龙岩市启用国家免疫监测信息管理系统后常规免疫报告质量分析

目的 评价龙岩市2004年启用“国家免疫监测信息管理系统”后常规接种率监测报告质量.方法 用差值评价法、比值评价法分析2004年度各县 （市、区）上报的常规免疫监测数据.结果 龙岩市2004年县、乡两级均能及时完成1年6次的监测报告工作,五苗报告接种率均在90%以上;估计接种率与报告接种率有一定偏差,7个县（市、区）5种疫苗判定为“可信”的只有5个;通过R值评价发现,报告的麻疹疫苗应种数明显偏少.结论 龙岩市2004年度常规免疫监测系统运转良好;用现行的运转模式很难提高常规免疫监测数据的准确性,需要推广使用儿童计划免疫智能卡信息管理系统.     

危重病患者血清细胞因子水平的变化及纳洛酮的影响

目的探讨危重病患者血清细胞因子IL-6、TNF—α的变化及纳洛酮对IL-6、TNF—α含量的影响。方法分两步：①138例危重病患者按病因分成4组；感染组（n=32）、创伤组（n=30）、中风组（n=30）、休克组（11=46），与正常对照组（n=20）进行比较，用放免法测定其IL-6、TNF—α急性期变化。②随后对休克组病人随机分为两个组：常规治疗组（A组）及常规治疗＋纳洛酮治疗组（B组），测定两组治疗前后不同时间点IL-6、TNF—α的含量变化。结果①IL-6、TNF—α含量在感染组、创伤组、中风组及休克组均显著升高，较正常对照组差异显著（P〈0．01）。各组中又以休克组最为明显。②B组纳洛酮治疗后不同时间点IL-6、TNF—α水平明显低于A组，而且TNF—α水平下降速度快于IL-6。结论①IL-6、TNF—α参与了SIRS向MODS的发生发展，可反映病情严重程度。②早期应用纳洛酮可降低IL-6、TNF—α的水平，可能是治疗休克发挥临床作用的一个新的重要机制。     

针刺麻醉及针药复合麻醉在腹部外科的应用

针刺麻醉（acupuncture anesthesia，AA，以下简称针麻）下手术创始于20世纪50年代末，经历了初创期（1959年～1966年），发展期（1967年～1979年），巩固期（1980年至今）3个阶段。现将针麻及针药复合麻醉在腹部外科的应用总结如下。1针麻及针药复合麻醉的优越性1．1针麻的基本作用：①术中镇痛；②抗术中组织牵拉反应；③抗创伤性休克；④抗感染；⑤促进术后创伤组织恢复。简言之，针麻有利于患者生命体征、内环境的稳定，从而保证手术顺利进行、加速患者术后恢复。但对于健康机体的效果则不显著。     

HPLC法测定盐酸左氧氟沙星血浓度

目的:建立HPLC法测定血清中盐酸左氧氟沙星浓度。方法:采用三氯乙酸提取血样,以盐酸洛美沙星为内标,色谱柱:shim-pack C_(18)柱(250 mm×4．6 mm,5μm),流动相为乙腈:50 mmol·L~(-1)柠檬酸:1 mol·L~(-1)醋酸胺(16:83:1),流速1．0 ml·min~(-1),检测波长295 nm,灵敏度0．01AUFS。结果:本法在0．1～10μg·ml~(-1)之间线性良好(r=0．999 9),日间和日内RSD小于10％,最低检测浓度为0．1μg·ml~(-1)。结论:本法快速、准确、重现性好,可为临床血药浓度监测和药物动力学提供依据。     

湘产“湘蕾”系列灰毡毛忍冬HPLC指纹图谱研究

目的建立湘产“湘蕾”品种灰毡毛忍冬药材的指纹图谱，为“湘蕾”品种灰毡毛忍冬药材质量的评价提供科学依据。方法高效液相色谱法，运用“中药色谱指纹图谱相似度评价系统2004A版”进行数据处理，构建灰毡毛忍冬对照指纹图谱。结果不同灰毡毛忍冬药材指纹图谱相似度较好，确定了10个共有峰，建立了“湘蕾”品种灰毡毛忍冬HPLC指纹图谱。结论该方法准确、稳定、可靠，可用于“湘蕾”品种灰毡毛忍冬的质量控制。     

CC chemokine ligand 25 enhances resistance to apoptosis in CD4+ T cells from patients with T-cell lineage acute and chronic lymphocytic leukemia by means of livin activation

We investigated CD4 and CD8 double-positive thymocytes, CD4(+) T cells from typical patients with T-cell lineage acute lymphocytic leukemia (T-ALL) and T cell lineage chronic lymphocytic leukemia (T-CLL), and MOLT4 T cells in terms of CC chemokine ligand 25 (CCL25) functions of induction of resistance to tumor necrosis factor alpha (TNF-alpha)-mediated apoptosis. We found that CCL25 selectively enhanced resistance to TNF-alpha-mediated apoptosis in T-ALL and T-CLL CD4(+) T cells as well as in MOLT4 T cells, but CD4 and CD8 double-positive thymocytes did not. One member protein of the inhibitor of apoptosis protein (IAP) family, Livin, was selectively expressed in the malignant cells at higher levels, particularly in T-ALL CD4(+) T cells, in comparison with the expression in CD4 and CD8 double-positive thymocytes. After stimulation with CCL25 and apoptotic induction with TNF-alpha, the expression levels of Livin in these malignant cells were significantly increased. CCL25/thymus-expressed chemokine (TECK), by means of CC chemokine receptor 9 (CCR9) ligation, selectively activated Livin to enhance resistance to TNF-alpha-mediated apoptosis in c-jun-NH(2)-kinase 1 (JNK1) kinase-dependent manner. These findings suggested differential functions of CCR9/CCL25 in distinct types of cells. CD4 and CD8 double-positive thymocytes used CCR9/CCL25 for migration, homing, development, maturation, selection, cell homeostasis, whereas malignant cells, particularly T-ALL CD4(+) T cells, used CCR9/CCL25 for infiltration, resistance to apoptosis, and inappropriate proliferation.     

脐动脉血流速度检测及胎心监护对子宫内缺氧的预测价值

目的：比较多普勒超声脐动脉血流速度检测和胎儿监护－无负荷试验（NST）对胎儿宫内缺氧的预测价值。方法：检测4326例正常妊娠妇女的脐动脉收缩期最大血流速度（S）与舒张末期血流速度（D）的比值（S／D）、脐动脉血流博动指数（PI）、脐动脉血流阻力指数（RI）、快速血流速比（FVR）,建立妊娠晚期各孕周相应的正常值。同时对2873例正常晚期妊娠妇女,进行多普勒超声脐动脉血流速度检测和NST,追踪检测后1周内出生的新生儿情况,并与检测结果对照。结果：（1）正常妊娠妇女S／D、PI、RI值随孕周的增加而逐渐下降,FVR值则变化不大;（2）脐动脉血流S／D、PI、RI、FVR值和NST分别异常者,新生儿出生时表现有宫内缺氧情况者分别为62．0％、60．7％、63．3％、27．0％和75．7％,而脐动脉血流S／D、PI、RI、FVR值和NST正常者,新生儿出生时表现有宫内缺氧情况者分别为12．8％、13．9％、13．0％、15．7％和7．5％,两者比较差异有极显著性（P＜0．01）。（3）多因素回归分析显示,RI和NST两个变量与胎儿宫内缺氧关系密切,差异有显著性（P＜0．05）。结论：NST和RI值在正常妊娠妇女中,预测宫内缺氧的临床价值较好,多指标检测分析比单一指标更准确。     

Ultrasound and Microbubbles： Their Functions in Gene Transfer In Vitro

To examine the role of ultrasound in gene delivery in vitro, three cells lines were exposed to the low-frequency ultrasound of varying intensities and for different durations to evaluate their effect on gene transfection and cell viability of the cells. Microbubble （MB）, Optison （10%）, was also used to observe the role of the microbubbles in gene transfection. The results demonstrated that as the ultrasound intensity and the exposure time increased, the gene transfer rate increased and the cell viability decreased, but at high energy intensities, the cell viability decreased dramatically, which caused the transfer rate to decrease. The most efficient ultrasound intensity for inducing gene transfer was 1 W/cm^2 with duration being 20 s. At the same energy intensity, higher ultrasound intensity could achieve maximal gene transfer rate earlier. Microbubbles could increase ultrasound-induced cell gene transfer rate by about 2 to 3 times mainly at lower energy intensities. Moreover, microbubbles could raise the maximum gene transfer rate mediated by ultrasound. It is concluded that the low-frequency ultrasound can induce cell gene transfer and the cell gene transfer rate and viability are correlated with not only the ultrasound energy intensity but also the ultrasound intensity, the higher ultrasound intensity achieves its maximal transfer rate more quickly and the ultrasound intensity that can induce optimal gene transfer is 1 W/cm^2 with duration being 20 s, and microbubbles can significantly increase the maximal gene transfer rate in vitro.     

肝瘀热互结与肝纤维化关系的临床研究

探讨肝瘀热互结与肝纤维化之间的关系,为临床清热化瘀抗肝纤维化提供合理用药依据,选择肝病瘀热互结与非瘀热互结患者各２０例,分别给予清热化瘀方和辨证方药治疗,并于治疗前后分别以放射免疫法（ＲＩＡ）测定血清Ⅳ型胶原（ＣⅣ）和透明质酸（ＨＡ）,以生化比色法测定脯氨酸肽酶（ＰＬＤ）,并作相关分析,结果表明肝瘀热至结患者血清ＣⅣ,ＨＡ水平明显高于非瘀热互结患者;瘀热互结程度与肝纤维化指标水平呈正相关;清热化瘀