Angulation and prominence of the posterior slope of the eminence of the temporomandibular joint in relation to disc position.

A steep articular eminence has been proposed as an aetiological factor in internal derangement of the temporomandibular joint. Arthrograms of 20 joints each of three groups, superior disc position (SDP), anterior disc displacement with reduction (ADDWR) and anterior disc displacement without reduction (ADDWOR) were compared. No correlation was found between a steep articular eminence and anterior disc displacement. The posterior slope of the articular eminence of the joints with ADDWOR was significantly less prominent than in those with SDP and ADDWR.     

Mutans streptococci in saliva and interdental spaces after topical applications of an antibacterial varnish in schoolchildren

The effect of a chlorhexidine-containing varnish on the levels of mutans streptococci in saliva and in interdental spaces was investigated in 33 15-year-old schoolchildren. Each child was treated with an active (1% chlorhexidine; 1% thymol) and a placebo varnish preparation following the split-mouth technique. The varnishes were applied twice in small amounts into upper interdental areas with a 2-d interval. Mutans streptococci in saliva and interdental plaque was sampled and enumerated during 3 months. The results showed an immediate reduction of the number of interdental mutans streptococci on both test and placebo side after the varnish applications. The levels, however, were significantly lower in the test quadrants compared with the placebo-treated sides after 8, 30 and 90 d. Thus, the findings indicate a slower recolonization in interdental spaces treated with the active preparation. The levels of mutans streptococci in saliva were significantly reduced 1 and 3 months after varnish treatment, suggesting a long-term effect of the antibacterial varnish.     

Ultrastructure of monolayer cultures of pancreatic islet cells

Summary Isolated islet cells from obese-hyperglycemic mice and normal guinea-pigs were culturedin vitro for periods of up to 7 days. Ultrastructural investigation showed that the cultured cells retained the morphologic characteristics of islet cellsin vivo. ₂-cells and -cells could be identified in explants from both obese-hyperglycemic mice and normal guinea-pigs. The most distinctive feature of the cultured islet cells was a marked degranulation of the -cells of both species. The degree of degranulation was not correlated with the glucose concentration in the incubation medium.This work was supported by the Swedish Medical Research Council (B69-12X-2297-02 and B69-12X-109-05), the United States Public Health Service (AM-12535) and the Medical Faculty, University of Uppsala.     

Radiographic and clinical estimation of endodontic treatment need

Abstract The purpose of this investigation was to examine the relationship between the need for endodontic treatment found by radiographic examination, and the actual treatment received by patients in an undergraduate endodontic clinic. The material consisted of 610 teeth in 387 patients. The need for endodontic treatment was detected in radiographs in 39% of the teeth, during operative procedures in 39%, and during the planning for prosthetic treatment in 22% of the cases. The relationship among these three means of detection was found to vary with age and tooth type. However, in all groups, less than half the actual need for endodontic treatment was revealed by radiographic examination alone.     

High-level bacterial secretion of single-chain alphabeta T-cell receptors

While numerous antibody-antigen systems have been structurally characterized, studies of structurally analogous T-cell receptor MHC systems have lagged behind largely due to the lack of a general TCR expression system. Efforts to develop bacterial systems have resulted in low yields (< 0.5 mg/l) of active material which is prone to proteolysis and aggregation. Here we report a strategy to secrete folded, soluble single chain T-cell receptors (scTCR) in the Escherichia coli periplasm using three representative alphabeta TCRs (172.10, 1934.4/c19 and 2B4). Shake flask yields between 0.5 and 30 mg/l active, purified material were attained for all TCRs studied and found to depend on the introduction of solubility-increasing amino acid substitutions, skp chaperone co-expression and C-terminal fusion to a human kappa constant domain in the context of a tightly regulated expression vector. This system will greatly enable crystallographic, thermodynamic and other biophysical analyses of TCRs which require large quantities of homogeneous material.     

Dental digital radiography: a survey of quality aspects

The aim was to evaluate the experiences of Swedish general dental practitioners (GDPs) with digital radiography and their opinion on the same, particularly regarding quality issues. A letter was sent to all GDPs in private care in Region Sk?ne, Sweden, asking whether they used digital radiography (n=513). The response rate was 79%. The number of private GDPs who replied that they used digital radiography was 106. The Public Dental Service in Region Sk?ne listed 33 GDPs who worked with digital radiography. Based on these answers, a questionnaire was sent to the GDPs working with digital radiography (n=139). The questionnaire comprised 27 questions about the dentists, the system of intra-oral digital radiography, and the GDPs' experiences of and opinions on issues regarding image quality and quality control. The response rate to the questionnaire was 94%. Almost all, 92%, worked with charge-coupled device (CCD) and complementary metal oxide semiconductor (CMOS) sensors. Most GDPs were satisfied with their digital radiographic system. The majority (65%) experienced problems. Detector failure and trouble with the software were common. The GDPs wrote that they used lower exposure times in digital radiography than traditional film radiography. The estimated reduction in exposure time was said to be between 51% and 75%. Thirty-five per cent continued to use film parallel with digital radiography. The answers indicated that less than half of the equipment (40%) underwent quality control. Quality controls, when conducted, were undertaken once or twice a year, mainly by technicians from the companies that had sold the digital equipment. Based on the results of the questionnaire, there seems to be a need to improve the maintenance and the quality of digital radiography. It is also important that the GDPs become more aware of the problems that can occur when a new technique is introduced and that they develop the skills to handle these problems.     

Effect of combined caries-preventive methods: a systematic review of controlled clinical trials

The aim of this systematic review was to evaluate the caries-preventive effect of combined caries-preventive methods, defined as two or more different interventions in combination, each expected to prevent dental caries. The Medline database was searched for articles published in the period January 1966 to June 2003. Twenty-four controlled studies met the inclusion criteria, and their value as evidence was assessed according to predetermined criteria. The level of evidence for the overall conclusion regarding each method was graded according to the protocol of the Swedish Council on Technology Assessment in Health Care. The scientific evidence for the combination of treatments involving fluoride that had a preventive effect on caries in children and adolescents was graded as moderate. However, for elderly patients the scientific evidence for the caries-preventive effect of different combinations of treatments was found to be incomplete. No conclusion could be drawn regarding the evidence for combinations of treatments being effective for groups at high caries risk, as the results from the identified clinical studies were conflicting.     

Caries-preventive effect of sodium fluoride mouthrinses: a systematic review of controlled clinical trials

The Swedish Council on Technology Assessment in Health Care launched a project group in 1999 to systematically review and evaluate the existing literature on different caries-preventive methods. The aim of this article was to report the findings concerning the caries-preventive effect of fluoride mouthrinses (FMRs) in various age groups, with special reference to background fluorides. A systematic search in electronic databases for literature published between 1966 and August 2003 was conducted with the inclusion criteria of a randomized or controlled clinical trial, at least 2 years' follow-up, and caries increment in the permanent dentition (DeltaDMFS/T) as endpoint. Out of 174 articles originally identified, 62 met the inclusion criteria. These studies were assessed independently by at least two reviewers and scored A-C according to predetermined criteria for methodology and performance. The measure of effect was the prevented fraction (PF) expressed as percent. The level of evidence was based on 25 articles. The results revealed limited evidence (evidence level 3) for the caries-preventive effect (PF 29%) of daily or weekly sodium fluoride rinses compared with placebo in permanent teeth of schoolchildren and adolescents with no additional fluoride exposure and for a caries-preventive effect on root caries in older adults. Inconclusive evidence (evidence level 4) was found regarding the effect of FMRs in schoolchildren and adolescents exposed to additional fluoride sources such as daily use of fluoride toothpaste. No firm support for the use of FMRs was disclosed in a small number of studies designed for patients at caries risk. Furthermore, no association between the frequency of the rinses and prevented fraction or saved surfaces per year was found. In conclusion, this systematic review suggests that sodium fluoride mouthrinses may have an anti-caries effect in children with limited background of fluoride exposure, while its additional effect in children with daily use of fluoride toothpaste could be questioned. The need for further clinical trials to elucidate the effect of FMRs in risk patients and older adults is emphasized.     

The irrelevant speech effect and working memory load

Irrelevant speech impairs the immediate serial recall of visually presented material. Previously, we have shown that the irrelevant speech effect (ISE) was associated with a relative decrease of regional blood flow in cortical regions subserving the verbal working memory, in particular the superior temporal cortex. In this extension of the previous study, the working memory load was increased and an increased activity as a response to irrelevant speech was noted in the dorsolateral prefrontal cortex. We suggest that the two studies together provide some basic insights as to the nature of the irrelevant speech effect. Firstly, no area in the brain can be ascribed as the single locus of the irrelevant speech effect. Instead, the functional neuroanatomical substrate to the effect can be characterized in terms of changes in networks of functionally interrelated areas. Secondly, the areas that are sensitive to the irrelevant speech effect are also generically activated by the verbal working memory task itself. Finally, the impact of irrelevant speech and related brain activity depends on working memory load as indicated by the differences between the present and the previous study. From a brain perspective, the irrelevant speech effect may represent a complex phenomenon that is a composite of several underlying mechanisms, which depending on the working memory load, include top-down inhibition as well as recruitment of compensatory support and control processes. We suggest that, in the low-load condition, a selection process by an inhibitory top-down modulation is sufficient, whereas in the high-load condition, at or above working memory span, auxiliary adaptive cognitive resources are recruited as compensation.