降糖方对糖耐量异常大鼠糖脂代谢及骨骼肌组织IκB-α和NF-κBp65的影响

目的:探讨降糖方对糖耐量异常大鼠糖脂代谢及骨骼肌组织κB抑制蛋白α(IκB-α)、核转录因子肽p65(NF-κBp65)的影响。方法:SD大鼠随机分为正常组、模型组、盐酸二甲双胍组、降糖方低、高剂量组,采用高脂饮食喂养4周,链脲佐菌素(STZ)腹膜内注射建立糖耐量异常大鼠模型,治疗8周后,观察骨骼肌组织病理变化,测定空腹血糖(FPG)、血清胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、骨骼肌组织IκB-α mRNA、NF-κBp65 mRNA水平、骨骼肌组织白细胞介素-4(IL-4)、白细胞介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)蛋白水平。结果:正常组大鼠骨骼肌组织结构正常,模型组骨骼肌排列紊乱、肌纤维断裂、可见明显炎症细胞浸润;盐酸二甲双胍及降糖方干预后,骨骼肌炎症细胞浸润减少,肌纤维排列趋于整齐。模型组大鼠FPG、FINS、HOMA-IR水平、血清TG、TC、LDL-C水平、骨骼肌IκB-α mRNA、NF-κBp65 mRNA和蛋白水平、骨骼肌组织IL-4、IL-12、TNF-α蛋白水平高于对...     

乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制

乙酰基二肽-1鲸蜡酯（acetyl dipeptide-1 cetyl ester,AD-1）是乙酸和十六烷基醇与精氨酸和酪氨酸组成的人工合成肽,具有一定的抗炎和提高皮肤屏障功能的作用,已被用于敏感肌化妆品中。该成分虽然也被用于抗衰老化妆品中,但缺乏公开的系统科学研究。本研究通过评价AD-1的体外抗氧化和抗糖基化作用、建立UVA反复照射真皮原代成纤维细胞的光老化模型,探讨其在光老化细胞中的抗衰老活性。采用MTT法测定AD-1对细胞活力的影响;RT-qPCR法检测AD-1和UVA对衰老相关p21、p53、MMPs、IL6、Col1、Col3以及自噬相关p62、ATG5、ATG7 mRNA表达的影响;Western blot法检测AD-1和UVA对衰老及自噬相关p16、p21、p53、Col1、LC3B、p62蛋白水平的影响;β-半乳糖苷酶染色和MDC染色评价细胞衰老损伤,并通过荧光探针DCFH-DA检测细胞内活性氧水平。实验结果显示,AD-1干预可以减少UVA诱导的细胞损伤和mRNA表达异常,改善p16、p21、p53、Col1、LC3B、p62蛋白水平异常。结果表明,AD-1具有一定的...     

An ethological analysis of the effects of chlordiazepoxide and bretazenil (Ro 16-6028) in the murine elevated plus-maze

The effects of chlordiazepoxide (2.5-15.0mg/kg), a full benzodiazepine receptor agonist, and bretazenil (5.0-30.0mg/kg), a partial benzodiazepine receptor agonist, were examined in the murine elevated plus-maze paradigm. Behaviours recorded comprised the traditional indices of anxiety as well as a number of ethologically derived measures. Results show that chlordiazepoxide (10-15mg/kg) and bretazenil (5-30mg/kg) not only decreased traditional indices of anxiety but also reduced risk assessment behaviours such as head-dipping and stretch attend postures from secure areas of the maze. Both compounds produced these effects without adversely affecting general activity levels. While traditional indices of anxiety did not clearly discriminate between the two compounds, some differences were apparent on the ethological measures. The dose-response curves for bretazenil were generally shallower than those for chlordiazepoxide, confirming its partial agonist profile. Together, these data support the view that benzodiazepine receptor partial agonists may have utility in the management of human anxiety disorders.     

Perceptions of practice problems encountered by family physicians, pediatricians, and orthopedic surgeons

Information about physicians' practice problems was solicited through a structured questionnaire mailed to a group of family physicians, pediatricians, and orthopedic surgeons. Overall, a lack of personal time was the major concern across the three groups of physicians. Comparisons among the three types of physicians revealed two patterns: Family physicians reported more concerns in the "interpersonal" dimension, whereas orthopedic surgeons had more concerns in the "legal-economic" dimension. These patterns of differences persisted with two variables controlled: gender and time period in which they completed their residency program. These findings indicate that physicians' concerns in their practice vary among the specialties, and they imply that the changed economy and reimbursement system might have more impact on one than the other. Thus the effectiveness of residency training and continuing education might be improved by emphasizing the specialty-related problems in practice.     

Autologous peripheral blood stem cell transplantation in the patients with hematologic malignancies and solid tumors

Objective To evaluate the long-term therapeutic effects of autologous peripheral blood st em cell transplantation (auto-PBSCT) on the treatment of hematological and soli d tumors. Methods Fifty-one patients were recruited in this auto-PBSCT study, in which several p otentially important parameters were studied including the optimal time for stem cell co llection, the dose of stem cell reinfusion, the time of hematopoietic reconsti tution, the disease free survival (DFS) and overall survival (OS), complication s related to transplantation, and maintenance chemotherapy after auto-PBSCT. Results After APBSCT, 3-year and 5-year survival rates of NHL were 83.3%; those of AM L were 74.7%; those of MM were 37.9% and 19%; those of ALL were 40% and 0% res pectively. Hematopoietic reconstitution was greatly promoted by granulocyte col ony stimulating factor (G-CSF). The mean time for patients’ neutrophil to reco ver up to >0.5×10(9) /L after APBSCT was 11.14 days in the group of the patien ts receiving G-CSF in contrast to 17.6 days in the group receiving no G-CSF. The most common complications of transplantation were fever, liver dysfunction and hypokalaemia, which were curable. No death was due to transplantation related complications.Conclusion Comparing with conventional chemotherapy, our study suggests that auto-PBSCT i s a very important therapeutic option that can significantly improve the prognos is in the patients with hematological and solid tumors, especially in the patien ts with AML and NHL.     

Effects of benactyzine on action potentials and contractile force of guinea pig papillary muscles

ＩＮＴＲＯＤＵＣＴＩＯＮＢｅｎａｃｔｙｚｉｎｅｉｓａｓｙｎｔｈｅｔｉｃｂｌｏｃｋｅｒｏｆＭ ｒｅｃｅｐｔｏｒｐｏｓ ｓｅｓｓｉｎｇｓｍｏｏｔｈｍｕｓｃｌｅｒｅｌａｘａｎｔｐｒｏｐｅｒｔｉｅｓａｎｄｑｕｉｎｉｄｉｎｅ ｌｉｋｅａｃｔｉｏｎｏｎｈｅａｒｔ[1] .Ｂｅｎａｃｔｙｚｉｎｅｉｓｅｆｆｅｃｔｉｖｅｉｎｒｅｓｔｏｒ ｉｎｇｃｉｒｃｕｌａｔｏｒｙｆｕｎｃｔｉｏｎｄｉｓｔｕｒｂｅｄｂｙｏｒｇａｎｏｐｈｏｓｐｈａｔｅｐｏｉ ｓｏｎｉｎｇ[2 ,3] .Ｐｒｅｖｉｏｕｓｓｔｕｄｉｅｓｓｕｇｇｅｓｔｅｄｔｈａｔｂｅｎａ…     

THAP11通过抑制p53的泛素化影响食管癌细胞的增殖和凋亡

目的：探讨死亡相关蛋白(thanatos-associated protein,THAP)11对食管癌细胞增殖和凋亡的影响及其潜在 的机制。方法：采用蛋白质印迹法检测人食管上皮细胞Het-1A和人食管癌细胞(Eca109,TE-1和Ec 9706)中THAP11的 表达。将食管癌TE-1细胞分为空白对照(NC)组、阴性对照(LV-LC)组、TRA P11(LV-TRA P11)组,按分组处理细胞后, 采用MTT 法检测细胞活力,流式细胞术检测细胞凋亡,caspases试剂盒检测caspase-3和caspase-9的活性。采用体外泛素 化实验检测TE-1细胞的p53泛素化水平。结果：与Het-1A细胞相比,食管癌细胞中THAP11的表达显著下降(P<0.05)。 LV-THAP11转染食管癌细胞后,细胞活力降低(P<0.05),凋亡率升高(P<0.05),caspase-3和caspase-9活性升高(P<0.05)。 THAP11能够提高食管癌细胞中p53蛋白的表达(P<0.05)。与LV-LC组相比,转染THAP11后食管癌细胞中p53的表达上调 (P<0.05),MDM2调节的p53的泛素化也被抑制。结论：THAP11通过抑制p53的泛素化抑制食管癌细胞的增殖,促进食管 癌细胞的凋亡。