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81.
Saori Ichikawa Toshiro Takai Tomoe Yashiki Seizo Takahashi Ko Okumura Hideoki Ogawa Daisuke Kohda Hideki Hatanaka 《Genes to cells : devoted to molecular & cellular mechanisms》2009,14(9):1055-1065
Lipid-binding properties and/or involvement with host defense are often found in allergen proteins, implying that these intrinsic biological functions likely contribute to the allergenicity of allergens. The group 2 major mite allergens, Der f 2 and Der p 2, show structural homology with MD-2, the lipopolysaccharide (LPS)-binding component of the Toll-like receptor (TLR) 4 signalling complex. Elucidation of the ligand-binding properties of group 2 mite allergens and identification of interaction sites by structural studies are important to explore the relationship between allergenicity and biological function. Here, we report a ligand-fishing approach in which His-tagged Der f 2 was incubated with sonicated stable isotope-labelled Escherichia coli as a potential ligand source, followed by isolation of Der f 2-bound material by a HisTrap column and NMR analysis. We found that Der f 2 binds to LPS with a nanomolar affinity and, using fluorescence and gel filtration assays that LPS binds to Der f 2 in a molar ratio of 1 : 1. We mapped the LPS-binding interface of Der f 2 by NMR perturbation studies, which suggested that LPS binds Der f 2 between the two large β-sheets, similar to its binding to MD-2, the LPS-binding component of the innate immunity receptor TLR4. 相似文献
82.
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84.
Noriko Yoshimura Shigeyuki Muraki Hiroyuki Oka Akihiko Mabuchi Yoshio En-Yo Munehito Yoshida Akihiko Saika Hideyo Yoshida Takao Suzuki Seizo Yamamoto Hideaki Ishibashi Hiroshi Kawaguchi Kozo Nakamura Toru Akune 《Journal of bone and mineral metabolism》2009,27(5):620-628
Musculoskeletal diseases, especially osteoarthritis (OA) and osteoporosis (OP), impair activities of daily life (ADL) and
quality of life (QOL) in the elderly. Although preventive strategies for these diseases are urgently required in an aging
society, epidemiological data on these diseases are scant. To clarify the prevalence of knee osteoarthritis (KOA), lumbar
spondylosis (LS), and osteoporosis (OP) in Japan, and estimate the number of people with these diseases, we started a large-scale
population-based cohort study entitled research on osteoarthritis/osteoporosis against disability (ROAD) in 2005. This study
involved the collection of clinical information from three cohorts composed of participants located in urban, mountainous,
and coastal areas. KOA and LS were radiographically defined as a grade of ≥2 by the Kellgren–Lawrence scale; OP was defined
by the criteria of the Japanese Society for Bone and Mineral Research. The 3,040 participants in total were divided into six
groups based on their age: ≤39, 40–49, 50–59, 60–69, 70–79, and ≥80 years. The prevalence of KOA in the age groups ≤39, 40–49,
50–59, 60–69, 70–79, and ≥80 years 0, 9.1, 24.3, 35.2, 48.2, and 51.6%, respectively, in men, and the prevalence in women
of the same age groups was 3.2, 11.4, 30.3, 57.1, 71.9, and 80.7%, respectively. With respect to the age groups, the prevalence
of LS was 14.3, 45.5, 72.9, 74.6, 85.3, and 90.1% in men, and 9.7, 28.6, 41.7, 55.4, 75.1, and 78.2% in women, respectively.
Data of the prevalence of OP at the lumbar spine and femoral neck were also obtained. The estimated number of patients with
KOA, LS, and L2–L4 and femoral neck OP in Japan was approximately 25, 38, 6.4, and 11 million, respectively. In summary, we
estimated the prevalence of OA and OP, and the number of people affected with these diseases in Japan. The ROAD study will
elucidate epidemiological evidence concerning determinants of bone and joint disease. 相似文献
85.
Lymphatic Microvessel Density is an Independent Prognostic Factor in Colorectal Cancer 总被引:5,自引:0,他引:5
Matsumoto K Nakayama Y Inoue Y Minagawa N Katsuki T Shibao K Tsurudome Y Hirata K Nagata N Itoh H 《Diseases of the colon and rectum》2007,50(3):308-314
Purpose Although lymph node metastasis via lymphatic vessels often is related with an adverse outcome, it is not well known whether lymphatic spread to lymph node needs
the development of the new lymphatic formation. In addition, the correlation between lymphangiogenesis and prognosis has not
been well documented. This study was designed to assess the prognostic value of lymphangiogenesis and lymphatic vessel invasion
in colorectal cancer.
Methods We examined 106 colorectal cancer specimens by immunostaining for podoplanin, lymphatic endothelial specific marker. We evaluated
lymphangiogenesis, as measured by lymphatic microvessel density, and lymphatic vessel invasion. We next investigated the association
of these two parameters with the clinicopathologic findings and prognosis.
Results A significant correlation was observed between high lymphatic microvessel density and positive lymphatic vessel invasion (P = 0.0003). Positive lymphatic vessel invasion was significantly associated with the presence of lymph node metastasis (P = 0.0071). The survival curves demonstrated that both high lymphatic microvessel density and positive lymphatic vessel invasion
were correlated with an adverse outcome (P = 0.0004 and P = 0.009, respectively). In a univariate analysis, high lymphatic microvessel density and positive lymphatic vessel invasion
were negatively associated with the overall survival (P = 0.0011 and P = 0.0118, respectively). Furthermore, high lymphatic microvessel density, but not lymphatic vessel invasion, correlated with
a poor outcome in a multivariate analysis (P = 0.0114).
Conclusions Our data suggested that lymphatic vessel invasion was related with lymph node metastasis and that both lymphatic microvessel
density and lymphatic vessel invasion were related with an adverse outcome in colorectal cancer. Furthermore, lymphatic microvessel
density may be a useful prognostic factor in colorectal cancer.
*Deceased.
The Poster presentation at the meeting of the Japanese Society of Gastroenterology, Sapporo, Japan, October 11 to 14, 2006.
Reprints are not available. 相似文献
86.
87.
Minagawa M Yamamoto J Kosuge T Matsuyama Y Miyagawa S Makuuchi M 《Archives of surgery (Chicago, Ill. : 1960)》2007,142(3):269-76; discussion 277
HYPOTHESIS: Although several staging systems for colorectal liver metastasis have been proposed, simple and generally accepted staging systems are not available for this disease. We hypothesized that more detailed analysis of primary colorectal cancer may make it possible to develop a simple staging system and that its stratification ability may be demonstrated by validation against data from unrelated patients. DESIGN: Retrospective analysis of prospectively documented data, development of a stage, and validation against an unrelated cohort. SETTING: Four tertiary referral centers. PATIENTS: Twenty-two clinicopathologic factors were examined in 369 consecutive patients who underwent curative resection for liver metastasis from colorectal cancer (original cohort). Using the independent prognostic factors, a simplified staging system was developed and was validated by data from 229 unrelated patients (validation cohort). MAIN OUTCOME MEASURES: Kaplan-Meier survival curve analyses between different prognostic groups in the cohorts. RESULTS: Multivariate analysis revealed several independent prognostic variables, including hepatic lymph node metastasis (relative risk 4.39), 4 or more colorectal lymph node metastases (RR 1.50), carcinoembryonic antigen level of 50 ng/mL or higher (RR 1.29), and multiple hepatic metastases (RR 1.27). Patients with hepatic lymph node metastasis were assigned to stage 4, and the remaining patients were divided according to number of factors: none, stage 1; 1, stage 2; 2 or 3, stage 3. In the original cohort, median survival in stages 1, 2, 3, and 4 was 7.2, 3.5, 2.0, and 1.3 years, respectively. In the validation cohort, these values were 9.6, 4.1, 2.8, and 1.6 years, respectively. CONCLUSIONS: The proposed simplified staging system was easy to use, was highly predictive of patient outcome, and permitted categorization of patients into treatment groups. Although we validated this staging system, further validation and improvements are needed. 相似文献
88.
Minagawa T Nishizawa S Kamigaito M Nakayama T Okaneya T 《Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology》2007,98(5):723-726
We report a 68-year-old woman who had bilateral renal cell carcinoma (RCC) associated with von Hippel-Lindau (VHL) disease. Surgical resection of a central nervous system hemangioblastoma had been done previously. This time, synchronous bilateral RCCs were found in her kidneys, with metastases to lungs and liver. Right radical nephrectomy was performed to remove the primary tumor in the right kidney. Histopathological examination of the tumor revealed clear cell RCC with a sarcomatoid component. After surgery, transcatheter arterial embolization was performed for the tumor in the left kidney and interferon therapy was commenced. The left renal tumor decreased in size and interferon therapy was effective against the metastatic lung tumors. However, 4 years after resection of the right RCC, the tumor in the left kidney increased progressively in size and partial left nephrectomy was performed. Histopathological examination of the resected tumor also showed clear cell type RCC with a sarcomatoid component. The patient eventually died of her disease at 5 years after resection of the right RCC. RCC associated with VHL is usually of the clear cell type has a relatively good prognosis. Sarcomatoid RCC is rare in VHL patients and, to our knowledge, the present report is the first case of sarcomatoid RCC associated with VHL in the Japanese literature. 相似文献
89.
Hattori H Yamano T Hayashi K Osawa M Kondo K Aihara M Haginoya K Hamano S Izumi T Kaneko K Kato I Matsukura M Minagawa K Miura T Ohtsuka Y Sugai K Takahashi T Yamanouchi H Yamamoto H Yoshikawa H 《Brain & development》2008,30(8):504-512
We evaluated the usefulness of intravenous lidocaine therapy for managing of status epilepticus (SE) during childhood in a retrospective multi-institutional study. Questionnaires were sent to 28 hospitals concerning patients admitted for SE who were managed with lidocaine, assessing patient characteristics, treatment protocols and efficacy. In 279 treated patients, 261 SE occurrences at ages between 1 month and 15 years were analyzed. SE was classified as showing continuous, clustered, or frequently repeated seizures. Considering efficacy and side effects in combination, the usefulness of lidocaine was classified into six categories: extremely useful, useful, slightly useful, not useful, associated with deterioration, or unevaluated. In 148 SE cases (56.7%), lidocaine was rated as useful or extremely useful. Multivariate analysis indicated lidocaine was to be useful in SE with clustered and frequently repeated seizures, and SE attributable to certain acute illnesses, such as convulsions with mild gastroenteritis. Efficacy was poor when SE caused by central nervous system (CNS) infectious disease. Standard doses (approximately 2mg/kg as a bolus, 2mg/kg/h as maintenance) produced better outcomes than lower or higher doses. Poor responders to the initial bolus injection of lidocaine were less likely to respond to subsequent continuous infusion than good initial responders. We recommend lidocaine for use in SE with clustered or frequently repeated seizures, and in SE associated with benign infantile convulsion and convulsions with mild gastroenteritis. Lidocaine should be initiated with a bolus of 2mg/kg. If SE is arrested by the bolus, continuous maintenance infusion should follow; treatment should proceed to different measures when SE shows a poor response to the initial bolus of lidocaine. 相似文献
90.
Mohammed A. Abdel-Ghani Yasuyuki Abe Tomoyoshi Asano Seizo Hamano Hiroshi Suzuki 《Reproductive Medicine and Biology》2011,10(1):43-49