High-performance liquid chromatographic determination of creatinine

A high-performance liquid chromatography (HPLC) method for creatinine has been developed. The method is based on ion-pair chromatography. The method is precise and easy to perform. Recoveries from enriched sera were from 98-105% with a mean of 100.5%. At 28 mumol l-1, 52 mumol l-1 and 487 mumol l-1 within-series standard deviations of 2 mumol l-1, 2 mumol l-1 and 10 mumol l-1, respectively were obtained. At 102 mumol l-1 among-series standard deviation of 6 mumol l-1 was obtained. Comparisons between HPLC and autoanalyser (Jaffé reaction) methods showed that the autoanalyser plasma and serum results, which are known to be sensitive to 'pseudocreatinines', varied between 93-217% of the HPLC results. Comparisons between HPLC, autoanalyser and enzymatic methods showed that the methods agree well for urine samples. The method presented appears suitable as a secondary reference method.     

姚真敏教授临证经验撷英

姚真敏教授临床经验丰富 ,擅长内科及老年病的诊治 ,疗效卓著。本文介绍其对老年痴呆症、中风后遗症、胸痹症、脏躁症、痹症、水疝症等的独到诊治经验。     

外剥内剪切结扎术治疗混合痔160例

本文分析 1 60例混合痔患者 ,采用外剥内剪切结扎术治疗 ,着重介绍了内痔部分处理的优越性 ,认为此方法操作简便 ,病程短 ,大大降低了并发症的发生。     

人事代理制引进人才五年经验回顾

解放军第160医院5年来采取人事代理制方式,引进地方高等院校本科毕业生在院作为职员医师,充实临床第一线技术力量,解决了军队小医院编制小、人才不足的问题,促进了医院人才建设发展,为实现“保障有力“奠定了良好的基础.     

近年来有关经络实质假说的文献综述

通过研读１９９２年以来国内主要期刊发表的有关经验实质假说的文献，并进行分析和归纳，总结出当前的几种主要观点，即神经说、血管神经说、生物物理观和生物化学观等。     

在争议与共识中理性成长

随着流行病学、基础研究和高质量临床试验的深入开展,LDL-C是动脉粥样硬化始动和进展的核心要素已经成为不争的事实.     

Time-dependence of urinary neopterin, a marker of cellular immune activity

Neopterin, a marker of cellular immune system activation, is produced by human macrophages after induction by interferon gamma (secreted by T-lymphocytes) and is eliminated mostly in urine. We have documented the circadian rhythm of urinary neopterin in five healthy young men (about 25 years old), using voidings collected during 48 h at fixed 4-h intervals. We repeated the experiment three times, one week apart. Neopterin was measured by high-performance liquid chromatography (HPLC). We clearly show a peak of the excretion of neopterin in the early morning (around 0630 hours +/- 2 h), with total variability (peak-trough difference) reaching 51%. Neopterin is commonly assayed in urinary fractions, so it is imperative to use urine specimens collected at the same time of day--e.g., the first morning urines--to avoid misinterpretation in follow-up of patients.     

1998-2003年北京地区重症加强治疗病房急性呼吸窘迫综合征的临床流行病学调查

目的调查北京地区重症加强治疗病房(ICU)中急性呼吸窘迫综合征(ARDS)的发病情况、原发病、病死率及其影响因素。方法根据1994年欧美ARDS联席会议提出的ARDS诊断标准,回顾性调查1998年5月—2003年4月北京地区8家三级综合医院ICU中ARDS患者的病因、病死率及影响因素。结果383例ARDS患者占同期ICU收治危重患者的4．5%;脓毒症(21．7%)、肺炎(16．2%)、大手术(13．1%)、重症胰腺炎(12．8%)及多发性创伤(10．7%)是最常见的原发病;ARDS起病距原发病时间为(61．8±43．7)h。ARDS总病死率为52．0%,对病死率分别以年龄(≤39、40～64、≥65岁)、性别(男)进行调整,调整前后的病死率差异均有显著性(P均＜0．05),对急性生理学与慢性健康状况评分系统Ⅱ评分(APACHEⅡ,≤12、13～19、≥20分)进行调整后的病死率5年间差异均无显著性。脓毒性休克(36．2%)与心功能衰竭(20．6%)是主要死亡原因,仅14．6%患者死于呼吸衰竭。结论ARDS是北京地区ICU中常见危重症,死亡率仍较高,且近年来并未下降。