环孢素A对实验大鼠脑缺血治疗作用的组织化学研究

【目的】进一步证实免疫因素在脑缺血病理变化中的作用。【方法】将 6 0只局部脑缺血模型SD大鼠分为缺血 3d ,1周和 2周 3个缺血时间组 ,每组分为生理盐水对照和环孢素A治疗两个部分 ,进行TTC和HE组化染色实验 ,并对结果进行统计处理 (t检验 )。【结果】脑缺血 1周 ,环孢素处理组的组织病理学变化较生理盐水对照组明显减轻 ,脑梗塞体积和死亡的神经元数量在环孢素A处理组分别为 3 2 %和 15 8/ 0 0 6 2 5mm2 ,而生理盐水组则为 5 9%和 2 2 1/ 0 0 6 2 5mm2 ,两组之间有显著差异 (P <0 0 5 )。但在脑缺血 3d和 2周组 ,上述变化两组之间无统计学差异。【结论】环孢素A对实验大鼠的缺血性神经元具有一定保护作用 ,结果提示免疫因素的介入 ,加重了脑缺血性损伤。     

Study on sensitivity of neuroglioma to chemotherapeutic drugs]

In this study, the chemosensitivity of 36 cases' fresh neuroglioma specimens was examined in vitro with MTT assay. The separation method for single tumor cell, the number of planting of cells, and the dosage and acting time of drugs, which have effects on the results of MTT assay, were studied systematically. On the basis of this experiment, we established a screening method for the chemosensitivity of neuroglioma; it is accurate, rapid, and simple. At the same time, the results showed that neuroglioma was more sensitive to the chemotherapeutic drugs Vm26, DDP and MMC, and the sensitivity varied with not only the drugs but also the individuals.     

脑外伤后脑干组织中血管内皮细胞及血浆蛋白分布改变的免疫？…

目的:探讨脑外伤后脑干组织中血脑屏障和神经细胞的病理学改变.方法:采用免疫组化染色显示人体脑干组织中的血管内皮细胞(CD31)和某些血浆蛋白(白蛋白及 IgM).结果:1.在正常脑干组织中, CD31阳性物呈连续的线条状,围成规则的血管腔,其周围无明显间隙;血浆蛋白位于血管内,未见到血浆/蛋白阳性神经细胞或神经纤维.2.脑外伤后,脑干中的 CD31阳性物呈不规则弯曲的小片段状,围成不规则的血管腔,其周围间隙明显增宽;同时可见到白蛋白及IgM阳性神经细胞和神经纤维.结论:脑外伤后,脑干组织中的血脑屏障受到了破坏,而且神经细胞也有不同程度的损伤.     

瞬间反应式二次谐波显像评价正常犬肝脏超声学造影的实验研究

目的：评价瞬间反应二次谐波显像（Ｔｒａｎｓｉｅｎｔ ｒｅｓｐｏｎｓｅ ｓｅｃｏｎｄ ｈａｒｍｏｎｅｃ ｉｍａｇｉｎｇ,ＴＲＳＨＩ）对正常肝脏声学造影的增强效果及动态变化规律,并与常规连续二次谐波显像进行对比分析。方法：６只健康杂种犬 经外周静脉注射氟碳声学造影剂,分别用常规二次谐波显像和瞬间反应式二次谐波显像扫描肝脏。造影效果评价使用视觉评分和视频密度分析。结果：常规二次谐波显像有４次Ｉ级增强、４     

小儿外伤性基底节梗塞与血流变关系

采用血液比粘度计检测了14例小儿外伤性基底节梗塞的血粘度,结果Vb,Vp,HCT,全血高切还原粘度,全血低切还原粘度,纤维蛋白原与对照组无差异;Vb_2,ESR,ESR-K,EAL,ED,红细胞聚集性较对照组增高,差异具有非常显著意义(P<0.01)。     

可吸收内固定治疗松质骨骨折22例

目的 总结可吸收内固定材料自身增强聚丙交脂（ＳＲ－ＰＬＬＡ）的临床应用体会。方法 采用波兰进口ＳＲ－ＰＬＬＡ螺钉、棒、线治疗四肢松质骨骨折２２例,随访摄片观察骨折愈合情况,检查关节活动功能。结果 术后有两侧出现并发症,一例局部少量积液,经２次穿刺抽液加压包扎痊愈;一例患者过早去除石膏功能锻炼,螺钉松动滑出,经手术复位石膏外固定治愈,无感染或过敏等情况发生。结论 掌握可吸收材料的生物力学特点,并选择适当的病例,可以最大限度地发挥可吸收内固定材料的优势。     

Role of ras oncogene in adriamycin resistance in human prostate tumor cells

We have previously isolated a drug-resistant, [PC3(R)], variant of human prostate PC3 cell line, which showed significant resistance (>10-fold) to adriamycin. No known mechanisms of drug resistance were found; however, resistant cells expressed more bcl2, c-myc, and ms oncogenes compared to the sensitive cells. In this study, we found that buthionine sulfoximine (BSO), an inhibitor of gamma-glutamyl-cysteine synthetase, decreased glutathione levels by 80-90% in both cell lines. BSO treatment down-modulated Ras protein only in PC3(R) cells and caused a 4-fold sensitization of PC3(R) cells to adriamycin without affecting PC3(W) cells. Farnesol treatment also inhibited expression of Ras protein and concomitantly reversed adriamycin resistance in PC3(R) cells, indicating that altered levels of ras expression plays an important role in drug resistance in PC3(R) cells.     

Traumatic cervical distraction complicated by delayed reduction due to traumatic vertebral artery pseudo-aneurysm

A 23-year-old male involved in a high-speed collision acquired, among other injuries, a wide fracture distraction at C2/C3. Reduction was delayed by an enlarging vertebral artery pseudo-aneurysm at the site of injury which was successfully treated by proximal endovascular occlusion with a detachable polymer-filled balloon. A traumatic arachnoid cyst or pseudomeningocele was also demonstrated at the injury site but intervention was not required. The treatment and outcome of these three uncommon injuries in the literature is reviewed and discussed. Given the severity of the injury, the absence of severe long-term neurological disability is notable.