The Inhibitory Effect of Radix Salviae Miltiorrhizae on Hypoxic Structural Remodeling of Intra－acinarPulmonary Arteries

(席思川)(车东媛)(张婉蓉)ＴｈｅＩｎｈｉｂｉｔｏｒｙＥｆｆｅｃｔｏｆＲａｄｉｘＳａｌｖｉａｅＭｉｌｔｉｏｒｒｈｉｚａｅｏｎＨｙｐｏｘｉｃＳｔｒｕｃｔｕｒａｌＲｅｍｏｄｅｌｉｎｇｏｆＩｎｔｒａ－ａｃｉｎａｒＰｕｌｍｏｎａｒｙＡｒｔｅｒｉｅｓ￥ＸＩＳｉ－ｃ...     

In vivo and in vitro anti-tumour response of selenium-protein polysaccharide extracted from rich selenium Agaricus blazei

In this study, the anti-tumour activity of selenium-protein polysaccharide (SPP), a water extract of the rich selenium Agaricus blazei, was tested both in vivo and in vitro. The results of in vivo experiments show that SPP at doses of 50 and 100 mg/kg inhibits proliferation of implanted Sarcoma 180 by 22 and 37.69%, respectively, and promotes lymphocyte transformation and natural killer (NK) cells activity in tumour bearing mice. During the in vitro experiment, we treated the tumour and non-tumour bearing mice with SPP, and prepared serum treated with SPP (Serum_SPP). The results show that Serum_SPP, whether from tumour or non-tumour bearing mice, significantly inhibits K562 cells proliferation and induces their apoptosis, and also significantly increases caspase-3 activity of K562 cells. However, the difference in anti-tumour activity of Serum_SPP between tumour and non-tumour bearing mice is significantly different (p<0.01). The results, according to the studies both in vivo and in vitro, imply that SPP extracted from rich selenium A. blazei can inhibit growth of implanted Sarcoma 180 and promote lymphocyte transformation and NK cells activity in vivo. Additionally, Serum_SPP can inhibit proliferation and cause apoptotic morphological changes and the fragmentation of internucleosomal DNA, and increase caspase-3 activity of K562 cells in vitro, which indicates that apoptosis of K562 cells induced by Serum_SPP may be related to up-regulation of caspase-3.     

深静脉置管预防阿昔洛韦致静脉炎的效果观察

目的探讨深静脉置管术预防阿昔洛韦致静脉炎的效果。方法随机将60例病毒性脑炎患者分为观察组和对照组各30例。观察组行深静脉置管,对照组采用头皮静脉针行静脉输入阿昔洛韦。结果观察组静脉炎发生率(16.7%)显著低于对照组(93.3%),差异有显著性意义(P<0.01)。结论深静脉置管可明显减少阿昔洛韦所致静脉的损害程度,降低静脉炎的发生率。     

湘西自治州1995～2002年男性劳教人员法定性病监测报告

目的 了解湘西自治州重点人群性病患消长趋势，为性病防治工作提供科学依据。方法 从1995年起，采用统一的表格，每年对湘西州劳教所新入教人员进行一次法定性病监测。结果 三种法定性病平均患病率为14．64％(4．96～26．7％)，以1995年最低，1997年最高，8年未发现HIV／AIDS，患病率以20～39岁的性跃期人群为最高，汉族患病率高于其它民族；化程度越高，患病率越低。结论 男性劳教人员作为性病患病的高危人群，是哨点监测理想的目标人群。     

复方天棘胶囊对老龄鼠血气影响的研究

本研究选用昆明种老龄鼠５０只，随机分为正常对照组、阳性对照组和复方天棘胶囊高、中、低剂量组；每组１０只。观察灌服复方天棘胶囊溶液对实验性老龄鼠血气的影响。结果表明，老龄鼠灌服２．０、１．０和０．５ｇ／ｋｇ体重复方天棘胶囊溶液及５．０ｇ／ｋｇ体重红参水煎液１０天后，复方天棘胶囊各剂量组均有不同程度的升高动脉血氧分压（ＰａＯ＿２）和血氧饱和度（ＳａＯ＿２）的作用；且随剂量的递增，ＰａＯ＿２和ＳａＯ＿２明显升高，其中高剂量组升高ＰａＯ＿２和ＳａＯ＿２的作用最为明显（Ｐ＜０．０１）；红本组ＳａＯ＿２也有明显升高（Ｐ＜０．０１）。提示复方天棘胶囊具有升高ＰａＯ＿２和ＳａＯ＿２的作用。     

实时定量RT-PCR的原理及方法

实时定量RT-PCR广泛应用于定量检测mRAN表达水平,为基础研究、分子药物学和生物技术研究提供了一种有力的方法。该法具有易操作、高通量、敏感性高和特异性强的特点,随着新酶、新探针和新仪器的发展而得到快速的发展。本文将对实时定量RT-PCR的定量原理、仪器应用、探针种类的研究进展以及细胞因子mRNA表达水平检测上的应用作一综述。     

装潢居室空气中挥发性化学物的遗传毒性研究

目的探讨装潢居室空气中挥发性化合物的遗传毒性.方法采用小鼠骨髓嗜多染性红细胞微核试验和小鼠精子畸形试验检测居室装潢后空气中挥发性化合物的遗传毒性.结果该化合物对小鼠呼吸系统有明显的刺激作用,与阴性对照组比较,高、中、低剂量组的微核率、精子畸形率均有显著差异性.结论居室装潢后室内空气中挥发性化合物具有遗传毒性作用.     

Kallikrein 4 expression is up-regulated in epithelial ovarian carcinoma cells in effusions

We immunohistochemically analyzed kallikrein 4 protein (hK4) expression in patients with epithelial ovarian carcinoma (181 malignant effusions and 103 solid carcinoma lesions). Expression of hK4 was also studied in 32 effusions using immunoblotting. Carcinoma cells expressed hK4 in 144 (79.6%) of 181 effusions and 85 (82.5%) of 103 solid tumors. Expression was seen in 51% or more of tumor cells in 70 effusions but often was limited to 5% or fewer cells in solid tumors (P = .009, primary tumors vs effusions; P = .002, metastases vs effusions). Immunoblotting showed hK4 expression in 31 of 32 specimens. Stromal cell hK4 expression, seen in 48 (46.6%) of 103 lesions, was significantly higher in primary tumors than metastases (26/43 vs 22/60, P = .019). hK4 expression in tumor cells was significantly lower in International Federation of Gynecology and Obstetrics stage IV than stage III tumors (P = .004, all lesions; P = .012, primary tumors). hK4 expression in carcinoma cells was associated with longer overall survival (not significant; P = .14, peritoneal effusions). hK4 is expressed widely in ovarian carcinoma; levels in carcinoma cells are highest in effusions, which might be related to loss of stromal contribution and/or altered microenvironment. hK4 expression in carcinoma cells of effusions or solid tumors does not predict survival.