热变性法测定红色毛癣菌核中DNA鸟嘌呤加胞嘧啶含量的研究

DNA中鸟嘌呤加胞嘧啶克分子百分比含量(G Cmol％)值是一种间接反映两个物种核中DNA序列相似性的遗传指标之一.自从Lee和Belozersky把G Cmol％值用作细菌分类鉴定遗传指标以后,G Cmol％值在微生物分类鉴定方面已得到广泛应用。在真菌方面,国外于60年代已有报道,而国内     

Trisomy 18 phenotype in a patient with an isopseudodicentric 18 chromosome.

We report a female patient with a typical trisomy 18 phenotype who has a 46,XX, -18, +isopseudodic(18)(p11) karotype. The lack of features of the 18p- syndrome suggests that a significant amount of short arm material is present and that the Turner-like features associated with 18p- may be determined by monosomy for 18p11. The phenotype-genotype correlations in abnormalities affecting chromosome 18 are reviewed.     

脊柱胸腰段薄层断层解剖及临床意义

目的：明确脊柱胸腰段冠状、矢状和横断面解剖学特点，探索薄层断层切片在观测各髓节及脊神经根走行过程的临床应用价值。方法：采用40具成人脊柱胸腰段标本经改良火棉胶包埋法处理，制作16具冠状、8具矢状和16具横位的0．25mm厚连续切片，观察脊神经根走行于侧椎管和椎间管不同区段的解剖关系和椎间孔韧带分布特征，并直接测量相关结构参数。结果：观测了脊柱胸腰段A、B、C、D经4区横断层面、经椎间管内口矢状层面、经两侧椎弓根中心冠状层面的形态和相关参数。结论：三种方位断层切片可较好显示诸多结构位置关系，对脊髓或脊神经源性疾病诊疗和正确辨认手术视野所见具有重要价值。     

一次性注射器填充级HDPE专用料的研制

通过对母料载体树脂的筛选,无机填料的选择,添加性能优良的耐辐射助剂,制成了具有耐辐射性能、加工性能良好的母料,该母料的制备工艺简单。本文所述的填充级HDPE专用料是将HDPE与填充母料经过简单掺混制成的。经测试专用料满足国家医药标准YY 0114—93《医用输液、输血、注射器聚乙烯专用料》的要求。制品厂试验表明:该填充级的HDPE专用料加工性能良好,用该填充级HDPE专用料注射成型的注射器芯杆装配成一次性注射器满足国家专业标准ZBC 31009—87《一次性无菌注射器》的要求。该填充母料的加入可大大降低材料的成本,100份HDPE 7006A可添加67份填充母料,该填充级专用料的价格比HDPE树脂降低约1500元/吨,因此该填充级HDPE专用料的推广和使用具有良好的经济效益。     

细胞角蛋白19、galectin-3、HBME-1在甲状腺病变上的表达及鉴别诊断意义

目的 研究细胞角蛋白(CK)19、galectin(Gal)-3、HBME-1在甲状腺不同病变表达的特点及鉴别诊断中的应用价值。方法 应用免疫组织化学EnVision法检测了21例结节性甲状腺肿(结甲)、14例毒性甲状腺肿(甲亢)、15例甲状腺滤泡性腺瘤(腺瘤)、13例滤泡性癌、13例滤泡型乳头状癌及48例经典型乳头状癌中单克隆抗体CK19、Gal-3、HBME-1的表达。结果 甲状腺病变中3种标记表达均位于细胞质;CK19、Gal-3、HBME-1的表达在甲状腺良性病变(结甲、甲亢、腺瘤)大多为弱阳性或阴性,而滤泡性癌阳性明显增加、乳头状癌(滤泡型及经典型)大多为中、强阳性,3种标记在甲状腺不同病变的阳性表达率结甲为52．4％(11／21)、9．5％(2／21)、19．0％(4／21),甲亢为50．0％(7／14)、7．1％(1／14)、7．1％(1／14),腺瘤为60％(9／15)、13．3％(2／15)、13．3％(2／15),滤泡性癌为76．9％(10／13)、61．5％(8／13)、53．8％(7／13),滤泡型乳头状癌为：100％(13／13)、84．6％(11／13)、92．3％(12／13),经典型乳头状癌为100％(48／48)、93．8％(45／48)、95．8％(46／48);在甲状腺良性病变(结甲、甲亢、腺瘤)与恶性病变(滤泡性癌、乳头状癌)间3种标记差异均有显著性(P均=0．000);同时3种标记在滤泡样病变即腺瘤、滤泡性癌和滤泡型乳头状癌间亦有显著差异(CK19：P=0．038,Gal-3：P=0．001,HBME-1：P=0．000)。结甲有9例,甲亢有7例,腺瘤有6例3种标记均不表达,滤泡性癌仅有1例,而乳头状癌(滤泡型及经典型)没有病例3种标记均不表达,同一病例有2种以上阳性表达在结甲、甲亢、腺瘤、滤泡性癌、滤泡型乳头状癌和经典型乳头状癌中分别为14．2％(3／21)、21．4％(3／14)、20．0％(3／15)、69．2％(9／13)、92．3％(12／13)、100．0%(48／48),在甲状腺良性病变与恶性病变间以及滤泡样病变间差异亦有显著性(P=0．000)。结论 CK19、Gal-3、HBME-1的检测尤其是联合检测对甲状腺病变的诊断、鉴别诊断具有较高的实用价值。     

Translocation of Enterococcus faecalis strains across a monolayer of polarized human enterocyte-like T84 cells

We used a two-chamber system to study transcytosis of Enterococcus faecalis across monolayers of human colon carcinoma-derived T84 cells, which show structural resemblance to the native intestine. Among 16 E. faecalis isolates from different sources, the well-characterized strain OG1RF and 8 other isolates (2 endocarditis isolates, 1 urine isolate, and all 5 fecal isolates) showed translocation in this assay, while 6 clinical isolates (3 endocarditis and 3 urine isolates), the recipient strain JH2-2, and the control, Escherichia coli DH5alpha, had no detectable translocation. Of two OG1RF mutants involving the previously studied epa (enterococcal polysaccharide antigen) gene cluster, known to be needed for virulence and resistance to killing by polymorphonuclear leukocytes, one epa mutant (TX5179) was unable to translocate, while TX5180, with an epa disruption farther downstream, showed a moderate decrease in translocation relative to that of the wild-type strain OG1RF (P < 0.01), indicating that the epa gene cluster is important for translocation across a T84 monolayer. This observation was confirmed by complementation of the epa mutant (TX5179) with epa genes and restoration of its translocation ability. In conclusion, we have demonstrated translocation of at least some strains of E. faecalis across T84 monolayers, although strains differ considerably in this ability, and we have demonstrated that epa mutations can cause marked changes in successful translocation. These results suggest that this model may be a useful in vitro system for studying the process of translocation from the intestinal tract.