374例高龄男性维生素D补充情况的调查

摘 要 目的:调查高龄男性血清25-羟基维生素D［25（OH）D]的正常情况,分析补充维生素D与血清25（OH）D的关系。方法：对374例高龄男性维生素D补充情况进行调查,并检测其血清25（OH）D,分析两者间的关系。 结果：374例高龄男性中,血清25（OH）D水平正常率仅为10%,其中88例（24%）补充维生素D ,其血清25（OH）D水平为（20.82±9.27） ng·ml^-1,286例（76%）未补充维生素D,其25（OH）D水平为（18.27±8.49 ）ng·ml^-1,差异有统计学意义（P＜0.05）。 结论：高龄男性血清维生素D正常率较低,而补充维生素D可能是提高高龄男性25（OH）D水平的重要方法。     

岩黄连注射液与药材及其提取物间指纹图谱相关性研究

目的:研究岩黄连注射液、岩黄连药材及其提取物指纹图谱的相关性,为建立岩黄连注射液指纹图谱的检测标准提供依据。方法:采用 HPLC 法,Shim-pack CLC-ODS 色谱柱(250mm×6.0mm,5μm),乙腈-缓冲溶液[0.05moL·L~(-1)庚烷磺酸钠-0.05moL·L~(-1)磷酸二氢钾(1:1),含0.2%乙二胺,用磷酸调 pH 3.0]梯度洗脱,流速为1mL·min~(-1),检测波长285nm。以中药色谱指纹图谱相似度评价系统的操作规范(版本2004A)计算。结果:岩黄连注射液与药材及其提取物间指纹图谱均有较好的相关性。结论:本研究为岩黄连注射液指纹图谱的制订提供依据。     

951例心功能不全患者地高辛血清浓度的监测分析

目的: 了解地高辛血清浓度的监测情况.方法:调查深圳市人民医院1998～2000年3 a完成的951例心功能不全患者地高辛血清浓度监测数据,并分析年龄对地高辛血清浓度的影响.结果:地高辛血清浓度在有效范围内比例逐年上升,低于治疗范围的比例较高,高出治疗范围的比例较低;高于治疗范围的患者中毒率为53.6%(52/97),有效浓度内患者中毒率为1.3%(6/470);70岁以上组血清浓度明显高于其他年龄组,年龄与血清浓度超出正常范围的比例间无明显相关性;中毒的临床表现以心律失常(54.2%)和消化道反应(34.7%)为主;中毒原因有血肌酐增高、高血钾、低血钾及伴发感染等.结论:地高辛血清浓度监测能较好地反映地高辛临床疗效,地高辛浓度正常不能排除药物中毒的可能性,老年患者是临床监测的重点.     

应用B超研究儿童甲状腺体积

综述近几年来应用Ｂ超诊断儿童甲状腺肿的方法及测量值研究进展。用Ｂ超测量甲状腺体积是一种精确、客观、且安全、无创伤性的方法。目前,我国８～１０岁碘充足儿童甲状腺体积正常值均低于ＷＨＯ／ＩＣＣＩＤＤ推荐的正常值。因此,ＷＨＯ／ＩＣＣＩＤＤ推荐的儿童甲状腺体积正常值对中国儿童并不适用,有必要重新建立年龄和体表面积分组的碘充足的中国儿童甲状腺体积正常值。     

莫能菌素的毒性及其中毒的临床表现

莫能菌素是一类畜禽专用的抗生素,人类主要通过生产接触和误服导致中毒。主要损害肌肉组织,可出现肝、肾、心等多脏器的损害,严重者可因多脏器功能衰竭而死亡。目前尚无特效解毒药,治疗以清除毒物、对症与支持疗法为主。莫能菌素导致人类中毒案例的文献报告国外十分罕见,至今国内尚未见病例报道。现根据作者实践。结合国外文献做一综述。     

皮下注射丙烯腈对大鼠致畸作用研究

目的　评价丙烯腈对雌性大鼠有无致畸作用。方法　于雌性大鼠妊娠第 7～ 16天皮下注射丙烯腈 (AN) 15 ,2 5 ,35mg·kg-1,常规方法检测AN的胚胎毒性和致畸作用。结果　 2 5mg·kg-1组死胎率、吸收胎率升高 ,胎鼠平均体重、体长、尾长减小 ,与阴性对照组比较差异有显著性 (P <0 0 5 ) ;35mg·kg-1组除窝平均活胎数减少 ,差异有显著性 (P <0 0 5 ) ,其余各指标与阴性对照组比较差异有非常显著性 (P <0 0 1) ;2 5 ,35mg·kg-1组畸胎率、母体畸胎率、活胎畸形率升高 ,与对照组相比差异有显著性和 /或非常显著性 (P <0 0 5或P <0 0 1) ,且有明确的剂量效应或剂量反应关系。本实验皮下注射AN染毒的致畸最低剂量为 15mg·kg-1,致畸指数为 6 3,以骨骼畸形为主 ,外观畸形主要为无尾、短尾等。结论　丙烯腈皮下注射染毒有明显的胚胎毒性 ,致畸剂量小于经口染毒阈剂量 ,是一种弱致畸物。     

Safety of a quadrivalent human papillomavirus vaccine in a Phase 3, randomized,double-blind,placebo-controlled clinical trial among Chinese women during 90 months of follow-up

Background

A quadrivalent human papillomavirus (qHPV) vaccine (HPV6/11/16/18) has demonstrated efficacy and acceptable safety in international studies. However, these studies did not include participants from mainland China, which has a substantial burden of HPV-related disease. This is the first safety report with a follow-up period of up to 90?months from a randomized, double-blind, placebo-controlled trial of qHPV vaccine in Chinese women 20–45?years of age.

不同工艺各类棉酚的反相薄层色谱分析

目的：考察苯胺法与酸法工艺制备的各类棉酚的质量,本胺法工艺制品中是否残留本胺棉酚。方法：采用RP18F反相薄层板,以丙酮-水（4：1）为展开系统分离展开。结果：苯胺法工艺生产的棉酚中出现苯胺棉酚的斑点。结论：首次用反相薄层色谱法确证了苯胺法工艺生产的棉酚中残留有苯胶棉酚。     

Effects of cyclic vs. daily treatment with human parathyroid hormone (1-34) on murine bone structure and cellular activity

Previously, we demonstrated that the human parathyroid hormone (1-34) fragment (hPTH(1-34)) increased bone strength in proportion to its effects on BMD and cortical bone structure in the murine femur by comparing cyclic vs. daily administration of hPTH(1-34). Both cyclic and daily regimens increased vertebral BMD similarly at 7 weeks. Here, we have examined the effects of daily and cyclic PTH regimens on bone structure and cellular activity by static and dynamic histomorphometry. Twenty-week-old, intact female C57BL/J6 mice were treated with the following regimens (n=7 for each group): daily injection with vehicle for 7 weeks [control]; daily injection with hPTH(1-34) (40 microg/kg/day) for 7 weeks [daily PTH]; and daily injection with hPTH(1-34) (40 microg/kg/day) and vehicle alternating weekly for 7 weeks [cyclic PTH]. At days 9 and 10, and 2 and 3 prior to euthanasia, calcein (10 mg/kg) was injected subcutaneously. At the end of study, the lumbar vertebrae 1-3 and the left femora were excised, cleaned, and processed for histomorphometry. In the lumbar vertebrae, daily and cyclic PTH regimens significantly increased cancellous bone volume (BV/TV), trabecular number, trabecular osteoclast and osteoblast perimeters, trabecular mineral apposition rate (MAR) and bone formation rate (BFR), and periosteal MAR and BFR compared to control, with no significant difference between the two PTH-treated groups. Increased trabecular tunneling was observed in both PTH-treated groups. Both regimens tended to increase vertebral cortical bone formation parameters with the effects at the periosteum site being more marked than those at the endosteum site, resulting in a significant increase in cortical width. In the femur, the effects of cyclic PTH on BV/TV, trabecular width and number, trabecular and endocortical osteoblast and osteoclast perimeters, cortical width, and trabecular and periosteal BFR were less marked than those of daily PTH. A cyclic PTH regimen was as effective as a daily regimen in improving cancellous and cortical bone microarchitecture and cellular activity in the murine vertebra.