不同诱导方法诱导家蝇幼虫血淋巴中抗菌肽差异性的SDS-PAGE检测

目的 检测不同诱导方法(针刺,紫外,热诱导)诱导家蝇三龄幼虫血淋巴中的抗茵肽的差异性.方法 用不同诱导方法诱导家蝇三龄幼虫,提取血淋巴后,采用梯度乙腈洗脱,应用SDS-PAGE检测抗菌肽的差异性.结果 针刺与未诱导组无明显差异,热诱导组中30%乙腈洗脱组分中在30～71 KD中出现3个差异条带,紫外诱导组50%乙腈洗脱组分在16～20 KD中出现2个差异条带,60%乙腈洗脱组分中在16-20 KD中出现2个差异条带.结论 综合幼虫的成活率及操作的方便性,最佳的诱导方法为热诱导.     

Antiarthritic activity of an orally active C5a receptor antagonist against antigen‐induced monarticular arthritis in the rat

Objective

To determine if the new, orally active C5a receptor antagonist, the cyclic peptide AcF‐[OPdChaWR], reduces the severity of pathology in a rat model of immune‐mediated monarticular arthritis.

Methods

Arthritis was induced in the right knee of previously sensitized rats by the intraarticular injection of methylated bovine serum albumin. Rats were examined for either 14 days or 28 days, or for 49 days following a second antigen challenge at 28 days. The C5a antagonist (1 or 3 mg/kg/day) and/or ibuprofen (30 mg/kg/day) were administered orally on a daily basis either before or after arthritis induction.

Results

Rats receiving AcF‐[OPdChaWR] had significant reductions in right knee swelling, gait disturbance, lavaged joint cell numbers, and right knee histopathology, as well as in serum levels of tumor necrosis factor α (TNFα) and intraarticular levels of interleukin‐6 and TNFα on day 14. In the 14‐ and 28‐day studies, ibuprofen resulted in a similar reduction in gait abnormalities and intraarticular inflammatory cells compared with the C5a antagonist, but was less effective in reducing knee swelling over the course of the study and had no effect on knee histopathology. Combination therapy with AcF‐[OPdChaWR] and ibuprofen resulted in no greater efficacy than with the C5a antagonist alone. Rats injected twice with the antigen in the 49‐day study displayed the most severe histopathology and this, as well as knee swelling and gait abnormalities, was significantly reduced by repeated treatment with the C5a antagonist.

Conclusion

An agent that inhibits the action of C5a in this model significantly reduced joint pathology, while ibuprofen was not effective. C5a antagonists could therefore have broader therapeutic benefits than nonsteroidal antiinflammatory drugs as antiarthritic agents for rheumatoid arthritis.

     

注射用尤瑞克林治疗急性脑梗死的临床疗效观察

目的 观察尤瑞克林治疗急性脑梗死的临床疗效.方法 将符合入选条件的60例急性脑梗死惠者分为治疗组和对照组,每组30例.两组基础治疗相似,治疗组患者加用尤瑞克林,每日1次;分别给予治疗前后神经功能缺损评分和临床疗效比较,同时检测血常规、尿常规、肝肾功能及凝血功能等.结果 治疗组神经功能缺损评分较治疗前差异有统计学意义(P<0.05),疗效明显优于对照组,且无不良反应.结论 尤瑞克林治疗急性脑梗死疗效确切,安全可靠.