感音神经性耳聋患者大前庭导水管综合征相关SLC26A4基因IVS7-2A＞G的全序列分析

目的 对携带SLC26A4基因IVS7-2A＞G单杂合突变感音神经性耳聋患者进行 SLC26A4基因的全序列检测,以期发现除IVS7-2A＞G以外的其他突变.方法 应用直接测序法对80例携带IVS7-2A＞G单杂合突变的感音神经性耳聋患者进行SLC26A4基因进行全序列测序.结果 80例患者中47例发现另1个突变位点,其余33例未发现复合杂合突变,IVS7-2A＞G单杂合突变找到另外1个突变的比例为58.8%(47/80).发现了 3个新的突变,分别是5+2T＞A、14-2A＞G和1825del G,最为常见的5种突变为H723R(20%)、T410M(5%)、15+5G＞A(5%)、L676Q(5%)、N392Y(3.75%).第17外显子是突变发生种类最多的外显子.结论 SLC26A4基因IVS7-2A＞G单杂合突变者应该进行其他突变的筛查,SLC26A4基因复合突变可以解释部分的耳聋原因.     

Cardiac vagal control predicts spontaneous regulation of negative emotional expression and subsequent cognitive performance

The present research investigated whether cardiac vagal control (as measured by respiratory sinus arrhythmia, RSA) predicts an individual's predisposition to suppress negative emotional expressions. One hundred thirty-six participants watched either a negative film or a neutral film. Facial expressions were recorded during the film and subjective emotional responses were assessed afterwards. Participants performed verbal and spatial working memory tasks both before and after the film clips. We found that resting RSA modulated the degree of coherence between facial expressions of emotion and subjective emotional experience in the negative film condition. Specifically, participants with higher resting RSA expressed less but reported feeling just as much negative emotion as those with lower resting RSA. Moreover, higher resting RSA predicted smaller pre-film to post-film improvements in spatial working memory performance in the negative film condition, suggesting that expressive suppression among high RSA participants temporarily undermined the operation of working memory. In the neutral film condition, resting RSA did not relate to expressive or subjective responses or subsequent working memory performance. These results support the notion that cardiac vagal control reflects an internal marker of self-regulatory tendencies and suggest that spontaneous self-regulation associated with individual differences in resting RSA may temporarily deplete self-regulatory resources.     

Diversity of nitrogenase (nifH) genes pool in soybean field soil after continuous and rotational cropping

Diazotrophs diversity in soybean is a topic requiring thorough investigation since the previous researches have focused on only rice, forest, grass, water, etc. In this research, iron‐only nitrogenase nifH gene was as genetic marker. PCR‐RFLP was used to investigate the difference of diazotrophs community diversity in the soil from the continuous cropping (CC) (the 5‐yr tilling of soybean) and the rotational cropping (RC) (soybean‐corn) soils in the northeast of China. A total of 36 isolates were genetically characterized. Most of the isolates closely related to Azospirillum and Azotobacter. Eighty‐six unique nifH gene sequences were obtained by cloning of the respective PCR products in two soil samples. It was found that the diversity of nifH genes in CC changed obviously compared with RC. Phylogenetic analysis indicated that most of the clones clustered together in a high homogeneity with some sequence retrieved from environmental representatives. The sequence diversity of nifH genes was high and the members of the Alphaproteobacteria were predominant in both samples. The experimental study also revealed the two non‐proteobacterial diazotrophs, firmicutes and euryarchaeota. Through this study, it can be assumed that different tillage perhaps affected the nifH gene‐containing population diversity. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

切应力作用下肾近端小管上皮细胞纤溶酶原激活物(tPA和uPA)表达的变化及意义

检测切应力作用下肾近端小管上皮细胞纤溶酶原激活物tPA和uPA mRNA表达的变化,探讨糖尿病肾病早期小管间质细胞外基质重塑的可能机制.用5 dyn/cm2和10 dyn/cm2的切应力处理肾近端小管上皮细胞(NRK-52E),作用时间分别为1、3和6 h,用RT-PCR法检测tPA及uPA mRNA的表达.结果表明:切应力呈大小和时间依赖性下调肾小管上皮细胞tPA及uPA mRNA的表达.在糖尿病肾病早期,高滤过引起的切应力增加可抑制肾近端小管上皮细胞tPA和uPA mRNA表达,导致肾小管间质纤维蛋白溶解活性降低,参与小管间质细胞外基质的重塑.     

Antigenic and genomic relation between human influenza viruses that circulated in Argentina in the period 1995-1999 and the corresponding vaccine components.

BACKGROUND: The analysis of epidemic influenza virus has been focused on antigenic and genomic characterization of the hemagglutinin (HA) glycoprotein in order to detect new variants for the recommendation of the vaccine strains in each season. Since October 1998, WHO organized a second meeting to evaluate the vaccine formula for the southern hemisphere. OBJECTIVES: (a) Present the antigenic and genomic characterization of influenza strains obtained from the Argentina surveillance network, (b) compare between strains collected in Argentina and other countries with the vaccine formula strains used in each season. STUDY DESIGN: Influenza strains were collected during a 5-year period (1995-1999). Initially, laboratory diagnosis was done by immunofluorescence (IF) assay on clinical samples, followed by viral isolation in Madin-Darby canine kidney (MDCK) cells. The isolates were characterized antigenically by hemagglutination-inhibition (HI) assay with post-infection ferret antisera. The genomic characterization consisted on RT-PCR followed by sequencing of the HA1 portion of the HA gene. The comparison between reference and circulating strains was analyzed by the construction of phylogenetic trees. RESULTS: The H3N2 circulating strains matched the corresponding vaccine component only in 1999, the first year when a vaccine recommended specifically for the southern hemisphere was used. Besides, H1N1 circulating strains matched the corresponding vaccine component only in 1998. Regarding to influenza B, only in 1995, the circulating strains showed no match to the B vaccine component. CONCLUSIONS: The results showed the usefulness of an intensified influenza laboratory surveillance to access the correct vaccine and the importance of having the necessary tools to characterize the circulating strains.     

低氧诱导因子1α对子宫颈癌细胞生物学行为的影响

目的探讨低氧诱导因子1α（HIF-1α）对宫颈癌细胞的生物学行为的影响以及其中可能存在的分子机制。方法通过CoCl2化学诱导宫颈癌HeLa细胞缺氧;构建靶向HIF-1α的反义真核表达载体、经脂质体介导转染HeLa细胞的方法沉默HIF-1α的表达。将实验细胞分为常氧未转染对照（NN）组、常氧空质粒转染对照（NI）组、常氧转染pcDNA3．0／HIF-1α质粒（NT）组、缺氧未转染对照（HN）组、缺氧空质粒转染对照（HI）组、缺氧转染pcDNA3．0／HIF-1α质粒（HT）组。用四甲基偶氮唑蓝法、Transwell侵袭小室方法观察各组细胞的增殖、侵袭能力的改变,用流式细胞仪检测各组细胞的凋亡率,用RT-PCR技术检测各组细胞目的基因HIF-1α及其靶基因血管内皮生长因子（VEGF）、葡萄糖转运体1（GLUT1）、多药耐药基因1（MDR1）的表达变化。结果NT组细胞在培养12、24、48、72h时的活细胞数分别为0．053±O．003、0．074±0．004、0．148±0．015、0．192±0．038,而HT组分别为0．069±0．003、0．155±0．022、0．224±0．022、0．308±0．069;NT和HT组的细胞增殖受到抑制,NT组与NN及NI组、HT组与HN及HI组间的活细胞数分别比较,差异均有统计学意义（P〈0．01）。各组细胞的凋亡率分别是,NN组（29．27±0．18）％、NI组（31．13±0．08）％、NT组（51．11±0．14）％、HN组（11．46±0．28）％、HI组（15．77±0．49）％、HT组（40．05±0．97）％;HT组与HN及HI组、NT组与NN及NI组间分别比较,差异均有统计学意义（P〈0．01）。各组细胞的侵袭能力由高到低依次为HI、HN、NI、NN、HT、NT组,分别为（40±9）％、（37±12）％、（28±5）％、（26±7）％、（19±7）％、（10±5）％;NT组与NN及NI组、HT组与HN及HI组间分别比较,差异均有统计学意义（P〈0．05）。NT组细胞HIF-1α、VEGF、GLUT1、MDR1 mRNA的相对表达量分别为0．05±0．12、0．09±0．11、0．08±0．15、0．05±0．15,而HT组分别为0．04±0．16、0．16±0．16、0．12±0．20、0．20±0．21;NT组与NN及NI组、HT组与HN及HI组间分别比较,HIF-1α、VEGF、GLUT1、MDR1 mRNA的相对表达量均有降低,差异均有统计学意义（P〈0．01）。结论HIF-1α可能主要通过其下游的靶基因对宫颈癌细胞的恶性生物学行为产生影响,包括抗凋亡、促增殖、增加血液供应和能量供应、耐药等,且体外抑制HIF-1α的表达对宫颈癌细胞有抑制作用。     

血清糖化血红蛋白及脂蛋白-(a)水平变化与糖尿病并发冠心病的关系

目的:探讨血清糖化血红蛋白(HbAlc)及脂蛋白-a[Lp(a)]水平变化与糖尿病并发冠心病的关系。方法:单纯2型糖尿病患者54例(糖尿病组)和2型糖尿病并发冠心病患者128例(糖尿病并发冠心病组),测定各组血清HbAlc和Lp(a)水平,通过Logistic回归和ROC曲线分析血清HbAlc和Lp(a)水平变化对糖尿病并发冠心病的相对危险性预测价值。结果:糖尿病合并冠心病组HbAlc(7.5±1.4)%和Lp(a)(0.25±0.19)g/L明显高于单纯糖尿病组[(6.9±1.4)%和(0.19±0.16)g/L,均P<0.05]。Logistic回归分析显示,血清高水平HbAlc和Lp(a)分别是糖尿病并发冠心病的独立危险因素(OR值2.28,95%CI1.12～4.63,P<0.05)及(OR值2.3,95%CI1.11～4.79,P<0.05)。Logistic回归模型的ROC曲线下面积为0.73(P<0.01)。最佳切点0.7对判断糖尿病患者并发冠心病的敏感性和特异性分别为70%和65%。结论:血清HbAlc和Lp-(a)水平是糖尿病患者并发冠心病的独立危险因素,对评估糖尿病并发冠心病的相对危险性具有临床价值。     

建立听课制度、提高教学质量

为了提高教学质量,学院从课堂教学入手,建立了听课制度,定期开展听课评比活动,有效促进了教师对课堂教学的重视,提高了教学质量。     

Effects of Xinjining(心悸宁) Extract on Inward Rectifier Potassium Current in Ventricular Myocytes of Guinea Pig

<正>Objective:To study the effect of Xinjining extract(心悸宁,XJN) on inward rectifier potassium current(I_(K1)) in ventricular myocyte(VMC) of guinea pigs and its anti-arrhythmic mechanism on ion channel level. Methods:Single VMC was enzymatically isolated by zymolisis,and whole-cell patch clamp recording technique was used to record the I_(K1) in VMC irrigated with XJN of different concentrations(1.25,2.50,5.00 g/L;six samples for each).The stable current and conductance of the inward component of I_(K1) as well as the outward component of peak I_(K1) and conductance of it accordingly was recorded when the test voltage was set on -110 mV.Results:The suppressive rate of XJN on the inward component of I_(K1) was 9.54%±5.81%,34.82%±15.03%,and 59.52%±25.58%with a concentration of 1.25,2.50,and 5.00 g/L,respectively,and that for the outward component of peak I_(K1) was 23.94%±7.45%,52.98%±19.62%,and 71.42%±23.01%,respectively(all P0.05).Moreover, different concentrations of XJN also showed effects for reducing I_(K1) conductance.Conclusion:XJN has inhibitory effect on I_(K1) in guinea pig's VMC,and that of the same concentration shows stronger inhibition on outward component than on inward component,which may be one of the mechanisms of its anti-arrhythmic effect.