Kv1 channels selectively prevent dendritic hyperexcitability in rat Purkinje cells

Purkinje cells, the sole output of the cerebellar cortex, encode the timing signals required for motor coordination in their firing rate and activity pattern. Dendrites of Purkinje cells express a high density of P/Q-type voltage-gated calcium channels and fire dendritic calcium spikes. Here we show that dendritic subthreshold Kv1.2 subunit-containing Kv1 potassium channels prevent generation of random spontaneous calcium spikes. With Kv1 channels blocked, dendritic calcium spikes drive bursts of somatic sodium spikes and prevent the cell from faithfully encoding motor timing signals. The selective dendritic function of Kv1 channels in Purkinje cells allows them to effectively suppress dendritic hyperexcitability without hindering the generation of somatic action potentials. Further, we show that Kv1 channels also contribute to dendritic integration of parallel fibre synaptic input. Kv1 channels are often targeted to soma and axon and the data presented support a major dendritic function for these channels.     

Interleukin-10 gene polymorphisms and susceptibility to brucellosis in Iranian patients

Background: Interleukin-10 (IL-10) is a Th2-type cytokine that inhibits macrophage activation. It is known that production of IL-10 is affected by its gene promoter polymorphisms. Objective: To investigate the relationship between IL-10 gene promoter polymorphisms and susceptibility to brucellosis. Methods: One hundred and ninety patients with brucellosis and 81 healthy animal husbandmen who owned infected animals and consumed their contaminated dairy products were included in this study. All individuals were genotyped for three bi-allelic IL-10 gene promoter polymorphisms at positions -1082(G/A), -819(T/C), and -592(A/C) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The distribution of C alleles at positions -592 and -819 of IL-10 were significantly higher in patients than in the healthy animal husbandmen (p=0.034 and p=0.0086, respectively). IL-10 ATA single and double haplotypes were significantly higher in controls, compared to the patients (p= 0.0278 and p=0.013, respectively). Conclusion: According to the results higher frequency of C alleles at positions -592 and -819 of IL-10 in patients may be considered as genetic factors for susceptibility to brucellosis.     

探讨文明创建对医院发展的作用

全国文明单位称号是国家综合评比一个单位精神文明建设成果的最高褒奖.良好的医院形象是提升医院综合实力、增强其竞争力的重要手段.医院每年制定文明创造重点,明确文明创造的目标、任务和重点,将文明创造工作与业务工作结合起来进行规划、实施、考核和奖惩.医院设有精神文明建设领导小组和办公室,隶属党办,负责精神文明建设的日常协调...     

环丙沙星注射剂常见配伍禁忌

目的：归纳总结环丙沙星注射剂的配伍禁忌。方法：收集2001年1月～2014年6月国内公开发表的关于环丙沙星配伍禁忌的文章,进行总结分析。结果：共有58种药物与环丙沙星注射剂存在配伍禁忌,配伍常出现变色、浑浊、产生沉淀等现象,溶液的pH值、温度、金属离子等会对环丙沙星溶液的稳定性产生影响。结论：环丙沙星注射剂与许多药物存在配伍禁忌,不应与其他药物配伍,临床使用时应选择生产厂家提供或规定的溶剂作溶媒,现配现用,尽量缩短在光线中暴露的时间。     

基于柯氏模型构建执业药师培训效果评价指标体系

目的:探索并构建执业药师培训效果评价指标体系。方法:基于柯氏四级培训评估模型理论,通过文献调研、小组讨论与德尔菲法确定执业药师培训效果评价指标体系的条目,以层次分析法(AHP)计算权重。结果:经过两轮专家函询,最终确定包含4项一级指标、10项二级指标、22项三级指标的执业药师培训效果评价指标体系。结论:本次构建的评价指标体系具有一定的科学性与可靠性,对执业药师的规范化培训考核具有参考作用。     

针刺手法效应规律及神经电信息机制研究

针刺手法是影响针刺疗效的关键,然而其作用规律、机制尚不完全清楚。天津中医药大学实验针灸学研究中心郭义教授团队与天津大学电气自动化与信息工程学院王江教授团队,长期以来围绕针刺手法作用规律及神经电信息机制开展合作研究,构建了常见针刺手法的数学模型,用数学语言解析了针刺手法操作过程,实现了针刺手法的科学表征;揭示了基于神经电信息的针刺手法部分作用规律;刻画了针刺手法电信号的非线性动力学特征,明确不同针刺手法诱发神经电信息编码存在差异,是针刺手法效应差异的机制之一。研究成果为针刺手法的科学性奠定了提供了实验依据,对针刺手法的传承、应用和发展具有促进作用。     

Comparing the effects of 8-week treatment with fluoxetine and imipramine on fasting blood glucose of patients with major depressive disorder

This study was designed to compare the effects of fluoxetine and imipramine on fasting blood glucose (FBG) in patients with major depressive disorder. Sixty nondiabetic patients with major depressive disorder (based on Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition criteria) entered this randomized, double-blind study. Patients did not receive any medication affecting serum FBG levels for at least 2 weeks before the initiation of the study. Patients were assigned to receive 20 to 40 mg/d of fluoxetine or 75 to 200 mg/d of imipramine for 8 weeks. Pregnant women and patients with diabetes mellitus and a history of any major heart disease were excluded from this study. Additionally, none of the patients should have received electroconvulsive therapy within 6 months before the initiation of the antidepressants. FBG levels were measured at the initiation, as well as 4 and 8 weeks after starting antidepressants. Nineteen patients in the fluoxetine and 24 patients in the imipramine groups completed the study. In the fluoxetine group, FBG level was decreased from 88.5 mg/dL (baseline) to 85.0 mg/dL at week 4 (P = 0.73), and to 79.8 mg/dL at week 8 (P < 0.001). On the other hand, in the imipramine group, FBG level was increased from 86.96 mg/dL (baseline) to 89.71 mg/dL at week 4 (P = 0.079), and to 96.90 mg/dL at week 8 (P < 0.001). This 8-week study showed that FBG levels may decrease in depressive patients receiving fluoxetine and may increase in those patients treated with imipramine. Therefore, it is suggested to measure and monitor FBG before initiation and during treatment with fluoxetine and imipramine.     

介孔二氧化硅纳米粒增溶型非诺贝特片剂的制备及体内外研究

目的 制备介孔二氧化硅纳米粒(mesoporous silica nanoparticles,HK)增溶型非诺贝特(fenofibrate,FNB)片剂并进行体内外研究。方法 用吸附法以HK为FNB的载体制成固体分散体(FNB-HK)。采用差示扫描量热法、X射线衍射法和傅里叶红外光谱法分析非诺贝特在FNB-HK中的存在状态。通过考察体外溶出度,优化处方并制片。将自制片和市售片分别对家兔单剂量口服给药,采用高效液相色谱法测定家兔血浆药物浓度。结果 FNB-HK表征表明HK能够抑制非诺贝特的结晶。经过处方优化,乳糖做填充剂,8%羧甲基淀粉钠为崩解剂时,片剂能够达到最理想的溶出速率。自制片与市售片相比,体内达峰时间提前,达峰浓度增大,相对生物利用度为149.95%。结论 本研究研制的片剂能显著改善FNB的溶出速率,提高其口服生物利用度。     

穿心莲内酯对铜绿假单胞菌PAO1生物被膜形成的影响

目的:采用激光共聚焦显微镜(CLSM)技术考察穿心莲内酯对铜绿假单胞菌PAO1生物被膜形成的影响,进一步揭示穿心莲内酯的抗菌机制。方法:使用玻璃爬片生物被膜培养法,在不同浓度穿心莲内酯(10μg/ml、1μg/ml、0.1μg/ml和0.01μg/ml)条件下孵育PAO1,并分别于24h、48h及72h取出玻璃爬片,用硫氰酸荧光素标记的刀豆蛋白和碘化吡啶双重免疫荧光染色,采用激光共聚焦显微镜技术观察穿心莲内酯对铜绿假单胞菌生物被膜形成的影响,并测量生物被膜的厚度。结果:断层图像显示和空白对照组比较,用药后PAO1生物被膜生长不完全,出现多种形态,其中在0.1μg/ml剂量下,生物被膜呈破碎絮状;与空白对照组比较,穿心莲内酯在0.1μg/ml、0.01μg/ml剂量下,可明显影响PAO1生物被膜的生长厚度。结论:穿心莲内酯可能具有干扰铜绿假单胞菌PAO1生物被膜形成的作用。