全文获取类型
收费全文 | 4038篇 |
免费 | 213篇 |
国内免费 | 48篇 |
专业分类
耳鼻咽喉 | 35篇 |
儿科学 | 79篇 |
妇产科学 | 52篇 |
基础医学 | 514篇 |
口腔科学 | 152篇 |
临床医学 | 237篇 |
内科学 | 964篇 |
皮肤病学 | 78篇 |
神经病学 | 333篇 |
特种医学 | 204篇 |
外科学 | 733篇 |
综合类 | 19篇 |
预防医学 | 93篇 |
眼科学 | 84篇 |
药学 | 251篇 |
中国医学 | 4篇 |
肿瘤学 | 467篇 |
出版年
2023年 | 42篇 |
2022年 | 81篇 |
2021年 | 147篇 |
2020年 | 83篇 |
2019年 | 88篇 |
2018年 | 108篇 |
2017年 | 104篇 |
2016年 | 96篇 |
2015年 | 112篇 |
2014年 | 129篇 |
2013年 | 167篇 |
2012年 | 230篇 |
2011年 | 226篇 |
2010年 | 120篇 |
2009年 | 114篇 |
2008年 | 170篇 |
2007年 | 238篇 |
2006年 | 204篇 |
2005年 | 199篇 |
2004年 | 169篇 |
2003年 | 143篇 |
2002年 | 147篇 |
2001年 | 97篇 |
2000年 | 83篇 |
1999年 | 74篇 |
1998年 | 47篇 |
1997年 | 37篇 |
1996年 | 41篇 |
1995年 | 32篇 |
1994年 | 24篇 |
1993年 | 27篇 |
1992年 | 59篇 |
1991年 | 54篇 |
1990年 | 37篇 |
1989年 | 57篇 |
1988年 | 60篇 |
1987年 | 75篇 |
1986年 | 54篇 |
1985年 | 37篇 |
1984年 | 34篇 |
1983年 | 42篇 |
1982年 | 18篇 |
1981年 | 17篇 |
1980年 | 22篇 |
1979年 | 27篇 |
1978年 | 14篇 |
1977年 | 12篇 |
1973年 | 14篇 |
1971年 | 13篇 |
1970年 | 15篇 |
排序方式: 共有4299条查询结果,搜索用时 15 毫秒
91.
Kitamura R Toyoshima T Tanaka H Kawano S Kiyosue T Matsubara R Goto Y Hirano M Oobu K Nakamura S 《Journal of cancer research and clinical oncology》2012,138(8):1299-1310
Purpose
The aim of this study was to confirm the expression profile of cytokeratin (CK)17 in comparison with that of CK13 in oral squamous cell carcinoma (OSCC) and leukoplakia and to clarify an association of CK17 with the OSCC differentiation.Materials
The expression of CK17 and CK13 was immunohistochemically examined in 105 patients with OSCC and 108 patients with leukoplakia. A correlation of CK expression with clinicopathological variables was carried out. The over-expression levels of CK17 mRNA were analyzed by real-time RT-PCR in 5 OSCC cell lines (HSC-2, HSC-3, SAS, SQUU-A, SQUU-B).Results
CK17 and CK13 were detected in 101 (96.2?%) and three (2.9?%) of the 105 OSCCs, respectively. CK17 was significantly expressed in well-differentiated OSCC compared to moderately/poorly differentiated OSCC (p?0.01). As detected in 19 of the 34 dysplastic leukoplakias (55.9?%) and 36 of the 74 hyperplastic leukoplakias (48.6?%), CK17 was significantly expressed in dysplastic leukoplakias (p?0.01). As detected in 11 of the 34 dysplastic (32.4?%) and 52 of the 74 hyperplastic leukoplakias (70.3?%), CK13 was significantly expressed in hyperplastic leukoplakias (p?0.01). The relative expression of CK17 mRNA in HSC-2 was significantly higher than in HSC-3 and SAS (p?0.05). Moreover, the relative expression of CK17 mRNA in SQUU-A was significantly higher than in SQUU-B (p?0.05).Conclusion
CK17 expression could be associated with the differentiation and the malignancy of OSCC. A combination pattern of CK17/CK13 might be a suitable marker of malignant transformation. 相似文献92.
93.
R. Nomura S. Naka H. Nemoto M. Otsugu S. Nakamura T. Ooshima K. Nakano 《Archives of oral biology》2013
Objective
Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA).Method
Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP−/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA−) and MT8148PD (CBP−/PA−), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties.Results
TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was ∼10% and ∼3% in the case of CBP+/PA− strains. Analyses of 200 clinical strains showed the CBP+/PA− group to have higher adhesion and invasion rates than other groups.Conclusions
CBP+/PA− S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis. 相似文献94.
95.
Mori H Kobara H Fujihara S Nishiyama N Kobayashi M Masaki T Izuishi K Suzuki Y 《World journal of gastroenterology : WJG》2012,18(24):3177-3180
Rectal perforations due to glycerin enemas (GE) typically occur when the patient is in a seated or lordotic standing position. Once the perforation occurs and peritonitis results, death is usually inevitable. We describe two cases of rectal perforation and fistula caused by a GE. An 88-year-old woman presented with a large rectal perforation and a fistula just after receiving a GE. Her case was further complicated by an abscess in the right rectal wall. The second patient was a 78-year-old woman who suffered from a rectovesical fistula after a GE. In both cases, we performed direct endoscopic abscess lavage with a saline solution and closed the fistula using an over-the-scope-clip (OTSC) procedure. These procedures resulted in dramatic improvement in both patients. Direct endoscopic lavage and OTSC closure are very useful for pararectal abscess lavage and fistula closure, respectively, in elderly patients who are in poor general condition. Our two cases are the first reports of the successful endoscopic closure of fistulae using double OTSCs after endoscopic lavage of the debris and an abscess of the rectum secondary to a GE. 相似文献
96.
97.
Takeuchi T Tanaka Y Kaneko Y Tanaka E Hirata S Kurasawa T Kubo S Saito K Shidara K Kimura N Nagasawa H Kameda H Amano K Yamanaka H 《Modern rheumatology / the Japan Rheumatism Association》2012,22(3):327-338
We retrospectively investigated the ability of adalimumab (ADA) to reduce disease activity, improve physical function, and retard the progression of structural damage in 167 patients with rheumatoid arthritis. Clinical and functional outcomes were compared between patients with or without prior biologic treatment and those with or without concomitant methotrexate (MTX) treatment. At week 52, 38.3% achieved clinical remission: 42.4 and 28.6% of patients achieved remission in those without and with previous biologics, respectively, while 42.7 and 12.5% of patients achieved remission in those with and without concomitant MTX, respectively. ADA treatment significantly reduced the rate of radiographic progression from 27.1 ± 46.0 (median 13.6; 25th-75th percentiles 8.3 to 28.9) at baseline to 0.8 ± 5.0 (median 0.0; 25th-75th percentiles -0.9 to 2.0) at week 52 (P < 0.0001). Radiographic progression was absent in 59.8% of patients. Sixty adverse events (34.21/100 patient-years) were reported, 16 of which were serious (9.12/100 patient-years). ADA therapy is highly effective for reducing disease activity, improving physical function, and limiting radiographic progression. It is generally safe and well tolerated by Japanese RA patients in routine clinical practice. 相似文献
98.
Hirakawa E Saito K Hirata S Atsumi T Koike T Tanaka Y 《Modern rheumatology / the Japan Rheumatism Association》2012,22(5):769-773
A 16-year-old male with severe thrombocytopenia and progressive multiple organ infarctions was diagnosed as having catastrophic antiphospholipid syndrome (CAPS) complicated with systemic lupus erythematosus, and was successfully treated with combination of anticoagulants, corticosteroids, plasma exchange, and intravenous cyclophosphamide. Antibodies to phosphatidylserine/prothrombin (PS/PT) complex and cardiolipin (CL)/β(2)-glycoprotein?I (β(2)GPI) were simultaneously detected, indicating that the different pathways of both PS/PT and CL/β(2)GPI might be associated with the radical manifestation of CAPS. 相似文献
99.
Iwai S Kabata T Maeda T Kajino Y Ogawa K Kuroda K Tsuchiya H 《Modern rheumatology / the Japan Rheumatism Association》2012,22(5):766-768
We report the case of a 57-year-old woman with hyperostosis around the bilateral acetabulum associated with untreated secondary hypoparathyroidism. She presented with gait disturbance and inability to walk. Radiographs showed abnormal ossification around her hips. We resected the ossifications to improve joint function. One year after surgery, radiographs showed no recurrence of ossification. When radiographs show excessive hyperostosis, it is important to exclude presence of metabolic bone disease. 相似文献
100.
Shintaro Akamoto Seiji Noge Jun Uemura Norikatsu Maeda Minoru Ohshima Hirotaka Kashiwagi Naoki Yamamoto Masao Fujiwara Shinichi Yachida Takehiro Takama Masanobu Hagiike Keiichi Okano Hisashi Usuki Yasuyuki Suzuki 《Surgery today》2013,43(5):580-582
Although extraperitoneal colostomy is often performed to prevent postoperative parastomal hernia formation following an open abdominoperineal resection of lower rectal cancer, it has not been widely employed laparoscopically because of the difficulty associated with the extraperitoneal route. This paper describes a laparoscopic extraperitoneal sigmoid colostomy using the Endo Retract? Maxi instrument. This surgical technique is easy, and helps to prevent the development of parastomal hernias. 相似文献