Spleen Cells from Antithymocyte Serum Pretreated Mice Do Not Induce GVHD but Exert Increased Repopulating Activity in Irradiated Semiallogeneic Recipients

Graft-versus-host disease (GVHD) due to allogeneic bone marrow transplantation can be prevented by depleting the T cells from the marrow graft in vitro . However, the elimination of the donor T cells results in a higher frequency of graft failure, secondary infections and, in case of leukaemia, relapse. We found, that, in contrast to normal spleen cells, spleen cells from A or B10 donor mice pretreated with xenogeneic antithymocyte serum (ATS) in vivo did not induce GVHD in non-irradiated (B10 x A)F₁ hybrids. Spleen cells of ATS-pretreated A donors did not cause GVHD in allogeneic CBA mice made neonataily tolerant to the A donor strain either. Furthermore, spleen cells from ATS-treated donors did not cause GVHD in irradiated F₁ hybrid recipients, moreover, they decreased the lethal effect of irradiation. The in vivo ATS pretreatment improved the repopulating capacity of spleen cells in irradiated syngeneic recipients, too. The effect of the ATS treatment does not rely solely upon the elimination of T cells, since flow cytofluorometric analysis revealed only a partial depletion of both the CD4₊ and CD8₊ T cells of the ATS-pretreated animals. These observations may also have clinical relevance.     

SOLITARY BENIGN PAPILLOMA (PAPILLARY ADENOMA) OF THE CYSTIC DUCT: A RARE CAUSE OF BILIARY COLIC

SUMMARY Benign tumours of the extrahepatic bile ducts are rare, mostly occurring in the common bile duct and hepatic ducts. Solitary papillomas occur mainly in the gallbladder or at the ampulla of Vater.¹ Tumours of the cystic duct are extremely rare, and we are unaware of any reports of solitary papilloma of the cystic duct. Such a case in a 72-year-old woman is presented.     

吡酮酸类抗菌药物的研究——Ⅵ.7-氯-6-氟-1-乙基-1,4-二氢-4-氧吡啶(2,3-c)并哒嗪-3-羧酸及其衍生物的合成与抗菌活性

由2,6-二氯-5-氟-3-氰基吡啶出发,经氰基水解、酰氯化、与重氮乙酸乙酯缩合、重氮基还原、环化五步反应合成了一个新的杂环体系,即取代的吡啶(2,3-c)并哒嗪Ⅵ。由Ⅵ为原料经不同途径制得21个新衍生物,其中10个游离酸进行了体外抗菌活性筛选。对金葡-15、大肠-1515、绿脓-29的MIC(μg/ml),除化合物Xa和Xb对大肠-1515为50外,其余均大于100。     

竹叶椒化学成分的研究

自芸香科花椒属植物竹叶椒Zanthoxylum planispinum Sieb. et Zucc根的石油醚提取物中分离得到三种结晶。经物理常数测定,光谱(IR.NMR和MS)及X-射线单晶衍射分析,确定了化合物Ⅲ的绝对构型为(1R,2R,5R,6S)-2-(3′,4′-二甲氧基苯基)-6-(3″,4″-亚甲二氧苯基)双骈四氢呋喃,是一个新化合物,命名为竹叶椒脂素(L-planinin)。化合物Ⅰ为β-香木脂醇(β-amyrin),化合物Ⅱ为L-细辛脂素(L-asarinin),这三种化合物均系首次从本植物中分离得到。     

Evaluation of the biliary tract: The value of performing magnetic resonance cholangiopancreatography in conjunction with a 3‐D spoiled gradient‐echo gadolinium enhanced dynamic sequence

The 3‐D gradient‐echo (GRE) sequence allows thinner sections and better resolution of biliary obstruction. When the presence of biliary obstruction is identified using magnetic resonance cholangiopancreatography, the addition of the 3‐D GRE sequence may be helpful for diagnosing biliary obstruction. By showing the changes in the bile duct wall, within the duct lumen and around the bile duct, this technique can be helpful for distinguishing benign from malignant stricture as well as a stone from an enhancing intraluminal mass.