Expression of the Prothymosin-α Gene as a Prognostic Factor in Lung Cancer

Prothymosin-α (PTα) is known to play a role in cell proliferation, and the PTα mRNA level may reflect the degree of proliferation of tumor cells. It has been reported that PTα mRNA levels are higher in human colon and liver cancer tissues than in the adjacent normal tissues. We examined the mRNA levels of PTα and c-myc in 20 lung cancers, using Bas 2500Mac systems. The PTα and c-myc mRNA levels in lung cancer tissues were higher than those in normal lung tissues; however, the PTα mRNA levels did not correlate with the stage or pathological subtype of the lung cancer and there was no correlation between the expression of PTα and c-myc. PTα mRNA overexpression in lung cancer was correlated with a poor prognosis. Received: October 13, 2000 / Accepted: May 15, 2001     

Oxygen Hyperbaric Pressure Therapy (OHPT) for Decubitus Ulcers

Aim:  This study aims to establish a pressure ulcers model that visualizes the microcirculation, and to examine the participation of ischemia‐reperfusion injury in the pathophysiology of pressure ulcers.
Methods:  An original system composed of a new skinfold chamber and compression device allowed loading quantitative vertical stress to the skin. An intravital microscopic technique enabled direct visualization of the microcirculation in the physiological condition and in response to pressure application. To estimate the effect of ischemia‐reperfusion injury, animals were divided into two groups: the compression‐release group in which the animals received four cycles of compression‐release which consisted of 2 hours of compression followed by 1 hour of pressure release; and the compression alone group in which the animals underwent continuous compression for 8 hours. Functional capillary density was quantified before the compression procedure and on day1 (35 hours) after the first evaluation.
Results and Conclusions:  The cyclic compression‐release procedure significantly decreased functional capillary density as compared to continuous compression, indicating that in our experimental setting repetition of ischemia‐reperfusion cycle more severely damaged the microcirculation than single prolonged ischemic insult. The finding supports the significant contribution of ischemia‐reperfusion injury to the pathophysiology of pressure ulcers at the level of dynamic in vivo microcirculation.     

Endoscopic thoracic sympathectomy for palmar hyperhidrosis: efficacy of T2 and T3 ganglion resection

BACKGROUND: Endoscopic thoracic sympathectomy has been considered an effective treatment for palmar hyperhidrosis. However, the extent of resection has not been determined in terms of efficacy and complications. We compared the efficacy and complications of 2-ganglion and single-ganglion resection in patients with palmar hyperhidrosis. METHODS: From 1995 to 2000, 75 patients underwent resection of thoracic ganglion T2 and T3. From 2000 to 2003, 67 patients underwent resection of only the T2 ganglion. Eighty of the 142 patients (56%) answered a detailed questionnaire, the results of which were analyzed. RESULTS: Gender, age, family history, and distribution of sweating were similar in both groups. Recurrence rates 1 and 2 years after endoscopic thoracic ganglionectomy were between 0% and 3% in T2 and T3 resection, and between 15% and 19% in T2 resection only. In the combined T2 and T3 resection group, 100% of patients noticed compensatory sweating; in T2 resection, 90% of patients noticed compensatory sweating. As for rates of satisfaction, T2 and T3 resection was superior to T2 resection. CONCLUSIONS: High recurrence rates of palmar hyperhidrosis after single-ganglion resection are reported in the present study. Two-ganglion resection is a superior surgical method to prevent recurrence of palmar hyperhidrosis.     

预切开内镜黏膜切除术治疗结直肠侧向发育型肿瘤的指征分析（含视频）

目的探讨预切开内镜黏膜切除术(pre-cut-endoscopic mucosal resection,pre-cut-EMR)治疗结直肠侧向发育型肿瘤(laterally spreading tumors,LSTs)的临床指征。方法回顾性分析2014年1月—2019年6月在无锡市第二人民医院和复旦大学附属中山医院接受pre-cut-EMR治疗的结直肠LSTs患者临床资料,研究分析病灶临床特征与手术成功率及并发症的关系。结果共132例结直肠LSTs病变纳入研究,LSTs内镜分型中颗粒均一型29例（22.0%）,结节混合型43例（32.6%）,扁平隆起型58例（43.9%）,假凹陷型2例（1.5%）。LSTs病灶直径（2.3±1.5）cm（2.0～5.0 cm）。病灶位于直肠36例(27.3%),乙状结肠15例(11.4%),降结肠10例(7.6%),结肠脾曲17例(12.9%),横结肠21例(15.9%),结肠肝曲24例(18.2%),升结肠6例 (4.5%),盲肠3例(2.3%)。LSTs病理结果：低级别上皮内瘤变58例(43.9%),高级别上皮内瘤变69例(52.3%),黏膜内癌2例(1.5%),癌变3例(2.3%)。132例LSTs病灶应用pre-cut-EMR治疗,手术时间（25.3±13.6）min（20～65 min）,病灶整块切除率95.5%(126/132),完整切除率100.0%（132/132）。术中穿孔2例（1.5%）,均位于乙状结肠,病灶直径分别为4.0 cm、4.5 cm。术中即刻出血12例（9.0%),术后迟发出血2例(1.5%)。术后随访6～24个月,术后创面愈合良好,随访期间病灶未见残留及复发。结论内镜下应用pre-cut-EMR治疗直径<4.0 cm的结直肠LSTs病变是安全有效的。     

复合ADSCs/β-TCP组织工程骨与PRF修复下颌骨缺损的实验研究

目的:探讨脂肪干细胞/β-磷酸三钙(ADSCs/β-TCP)组织工程骨复合富血小板纤维蛋白(PRF)在修复下颌骨缺损中的作用。方法:新西兰大白兔27只,采用随机对照动物实验,随机分为3组,每组9只,培养家兔自体ADSCs,制备自体PRF。在每只兔子的双侧下颌骨制备下颌骨缺损模型。A组:将单纯的β-磷酸三钙(β-TCP)植入双侧下颌骨缺损区,B组:将ADSCs/β-TCP植入双侧下颌骨缺损区,C组:将ADSCs/β-TCP与PRF植入双侧下颌骨缺损区。分别于术后2、4、8周分别处死9只大白兔,并通过大体观察、X线片、以及组织切片观察下颌骨缺损的修复情况。结果:C组在2、4、8周3个时间点的成骨情况明显优于其他2组(P<0.01)。A组与B组之间的成骨情况差异无显著性(P>0.05)。结论:ADSCs/β-TCP复合物与PRF联合应用可促进下颌骨缺损的修复。     

A case of acquired smooth muscle hamartoma of the scrotum

We report a case of acquired smooth muscle hamartoma (ASMH) of tunica dartos, believed to be only the fifth so far reported. A 24-year-old man had a 6-month history of an asymptomatic tight and thickened scrotum. The skin was difficult to pinch. A biopsy specimen from the skin showed increased and proliferated smooth-muscle bundles composed of well-differentiated and uniform spindle cells that showed typical features of acquired smooth muscle hamartoma. Interestingly, dilatations of the lymph vessels were noted in the upper dermis above the proliferated smooth muscles. It has been reported recently that long-standing severe lymphoedema may cause histological features mimicking ASMH. As the present case was not preceded by oedema of the scrotum, we consider this case to be true ASMH.     

结核感染若干年后特异性γ-干扰素应答减弱

假设：随着时间的流逝，结核感染者记忆性T细胞对特异性抗原的应答减弱。背景：很多证据表明特异性全血γ-干扰素（IFN-γ）检测试验Quanti—FERON～TB Gold（QFT—G）能够检测出近期结核感染者，并具有较高的灵敏度和特异性。目的：我们应用此项技术检测日本一个社区的成人群体以确定其流行病学应用价值。方法：1559例参加定期体检的健康志愿者入选本研究。结果：40～49岁人群QFT—G的阳性率为3．1％，50～59岁人群的阳性率为5．9％，60～69岁人群的阳性率为9．8％。作者根据一系列研究估计40～49岁、50～59岁、60～69岁的预期结核感染率分别为11．1％、29．6％、53．1％。预期阳性率与实际阳性率之间存在的巨大差异提示结核感染后随着时间的延长IFN-γ应答减弱。那些经胸部X线证实存在陈旧性结核病灶者阳性率大大低于100％。结论：特异性IFN—γ应答可能在结核感染后随着时间的推移大大地减弱。需要在感染者中长期地纵向研究细胞介导的免疫动力学。