全文获取类型
收费全文 | 6412篇 |
免费 | 644篇 |
国内免费 | 345篇 |
专业分类
耳鼻咽喉 | 37篇 |
儿科学 | 74篇 |
妇产科学 | 164篇 |
基础医学 | 842篇 |
口腔科学 | 91篇 |
临床医学 | 778篇 |
内科学 | 1094篇 |
皮肤病学 | 60篇 |
神经病学 | 412篇 |
特种医学 | 280篇 |
外国民族医学 | 3篇 |
外科学 | 651篇 |
综合类 | 850篇 |
现状与发展 | 1篇 |
一般理论 | 2篇 |
预防医学 | 326篇 |
眼科学 | 140篇 |
药学 | 732篇 |
5篇 | |
中国医学 | 293篇 |
肿瘤学 | 566篇 |
出版年
2024年 | 28篇 |
2023年 | 113篇 |
2022年 | 275篇 |
2021年 | 358篇 |
2020年 | 285篇 |
2019年 | 263篇 |
2018年 | 267篇 |
2017年 | 211篇 |
2016年 | 172篇 |
2015年 | 331篇 |
2014年 | 378篇 |
2013年 | 326篇 |
2012年 | 531篇 |
2011年 | 539篇 |
2010年 | 300篇 |
2009年 | 218篇 |
2008年 | 290篇 |
2007年 | 292篇 |
2006年 | 318篇 |
2005年 | 366篇 |
2004年 | 182篇 |
2003年 | 178篇 |
2002年 | 140篇 |
2001年 | 108篇 |
2000年 | 120篇 |
1999年 | 118篇 |
1998年 | 58篇 |
1997年 | 54篇 |
1996年 | 52篇 |
1995年 | 47篇 |
1994年 | 57篇 |
1993年 | 23篇 |
1992年 | 52篇 |
1991年 | 53篇 |
1990年 | 37篇 |
1989年 | 41篇 |
1988年 | 28篇 |
1987年 | 30篇 |
1986年 | 15篇 |
1985年 | 18篇 |
1984年 | 18篇 |
1983年 | 5篇 |
1982年 | 10篇 |
1981年 | 7篇 |
1979年 | 10篇 |
1978年 | 16篇 |
1973年 | 10篇 |
1972年 | 6篇 |
1967年 | 7篇 |
1966年 | 5篇 |
排序方式: 共有7401条查询结果,搜索用时 15 毫秒
21.
Trisomy 18 phenotype in a patient with an isopseudodicentric 18 chromosome. 总被引:1,自引:1,他引:1 下载免费PDF全文
We report a female patient with a typical trisomy 18 phenotype who has a 46,XX, -18, +isopseudodic(18)(p11) karotype. The lack of features of the 18p- syndrome suggests that a significant amount of short arm material is present and that the Turner-like features associated with 18p- may be determined by monosomy for 18p11. The phenotype-genotype correlations in abnormalities affecting chromosome 18 are reviewed. 相似文献
22.
23.
通过对母料载体树脂的筛选,无机填料的选择,添加性能优良的耐辐射助剂,制成了具有耐辐射性能、加工性能良好的母料,该母料的制备工艺简单。本文所述的填充级HDPE专用料是将HDPE与填充母料经过简单掺混制成的。经测试专用料满足国家医药标准YY 0114—93《医用输液、输血、注射器聚乙烯专用料》的要求。制品厂试验表明:该填充级的HDPE专用料加工性能良好,用该填充级HDPE专用料注射成型的注射器芯杆装配成一次性注射器满足国家专业标准ZBC 31009—87《一次性无菌注射器》的要求。该填充母料的加入可大大降低材料的成本,100份HDPE 7006A可添加67份填充母料,该填充级专用料的价格比HDPE树脂降低约1500元/吨,因此该填充级HDPE专用料的推广和使用具有良好的经济效益。 相似文献
24.
细胞角蛋白19、galectin-3、HBME-1在甲状腺病变上的表达及鉴别诊断意义 总被引:12,自引:2,他引:12
目的 研究细胞角蛋白(CK)19、galectin(Gal)-3、HBME-1在甲状腺不同病变表达的特点及鉴别诊断中的应用价值。方法 应用免疫组织化学EnVision法检测了21例结节性甲状腺肿(结甲)、14例毒性甲状腺肿(甲亢)、15例甲状腺滤泡性腺瘤(腺瘤)、13例滤泡性癌、13例滤泡型乳头状癌及48例经典型乳头状癌中单克隆抗体CK19、Gal-3、HBME-1的表达。结果 甲状腺病变中3种标记表达均位于细胞质;CK19、Gal-3、HBME-1的表达在甲状腺良性病变(结甲、甲亢、腺瘤)大多为弱阳性或阴性,而滤泡性癌阳性明显增加、乳头状癌(滤泡型及经典型)大多为中、强阳性,3种标记在甲状腺不同病变的阳性表达率结甲为52.4%(11/21)、9.5%(2/21)、19.0%(4/21),甲亢为50.0%(7/14)、7.1%(1/14)、7.1%(1/14),腺瘤为60%(9/15)、13.3%(2/15)、13.3%(2/15),滤泡性癌为76.9%(10/13)、61.5%(8/13)、53.8%(7/13),滤泡型乳头状癌为:100%(13/13)、84.6%(11/13)、92.3%(12/13),经典型乳头状癌为100%(48/48)、93.8%(45/48)、95.8%(46/48);在甲状腺良性病变(结甲、甲亢、腺瘤)与恶性病变(滤泡性癌、乳头状癌)间3种标记差异均有显著性(P均=0.000);同时3种标记在滤泡样病变即腺瘤、滤泡性癌和滤泡型乳头状癌间亦有显著差异(CK19:P=0.038,Gal-3:P=0.001,HBME-1:P=0.000)。结甲有9例,甲亢有7例,腺瘤有6例3种标记均不表达,滤泡性癌仅有1例,而乳头状癌(滤泡型及经典型)没有病例3种标记均不表达,同一病例有2种以上阳性表达在结甲、甲亢、腺瘤、滤泡性癌、滤泡型乳头状癌和经典型乳头状癌中分别为14.2%(3/21)、21.4%(3/14)、20.0%(3/15)、69.2%(9/13)、92.3%(12/13)、100.0%(48/48),在甲状腺良性病变与恶性病变间以及滤泡样病变间差异亦有显著性(P=0.000)。结论 CK19、Gal-3、HBME-1的检测尤其是联合检测对甲状腺病变的诊断、鉴别诊断具有较高的实用价值。 相似文献
25.
Translocation of Enterococcus faecalis strains across a monolayer of polarized human enterocyte-like T84 cells 下载免费PDF全文
We used a two-chamber system to study transcytosis of Enterococcus faecalis across monolayers of human colon carcinoma-derived T84 cells, which show structural resemblance to the native intestine. Among 16 E. faecalis isolates from different sources, the well-characterized strain OG1RF and 8 other isolates (2 endocarditis isolates, 1 urine isolate, and all 5 fecal isolates) showed translocation in this assay, while 6 clinical isolates (3 endocarditis and 3 urine isolates), the recipient strain JH2-2, and the control, Escherichia coli DH5alpha, had no detectable translocation. Of two OG1RF mutants involving the previously studied epa (enterococcal polysaccharide antigen) gene cluster, known to be needed for virulence and resistance to killing by polymorphonuclear leukocytes, one epa mutant (TX5179) was unable to translocate, while TX5180, with an epa disruption farther downstream, showed a moderate decrease in translocation relative to that of the wild-type strain OG1RF (P < 0.01), indicating that the epa gene cluster is important for translocation across a T84 monolayer. This observation was confirmed by complementation of the epa mutant (TX5179) with epa genes and restoration of its translocation ability. In conclusion, we have demonstrated translocation of at least some strains of E. faecalis across T84 monolayers, although strains differ considerably in this ability, and we have demonstrated that epa mutations can cause marked changes in successful translocation. These results suggest that this model may be a useful in vitro system for studying the process of translocation from the intestinal tract. 相似文献
26.
Efficient generation of respiratory syncytial virus (RSV)-neutralizing human MoAbs via human peripheral blood lymphocyte (hu-PBL)-SCID mice and scFv phage display libraries 总被引:2,自引:0,他引:2 下载免费PDF全文
Nguyen H Hay J Mazzulli T Gallinger S Sandhu J Teng Y Hozumi N 《Clinical and experimental immunology》2000,122(1):85-93
RSV is one of the major causes of pneumonia and bronchiolitis in infants and young children and is associated with high mortality. RSV neutralizing human antibody (hu-Ab) is known to mediate resistance to viral infection as well as to be an effective treatment for severe lower respiratory tract RSV infection. We have previously demonstrated that human primary and secondary immune responses can be established in severe combined immunodeficient mice engrafted with human peripheral blood lymphocytes (hu-PBL-SCID). By combining this animal model with the single-chain Fv antibody (scFv) phage display library technique, we were able to investigate further its clinical potential by generating a panel of human scFvs that exhibit both high F glycoprotein (RSV-F) binding affinities ( approximately 108 M(-1)) and strong neutralizing activities against RSV infection in vitro. Sequencing analysis of the randomly isolated anti-RSV-F scFv clones revealed that they were derived from different VH families with mutations in the complementarity-determining region 1 (CDR1). The results suggest that: (i) RSV-F-specific human immune responses and affinity maturation can be induced in hu-PBL-SCID mice; and (ii) this approach can be applied to generate large numbers of human scFvs with therapeutic potential. Despite the fact that hu-PBL-SCID mouse and human scFv phage display library have individually been established, our approach contributes a simple and significant step toward the generalization of antigen-specific human monoclonal antibody (hu-MoAb) production and their clinical applications. 相似文献
27.
研究新鲜人羊膜的变应原性及其致敏后发生I型超敏反应的可能性。建立豚鼠全身主动过敏实验模型。分新鲜羊膜组、新鲜蛋清组(阳性对照)和PBS液组(阴性对照),每组10只豚鼠。观察豚鼠在致敏期和激发后的反应,采用化学荧光法检测外周血组胺含量,血液流变分析系统检测4项血液流变学指标(全血高切变率黏度、全血低切变率黏度、血浆黏度、红细胞聚集指数)。致敏期间各组豚鼠的体重变化无明显差别(P>0.05);激发后羊膜组豚鼠与阴性组表现一致,无异常反应;羊膜组外周血组胺含量及4项血液流变学指标均与阴性对照无明显差别(P>0.05),与阳性对照有显著性差异(P<0.01)。经规范化无菌处理后的新鲜羊膜,一般不具有变应原性,不会引起I型超敏反应。 相似文献
28.
刺激颈部交感神经节对椎动脉血流影响的实验研究 总被引:13,自引:0,他引:13
目的 :探讨颈部交感神经因素在造成椎 -基底动脉系统缺血过程中的作用。方法 :健康家兔3 0只 ,手术暴露颈部交感神经节及基底动脉 ,不同方式刺激颈部交感神经节 ,记录、分析基底动脉血流的变化。结果 :刺激前测得基底动脉基线血流为 (5 48± 2 7)PU :分别刺激颈上、中、下交感神经节后测得基底动脉血流为 (5 2 9± 16)PU、(4 74± 16)PU和 (3 70± 3 6)PU。与基线血流相比分别下降了 3 .4%、13 .5 %和 3 2 .5 %。颈部交感神经节阻滞并不能使正常状态下的基底动脉血流增加 ,但可以阻断交感神经缩血管作用。结论 :颈部交感神经受到刺激 ,可造成椎 -基底动脉系统供血不足。 相似文献
29.
Jihai Xu Jing Wu Xiaofeng Teng Libing Cai Huizong Yuan Xiaokun Chen Mu Hu Xin Wang Ning Jiang Hong Chen 《American journal of medical genetics. Part A》2020,182(9):2117-2123
Polydactyly and syndactyly are digital abnormalities in limb‐associated birth defects usually caused by genetic disorders. In this study, a five‐generation Chinese pedigree was found with triphalangeal thumb polysyndactyly syndrome (TPTPS), showing an autosomal dominant pattern of inheritance. We utilized linkage analysis and whole genome sequencing (WGS) for the genetic diagnosis of this pedigree. Linkage analysis was performed using a genome‐wide single nucleotide polymorphism (SNP) chip and three genomic regions were identified in chromosomes 2, 6, and 7 with significant linkage signals. WGS discovered a copy number variation (CNV) mutation caused by a large duplication region at the tail of chromosome 7 located in exons 1–5 of the LMBR1 gene, including the zone of polarizing activity regulatory sequence (ZRS), with a length of approximately 180 kb. A real‐time polymerase chain reaction (PCR) assay confirmed the duplication. The findings of our study supported the notion that large duplications including the ZRS caused TPTPS. Our study showed that linkage analysis in combination with WGS could successfully identify the disease locus and causative mutation in TPTPS, which could help elucidate the molecular mechanisms and genotype–phenotype correlations in polydactyly. 相似文献
30.
Determination of Enterococcus faecalis groESL full-length sequence and application for species identification 下载免费PDF全文
Amplification of the partial Cpn60 (or GroEL) gene segment has been used for identification of many bacteria, including Enterococcus species. To obtain more sequence data from groESL genes of Enterococcus faecalis, the full-length sequence of the E. faecalis groESL genes containing groES (285 bp), spacer (57 bp), and groEL (1,626 bp) was determined. A database search of GenBank revealed that the deduced E. faecalis GroES and GroEL proteins show significant homology to the GroES and GroEL proteins of other bacteria. The GroEL (groEL) of E. faecalis had the highest identity with Streptococcus pneumoniae (81.8% amino acid sequence identity and 73.0% nucleotide sequence identity), followed by Lactococcus zeae, while GroES (groES) had 60.2% (64.6%) identity with Lactobacillus zeae and 58.5% (66.2%) identity with Lactococcus lactis, followed by 57.0% (65.5%) identity with Bacillus subtilis. Based on the groES sequence, an E. faecalis-specific PCR assay was developed, and this PCR assay was positive for all the E. faecalis strains tested. Dot blot hybridization using either groES or groEL as the probe distinguished E. faecalis clearly from other species, indicating that both genes can be used as suitable targets for E. faecalis identification. Moreover, broad-range PCR-restriction fragment length polymorphism of groESL was designed to differentiate eight commonly encountered Enterococcus species. The Enterococcus species of reference strains could be easily differentiated on the basis of restriction patterns produced by HaeIII and RsaI. The DNA-based assays developed in this study provide an alternative to currently used methods of identification for clinically important enterococcal species. 相似文献