全文获取类型
收费全文 | 927篇 |
免费 | 56篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 33篇 |
妇产科学 | 12篇 |
基础医学 | 91篇 |
口腔科学 | 22篇 |
临床医学 | 93篇 |
内科学 | 174篇 |
皮肤病学 | 7篇 |
神经病学 | 55篇 |
特种医学 | 97篇 |
外科学 | 145篇 |
综合类 | 16篇 |
预防医学 | 82篇 |
眼科学 | 34篇 |
药学 | 74篇 |
肿瘤学 | 45篇 |
出版年
2022年 | 6篇 |
2021年 | 10篇 |
2020年 | 10篇 |
2019年 | 13篇 |
2018年 | 20篇 |
2017年 | 10篇 |
2016年 | 16篇 |
2015年 | 16篇 |
2014年 | 22篇 |
2013年 | 33篇 |
2012年 | 44篇 |
2011年 | 34篇 |
2010年 | 33篇 |
2009年 | 37篇 |
2008年 | 21篇 |
2007年 | 28篇 |
2006年 | 19篇 |
2005年 | 25篇 |
2004年 | 20篇 |
2003年 | 15篇 |
2002年 | 20篇 |
2001年 | 14篇 |
2000年 | 9篇 |
1999年 | 20篇 |
1998年 | 27篇 |
1997年 | 29篇 |
1996年 | 37篇 |
1995年 | 26篇 |
1994年 | 28篇 |
1993年 | 22篇 |
1992年 | 16篇 |
1991年 | 18篇 |
1990年 | 20篇 |
1989年 | 30篇 |
1988年 | 15篇 |
1987年 | 26篇 |
1986年 | 30篇 |
1985年 | 13篇 |
1984年 | 12篇 |
1983年 | 8篇 |
1982年 | 8篇 |
1981年 | 14篇 |
1980年 | 9篇 |
1979年 | 11篇 |
1978年 | 8篇 |
1977年 | 6篇 |
1976年 | 12篇 |
1975年 | 8篇 |
1970年 | 9篇 |
1966年 | 9篇 |
排序方式: 共有989条查询结果,搜索用时 15 毫秒
101.
We report a new bleeding disease--storage pool deficiency (SPD) of platelets--in pigs from the Mayo swine colony of homozygous von Willebrand's disease (vWD) and of heterozygous carriers of vWD. Levels of factor VIII, von Willebrand factor antigen (vWF:Ag), and ristocetin cofactor (RCof) were similar in the vWD carriers and SPD pigs. The latter pigs, however, had bleeding times of 15 minutes or more and were severe bleeders, in contrast to clinically normal vWD carriers. Platelet aggregation in response to collagen was reduced in most SPD pigs. Total platelet content of ADP, ATP, and serotonin was less than that of normal pigs. While the initial uptake of 14C-labeled serotonin into platelets was similar in SPD and normal pigs, retention of serotonin was reduced in platelets of SPD pigs. Transmission electron microscopy showed a large decrease of dense bodies in the platelets of SPD pigs. These findings support a diagnosis of SPD. Genetic analyses suggest an autosomal recessive mode of inheritance. A breeding program is under way to produce pigs affected only at the SPD gene, thus allowing further characterization of SPD and SPD-carrier pigs. 相似文献
102.
Molecular basis of the heterogeneity of expression of glycosyl phosphatidylinositol anchored proteins in paroxysmal nocturnal hemoglobinuria 总被引:2,自引:3,他引:2
Endo M; Ware RE; Vreeke TM; Singh SP; Howard TA; Tomita A; Holguin MH; Parker CJ 《Blood》1996,87(6):2546-2557
The purpose of these studies was to determine the molecular basis of the phenotypic mosaicism that is a defining feature of paroxysmal nocturnal hemoglobinuria (PNH). Analysis of T cell clones from a female patient revealed four distinct phenotypes based on surface expression of glycosyl phosphatidylinositol-anchored proteins (GPI-AP). When PIG-A (the gene that is mutant in PNH) from these clones was analyzed, four discrete somatic mutations were identified. Analysis of X chromosomal inactivation among the abnormal T cell clones was consistent with polyclonality. Together, these studies demonstrate that the phenotypic mosaicism that is characteristic of PNH is a consequence of genotypic mosaicism and that, at least in this case, PNH is a polyclonal rather than a monoclonal disease. That four distinct somatic mutations were present in a single patient suggests that in conditions that predispose to PNH PIG-A may be hypermutable. 相似文献
103.
D R Lambert P P Llaneza R D Gaglani R D Lach W L Beaver 《Journal of clinical gastroenterology》1987,9(3):345-349
We report an unusual case of an esophageal-atrial fistula in a patient with CREST (calcinosis, Raynaud's phenomenon, esophagitis, sclerodactyly, telangiectasia) variant of scleroderma. An ulcer in Barrett's esophagus perforated into the left atrium and led to systemic embolization and cerebral abscess. A review of similar reports of esophageal-atrial fistula reveals a symptom complex that includes chronic esophageal pathology, gastrointestinal bleeding, and neurological signs. An antemortem diagnosis has never been made. 相似文献
104.
Frequency and significance of serum alpha-fetoprotein elevation in severe hepatitis B surface antigen-negative chronic active hepatitis 总被引:1,自引:0,他引:1
To determine the frequency and significance of alpha-fetoprotein elevation in severe hepatitis B surface antigen-negative chronic active hepatitis, 558 serum samples obtained from 83 patients were tested by an immunoenzymometric assay. All patients received corticosteroids and sampling occurred at 6-12-mo intervals during 96 +/- 6 mo of follow-up. Twenty-nine patients (35%) had an abnormal level. In 26 patients, the abnormality was at presentation. In 3 patients, the abnormality developed 11-127 mo later. Two of these patients had primary hepatocellular carcinoma. Serum aspartate aminotransferase levels were higher in patients with an alpha-fetoprotein elevation at presentation (p less than 0.02). After therapy, the alpha-fetoprotein level normalized and patients entering remission had lower levels than at entry (p less than 0.001). alpha-Fetoprotein levels, however, did not correlate closely with serum aspartate aminotransferase levels at entry nor did they distinguish patients with different patterns of histologic activity. Outcomes after therapy were similar in patients with and without alpha-fetoprotein elevation. Three patients (4%) developed primary hepatocellular carcinoma after 113 +/- 26 mo but only 2 had elevated alpha-fetoprotein levels. We conclude that elevation of the alpha-fetoprotein level occurs commonly at presentation. The abnormality frequently resolves after corticosteroid therapy and it does not have prognostic significance. An elevation that occurs after treatment suggests primary hepatocellular carcinoma. 相似文献
105.
Alpha 4 beta 1-integrin expression on sickle reticulocytes: vascular cell adhesion molecule-1-dependent binding to endothelium 总被引:7,自引:4,他引:7
Important complications in sickle cell anemia occur secondary to vascular occlusion, which is postulated to be initiated by interactions of erythrocytes with vascular endothelial cells. In patients with sickle cell anemia, up to 25% of reticulocytes express the alpha 4 beta 1-integrin complex. Furthermore, erythrocytes from patients with sickle cell anemia bind to endothelial cells activated by tumor necrosis factor alpha via (TNF alpha) via interactions between erythrocyte alpha 4 beta 1 and endothelial cell vascular cell adhesion molecule-1 (VCAM- 1). Thus, binding of alpha 4 beta 1-expressing reticulocytes to cytokine-activated endothelial cells may initiate vascular complications in sickle cell anemia and perhaps other hemolytic anemias during episodes of infection and inflammation. 相似文献
106.
Seventy-one patients were randomly allocated to undergo either computer-navigated or conventional arthroplasty. A statistically significant improvement in alignment was seen in the computer-navigated cohort. Five-year functional outcome was assessed using the Knee Society, Short Form-36, Western Ontario and McMaster Universities Osteoarthritis Index, and a patient satisfaction score. At 5 years, 46 patients were available for assessment (24 navigated and 22 conventional knees). No patients had undergone revision. No statistically significant difference was seen in any component of any measure of outcome between navigated and conventional cohorts. Longitudinal data showed function to be well maintained with no difference in functional score between 2 and 5 years in either cohort. Despite achieving better alignment, 5 years postoperatively, the functional outcome with computer-navigated knee arthroplasty appears to be no different to that implanted using a conventional jig-based technique. 相似文献
107.
James S Krinsley Moritoki Egi Alex Kiss Amin N Devendra Philipp Schuetz Paula M Maurer Marcus J Schultz Roosmarijn TM van Hooijdonk Morita Kiyoshi Iain MJ Mackenzie Djillali Annane Peter Stow Stanley A Nasraway Sharon Holewinski Ulrike Holzinger Jean-Charles Preiser Jean-Louis Vincent Rinaldo Bellomo 《Critical care (London, England)》2013,17(2):R37
Introduction
Hyperglycemia, hypoglycemia, and increased glycemic variability have each been independently associated with increased risk of mortality in critically ill patients. The role of diabetic status on modulating the relation of these three domains of glycemic control with mortality remains uncertain. The purpose of this investigation was to determine how diabetic status affects the relation of hyperglycemia, hypoglycemia, and increased glycemic variability with the risk of mortality in critically ill patients.Methods
This is a retrospective analysis of prospectively collected data involving 44,964 patients admitted to 23 intensive care units (ICUs) from nine countries, between February 2001 and May 2012. We analyzed mean blood glucose concentration (BG), coefficient of variation (CV), and minimal BG and created multivariable models to analyze their independent association with mortality. Patients were stratified according to the diagnosis of diabetes.Results
Among patients without diabetes, mean BG bands between 80 and 140 mg/dl were independently associated with decreased risk of mortality, and mean BG bands >140 mg/dl, with increased risk of mortality. Among patients with diabetes, mean BG from 80 to 110 mg/dl was associated with increased risk of mortality and mean BG from 110 to 180 mg/dl with decreased risk of mortality. An effect of center was noted on the relation between mean BG and mortality. Hypoglycemia, defined as minimum BG <70 mg/dl, was independently associated with increased risk of mortality among patients with and without diabetes and increased glycemic variability, defined as CV >20%, was independently associated with increased risk of mortality only among patients without diabetes. Derangements of more than one domain of glycemic control had a cumulative association with mortality, especially for patients without diabetes.Conclusions
Although hyperglycemia, hypoglycemia, and increased glycemic variability is each independently associated with mortality in critically ill patients, diabetic status modulates these relations in clinically important ways. Our findings suggest that patients with diabetes may benefit from higher glucose target ranges than will those without diabetes. Additionally, hypoglycemia is independently associated with increased risk of mortality regardless of the patient''s diabetic status, and increased glycemic variability is independently associated with increased risk of mortality among patients without diabetes.See related commentary by Krinsley, http://ccforum.com/content/17/2/131See related commentary by Finfer and Billot, http://ccforum.com/content/17/2/134 相似文献108.
109.
110.