幼儿外侧膝状体微血管的形态学分析

目的分析幼儿外侧膝状体微血管的形态结构。方法采用8例死亡24 h内的幼儿外侧膝状体,经碱性磷酸酶染色、光镜观察及体视学处理,对幼儿外侧膝状体微血管密度进行分析。结果幼儿外侧膝状体后2/3部微血管密度高于前1/3部的微血管密度,经t检验,P<0.01。结论①幼儿阶段外侧膝状体与其相对应的视皮质各区微血管密度的大小相一致。②按照功能与结构相一致的原则,本课题研究结果支持“直接兴奋性输入会聚学说”。     

青少年抑郁症患者性激素受体基因微卫星多态性与功能失调性认知的关系

目的探讨青春期首发的青少年抑郁症患者性激素受体基因微卫星多态性是否与功能失调性认知存在联系。方法采用聚合酶链式反应（PcR）结合毛细管电泳和基因扫描技术。检测青春期首发的青少年抑郁症患者雄激素受体（AR）、雌激素受体α（ERα）、雌激素受体β（ERβ）基因重复序列多态性的重复片段长度。应用《功能失调性态度问卷》（DAS）对青少年抑郁症患者进行评估，并把评估结果和微卫星多态片段长度进行相关性和对照性分析。结果1．女性患者ERβ基因微卫星多态性与DAS总分呈显著仉相关；2．对各位点等位基因分长短片段作组间比较。发现ERα短片段组DAS总分显著高于长片段组。男性患者不存在这种差异性。3．AR受体与功能失调性认知不存在这种联系。结论女性青少年ERα、ERβ受体基因微卫星多态可能与功能失调性认知存在联系，而男性青年抑郁症患者可能不存在这种联系。     

Outcomes of using Firebird rapamycin eluting stents in routine coronary intervention practice: one-year results from the pilot study of Firebird in China registry

Recent clinical trials with rapamycin-eluting stents have shown very low restenosis rates.1-4 However, the higher penetration of drug eluting stent (DES) in China is being limited by the high costs of these imported devices, especially when considering multiple stenting.     

急性颈脊髓损伤并呼吸功能障碍高危因素分析

目的分析急性颈脊髓损伤后并发呼吸功能障碍的高危因素，对高危病人采取更积极治疗措施，以减少呼吸功能障碍发生，降低死亡率。方法对48例急性颈脊髓损伤病例进行分析，了解瘫痪程度、脊髓损伤平面、是否吸烟及年龄与呼吸功能障碍发生率的相关性，规定3周内为急性期。结果22例发生呼吸功能障碍，9例死亡，7例死于呼吸衰竭，1例心跳骤停死亡，1例合并脑干损伤死亡。结论全瘫、脊髓损伤平面高，吸烟、高龄是急性颈脊髓损伤并发呼吸功能障碍的高危因素，对高危病人气管切歼、呼吸机辅助呼吸态度应积极。     

Influence of the femoral head size on early postoperative gait restoration after total hip arthroplasty

Background We investigated the effects of using large-diameter femoral heads in total hip prostheses on eady postoperative gait restoration in patients undergoing total hip arthroplasty (THA). Methods We collected data for 19 primary THAs using 28-mm metal-on-polyethylene heads (conventional group) and for 12 THAs (BHR group) using metal-on-metal femoral heads with an average size of 45 mm (range, 40-49 mm). All patients had unilateral femoral head avascular necrosis. All patients underwent Harris Hip Scores evaluation and gait analysis with the IDEEA device at the same 3 time points which were before surgery and then at 1 month and again at 3 months after surgery, and the parameters measured were walking speed, stride length (SL), single limb support (SLS), cycle duration (CD), and swing power (SP). Harris Hip Scores and gait analysis parameters for both groups were compared.Results Intraclass comparison indicated that Harris Hip Scores, speed scores, and gait parameter measures in both groups improved significantly with the passage of time; Interclass comparison showed no significance between Harris1m postop - Harrispreop and Harris3m postop- Harrispreop in both groups. The speed in the BHR group at 1 month and at 3 months after surgery was significantly higher than that of conventional group. At 1 month after surgery, each mean for SLnormal-SLaffected, (SLSnormal - SLSaffected)/CD, and SPnormal - SPaffocted in the BHR group was significantly lower than that for the conventional group. At 3 months after surgery, the differences between means for both groups for SLnormal - SLaffected.(SLSnormal- SLSaffected)/CD, and SPnormal - SPaffected were not significant, but the mean of SPnormal - SPaffected in the BHR group was significantly lower than that in the conventional group.Conclusions Our data suggest that large-diameter femoral heads in THA provide better early gait restoration than conventional-size femoral heads.     

重亚硫酸盐修饰直接测序技术检测p16基因甲基化的研究

目的建立稳定的重亚硫酸盐直接测序技术，检测p16基因甲基化状况。方法提取正常人全血基因组DNA．建立起稳定的重亚硫酸盐直接测序平台，利用该技术检测结直肠癌细胞株pl6基因甲基化状况。结果应用列联表的Fisher，Exact Test比较3种纯化方法的测序结果，乙醇／醋酸钠和过柱纯化与SAP—ExonI纯化法比较，测序结果差异有统计学意义（P〈0．05）；应用重亚硫酸盐直接测序技术，可检测出p16基因目的片段中CpG岛所有CpG位点的甲基化状况。结论应用SAP—ExonI纯化法，建立了稳定的重亚硫酸盐直接测序技术，并可检测出目的片段中CpG岛所有CpG位点的甲基化状况。     

清热抗感冲剂对小鼠IL-2水平影响的实验研究

目的:通过检测清热抗感冲剂对实验性免疫低下小鼠及正常小鼠血清IL-2水平的影响,探讨该冲剂对免疫功能的调节作用.方法:选取昆明种小鼠100只,雌雄各半,其中50只腹腔注射环磷酰胺3mg/0.2mL/只,每天给药1次,连续7天,造成免疫低下小鼠,然后随机分为模型组、中药对照组(银翘解毒片组)、西药对照组(γ-干扰素组)、小剂量组、大剂量组;其余50只随机分为正常空白对照组(即生理盐水组)、中药对照组、西药对照组、小剂量组、大剂量组.上述各组连续用药3天后,处死,采血,离心取血清,采用放免γ测量仪检测IL-2含量.结果:清热抗感冲剂能显著提高正常小鼠和注射环磷酰胺引起的免疫低下小鼠血清中IL-2生成水平(统计学处理P＜0.01).结论:清热抗感冲剂对小鼠的细胞免疫功能有明显的增强作用.     

Pivotal study of iodine-131-labeled chimeric tumor necrosis treatment radioimmunotherapy in patients with advanced lung cancer.

PURPOSE: Tumor necrosis treatment (TNT) uses degenerating tumor cells and necrotic regions of tumors as targets for radioimmunotherapy. Previous studies in animal tumor models and clinical trials have demonstrated that when linked to the therapeutic radionuclide iodine-131, recombinant chimeric TNT antibody ((131)I-chTNT) can deliver therapeutic doses to tumors regardless of the location or type of malignancy. Therapeutic efficacy and toxicity of (131)I-chTNT in advanced lung cancer patients were studied in this pivotal registration trial. PATIENTS AND METHODS: Patients with advanced lung cancer were treated with systemic or intratumoral injection of (131)I-chTNT in eight oncology centers in China. The objective response rate (ORR) was assessed as the primary end point. RESULTS: All 107 patients who were entered onto the study and completed therapy had experienced treatment failure after prior radiotherapy or chemotherapy a mean of three times. The results showed an ORR of 34.6% (complete response, 3.7%; partial response, 30.8%; no change, 55.1%; and progressive disease, 10.3%) in all patients and 33% in 97 non-small-cell lung cancer patients. A biodistribution study demonstrated excellent localization of the radioactivity in tumors in both systemically and intratumorally injected patients. The most obvious adverse side effect was mild and reversible bone marrow suppression. CONCLUSION: Radioimmunotherapy with (131)I-chTNT was well tolerated and can be used systemically or locally to treat refractory tumors of the lung.     

糖皮质类固醇激素治疗急性肺损伤的进展

急性肺损伤(ALI)是全身炎症反应综合征在肺部的表现,炎症失控是其病变本质。糖皮质类固醇激素因其抗炎和免疫抑制特性而被广泛应用于临床重症疾病。本文在复习近年来文献的基础上就糖皮质激素在ALI中的应用进展作一综述。     

Comparing the efficacy of different intra-articular injections for knee osteoarthritis: A network analysis

Background:The findings on the effectiveness of platelet-rich plasma, ozone, and hyaluronic acid in the treatment of osteoarthritis of the knee are controversial, and the existing original studies and meta-analyses are mostly comparisons of a single joint cavity injection method, lacking direct and indirect comparisons of different drugs in the joint cavity. The lack of direct and indirect comparisons of different drugs in the joint cavity makes it difficult to have a clearer and more comprehensive understanding of joint cavity injection methods. In this study, the efficacy of platelet-rich, ozone, sodium hyaluronate, and combined knee cavity injections were compared directly or indirectly using a reticulated meta-analysis in this field, and the efficacy of treatment measures was ranked to provide more comprehensive and reliable evidence-based clinical evidence for the selection of knee cavity injections in osteoarthritis of the knee.Objective:To compare the effects of platelet-rich plasma, ozone, and sodium glassate injection interventions on the efficacy of osteoarthritis of the knee through reticulated Meta-analysis, and to comprehensively compare the clinical effectiveness of platelet-rich plasma, ozone, and sodium glassate injection joint cavity injection for the treatment of osteoarthritis of the knee.Methods:The PubMed, CBM, CNKI, VIP, and Wan-Fang databases were searched for information on the effectiveness of platelet-rich plasma, ozone, and sodium vitrate injection for the comparative treatment of osteoarthritis of the knee, with a search time frame of each database from the date of creation to July 20, 2021. Two investigators independently screened the literature, extracted data according to inclusion and exclusion criteria, and evaluated the quality of the literature in parallel. Statistical analysis was performed using Stata 16.0 software to compare the differences in the efficacy of each treatment measure using the ratio and 95% confidence interval as effect indicators and to rank the efficacy.Results:Thirty-three RCTs with 7003 patients with osteoarthritis of the knee were included, involving 5 therapeutic measures. Meta-analysis showed that the efficacy of platelet-rich plasma injection was superior to both ozone and hyaluronic acid therapies. Hyaluronic acid+ozone and platelet-rich plasma+hyaluronic acid were both superior to ozone and hyaluronic acid monotherapy. The differences in efficacy between hyaluronic acid and ozone compared with platelet-rich plasma were statistically significant, and the differences in efficacy between the 2 combination therapies (platelet-rich plasma+hyaluronic acid, hyaluronic acid+ozone) and the 3 monotherapies (platelet-rich plasma, ozone, hyaluronic acid) were statistically significant. Platelet-rich plasma+hyaluronic acid, hyaluronic acid+ozone compared with 3 monotherapies (platelet-rich plasma, ozone, hyaluronic acid) were statistically significant, except for the difference in efficacy with platelet-rich plasma, which was not statistically significant, indicating that this platelet-rich plasma+hyaluronic acid and Hyaluronic acid+ozone combination therapy was superior to monotherapy. Also, the efficacy of platelet-rich plasma was better than hyaluronic acid and ozone and the difference was statistically significant, indicating that platelet-rich plasma was more effective than ozone and sodium glass in the treatment of osteoarthritis of the knee in monotherapy.Conclusion:It is believed that in the course of clinical practice, hyaluronic acid+ozone or platelet-rich plasma+hyaluronic acid combination therapy or platelet-rich plasma therapy can be preferred for patients with osteoarthritis of the knee.