Survivin蛋白在乳腺癌发生、发展不同阶段的表达及意义

背景与目的:Survivin蛋白是新近发现的凋亡抑制因子,主要通过抑制Caspase-3、Caspase-7而阻断细胞凋亡过程,目前研究表明Survivin蛋白在很多恶性肿瘤组织中高表达。本研究通过观察Survivin在正常乳腺组织、乳腺囊性增生组织、乳腺不典型增生组织及乳腺癌组织中的蛋白表达,探讨Survivin在乳腺癌发生、发展中的作用。方法:采用免疫组织化学SP法检测正常乳腺组织(96例)、乳腺囊性增生组织(56例)、乳腺不典型增生组织(12例)及乳腺癌组织(119例)中Survivin蛋白的表达,并分析其和乳腺癌临床病理特征的关系。结果:Survivin蛋白在正常乳腺组织、乳腺囊性增生组织、乳腺不典型增生组织及乳腺癌组织中的阳性率分别为4.2%(4/96)、5.4%(3/56)、42.7%(5/12)、72.2%(86/119),后二者的阳性率明显高于前二者(P<0.005);浸润性非特殊类型乳腺癌Survivin蛋白阳性率(82.0%,73/89)明显高于浸润性特殊型乳腺癌(45.4%,10/22)和早期浸润性乳腺癌(37.5%,3/8)(P<0.05);Survivin蛋白表达在有淋巴结转移的乳腺癌中有升高的趋势,但差异无统计学意义(P>0.05)。结论:Survivin蛋白在乳腺癌发生、发展不同阶段的表达呈进行性上升的趋势,过度表达提示预后不良。     

Significance of vascular endothelial growth factor expression and its correlation with inducible nitric oxide synthase in gastric cancer

AIM:To investigate the clinical significance of the expression of VEGF165mRNA and the correlation with vascular endothelial growth factor(VEGF) protein and inducible nitric oxide synthase (iNO) in human gastric cancer.METHODS:We tested VEGF165mRNA expression in 31 cases of resected gastric cancer specimens and normal paired gastric mucosae by RT-PCR.Total RNA was extracted with TRIzol reagents,transcribed into cDNA with gligo (dT15) priming,inner controlled with β-actin expression and agarose gel isolated after PCR.VEGF expression was quantitated with IS1000 imaging system.Meanwhile we also examined expression levels of VEGF protein and iNOS in 85 cases of gastric cancer.All paraffin-embedded samples were immunohistochimically stained by streptavidin-peroxidase method (SP).RESULTS:The mean expression of VEGF165mRNA in gastric cancer was 1.125&#177;0.356,significantly higher than that of normal paired mucosea,which was 0.760&#177;0.278.The data indicated that the expression level of VEGF165mRNA was well related to lymph node metastasis and TNM stages of UICC.The expression levels in patients with lymph node metastasis and without lhmph node metastasis were 1.219&#177;0.377 and 0.927&#177;0.205 respectively (p&lt;0.05),The expression in stages Ⅰ,Ⅱ,Ⅲ,Ⅳ was 0.934&#177;0.194,1.262&#177;0.386 respectively (p&lt;0.01).Further analysis showed the lymph node metastasis rate in the group with over-expression of VEGF was higher than that in the group with low expression of VEGF(83.3% vs 46.2%),and the ratio of stage Ⅲ+Ⅳ in the group with over-expression of VEGF was also higher than that in the group with low expression with VEGF (77.8% vs 33.8%)(p&lt;0.05).The positive rates of expression of VEGF protein and iNOS in 85 cases of gastric cancer were 75.4% and 58.8% respectively,and 50.1% of the patients showed positive staining both for iNOS and VEGF,the correlation with the two tactors was significant (p=0.018).But more intensive analysis showed the immunoreactive grades of VEGF were not associated with that of iNOS.CONCLUSIONS:The expression of VEGF165mRNA is well related with lymph node metastasis and TNM stages of UICC in gastric cancer of gastric cancer.The relationship can be observed between the expression of VEGF and iNOS in gastric cancer.