Functional and metabolic studies of polymorphonuclear leukocytes in the congenital Pelger-Huet anomaly

Polymorphonuclear leukocytes (PMNL) from two individuals with congenital Pelger-Huet anomaly (PHA) were examined to determine whether functional or metabolic defects accompanied the known morphological abnormality. No abnormalities of the PHA cells, as compared to normal control cells, were found when tested for quantitative leukocyte enzyme activities, nitroblue tetrazolium reduction, hexose monophosphate shunt activity, superoxide production, generation of chemiluminescence, or iodination. The PHA cells, as compared to normal PMNL, demonstrated normal chemotaxis and random migration, as well as bactericidal activity.     

Transient neutropenia induced by transfusion of blood exposed to nylon fiber filters

During the course of granulocyte collection by continuous-flow filtration leukopheresis, an abrupt fall in neutrophil count was noted (mean decrease 77%, range 64%-95%). Neutropenia occurred within 5 min of return of blood exposed to the nylon fiber filters and lasted less than 30 min. Saline exposed to the fibers, withdrawal and reinfusion of whole blood, and heparin did not cause neutropenia. Heparinized blood passed by gravity through isolated filters and reinfused immediately also induced neutropenia (mean decrease 64% +/- 8%, range 11%-19%). Blood anticoagulated with ACD (decrease 19.5% +/- 6%, range 6%-56%), heparinized plasma (N = 10, decrease 15% +/- 3%, range 3%-29%) and platelet-rich plasma exposed to the filters failed to produce neutropenia. 91% +/- 2% of the neutrophils adhered to the fibers using heparinized blood as compared to 21% +/- 5% using ACD (p less than 0.001). All donors were asymptomatic during the infusions. These results suggest that during neutrophil adherence a substance is released which produces profound, transient neutropenia perhaps by inducing margination of cells.     

男护士在妇产科实习体验的质性研究

目的探讨男护士在妇产科的实习体验。方法采用现象学研究方法,深入访谈2012年3月~2013年5月在妇产科实习的男护士12名,并用Colaizzi的7步分析法分析访谈资料。结果男护士在妇产科实习的主要体验有：得不到患者的理解与支持、失落感、学习积极性下降、生活学习不方便、对专科操作不满意等。结论男护士在妇产科实习期间有着多种不良体验,临床带教老师要积极采取措施帮助他们顺利完成在妇产科的实习。     

误诊为药源性肌病的抗合成酶综合征1例

1例42岁男性慢性乙型肝炎患者口服拉米夫定（100mg,1次／d）和阿德福韦酯（10mg,1次／d）治疗,约1个月后出现四肢肌肉酸痛、乏力,双下肢水肿。实验室检查：肌酸激酶9368U／L,肌红蛋白〉4317o,μg／L。肌电图示右侧三角肌肌源性损害。疑为横纹肌溶解症。停用拉米夫定及阿德福韦酯,肌酸激酶下降,肌无力症状好转。1年后,患者再次出现双下肢水肿并腹胀伴间断发热。肌酸激酶5546U／L,肌红蛋白〉1200μg／L,抗Jo-1抗体阳性。诊断：多发性肌炎,抗合成酶综合征。给予保肝、利尿、营养神经等治疗。2周后,加用恩替卡韦0．5mg、1次／d抗病毒治疗。2个月后,给予糖皮质激素治疗。1个月后,患者四肢肌肉酸痛、无力症状基本缓解,复查肌酸激酶正常。     

高频宽景成像超声在战士训练伤中的诊断意义

目的：探讨高频宽景成像超声在战士训练伤中的诊断意义。方法：对驻地120名因训练伤前来就诊的战士进行超声检查,其影像结果均经MR或临床证实。结果：高频超声对液性病变的诊断符合率为100%,具有独特的优势;在肌肉及肌腱的损伤诊断中,符合率分别为77.4%和92.6%。结论：超声检查操作简单,携带方便,在战时、野外或无昂贵检查设备的条件下,超声成为应急诊断的重要手段之一。