In vitro survival of cells derived from isodiploid uniparental half embryos of the mouse after aggregation with normal embryos

Summary Fertilized mouse eggs, heterozygous for two allozymes of glucose phosphate isomerase (GPI) were bisected, and the resulting half eggs were diploidized with cytochalasin B. After separate aggregation with normal embryos carrying a third allozyme of GPI, the resulting chimaeras were kept in culture up to 10 days. The majority grew out on the culture dish during this period. By GPI analysis, 7.7% of the embryos were found to be chimaeric. Both types of uniparental cells, from gynogenetic and from androgenetic half eggs, were capable of surviving in chimaeras in vitro. These results are comparable with published data obtained by using uniparental embryos generated by micromanipulation.     

Inflammatory periproctal and anorectal conditions in HIV infections

Twenty-nine homosexual patients with anorectal symptoms were investigated by sigmoidoscopy. Nineteen patients were anti-HIV antibody positive; in this group severe haemorrhagic proctitis was diagnosed in seven cases and purulent cryptitis with abscess formation and fistulation in three cases. All patients showed a reduced OKT 4/OKT 8 ratio (below 1.0). It is concluded that the immunodeficient condition of HIV patients is the essential pathogenic factor for the occurrence of the anorectal inflammations observed.     

Aldosterone antagonists. 1. Synthesis and activities of 6 beta,7 beta:15 beta,16 beta-dimethylene steroidal spirolactones

Several derivatives of the highly active aldosterone antagonists dihydrospirorenone (2) and spirorenone (3) were synthesized. The purpose of these efforts was to prepare compounds exhibiting reduced endocrinological properties with the same or better aldosterone antagonistic activity than that of spirorenone. The 1 alpha,2 alpha-methylene derivative 20 has a similar aldosterone antagonistic potency compared to that of spirorenone but does not show decreased endocrinological side effects. Other substituents as in compounds 4-11, 15-19, and 21 sharply decreased the aldosterone antagonistic activity of 2 or 3, respectively.     

Treatment of gonorrhea in dermatological practice. Results of a survey

A questionnaire on the treatment of gonorrhea was sent to 1,560 dermato-venereologists practicing in the Federal Republic of Germany. Nine hundred sixty dermatologists returned the questionnaire. More than 60% of them examine less than 50 patients with gonorrhea per year; 44% treated the gonorrhea with spectinomycin, 42% with penicillin. Single dose treatment is used by approximately half of the dermatologists, but only 4% use single dose treatment with penicillin/probenecid. One case of lethal allergic shock to penicillin is reported to have occurred in 1980.     

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