 | 121篇 |
2005年 | 78篇 |
2004年 | 83篇 |
2003年 | 80篇 |
2002年 | 80篇 |
2001年 | 21篇 |
2000年 | 13篇 |
1999年 | 21篇 |
1998年 | 18篇 |
1997年 | 8篇 |
1996年 | 9篇 |
1995年 | 6篇 |
1994年 | 13篇 |
1993年 | 12篇 |
1992年 | 8篇 |
1991年 | 7篇 |
1990年 | 7篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 8篇 |
1979年 | 4篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 5篇 |
1966年 | 2篇 |
排序方式: 共有1633条查询结果,搜索用时 15 毫秒
11.
Yamamoto Raina; LI Xu; Winter Silke; Francke Uta; Kilimann Manfred W. 《Human molecular genetics》1995,4(2):265-268
Amphiphysin is a protein peripherally associated with synapticvesicles. It is expressed in many neurons, certain endocrinecell types, and spermatocytes. Autoantibodies against amphiphysinoccur in patients afflicted with a rare neurologic autoimmunedisease, paraneoplastic StiffMan syndrome. To providea basis for the understanding of antl-amphiphysin autolmmunity,we have cloned cDNAs and determined the primary structure ofhuman amphiphysin. Comparison with chicken amphiphysin definesdomains of low and high amino acid sequence conservation. Asa candidate for heritable disorders of the nervous system, endocrinetissues or male fertility, the human amphiphysin gene was mappedto chromosome 7, region p13p14. 相似文献
12.
Holger Hoehn Maria L. Rodriguez Thomas H. Norwood Clare L. Maxwell Uta Francke 《American journal of medical genetics. Part A》1978,2(3):253-266
The last decade has witnessed increasing application of human cytogenetic technology to prenatal chromosome analysis. However, unlike the rather uniform peripheral blood T-lymphocyte system which has provided most of our experience in human cytogenetics, long-term amniotic-fluid cell cultures display extreme cellular heterogeneity and disproportionate growth of certain cell types as a consequence of clonal amplification. When they enter cell culture, many of these cells are approching the terminal stages of their respective life spans and may have accumulated chromosomal aberrations. Concern about the possibility of true fetal mosaicism seems warranted chiefly in situations were multiple colonies display potentially viable aberrations. Clonal analysis, preferable of multiple clonal types, and attention to details of clonal morphology are likely to minimize diagnostic errors and undue apprehension resulting from mosaicism in amniotic-fluid cell cultures. 相似文献
13.
Vincent M. Riccardi Helen M. Hittner Uta Francke Jorge J. Yunis David Ledbetter Wayne Borges 《Cancer Genetics and Cytogenetics》1980,2(2):131-137
The role of del (11)(p13) as a cause of aniridia, with and without Wilms tumor, is strengthened by demonstration of this chromosome aberration in 3 patients: monozygous twin girls, both of whom have aniridia and mental retardation and one of whom has a Wilms tumor; and an unrelated boy with aniridia and ambiguous genitalia. The break points defining the interstitial deletion for the twins are 11p13 and 11p15.1, while for the boy they are 11p1302 and 11p14.1. These patients and their karyotypes substantiate the critical importance of chromosome band 11p13 (or its hemizygous representation) in the development of aniridia and an associated Wilms tumor diathesis, as had been suggested previously (Riccardi VM, Sujansky E, Smith AC, Francke U, (1978): Pediatrics 61, 604-610). 相似文献
14.
Kenneth M. Taylor Uta Francke Michael G. Brown Donna L. George Marilyn Kaufhold J. M. Opitz 《American journal of medical genetics. Part A》1977,1(1):3-19
We have studied 4 patients with inverted tandem duplications of parts of chromosomes, a hitherto rarely identified from of a structural rearrangement involving a single chromosome in man. In Patients 1 and 2, the duplication involved parts of the short arm of chromosome 8 (regions 8p 12→8p23 and 8p21→8p23, respectively). Both patients manifested certain characteristics of the mosaic trisomy 8 syndrome. Elevated levels of glutathione reductase (GSR) in their erythrocytes supported the interpretation of a partial duplication of chromosome 8 and indicated a regional localization for the GSR gene locus. In Patient 3, the distal half of the long arm of chromosome 4 was duplicated (region4q26→4q35). Clinical evidence supported this interpretation, as Patient 3 resembled phenotypically the 13 reported cases with duplication of the distal 4q. The cytogenetic findings in Patient 4 suggested a possibly inverted duplication of 22q. The clinical correlation was less convincing due to the lack of a well-defined phenotype for trisomy 22. These chromosome aberrations had occurred de novo in all 4 cases. Although they involved different chromosomal regions, they might well have arisen by the same mechanism. Possible modes of origin that are discussed in detail include unequal exchange between homologous chromosomes, between chromatids of 1 chromosome or between strands of 1 DNA duplex. 相似文献
15.
Hansen Julia Klusmann Uta Hanewinkel Reiner 《Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz》2022,65(7-8):776-783
Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz - Die COVID-19-Pandemie hat den beruflichen Alltag von Lehrpersonal erheblich verändert. Das Ziel dieser Arbeit ist es, die... 相似文献
16.
17.
Uta Landy Jema K Turk Kristin Simonson Katheryn Koenemann Jody Steinauer 《Contraception》2021,103(5):305-309
ObjectiveTo summarize the effects of routine, opt-out abortion and family planning residency training on obstetrics and gynecology (ob-gyn) residents’ clinical skills in uterine evacuation and intentions to provide abortion care after residency.MethodsData from ob-gyn residency programs supported during the first 20 years of the Kenneth J. Ryan Residency Training Program in Abortion and Family Planning were analyzed. Postrotation surveys assessed residents’ training experiences and acquisition of abortion care skills. Residency program director surveys assessed benefits of the training to residents and the academic department from the educators’ perspectives.ResultsA total of 2775 residents in 89 ob-gyn programs completed postrotation surveys for a response rate of 72%. During the rotation, residents – including those who only partially participated – gained exposure to and skills in first- and second-trimester abortion care. Sixty-one percent intended to provide abortion care in their postresidency practice. More than 90% of residency program directors (97.5% response rate) reported that training improved resident competence in abortion and contraception care and 81.3% reported that the training increased their own program's appeal to residency applicants.ConclusionOver 20 years, the Ryan Program has supported programs to integrate abortion training to give ob-gyn residents the skills and inspiration to provide comprehensive reproductive health care, including uterine evacuation and abortion care, in future practice. Residency program directors noted that this integrated training meets resident applicants’ expectations.ImplicationsRyan Program residents are trained to competence and are prepared, both clinically and in their professional attitudes, to care for women's reproductive health. 相似文献
18.
Kaneko K Inoue Y Nakano Y Kikuchi K Uta S 《Bulletin of the Hospital for Joint Diseases》1999,58(4):212-216
A spinal synovial cyst is a rare condition. We reported a case of lumbar spinal synovial cyst presenting neurological deficit. A 78-year-old woman was admitted to our hospital with the low back pain radiating to the left buttock. A myelography with computed tomography and magnetic resonance imaging revealed an extradural cystic lesion at the L5 and S1 level. At the time of surgery, a standard posterior approach was used to expose the posterior elements from L5 to S1. An en bloc laminectomy and total removal of the cyst was performed at the L5 to S1 level. The postoperative recovery was uneventful except for a slight pain in the left leg which persisted for some time. 相似文献
19.
20.
Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival. 总被引:17,自引:0,他引:17
Matthias Kappler Matthias Kotzsch Frank Bartel Susanne Füssel Christine Lautenschl?ger Uta Schmidt Peter Würl Matthias Bache Hannelore Schmidt Helge Taubert Axel Meye 《Clinical cancer research》2003,9(3):1098-1104
PURPOSE: Survivin is a member of the inhibitor-of-apoptosis gene family and is known to be overexpressed in a number of tumor types. The aim of this study was to evaluate the prognostic value of survivin protein expression in tumor tissue extracts in a group of well-characterized soft-tissue sarcoma (STS) patients. EXPERIMENTAL DESIGN: In this investigation, malignant tissue samples from 63 STS patients as well as from a panel of tumor cell lines were investigated, with nonmalignant tissues serving as controls. The survivin protein level was quantified by a novel ELISA and by Western blot analysis. Results obtained by both methods were compared with clinicopathological parameters regarding tumor grade and tumor entity, and they were then correlated to survival in a multivariate Cox regression model. RESULTS: High survivin levels were detected by ELISA and Western blot analysis in tumor tissue extracts and in lysates of tumor cell lines. None or only weak expression of survivin protein was found in nonmalignant cells and tissues. When comparing survivin values obtained by ELISA or Western blot, we found a significant correlation between both methods (P = 0.013, Pearson test). Our findings revealed that, in multivariate Cox regression analyses, survivin levels measured by ELISA and Western blot were significantly associated with tumor-related death in STS patients (P = 0.001, RR = 19.8, and P = 0.004, RR = 5.1, respectively). However, in a direct comparison of both survivin protein detection assays, we found a higher sensitivity and a stronger correlation to prognosis in survivin ELISA as compared with the Western blot assays. Furthermore, a higher tumor grade and more aggressive STS entity showed an elevated survivin protein expression level. CONCLUSION: Altogether, an elevated survivin content in tumor tissue extracts has a significant and independent negative predictive value on the survival-rate of STS patients. This finding corresponds well to data obtained for the mRNA level of survivin, as shown previously (M. Kappler et al., Int. J. Cancer, 95: 360-363, 2001). 相似文献