Methyl-beta-cyclodextrin treatment affects the thermotropic behaviour of membranes and detergent-resistant membrane fractions of cultured A431 cells

Membranes and detergent-resistant membrane fractions isolated from human epidermoid carcinoma A431 cells after treatment with methyl-beta-cyclodextrin, a compound commonly used in pharmaceutical applications and in manipulation of membrane cholesterol content, display thermotropic transitions at about 15 degrees C and above 37 degrees C, respectively, when analyzed by differential scanning calorimetry. The transitions, absent in untreated cells, were reversible upon cycling through heating and cooling scans, and attributable to lipid components of the membranes, possibly sphingolipids. These results suggest that, after treatment with methyl-beta-cyclodextrin, membranes may show thermotropic transitions, an unusual feature for cellular bilayers, which is likely to influence biological functions.     

Pneumothorax preceding pulmonary blastoma in a child

Serial plain radiographic, ultrasound and CT findings of an unusual case of pulmonary blastoma are described with a review of the literature.     

Biofeedback training to obtain continence in permanent colostomy

Biofeedback training is proposed as rehabilitative training for patients with permanent colostomoies to help them achieve fecal continence. The results of a preliminary study of 18 patients are reported.     

Evaluation of accuracy of an electromagnetic computer-assisted navigation system in total knee arthroplasty

The objective of this study was to develop a method to assess the accuracy of an electromagnetic technology image-free navigation system for total knee arthroplasty in a leg with normal or abnormal mechanical alignment. An acrylic phantom leg was constructed to simulate tibia and femur deformation. Determination of actual leg alignment was achieved using a digital caliper unit. In the setting of normal alignment, the mean error of the system characterised as the difference between the measured computer navigation and digital caliper angles ranged between 0.8 degrees (frontal plane) and 1.5 degrees (lateral plane). In the setting of abnormal alignment, the mean error ranged between 0.4 degrees (frontal plane) and 1.6 degrees (lateral plane). Deformity had no demonstrable effect on accuracy. The study demonstrates satisfactory in vitro system accuracy in both normal and abnormal leg mechanical alignment settings.     

Italian healthcare workers' views on mandatory vaccination

Background  

Mandatory vaccination has contributed to the success of immunisation programmes but voluntary vaccination allows people to be responsible for their own health. There are benefits from both policies and the arguments between them remain subject to debate within and without the scientific community, both nationally and internationally. The aim of this study is to assess the opinions of those who actually work in the Vaccination Service.     

Quantitative computer-assisted osteodensitometry in total hip arthroplasty

Several factors can cause bone loss and fixation failure following total hip arthroplasty (THA), including polyethylene wear debris, implant micromotion and stress shielding. Various techniques have been used in an effort to detect bone density loss in vivo, all with varying success. Quantitative computed tomography (qCT)-assisted osteodensitometry has been shown to be useful in assessing the in vivo structural bone changes after THA. It has a high resolution, accuracy and reproducibility, thereby making it a useful tool for research purposes, and it is able to differentiate between cortical and cancellous bone structures and assess the bone/implant interface. This technique also provides valuable information about the pattern of stress shielding which occurs around the prosthesis and can show early bony changes, which may prove informative about the quality of implant fixation and surrounding bone adaptation. In conjunction with finite-element analysis, qCT is able to generate accurate patient-specific meshes on which to model implants and their effect on bone remodelling. This technology can be useful to predict bone remodelling and the quality of implant fixation using prostheses with different design and/or biomaterials. In the future, this tool could be used for pre-clinical validation of new implants before their introduction in the market-place.

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Résumé Plusieurs facteurs peuvent causer une perte osseuse et la faillite de la fixation après une arthroplastie totale de la hanche. Ils incluent les débris de polyéthylène, la micromobilité des implants et le transfert de contraintes. Plusieurs techniques ont été utilisées pour détecter la perte de densité osseuse, avec des succés variés. L’ostéodensitométrie quantitative par scanner s’est montrée utile dans l’étude in vivo des modifications structurales osseuses après arthroplastie totale de la hanche. Elle a une haute résolution, une précision et une reproductibilité qui en font un outil approprié pour la recherche. L’ostéodensitométrie quantitative peut différencier l’os cortical et l’os spongieux, étudier l’interface os-implant et donner des informations sur le modèle de déviation des contraintes qui surviennent autour d’une prothèse. Elle peut montrer précocement des modifications osseuses, ce qui renseigne sur la qualité de la fixation des implants et l’adaptation de l’os voisin. En conjonction avec l’analyse par éléments finis elle peut générer un maillage précis spécifique du patient permettant l’étude de modèles d’implants et leur effet sur le remodelage osseux. Cette technologie peut être utile pour prévoir le remodelage osseux et la qualité de la fixation pour des prothèses de différentes formes et/ou matériaux. Dans le future cet outil pourra être utilisé pour la validation pré-clinique de nouveaux implants avant leur introduction sur le marché.

     

Homozygous and compound heterozygous mutations in the ATP6V1B1 gene in patients with renal tubular acidosis and sensorineural hearing loss

Mohebbi N, Vargas‐Poussou R, Hegemann SCA, Schuknecht B, Kistler AD, Wüthrich RP, Wagner CA. Homozygous and compound heterozygous mutations in the ATP6V1B1 gene in patients with renal tubular acidosis and sensorineural hearing loss. Distal renal tubular acidosis (dRTA) is characterized by the inability to excrete acid in the renal collecting ducts resulting in inappropriately alkaline urine and hyperchloremic (normal anion gap) metabolic acidosis in the context of a normal (or near‐normal) glomerular filtration rate. Inborn dRTA can be due to autosomal dominant or recessive gene defects. Clinical symptoms vary from mild acidosis, incidental detection of kidney stones or renal tract calcification to severe findings such as failure to thrive, severe metabolic acidosis, and nephrocalcinosis. The majority of patients with recessive dRTA present with sensorineural hearing loss (SNHL). Few cases with abnormal widening of the vestibular aqueduct have been described with dRTA. Mutations in three different genes have been identified, namely SLC4A1, ATP6V1B1, and ATP6V0A4. Patients with mutations in the ATP6V1B1 proton pump subunit develop dRTA and in most of the cases sensorineural hearing loss early in childhood. We present two patients from two different and non‐consanguineous families with dRTA and SNHL. Direct sequencing of the ATP6V1B1 gene revealed that one patient harbors two homozygous mutations and the other one is a compound heterozygous. To our knowledge, this is the first case in the literature describing homozygosity in the same dRTA gene on both alleles.