全文获取类型
收费全文 | 527篇 |
免费 | 19篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 7篇 |
妇产科学 | 5篇 |
基础医学 | 84篇 |
口腔科学 | 7篇 |
临床医学 | 44篇 |
内科学 | 85篇 |
皮肤病学 | 9篇 |
神经病学 | 20篇 |
特种医学 | 8篇 |
外科学 | 143篇 |
综合类 | 1篇 |
预防医学 | 28篇 |
药学 | 36篇 |
肿瘤学 | 70篇 |
出版年
2023年 | 4篇 |
2022年 | 8篇 |
2021年 | 13篇 |
2020年 | 6篇 |
2018年 | 9篇 |
2017年 | 9篇 |
2016年 | 3篇 |
2015年 | 12篇 |
2014年 | 6篇 |
2013年 | 19篇 |
2012年 | 15篇 |
2011年 | 25篇 |
2010年 | 15篇 |
2009年 | 6篇 |
2008年 | 22篇 |
2007年 | 24篇 |
2006年 | 18篇 |
2005年 | 21篇 |
2004年 | 18篇 |
2003年 | 21篇 |
2002年 | 18篇 |
2001年 | 15篇 |
2000年 | 27篇 |
1999年 | 23篇 |
1998年 | 6篇 |
1997年 | 10篇 |
1996年 | 7篇 |
1995年 | 2篇 |
1994年 | 7篇 |
1993年 | 3篇 |
1992年 | 23篇 |
1991年 | 9篇 |
1990年 | 20篇 |
1989年 | 14篇 |
1988年 | 14篇 |
1987年 | 11篇 |
1986年 | 13篇 |
1985年 | 6篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 6篇 |
1966年 | 2篇 |
排序方式: 共有548条查询结果,搜索用时 15 毫秒
61.
62.
Tsutomu Sakuma Katsuo Usuda Masashi Handa Gunji Okaniwa Tasuku Nakada Shigefumi Fujimura Michael A. Matthay 《Surgery today》1997,27(8):762-765
Alveolar fluid clearance was studied in the resected lung of a 58-year-old man who had undergone exploratory thoracotomy 9
days earlier. An isosmolar albumin solution was instilled into the distal air spaces, and the albumin and electrolyte concentrations
were measured over 4h. Alveolar sodium and fluid clearance had increased by nearly 200% from the control values in the resected
lungs of patients without prior thoracotomy (n=5), and histological examination showed that the number of alveolar type II epithelial cells was markedly elevated. These
results suggest that an increase in the number of alveolar type II cells may accelerate alveolar fluid clearance under certain
clinical conditions. 相似文献
63.
J Hayakawa G Yoshida Y Takakura Y Usuda 《Masui. The Japanese journal of anesthesiology》1992,41(12):1894-1899
We evaluated the influence of operative procedure on arterial ketone body ratio (AKBR), which indicates the function of the liver cells, in patients undergoing gastrectomy or mastectomy. AKBR during the stomach resection was significantly lower than that during the breast resection. A significant reduction in AKBR due to induced hypotension was observed in mastectomy group. SGOT and SGPT increased significantly in gastrectomy group, but unchanged in mastectomy group on the first and the seventh postoperative days. On the first post-operative day, SGOT and SGPT in gastrectomy group were significantly higher than those in mastectomy group. These findings suggest that influence of operative procedure on the liver during gastrectomy is more serious than that during mastectomy. 相似文献
64.
The standard methods of making electron microscopic radioautograms and quantitation of silver grains in our laboratory were
presented. Specialized techniques for insoluble or soluble compounds should be employed for demonstrating the synthesis of
various macromolecules and the incorporation of various labeled substances. Specimens for electron microscopic radioautography
processed under the same conditions can be employed for the quantitation of radioactive substances in subcellular compartments
using image analyzers. 相似文献
65.
Hiroki Takashima Yoshikatsu Koga Shino Manabe Kazunobu Ohnuki Ryo Tsumura Takahiro Anzai Nozomi Iwata Yang Wang Takuya Yokokita Yukiko Komori Daiki Mori Sachiko Usuda Hiromitsu Haba Hirofumi Fujii Yasuhiro Matsumura Masahiro Yasunaga 《Cancer science》2021,112(5):1975-1986
Tissue factor (TF), the trigger protein of the extrinsic blood coagulation cascade, is abundantly expressed in various cancers including gastric cancer. Anti-TF monoclonal antibodies (mAbs) capable of targeting cancers have been successfully applied to armed antibodies such as antibody-drug conjugates (ADCs) and molecular imaging probes. We prepared an anti-TF mAb, clone 1084, labeled with astatine-211 (211At), as a promising alpha emitter for cancer treatment. Alpha particles are characterized by high linear energy transfer and a range of 50-100 µm in tissue. Therefore, selective and efficient tumor accumulation of alpha emitters results in potent antitumor activities against cancer cells with minor effects on normal cells adjacent to the tumor. Although the 211At-conjugated clone 1084 (211At-anti-TF mAb) was disrupted by an 211At-induced radiochemical reaction, we demonstrated that astatinated anti-TF mAbs eluted in 0.6% or 1.2% sodium ascorbate (SA) solution were protected from antibody denaturation, which contributed to the maintenance of cellular binding activities and cytocidal effects of this immunoconjugate. Although body weight loss was observed in mice administered a 1.2% SA solution, the loss was transient and the radioprotectant seemed to be tolerable in vivo. In a high TF–expressing gastric cancer xenograft model, 211At-anti-TF mAb in 1.2% SA exerted a significantly greater antitumor effect than nonprotected 211At-anti-TF mAb. Moreover, the antitumor activities of the protected immunoconjugate in gastric cancer xenograft models were dependent on the level of TF in cancer cells. These findings suggest the clinical availability of the radioprotectant and applicability of clone 1084 to 211At-radioimmunotherapy. 相似文献
66.
67.
Post-stroke depression] 总被引:4,自引:0,他引:4
Yasuo Katayama Kazuhiro Usuda Yasuhiro Nishiyama Kenichiro Katsura 《Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics》2003,40(2):127-129
The frequency of post-stroke depression (PSD) was evaluated in an ischemic stroke cohort four weeks after onset, and the relationship between self-rating depression scale (SDS)/and infarct size, number and location of the ischemic brain lesions was also studied. The effects of a newly developed antidepressant SSRI (selective serotonin reuptake inhibitor), fluvoxamine maleate, on PSD and cerebral blood flow (CBF) was investigated in other ischemic stroke patients. The frequency of patients who had more than 40 on SDS score was 46% (18/39), and that of patients who had more than 50 was 13% (5/39). There were no differences in SDS score in infarct size, number and location of ischemic brain lesions, however there were significant differences in the lesion side. The score of the patients who had lesions in the left hemisphere was significantly higher than that of those who had them in the right. Administration of fluvoxamine maleate for four weeks improved the score on the Hamilton rating scale for depression (HAM-D) from 16.6 +/- 4.7 (n = 5) to 8.4 +/- 4.3 (n = 5), however it did not influence the mean cortical CBF. This study shows that the patients frequently had depression after ischemic stroke, and that left side lesion had a significant relationship with PSD. Therefore it is important that psychiatric examination of post-stroke patients is conducted. This study also shows that a newly developed antidepressant, fluvoxamine maleate, was effective for PSD. 相似文献
68.
69.
Azumi Hida Yuko Hasegawa Yuko Mekata Mika Usuda Yasunobu Masuda Hitoshi Kawano Yukari Kawano 《Nutrients》2012,4(10):1504-1517
The aim of this study was to evaluate the effects of egg white protein compared to carbohydrate intake prior to exercise on fat free mass (FFM), one repetition maximum (1RM) muscle strength and blood biochemistry in female athletes. Thirty healthy female collegiate athletes were recruited for this study and matched by sport type, body fat percentage and 1RM leg curl muscle strength. Participants were randomly divided into two groups: protein group (15.0 g egg white protein; 75 kcal) and carbohydrate group (17.5 g maltodextrin, 78 kcal). Supplements were administered daily at the same time in a double-blind manner prior to training during an 8-week period. Measurements were performed before and after the 8-week regimen. The mean dietary energy intake did not change throughout the study period. FFM and 1RM assessments (i.e., leg curl, leg extension, squat, and bench press) increased in both groups. Furthermore, serum urea and serum citrulline levels after the 8-week regimen increased significantly only in the protein group. Our findings indicated that compared to the carbohydrate supplement, the protein supplement was associated with some changes in protein metabolites but not with changes in body composition or muscle strength. 相似文献
70.
To elucidate differential roles of protein kinase C isozymes in pancreatic islet cells, the precise localization of the isozymes in rabbit and rat islet endocrine cells was investigated using monoclonal antibodies specific for three types of the enzyme. We detected strong immunoreactivity for the type III protein kinase C in B cells. Immunoreactivity for the type II enzyme was seen in A cells, and no apparent immunoreactivity for type I was observed in the islet cells. The expression of the type III protein kinase C in B cells was confirmed using rat insulinoma cells. The predominant expression of the type III enzyme in these cells was shown by immunoblotting. Moreover, on the basis of an enzyme-linked sandwich immunoassay, the levels of protein kinase C isozymes were determined in these cells. The significant amounts of the type III enzyme was detected, but the contents of the type I and II enzyme were under detectable level. These results suggest that the type III protein kinase C is involved in the regulation of insulin release in pancreatic B cells. 相似文献