全文获取类型
收费全文 | 2581篇 |
免费 | 136篇 |
国内免费 | 36篇 |
专业分类
耳鼻咽喉 | 33篇 |
儿科学 | 69篇 |
妇产科学 | 32篇 |
基础医学 | 356篇 |
口腔科学 | 202篇 |
临床医学 | 172篇 |
内科学 | 520篇 |
皮肤病学 | 62篇 |
神经病学 | 228篇 |
特种医学 | 93篇 |
外科学 | 247篇 |
综合类 | 69篇 |
一般理论 | 2篇 |
预防医学 | 306篇 |
眼科学 | 37篇 |
药学 | 235篇 |
中国医学 | 16篇 |
肿瘤学 | 74篇 |
出版年
2023年 | 21篇 |
2022年 | 48篇 |
2021年 | 89篇 |
2020年 | 72篇 |
2019年 | 69篇 |
2018年 | 68篇 |
2017年 | 61篇 |
2016年 | 68篇 |
2015年 | 84篇 |
2014年 | 117篇 |
2013年 | 133篇 |
2012年 | 175篇 |
2011年 | 176篇 |
2010年 | 102篇 |
2009年 | 110篇 |
2008年 | 161篇 |
2007年 | 158篇 |
2006年 | 133篇 |
2005年 | 108篇 |
2004年 | 99篇 |
2003年 | 65篇 |
2002年 | 65篇 |
2001年 | 46篇 |
2000年 | 39篇 |
1999年 | 25篇 |
1998年 | 46篇 |
1997年 | 44篇 |
1996年 | 44篇 |
1995年 | 23篇 |
1994年 | 23篇 |
1993年 | 24篇 |
1992年 | 14篇 |
1991年 | 15篇 |
1990年 | 17篇 |
1989年 | 27篇 |
1988年 | 14篇 |
1987年 | 23篇 |
1986年 | 14篇 |
1985年 | 35篇 |
1984年 | 15篇 |
1983年 | 11篇 |
1982年 | 7篇 |
1981年 | 9篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1975年 | 5篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1970年 | 5篇 |
1966年 | 4篇 |
排序方式: 共有2753条查询结果,搜索用时 15 毫秒
101.
T. Capela I. Tavares P. Pereira E. Vigia R. Perdigoto E. Barroso P. Marcelino 《Transplantation proceedings》2014
This study evaluated the relationship between intraoperative hemodynamic instability (IOHI) and the development of calcineurin inhibitor (CNI) toxicity in the early postoperative period after liver transplantation (LT). Eighty-two patients were enrolled during a 1-year period and a 3-month follow-up. IOHI, requiring continuous infusion of vasopressors, was observed in 31 patients (38%, group 1; control group 2, n = 51). Acute kidney injury (AKI) developed in 28 patients (52% in group 1 vs 24% in group 2, P = .02), and CNI-related neurotoxicity (CNI-NT) in 26 (48% in group 1 vs 22% in group 2, P = .03). Group 1 patients received mainly deceased donor grafts (87% vs 57% in group 2, P < .001). An independent association between IOHI and CNI-NT (P = .029) and AKI (P = .016) was observed. The receiver-operator characteristic curve revealed an area under the curve of 0.63 for IHI (sensitivity 56%; specificity 75%) and 0.65 for AKI (sensitivity 56%; specificity 70.2%). In conclusion, patients undergoing LT with IOHI may be more prone to developing CNI-NT and AKI in the early postoperative period. 相似文献
102.
Alexandre de Matos Soeiro Felipe Louren?o Fernandes Rafael Plens Teixeira Pedro Felipe Gomez Nicz Maria Carolina Feres de Almeida Soeiro Carlos V. Serrano Jr. Múcio Tavares de Oliveira Jr. 《Brazilian Journal Of Cardiovascular Surgery》2014,29(2):285-288
Insertion of inferior vena cava filters has been well established in literature,
reducing occurrence of pulmonary embolism after an episode of deep venous thrombosis
in patients with contraindication to anticoagulation. There are a small number of
complications related to procedure and embolization is rare. In this context, we
described a case of intracardiac embolization associated with cardiac tamponade. 相似文献
103.
Daniel Tavares REZENDE Fernando A. M. HERBELLA Luciana C. SILVA Sebasti?o PANOCCHIA-NETO Marco G. PATTI 《Brazilian archives of digestive surgery》2014,27(3):182-183
Background
The upper esophageal sphincter is composed of striated muscle. The stress of intubation and the need to inhibit dry swallows during an esophageal manometry test may lead to variations in basal pressure of this sphincter. Upper esophageal sphincter is usually only studied at the final part of the test. Was observed during the performance of high resolution manometry that sphincter pressure may vary significantly over the course of the test.Aim
To evaluate the variation of the resting pressure of the upper esophageal sphincter during high resolution manometry.Methods
Was evaluated the variation of the basal pressure of the upper esophageal sphincter during high resolution manometry. Were reviewed the high resolution manometry tests of 36 healthy volunteers (mean age 31 years, 55% females). The basal pressure of the upper esophageal sphincter was measured at the beginning and at the end of a standard test.Results
The mean time of the test was eight minutes. The basal pressure of the upper esophageal sphincter was 100 mmHg at the beginning of the test and 70 mmHg at the end (p<0.001). At the beginning, one patient had hypotonic upper esophageal sphincter and 14 hypertonic. At the end of the test, one patient had hypotonic upper esophageal sphincter (same patient as the beginning) and seven hypertonic upper esophageal sphincter.Conclusion
A significant variation of the basal pressure of the upper esophageal sphincter was observed in the course of high resolution manometry. Probably, the value obtained at the end of the test may be more clinically relevant. 相似文献104.
A.T. Nunes L. PereiraA. Cerqueira M. BustorffS. Sampaio I. FerreiraI. Tavares J. SantosM. Pestana 《Transplantation proceedings》2014
Introduction
Renal transplantation (RT) in patients infected with human immunodeficiency virus (HIV) has significantly improved under the advent of combined antiretroviral therapy (cART). The authors describe their experience in RT in patients with HIV from September 2010 to June 2013.Cases Report
Four patients underwent transplantation (3 with HIV-1 and 1 with HIV-2), three patients were male, and one was black. None were coinfected with hepatitis B virus (HBV) or hepatitis C virus (HCV). Etiology of kidney disease was HIV-associated nephropathy (2 patients), immunoglobulin (Ig)A nephropathy, and unknown. Average age at RT was 51 (range, 41–63) years. No patient was of high immunologic risk. Immunosuppression consisted of basiliximab for induction and prednisolone, tacrolimus (TAC), and mycophenolate mofetil for maintenance. TAC levels varied considerably in the early days (8.5–46 ng/mL), requiring major adjustments in TAC dose. Only the HIV-2 patient had delayed graft function. The follow-up of patients with HIV-1 was 37, 19, and 16 months, and 3 months for the HIV-2 patient. CD4+ T cells decreased in the early days after transplantation with subsequent improvement, along with persistent virological suppression. In the HIV-1 group there were no major infectious, cardiovascular, or neoplastic complications. Nevertheless, the HIV-2 patient died 3 months after RT due to H1N1 pneumonia complicated by pulmonary aspergillosis. Average estimated (CKD- EPI) glomerular filtration rate (eGFR) at 6 months was 85.6 mL/min/1.73 m2.Conclusion
Besides the difficulty in adjusting calcineurin inhibitors levels due to its interaction with antiretroviral therapy, namely with protease inhibitors, no patient had acute rejection. Furthermore, all patients presented an excellent control of viro-immunologic parameters. At the last follow-up neither cardiovascular events nor neoplastic complications were observed. Our results highlight the favorable outcome of RT in HIV-1–infected patients. The HIV-2 patient died due to severe infection, and the clinical management and potential benefit of RT in HIV-2–infected patients needs further study. 相似文献105.
Gardênia Matos Paraguassú Milena Góes da Guarda Flávia Calo Aquino Xavier Maria Cristina Teixeira Cangussu Tânia Tavares Rodriguez Maria José Pedreira Ramalho Antônio Luis Barbosa Pinheiro Luciana Maria Pedreira Ramalho 《Lasers in medical science》2014,29(2):773-779
The aim of this study was to assess morphometrically and histologically, the effects of light-emitting diode (LED) (λ630?±?20 nm) phototherapy on reepithelialization and wound contraction during tissue repair in hypothyroid rats. Thyroid hormone deficiency has been associated with disorders of tissue repair. LED phototherapy has been studied using several healing models, but their usefulness in the improvement of hypothyroidism wound healing remains unknown. Under general anesthesia, a standard surgical wound (1 cm2) was produced on the dorsum of 48 male Wistar rats divided into four groups of 12 animals each: EC—control euthyroid, ED—euthyroid + LED, HC—control hypothyroid, and HD—Hypothyroid + LED. The irradiation started immediately after surgery and was repeated every other day for 7 and 14 days. Photographs of the wound were taken at the day of the surgical procedure and on days 8 and 15 after surgery, when animals’ deaths occurred. The specimens were removed, routinely processed, and stained with hematoxylin/eosin. Seven days after the surgery, it was possible to observe statistically significant reductions in the wound area of the irradiated euthyroid group, in comparison to hypothyroid group, irradiated and non-irradiated (ANOVA, p?<?0.05). The reepithelialization was significantly higher in the euthyroid and hypothyroid groups irradiated with LED than in the non-irradiated groups (Fisher’s test, p?<?0.05). No significant difference was found in the experimental period of 14 days among the groups. The hypothyroidism delayed wound healing and the LED phototherapy, at these specific parameters, improved the process of reepithelialization in the presence of hypothyroidism. 相似文献
106.
107.
108.
Rapid prenatal diagnosis of beta thalassemia using DNA amplification and nonradioactive probes 总被引:3,自引:0,他引:3
We used in vitro DNA amplification by the polymerase chain reaction and nonradioactive probes for prenatal diagnosis of beta thalassemia in Chinese from the Guangdong province. Exact molecular diagnoses were made in all 20 fetuses studied over a 6-month period. We conclude that this method of prenatal diagnosis for beta thalassemia is a viable approach in many parts of the world where this disease is common. 相似文献
109.
110.
Marisa I. S. Mendes Henrique G. Colaço Desirée E. C. Smith Rúben J. J. F. Ramos Ana Pop Silvy J. M. van Dooren Isabel Tavares de Almeida Leo A. J. Kluijtmans Mirian C. H. Janssen Isabel Rivera Gajja S. Salomons Paula Leandro Henk J. Blom 《Journal of inherited metabolic disease》2014,37(2):245-254
A reduced response of cystathionine beta-synthase (CBS) to its allosteric activator S-adenosylmethionine (SAM) has been reported to be a cause of CBS dysfunction in homocystinuria patients. In this work we performed a retrospective analysis of fibroblast data from 62 homocystinuria patients and found that 13 of them presented a disturbed SAM activation. Their genotypic background was identified and the corresponding CBS mutant proteins were produced in E. coli. Nine distinct mutations were detected in 22 independent alleles: the novel mutations p.K269del, p.P427L, p.S500L and p.L540Q; and the previously described mutations p.P49L, p.C165Rfs*2, p.I278T, p.R336H and p.D444N. Expression levels and residual enzyme activities, determined in the soluble fraction of E. coli lysates, strongly correlated with the localization of the affected amino acid residue. C-terminal mutations lead to activities in the range of the wild-type CBS and to oligomeric forms migrating faster than tetramers, suggesting an abnormal conformation that might be responsible for the lack of SAM activation. Mutations in the catalytic core were associated with low protein expression levels, decreased enzyme activities and a higher content of high molecular mass forms. Furthermore, the absence of SAM activation found in the patients’ fibroblasts was confirmed for all but one of the characterized recombinant proteins (p.P49L). Our study experimentally supports a deficient regulation of CBS by SAM as a frequently found mechanism in CBS deficiency, which should be considered not only as a valuable diagnostic tool but also as a potential target for the development of new therapeutic approaches in classical homocystinuria. 相似文献